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Open data
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Basic information
Entry | Database: PDB / ID: 1ota | ||||||
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Title | E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN, P63 AT 295K | ||||||
![]() | Photoactive yellow protein | ||||||
![]() | SIGNALING PROTEIN / PYP | ||||||
Function / homology | ![]() photoreceptor activity / phototransduction / regulation of DNA-templated transcription / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Anderson, S. / Crosson, S. / Moffat, K. | ||||||
![]() | ![]() Title: Short hydrogen bonds in photoactive yellow protein. Authors: Anderson, S. / Crosson, S. / Moffat, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.4 KB | Display | ![]() |
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PDB format | ![]() | 48.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 425.3 KB | Display | ![]() |
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Full document | ![]() | 427.5 KB | Display | |
Data in XML | ![]() | 8.6 KB | Display | |
Data in CIF | ![]() | 11.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ot6C ![]() 1ot9C ![]() 1otbC ![]() 1otdC ![]() 1oteC ![]() 1otiC ![]() 2phyS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13887.591 Da / Num. of mol.: 1 / Mutation: E46Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-HC4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.52 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7 Details: ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 31, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→100 Å / Num. all: 42583 / Num. obs: 42583 / % possible obs: 86 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 9.7 % / Rmerge(I) obs: 0.051 / Net I/σ(I): 20 |
Reflection shell | Resolution: 1.1→1.14 Å / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 4.8 / % possible all: 71.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2PHY Resolution: 1.1→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 Stereochemistry target values: derived from 0.95A (110K) E46Q mutant ground state structure
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Refinement step | Cycle: LAST / Resolution: 1.1→20 Å
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Refine LS restraints |
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