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- PDB-1ot9: CRYOTRAPPED STATE IN WILD TYPE PHOTOACTIVE YELLOW PROTEIN, INDUCE... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ot9 | ||||||
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Title | CRYOTRAPPED STATE IN WILD TYPE PHOTOACTIVE YELLOW PROTEIN, INDUCED WITH CONTINUOUS ILLUMINATION AT 110K | ||||||
![]() | Photoactive yellow protein | ||||||
![]() | LUMINESCENT PROTEIN / PYP / cryotrapping | ||||||
Function / homology | ![]() photoreceptor activity / phototransduction / regulation of DNA-templated transcription / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Anderson, S. / Crosson, S. / Moffat, K. | ||||||
![]() | ![]() Title: Short hydrogen bonds in photoactive yellow protein. Authors: Anderson, S. / Crosson, S. / Moffat, K. | ||||||
History |
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Remark 300 | BIOMOLECULE Model 1 is associated with the photoactivated state and Model 2 is associated with the ...BIOMOLECULE Model 1 is associated with the photoactivated state and Model 2 is associated with the ground state of the protein. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121.4 KB | Display | ![]() |
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PDB format | ![]() | 100.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.6 KB | Display | ![]() |
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Full document | ![]() | 444 KB | Display | |
Data in XML | ![]() | 17.8 KB | Display | |
Data in CIF | ![]() | 25 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ot6C ![]() 1otaC ![]() 1otbC ![]() 1otdC ![]() 1oteC ![]() 1otiC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Number of models | 2 |
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Components
#1: Protein | Mass: 13888.575 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-HC4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.33 % Description: Author collected the first dataset in the dark, then a second dataset was collected on the same crystal after inducing a photostationary state with continuous illumination. The structure ...Description: Author collected the first dataset in the dark, then a second dataset was collected on the same crystal after inducing a photostationary state with continuous illumination. The structure was refined by the application of difference refinement to both datasets. |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 Details: ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
-Data collection
Diffraction |
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 26, 2001 | |||||||||||||||
Radiation |
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Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 1→100 Å / Num. all: 54493 / Num. obs: 54493 / % possible obs: 98.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 15.8 % / Rmerge(I) obs: 0.057 / Net I/σ(I): 30.8 | |||||||||||||||
Reflection shell | Resolution: 1→1.04 Å / Rmerge(I) obs: 0.568 / Mean I/σ(I) obs: 7.2 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: derived from 1.0A 110K ground state structure
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Refinement step | Cycle: LAST / Resolution: 1→20 Å
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Refine LS restraints |
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