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Open data
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Basic information
Entry | Database: PDB / ID: 1mex | ||||||
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Title | Antibody Catalysis of a Bimolecular Cycloaddition Reaction | ||||||
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![]() | IMMUNE SYSTEM / immunoglobulin fold | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / Chem-RAC![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Heine, A. / Wilson, I.A. | ||||||
![]() | ![]() Title: Antibody Catalysis of a Bimolecular Cycloaddition Reaction Authors: Heine, A. / Wilson, I.A. | ||||||
History |
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Remark 999 | SEQRES Author states the sequence should correspond to other antibody FAB sequences listed in the ...SEQRES Author states the sequence should correspond to other antibody FAB sequences listed in the Kabat and Wu "Sequence of proteins of immunological interest". In addition, author states residues 133 and 134 of chain H were refined as ALA, since there was no conclusive side chain density. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 197.9 KB | Display | ![]() |
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PDB format | ![]() | 163.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 23625.949 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 22790.404 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
#3: Chemical | ChemComp-RAC / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.89 Å3/Da / Density % sol: 35 % |
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Crystal grow | Temperature: 295.5 K / Method: vapor diffusion, sitting drop / pH: 7 Details: MPEG2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
-Data collection
Diffraction | Mean temperature: 84 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 6, 2001 / Details: single crystal bent |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96482 Å / Relative weight: 1 |
Reflection | Resolution: 1.25→20 Å / Num. all: 112412 / Num. obs: 112412 / % possible obs: 99.5 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Rsym value: 0.043 / Net I/σ(I): 27.4 |
Reflection shell | Resolution: 1.25→1.28 Å / Mean I/σ(I) obs: 1.9 / Num. unique all: 7422 / Rsym value: 0.638 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure: ![]() Details: ANISOTROPIC SCALING APPLIED BY THE METHOD OF PARKIN, MOEZZI & HOPE, J.APPL.CRYST.28(1995)53-56 ANISOTROPIC REFINEMENT REDUCED FREE R
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Refine analyze | Num. disordered residues: 12 / Occupancy sum hydrogen: 3128 / Occupancy sum non hydrogen: 3842 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.25→10 Å
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Refine LS restraints |
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