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- PDB-1a6t: FAB FRAGMENT OF MAB1-IA MONOCLONAL ANTIBODY TO HUMAN RHINOVIRUS 1... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1a6t | ||||||
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Title | FAB FRAGMENT OF MAB1-IA MONOCLONAL ANTIBODY TO HUMAN RHINOVIRUS 14 NIM-IA SITE | ||||||
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![]() | IMMUNOGLOBULIN / NEUTRALIZES HUMAN RHINOVIRUS / IGG1 | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Che, Z. / Smith, T.J. | ||||||
![]() | ![]() Title: Antibody-mediated neutralization of human rhinovirus 14 explored by means of cryoelectron microscopy and X-ray crystallography of virus-Fab complexes. Authors: Che, Z. / Olson, N.H. / Leippe, D. / Lee, W.M. / Mosser, A.G. / Rueckert, R.R. / Baker, T.S. / Smith, T.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 165.4 KB | Display | ![]() |
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PDB format | ![]() | 135.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 398.8 KB | Display | ![]() |
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Full document | ![]() | 433.2 KB | Display | |
Data in XML | ![]() | 21.8 KB | Display | |
Data in CIF | ![]() | 32.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1forS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | THERE ARE TWO FABS IN THE ASYMMETRIC UNIT. THE LIGHT AND HEAVY CHAINS ARE CALLED A AND B, RESPECTIVELY, FOR THE FIRST AND C AND D FOR THE SECOND. |
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Components
#1: Antibody | Mass: 22697.986 Da / Num. of mol.: 2 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: MONOCLONAL ANTIBODY / Source: (natural) ![]() ![]() #2: Antibody | Mass: 23294.027 Da / Num. of mol.: 2 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: MONOCLONAL ANTIBODY / Source: (natural) ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.43 % | |||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, sitting drop / pH: 6 Details: SITTING DROP METHOD USING 18-22% PEG8000, 0.1 M SODIUM PHOSPHATE BUFFER (PH 6.0), AND 1% 2-METHYL-2,4-PENTANEDIOL WITH AN FAB1 CONCENTRATION OF 18 MG/ML., vapor diffusion - sitting drop | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: Sep 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→30 Å / Num. obs: 29406 / % possible obs: 91.7 % / Observed criterion σ(I): 2 / Redundancy: 2.4 % / Rmerge(I) obs: 0.042 / Rsym value: 0.042 / Net I/σ(I): 3700 |
Reflection shell | Resolution: 2.6→2.64 Å / Redundancy: 2 % / Rmerge(I) obs: 0.15 / Mean I/σ(I) obs: 581 / Rsym value: 0.15 / % possible all: 89.2 |
Reflection shell | *PLUS % possible obs: 89.2 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1FOR Resolution: 2.7→6 Å / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Cross valid method: THROUGHOUT / σ(F): 0 / Details: STILL BEING REFINED
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Displacement parameters | Biso mean: 23.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.81 Å / Total num. of bins used: 6 / % reflection obs: 92 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
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