+Open data
-Basic information
Entry | Database: PDB / ID: 1mim | ||||||
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Title | IGG FAB FRAGMENT (CD25-BINDING) | ||||||
Components | (CHIMERIC SDZ CHI621) x 2 | ||||||
Keywords | IMMUNOGLOBULIN / C REGION | ||||||
Function / homology | Function and homology information IgD immunoglobulin complex / IgA immunoglobulin complex / IgM immunoglobulin complex / IgE immunoglobulin complex / CD22 mediated BCR regulation / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation ...IgD immunoglobulin complex / IgA immunoglobulin complex / IgM immunoglobulin complex / IgE immunoglobulin complex / CD22 mediated BCR regulation / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / immunoglobulin mediated immune response / Scavenging of heme from plasma / antigen binding / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCGR3A-mediated phagocytosis / B cell receptor signaling pathway / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / blood microparticle / adaptive immune response / Potential therapeutics for SARS / immune response / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.6 Å | ||||||
Authors | Mikol, V. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1996 Title: Structure of the fab fragment of SDZ CHI621: a chimeric antibody against CD25. Authors: Mikol, V. #1: Journal: Transplantation / Year: 1995 Title: Prolonged Action of a Chimeric Interleukin-2 Receptor (Cd25) Monoclonal Antibody Used in Cadaveric Renal Transplantation Authors: Amlot, P.L. / Rawlings, E. / Fernando, O.N. / Griffin, P.J. / Heinrich, G. / Schreier, M.H. / Castaigne, J.P. / Moore, R. / Sweny, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mim.cif.gz | 96 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1mim.ent.gz | 72.5 KB | Display | PDB format |
PDBx/mmJSON format | 1mim.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1mim_validation.pdf.gz | 432 KB | Display | wwPDB validaton report |
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Full document | 1mim_full_validation.pdf.gz | 447.3 KB | Display | |
Data in XML | 1mim_validation.xml.gz | 19 KB | Display | |
Data in CIF | 1mim_validation.cif.gz | 25.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mi/1mim ftp://data.pdbj.org/pub/pdb/validation_reports/mi/1mim | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 22882.338 Da / Num. of mol.: 1 / Fragment: IGG FAB Source method: isolated from a genetically manipulated source Details: MURINE ANTI-CD25 MONOCLONAL ANTIBODY, RFT5, CHIMERIZED WITH THE CONSTANT REGIONS OF HUMAN 1GG1 HEAVY AND KAPPA LIGHT CHAINS Source: (gene. exp.) Homo sapiens (human) Fragment: CONSTANT REGIONS OF 1GG1 HEAVY AND KAPPA LIGHT CHAINS References: UniProt: P01834 |
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#2: Antibody | Mass: 23144.826 Da / Num. of mol.: 1 / Fragment: IGG FAB Source method: isolated from a genetically manipulated source Details: MURINE ANTI-CD25 MONOCLONAL ANTIBODY, RFT5, CHIMERIZED WITH THE CONSTANT REGIONS OF HUMAN 1GG1 HEAVY AND KAPPA LIGHT CHAINS Source: (gene. exp.) Homo sapiens (human) Fragment: CONSTANT REGIONS OF 1GG1 HEAVY AND KAPPA LIGHT CHAINS References: GenBank: 9857755 |
#3: Water | ChemComp-HOH / |
Has protein modification | Y |
Nonpolymer details | THE WATER MOLECULE 2 WAS POSITIONED ON A STRONG ELECTRON DENSITY PEAK WHICH WAS OBSERVED BETWEEN ...THE WATER MOLECULE 2 WAS POSITIONED |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.37 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.6→15 Å / Num. obs: 14331 / % possible obs: 90.6 % / Observed criterion σ(I): 2 |
Reflection | *PLUS Num. measured all: 32477 / Rmerge(I) obs: 0.057 |
-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.6→8 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 2.6→8 Å
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Refine LS restraints |
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Xplor file |
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