+Open data
-Basic information
Entry | Database: PDB / ID: 1lvy | ||||||
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Title | PORCINE ELASTASE | ||||||
Components | ELASTASE | ||||||
Keywords | SERINE PROTEASE / HYDROLASE / ZYMOGEN / PANCREAS | ||||||
Function / homology | Function and homology information pancreatic elastase / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 1.87 Å | ||||||
Authors | Schiltz, M. / Prange, T. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1997 Title: High-pressure krypton gas and statistical heavy-atom refinement: a successful combination of tools for macromolecular structure determination. Authors: Schiltz, M. / Shepard, W. / Fourme, R. / Prange, T. / de la Fortelle, E. / Bricogne, G. #1: Journal: Acta Crystallogr.,Sect.B / Year: 1988 Title: Structure of Native Porcine Pancreatic Elastase at 1.65 A Resolutions Authors: Meyer, E. / Cole, G. / Radhakrishnan, R. / Epp, O. #2: Journal: J.Mol.Biol. / Year: 1978 Title: The Atomic Structure of Crystalline Porcine Pancreatic Elastase at 2.5 A Resolution: Comparisons with the Structure of Alpha-Chymotrypsin Authors: Sawyer, L. / Shotton, D.M. / Campbell, J.W. / Wendell, P.L. / Muirhead, H. / Watson, H.C. #3: Journal: Nature / Year: 1970 Title: Amino-Acid Sequence of Porcine Pancreatic Elastase and its Homologies with Other Serine Proteinases Authors: Shotton, D.M. / Hartley, B.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lvy.cif.gz | 58.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lvy.ent.gz | 43.8 KB | Display | PDB format |
PDBx/mmJSON format | 1lvy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1lvy_validation.pdf.gz | 430.7 KB | Display | wwPDB validaton report |
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Full document | 1lvy_full_validation.pdf.gz | 442.2 KB | Display | |
Data in XML | 1lvy_validation.xml.gz | 13 KB | Display | |
Data in CIF | 1lvy_validation.cif.gz | 17 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lv/1lvy ftp://data.pdbj.org/pub/pdb/validation_reports/lv/1lvy | HTTPS FTP |
-Related structure data
Related structure data | 3estS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 25928.031 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: PANCREAS / References: UniProt: P00772, pancreatic elastase |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
Sequence details | THE RESIDUE NUMBERING SCHEME USED FOR ELASTASE WAS CHOSEN TO MAXIMIZE HOMOLOGY WITH THE NUMBERING ...THE RESIDUE NUMBERING SCHEME USED FOR ELASTASE WAS CHOSEN TO MAXIMIZE HOMOLOGY WITH THE NUMBERING FOR CHYMOTRYPS |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.41 % | ||||||||||||||||||
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Crystal grow | pH: 5.5 / Details: CRYSTALLIZED IN NA2SO4 SOLUTION, pH 5.5 | ||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / Method: batch method | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW21B / Wavelength: 0.863 |
Detector | Type: J.HENDRIX,A.LENTFER / Detector: 'BLACK BOX' PROTOTYPE IMAGE PLATE / Date: Jul 14, 1995 / Details: IR-COATED CYLINDRICAL MIR |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.863 Å / Relative weight: 1 |
Reflection | Resolution: 1.87→30.4 Å / Num. obs: 17752 / % possible obs: 92.9 % / Observed criterion σ(I): 3 / Redundancy: 4.2 % / Biso Wilson estimate: 14.75 Å2 / Rmerge(I) obs: 0.022 / Net I/σ(I): 121 |
Reflection shell | Resolution: 1.87→1.97 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.05 / Mean I/σ(I) obs: 63.9 / % possible all: 78.6 |
Reflection | *PLUS Num. measured all: 74579 |
Reflection shell | *PLUS % possible obs: 78.6 % / Rmerge(I) obs: 0.05 |
-Processing
Software |
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Refinement | Method to determine structure: SIRAS Starting model: PDB ENTRY 3EST Resolution: 1.87→10 Å / Cross valid method: FREE-R / σ(F): 0
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Displacement parameters | Biso mean: 15.13 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.11 Å / Luzzati sigma a obs: 0.12 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.87→10 Å
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Refine LS restraints |
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