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Yorodumi- PDB-1esa: DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN TH... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1esa | ||||||
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Title | DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE | ||||||
Components | PORCINE PANCREATIC ELASTASE | ||||||
Keywords | HYDROLASE(SERINE PROTEINASE) | ||||||
Function / homology | Function and homology information pancreatic elastase / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.65 Å | ||||||
Authors | Ding, X. / Rasmussen, B. / Petsko, G.A. / Ringe, D. | ||||||
Citation | Journal: Biochemistry / Year: 1994 Title: Direct structural observation of an acyl-enzyme intermediate in the hydrolysis of an ester substrate by elastase. Authors: Ding, X. / Rasmussen, B.F. / Petsko, G.A. / Ringe, D. #1: Journal: Acta Crystallogr.,Sect.B / Year: 1988 Title: Structure of Native Procine Pancreatic Elastase at 1.65 Angstroms Resolution Authors: Meyer, E. / Cole, G. / Radhakrishnan, R. / Epp, O. | ||||||
History |
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Remark 700 | SHEET THE SHEETS PRESENTED AS *S1* AND *S2* ON SHEET RECORDS BELOW ARE ACTUALLY SIX-STRANDED BETA- ...SHEET THE SHEETS PRESENTED AS *S1* AND *S2* ON SHEET RECORDS BELOW ARE ACTUALLY SIX-STRANDED BETA-BARRELS. THIS IS REPRESENTED BY SEVEN-STRANDED SHEETS IN WHICH THE FIRST AND LAST STRAND OF EACH SHEET ARE IDENTICAL. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1esa.cif.gz | 57 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1esa.ent.gz | 44.9 KB | Display | PDB format |
PDBx/mmJSON format | 1esa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/es/1esa ftp://data.pdbj.org/pub/pdb/validation_reports/es/1esa | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 25928.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / References: UniProt: P00772, pancreatic elastase | ||||
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#2: Chemical | ChemComp-CA / | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Sequence details | THE RESIDUE NUMBERING SCHEME FOR THE PROTEIN IS SEQUENTIAL STARTING WITH VAL 16 AND ENDING WITH ASN ...THE RESIDUE NUMBERING SCHEME FOR THE PROTEIN IS SEQUENTIAL | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 42.97 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 2 ℃ / pH: 5 / Method: unknown / Details: Sawyer, L., (1978) J. Mol. Biol., 118, 137. | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.65 Å / Lowest resolution: 23 Å / Num. all: 18435 / Num. obs: 16146 / % possible obs: 95 % |
-Processing
Software |
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Refinement | Resolution: 1.65→10 Å / Rfactor obs: 0.19 / σ(F): 1 | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→10 Å
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Refinement | *PLUS Rfactor obs: 0.19 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS | ||||||||||||
Refine LS restraints | *PLUS
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