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Yorodumi- PDB-1gwp: STRUCTURE OF THE N-TERMINAL DOMAIN OF THE MATURE HIV-1 CAPSID PROTEIN -
+Open data
-Basic information
Entry | Database: PDB / ID: 1gwp | |||||||||
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Title | STRUCTURE OF THE N-TERMINAL DOMAIN OF THE MATURE HIV-1 CAPSID PROTEIN | |||||||||
Components | GAG POLYPROTEINGroup-specific antigen | |||||||||
Keywords | VIRAL PROTEIN / HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 CAPSID PROTEIN / AMIMO-TERMINAL CORE DOMAIN / HIV-1 CA-151 / VIRUS CAPSID PROTEIN / VIRUS MATURATION | |||||||||
Function / homology | Function and homology information viral budding via host ESCRT complex / ISG15 antiviral mechanism / host multivesicular body / viral nucleocapsid / host cell nucleus / structural molecule activity / host cell plasma membrane / virion membrane / RNA binding / zinc ion binding / membrane Similarity search - Function | |||||||||
Biological species | HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 | |||||||||
Method | SOLUTION NMR / torsion angle dynamics | |||||||||
Authors | Tang, C. / Gitti, R.K. / Lee, B.M. / Walker, J. / Summers, M.F. / Yoo, S. / Sundquist, W.I. | |||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2002 Title: Structure of the N-Terminal 283-Residue Fragment of the Immature HIV-1 Gag Polyprotein Authors: Tang, C. / Ndassa, Y. / Summers, M.F. #1: Journal: Science / Year: 1996 Title: Structure of the Amino-Terminal Core Domain of the HIV-1 Capsid Protein. Authors: Gitti, R.K. / Lee, B.M. / Walker, J. / Summers, M.F. / Yoo, S. / Sundquist, W.I. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gwp.cif.gz | 908.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gwp.ent.gz | 791.1 KB | Display | PDB format |
PDBx/mmJSON format | 1gwp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gw/1gwp ftp://data.pdbj.org/pub/pdb/validation_reports/gw/1gwp | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16716.189 Da / Num. of mol.: 1 / Fragment: AMINO-TERMINAL CORE DOMAIN RESIDUES 132 - 282 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (NEW YORK-5 ISOLATE) Strain: PNL4-3 / Cell line: CLONE 12 / Plasmid: PET11A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P12493 |
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Sequence details | GENBANK PID G902799 FOR GAG POLYPROTEI |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: HSQC HNCA, HN(CO)CA, HNCO, HNHA HMQC-NOESY-HSQC, HSQC-NOESY-HSQC, HMQC-NOESY-HMQC |
-Sample preparation
Sample conditions | Ionic strength: 10 mM / pH: 5.5 / Pressure: 1 atm / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: TORSION ANGLE DYNAMICS FOLLOWED BY ENERGY MINIMIZATION. GUNTERT, P., MUMENTHALER, C. WUTHRICH, K. (1997). TORSION ANGLE DYNAMICS FOR NMR STRUCTURE CALCULATION WITH THE NEW PROGRAM DYANA. J. ...Details: TORSION ANGLE DYNAMICS FOLLOWED BY ENERGY MINIMIZATION. GUNTERT, P., MUMENTHALER, C. WUTHRICH, K. (1997). TORSION ANGLE DYNAMICS FOR NMR STRUCTURE CALCULATION WITH THE NEW PROGRAM DYANA. J. MOL. BIOL. 273, 283-298. | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST TARGET FUNCTION / Conformers calculated total number: 100 / Conformers submitted total number: 20 |