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- PDB-1skn: THE BINDING DOMAIN OF SKN-1 IN COMPLEX WITH DNA: A NEW DNA-BINDIN... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1skn | ||||||
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Title | THE BINDING DOMAIN OF SKN-1 IN COMPLEX WITH DNA: A NEW DNA-BINDING MOTIF | ||||||
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![]() | TRANSCRIPTION/DNA / COMPLEX (TRANSCRIPTION FACTOR-DNA) / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | ![]() : / positive regulation of cellular response to manganese ion / : / response to paraquat / Factors involved in megakaryocyte development and platelet production / positive regulation of cellular response to oxidative stress / : / mesendoderm development / endodermal cell fate specification / multicellular organism development ...: / positive regulation of cellular response to manganese ion / : / response to paraquat / Factors involved in megakaryocyte development and platelet production / positive regulation of cellular response to oxidative stress / : / mesendoderm development / endodermal cell fate specification / multicellular organism development / embryonic digestive tract development / embryonic pattern specification / response to superoxide / cell fate specification / Hsp70 protein binding / determination of adult lifespan / RNA polymerase II transcription regulatory region sequence-specific DNA binding / regulation of translation / response to heat / response to oxidative stress / sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / host cell nucleus / positive regulation of gene expression / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / endoplasmic reticulum / positive regulation of transcription by RNA polymerase II / mitochondrion / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rupert, P.B. / Daughdrill, G.W. / Bowerman, B. / Matthews, B.W. | ||||||
![]() | ![]() Title: A new DNA-binding motif in the Skn-1 binding domain-DNA complex. Authors: Rupert, P.B. / Daughdrill, G.W. / Bowerman, B. / Matthews, B.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48 KB | Display | ![]() |
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PDB format | ![]() | 30.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.7 KB | Display | ![]() |
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Full document | ![]() | 466.2 KB | Display | |
Data in XML | ![]() | 9.6 KB | Display | |
Data in CIF | ![]() | 11.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: DNA chain | Mass: 4528.961 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 4649.033 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 10996.467 Da / Num. of mol.: 1 / Fragment: BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#4: Chemical | ChemComp-LDA / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PROTEIN WAS CRYSTALLIZED FROM 8% PEG 1000 50 MM BIS-TRIS, PH 6.0, 100-150 MM LICL, 2MM LDAO., VAPOR DIFFUSION, HANGING DROP, temperature 277.00K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal | *PLUS Density % sol: 45 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 15, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.5→20 Å / Num. obs: 7202 / % possible obs: 94.8 % / Redundancy: 6.9 % / Biso Wilson estimate: 38.4 Å2 / Rmerge(I) obs: 0.038 / Rsym value: 0.038 |
Reflection shell | Resolution: 2.5→2.57 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.116 / % possible all: 100 |
Reflection | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 20 Å |
Reflection shell | *PLUS % possible obs: 100 % |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Details: THE SHORT CONTACTS LISTED IN REMARK 500 ARE THE RESULT OF THE LAURYLDIMETHYLAMINEOXIDE (LDA) MOLECULE MODELED ON A TWO-FOLD AXIS AT HALF OCCUPANCY. THIS LDA MOLECULE WAS EXCLUDED FROM MAKING ...Details: THE SHORT CONTACTS LISTED IN REMARK 500 ARE THE RESULT OF THE LAURYLDIMETHYLAMINEOXIDE (LDA) MOLECULE MODELED ON A TWO-FOLD AXIS AT HALF OCCUPANCY. THIS LDA MOLECULE WAS EXCLUDED FROM MAKING BAD CONTACTS WITH A SYMMETRY-RELATED COPY OF ITSELF IN THE TNT REFINEMENT PROTOCOL.
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Solvent computation | Solvent model: TNT 5F / Bsol: 110 Å2 / ksol: 0.8 e/Å3 | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5F / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.225 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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