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Yorodumi- PDB-1enr: CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH ZINC AND CALCIUM ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1enr | ||||||
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Title | CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH ZINC AND CALCIUM HAVING A ZINC ION BOUND IN THE S1 SITE AND A CALCIUM ION BOUND IN THE S2 SITE | ||||||
Components | CONCANAVALIN A | ||||||
Keywords | PLANT LECTIN (AGGLUTININ) / CONCANAVALIN A / PLANT LECTIN / AGGLUTININ | ||||||
Function / homology | Function and homology information regulation of defense response to virus / D-mannose binding / defense response / metal ion binding Similarity search - Function | ||||||
Biological species | Canavalia ensiformis (jack bean) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.83 Å | ||||||
Authors | Bouckaert, J. / Loris, R. / Poortmans, F. / Wyns, L. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1996 Title: Sequential structural changes upon zinc and calcium binding to metal-free concanavalin A. Authors: Bouckaert, J. / Poortmans, F. / Wyns, L. / Loris, R. #1: Journal: Proteins / Year: 1995 Title: Crystallographic Structure of Metal-Free Concanavalin a at 2.5 Angstrom Resolution Authors: Bouckaert, J. / Loris, R. / Poortmans, F. / Wyns, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1enr.cif.gz | 61.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1enr.ent.gz | 44.8 KB | Display | PDB format |
PDBx/mmJSON format | 1enr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1enr_validation.pdf.gz | 412.4 KB | Display | wwPDB validaton report |
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Full document | 1enr_full_validation.pdf.gz | 413.5 KB | Display | |
Data in XML | 1enr_validation.xml.gz | 12 KB | Display | |
Data in CIF | 1enr_validation.cif.gz | 16.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/en/1enr ftp://data.pdbj.org/pub/pdb/validation_reports/en/1enr | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Details | THE ASYMMETRIC UNIT CONTAINS ONE MONOMER OF 237 AMINO ACIDS. |
-Components
#1: Protein | Mass: 25622.385 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: ZINC/CALCIUM COMPLEX / Source: (natural) Canavalia ensiformis (jack bean) / References: UniProt: P02866 |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-ZN / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.9 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7. | ||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 46.9 % | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.1 / Method: unknown | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: Jun 13, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→11.7 Å / Num. obs: 21804 / % possible obs: 99.4 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.062 |
Reflection | *PLUS Highest resolution: 1.8 Å / Num. obs: 21738 / Num. measured all: 158149 |
-Processing
Software |
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Refinement | Resolution: 1.83→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 16 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.15 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.83→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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