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- EMDB-9688: Cryo-EM structure of full Echovirus 6 particle at PH 5.5 -

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Basic information

Entry
Database: EMDB / ID: EMD-9688
TitleCryo-EM structure of full Echovirus 6 particle at PH 5.5
Map dataCryo-EM structure of full Echovirus 6 particle at PH 5.5
Sample
  • Virus: Echovirus E6
    • Protein or peptide: Capsid protein VP1
    • Protein or peptide: Capsid protein VP2
    • Protein or peptide: Capsid protein VP3
    • Protein or peptide: Capsid protein VP4
  • Ligand: SPHINGOSINE
KeywordsVIRUS
Biological speciesEchovirus E6
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsGao GF / Liu S
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of SciencesXDB29010000 China
CitationJournal: Cell / Year: 2019
Title: Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.
Authors: Xin Zhao / Guigen Zhang / Sheng Liu / Xiangpeng Chen / Ruchao Peng / Lianpan Dai / Xiao Qu / Shihua Li / Hao Song / Zhengrong Gao / Pengfei Yuan / Zhiheng Liu / Changyao Li / Zifang Shang / ...Authors: Xin Zhao / Guigen Zhang / Sheng Liu / Xiangpeng Chen / Ruchao Peng / Lianpan Dai / Xiao Qu / Shihua Li / Hao Song / Zhengrong Gao / Pengfei Yuan / Zhiheng Liu / Changyao Li / Zifang Shang / Yan Li / Meifan Zhang / Jianxun Qi / Han Wang / Ning Du / Yan Wu / Yuhai Bi / Shan Gao / Yi Shi / Jinghua Yan / Yong Zhang / Zhengde Xie / Wensheng Wei / George F Gao /
Abstract: Enterovirus B (EV-B), a major proportion of the genus Enterovirus in the family Picornaviridae, is the causative agent of severe human infectious diseases. Although cellular receptors for ...Enterovirus B (EV-B), a major proportion of the genus Enterovirus in the family Picornaviridae, is the causative agent of severe human infectious diseases. Although cellular receptors for coxsackievirus B in EV-B have been identified, receptors mediating virus entry, especially the uncoating process of echovirus and other EV-B remain obscure. Here, we found that human neonatal Fc receptor (FcRn) is the uncoating receptor for major EV-B. FcRn binds to the virus particles in the "canyon" through its FCGRT subunit. By obtaining multiple cryo-electron microscopy structures at different stages of virus entry at atomic or near-atomic resolution, we deciphered the underlying mechanisms of enterovirus attachment and uncoating. These structures revealed that different from the attachment receptor CD55, binding of FcRn to the virions induces efficient release of "pocket factor" under acidic conditions and initiates the conformational changes in viral particle, providing a structural basis for understanding the mechanisms of enterovirus entry.
History
DepositionOct 19, 2018-
Header (metadata) releaseMay 15, 2019-
Map releaseMay 15, 2019-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6iln
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6iln
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9688.png

Downloads & links

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Map

FileDownload / File: emd_9688.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM structure of full Echovirus 6 particle at PH 5.5
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.35 Å/pix.
x 360 pix.
= 486. Å		1.35 Å/pix.
x 360 pix.
= 486. Å		1.35 Å/pix.
x 360 pix.
= 486. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.35 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.05
Minimum - Maximum-0.20753902 - 0.32142994
Average (Standard dev.)0.002147805 (±0.019851016)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 486.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.351.351.35
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z486.000486.000486.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.2080.3210.002

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Supplemental data

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Sample components

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Entire : Echovirus E6

EntireName: Echovirus E6
Components
  • Virus: Echovirus E6
    • Protein or peptide: Capsid protein VP1
    • Protein or peptide: Capsid protein VP2
    • Protein or peptide: Capsid protein VP3
    • Protein or peptide: Capsid protein VP4
  • Ligand: SPHINGOSINE

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Supramolecule #1: Echovirus E6

SupramoleculeName: Echovirus E6 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#4 / NCBI-ID: 12062 / Sci species name: Echovirus E6 / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Capsid protein VP1

MacromoleculeName: Capsid protein VP1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Echovirus E6
Molecular weightTheoretical: 31.364988 KDa
SequenceString: VVRVADTMPS GPSNSESIPA LTAAETGHTS QVVPSDTIQT RHVRNFHVRS ESSVENFLSR SACVYIVEYK TRDDTPDKMY DSWVINTRQ VAQLRRKLEF FTYVRFDVEV TFVITSVQDD STRQNTDTPA LTHQIMYVPP GGPIPQAVDD YNWQTSTNPS V FWTEGNAP ...String:
VVRVADTMPS GPSNSESIPA LTAAETGHTS QVVPSDTIQT RHVRNFHVRS ESSVENFLSR SACVYIVEYK TRDDTPDKMY DSWVINTRQ VAQLRRKLEF FTYVRFDVEV TFVITSVQDD STRQNTDTPA LTHQIMYVPP GGPIPQAVDD YNWQTSTNPS V FWTEGNAP PRMSIPFMSV GNAYSNFYDG WSHFSQTGVY GFNTLNNMGK LYFRHVNDKT ISPITSKVRI YFKPKHVKAW VP RPPRLCE YTHKDNVDFE PKGVTTSRTQ LTISNSTH

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Macromolecule #2: Capsid protein VP2

MacromoleculeName: Capsid protein VP2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Echovirus E6
Molecular weightTheoretical: 28.06452 KDa
SequenceString: SDRVRSITLG NSTITTQESA NVVVGYGVWP DYLSDEEATA EDQPTQPDVA TCRFYTLDSV SWMKESQGWW WKFPDALRDM GLFGQNMQY HYLGRSGYTI HVQCNASKFH QGCLLVVCVP EAEMGAANIN EKINREHLSN GEVANTFSGT KSSNTNDVQQ A VFNAGMGV ...String:
SDRVRSITLG NSTITTQESA NVVVGYGVWP DYLSDEEATA EDQPTQPDVA TCRFYTLDSV SWMKESQGWW WKFPDALRDM GLFGQNMQY HYLGRSGYTI HVQCNASKFH QGCLLVVCVP EAEMGAANIN EKINREHLSN GEVANTFSGT KSSNTNDVQQ A VFNAGMGV AVGNLTIFPH QWINLRTNNC ATIVMPYINS VPMDNMFRHY NFTLMIIPFA KLDYAAGSST YIPITVTVAP MC AEYNGLR LAGHQ

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Macromolecule #3: Capsid protein VP3

MacromoleculeName: Capsid protein VP3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Echovirus E6
Molecular weightTheoretical: 26.378936 KDa
SequenceString: GLPVMNTPGS NQFLTSDDYQ SPTAMPQFDV TPEMNIPGEV KNLMEIAEVD SVVPVNNVNE NVNSLEAYRI PVHSVTETGA QVFGFTLQP GADTVMERTL LGEILNYYAN WSGSIKLTFM YCGSAMATGK FLLAYSPPGA GVPKNRREAM LGTHIIWDIG L QSSCVLCV ...String:
GLPVMNTPGS NQFLTSDDYQ SPTAMPQFDV TPEMNIPGEV KNLMEIAEVD SVVPVNNVNE NVNSLEAYRI PVHSVTETGA QVFGFTLQP GADTVMERTL LGEILNYYAN WSGSIKLTFM YCGSAMATGK FLLAYSPPGA GVPKNRREAM LGTHIIWDIG L QSSCVLCV PWISQTHYRF VSKDIYTDAG FITCWYQTSI VVPAEVQNQS VILCFVSACN DFSVRLLRDS PFVRQTAFYQ

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Macromolecule #4: Capsid protein VP4

MacromoleculeName: Capsid protein VP4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Echovirus E6
Molecular weightTheoretical: 7.224855 KDa
SequenceString:
GAQVSTQKTG AHETSLSASG NSIHYTNINY YKDAASNSAN RQDFTQDPGK FTEPVKDIMV KSLPALN

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Macromolecule #5: SPHINGOSINE

MacromoleculeName: SPHINGOSINE / type: ligand / ID: 5 / Number of copies: 1 / Formula: SPH
Molecular weightTheoretical: 299.492 Da
Chemical component information

ChemComp-SPH:
SPHINGOSINE / Sphingosine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 5.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 4321
DetailsCryo-EM structure of full Echovirus 6 particle at PH 5.5
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-6iln:
Cryo-EM structure of full Echovirus 6 particle at PH 5.5

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