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- EMDB-3307: Structure of the human TAF-less PIC in the closed state -

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Entry
Database: EMDB / ID: EMD-3307
TitleStructure of the human TAF-less PIC in the closed state
Map dataReconstruction of the human TAF-less PIC
Sample
  • Sample: Human TAF-less PIC bound to super core promoter DNA in the closed state
  • Protein or peptide: TATA-box-binding protein
  • Protein or peptide: General transcription factor IIA
  • Protein or peptide: General transcription factor IIB
  • Protein or peptide: General transcription factor IIF
  • Protein or peptide: RNA polymerase II
  • Protein or peptide: General transcription factor IIE
  • Protein or peptide: General transcription factor IIH
  • Protein or peptide: Transcription factor IIS
  • DNA: Super core promoter
KeywordsTFIID / TFIIA / transcription / RNA polymerase II / general transcription factors / preinitiation complex / core promoter / DNA binding
Function / homology
Function and homology information


microfibril binding / MMXD complex / core TFIIH complex portion of holo TFIIH complex / Cytosolic iron-sulfur cluster assembly / central nervous system myelin formation / RNA Polymerase III Chain Elongation / positive regulation of core promoter binding / RNA polymerase II core complex assembly / RNA Polymerase III Transcription Termination / RNA polymerase II CTD heptapeptide repeat S5 kinase activity ...microfibril binding / MMXD complex / core TFIIH complex portion of holo TFIIH complex / Cytosolic iron-sulfur cluster assembly / central nervous system myelin formation / RNA Polymerase III Chain Elongation / positive regulation of core promoter binding / RNA polymerase II core complex assembly / RNA Polymerase III Transcription Termination / RNA polymerase II CTD heptapeptide repeat S5 kinase activity / positive regulation of mitotic recombination / hair cell differentiation / hair follicle maturation / transcription factor TFIIE complex / nucleotide-excision repair factor 3 complex / RNA polymerase transcription factor SL1 complex / nucleotide-excision repair, preincision complex assembly / meiotic sister chromatid cohesion / phosphatase activator activity / ventricular system development / snRNA transcription by RNA polymerase II / CAK-ERCC2 complex / RNA polymerase III general transcription initiation factor activity / TFIIF-class transcription factor complex binding / regulation of transcription by RNA polymerase I / transcription factor TFIIK complex / transcriptional start site selection at RNA polymerase II promoter / RNA polymerase I core promoter sequence-specific DNA binding / UV protection / RPAP3/R2TP/prefoldin-like complex / transcription factor TFIIF complex / embryonic cleavage / DNA 5'-3' helicase / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / transcription factor TFIIA complex / adult heart development / female germ cell nucleus / RNA Polymerase III Abortive And Retractive Initiation / regulation of cyclin-dependent protein serine/threonine kinase activity / Cytosolic sensors of pathogen-associated DNA / G protein-coupled receptor internalization / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / male pronucleus / cyclin-dependent protein serine/threonine kinase activator activity / female pronucleus / germinal vesicle / RNA polymerase II general transcription initiation factor binding / nuclear thyroid hormone receptor binding / [RNA-polymerase]-subunit kinase / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / transcription preinitiation complex / RNA Polymerase I Transcription Termination / MicroRNA (miRNA) biogenesis / cyclin-dependent protein serine/threonine kinase regulator activity / regulation of mitotic cell cycle phase transition / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / DNA 3'-5' helicase / LRR domain binding / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / protein acetylation / cell division site / erythrocyte maturation / 3'-5' DNA helicase activity / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / hematopoietic stem cell proliferation / spinal cord development / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / acetyltransferase activity / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / bone mineralization / mRNA Splicing - Minor Pathway / PIWI-interacting RNA (piRNA) biogenesis / aryl hydrocarbon receptor binding / viral transcription / RNA polymerase II complex binding / ATPase activator activity / intrinsic apoptotic signaling pathway by p53 class mediator / TFIIB-class transcription factor binding / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / DNA topological change / Processing of Capped Intron-Containing Pre-mRNA / nuclear-transcribed mRNA catabolic process / RNA Polymerase I Transcription Initiation / regulation of G1/S transition of mitotic cell cycle
Similarity search - Function
Transcription elongation factor, TFIIS / Transcription elongation factor, IIS-type / Transcription factor S-II (TFIIS), central domain / Domain in the central regions of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor S-II, central domain / Transcription elongation factor S-II, central domain superfamily / TFIIS central domain profile. / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Transcription factor TFIIE alpha subunit, C-terminal ...Transcription elongation factor, TFIIS / Transcription elongation factor, IIS-type / Transcription factor S-II (TFIIS), central domain / Domain in the central regions of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor S-II, central domain / Transcription elongation factor S-II, central domain superfamily / TFIIS central domain profile. / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Transcription factor TFIIE alpha subunit, C-terminal / C-terminal general transcription factor TFIIE alpha / TFIIS N-terminal domain profile. / Transcription factor IIS, N-terminal / TFIIS helical bundle-like domain / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain / TFIIH C1-like domain / TFIIH p62 subunit, N-terminal / TFIIH subunit Tfb1/GTF2H1 / TFIIH p62 subunit, N-terminal domain / BSD domain / CyclinH/Ccl1 / BSD domain superfamily / BSD domain / BSD domain profile. / domain in transcription factors and synapse-associated proteins / RAD3/XPD family / Helicase XPB/Ssl2 / ERCC3/RAD25/XPB helicase, C-terminal domain / Helicase XPB/Ssl2, N-terminal domain / Helicase conserved C-terminal domain / ERCC3/RAD25/XPB C-terminal helicase / Helical and beta-bridge domain / Helical and beta-bridge domain / Transcription factor TFIIH subunit p52/Tfb2 / Transcription factor Tfb2, C-terminal domain / Transcription factor Tfb2 / Transcription factor Tfb2 (p52) C-terminal domain / Transcription factor TFIIE beta subunit, DNA-binding domain / TFIIH subunit TTDA/Tfb5 / Transcription initiation factor TFIIE, beta subunit / TFB5-like superfamily / Cyclin-dependent kinase 7 / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / Transcription factor TFIIH complex subunit Tfb5 / TFA2 Winged helix domain 2 / TFIIE beta central core DNA-binding domain profile. / Transcription factor TFIIH complex subunit Tfb5 / ATP-dependent helicase Rad3/Chl1-like / : / Helicase-like, DEXD box c2 type / DEAD2 / DEAD_2 / DEXDc2 / Helicase superfamily 1/2, DinG/Rad3-like / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / HELICc2 / ATP-dependent helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-type / Helicase C-terminal domain / Superfamilies 1 and 2 helicase ATP-binding type-2 domain profile. / Transcription initiation factor IIE subunit alpha, N-terminal / Transcription factor TFE/TFIIEalpha HTH domain / TFIIEalpha/SarR/Rpc3 HTH domain / Transcription factor E / TFIIE alpha subunit / TFE/IIEalpha-type HTH domain profile. / Transcription initiation factor IIE / Transcription factor IIA, alpha/beta subunit / Cyclin, C-terminal domain 2 / Transcription factor IIA, alpha/beta subunit / Cyclin C-terminal domain / Transcription factor IIA, alpha/beta subunit / Transcription initiation factor IIA, gamma subunit / Transcription factor IIA, alpha-helical domain / Transcription factor IIA, beta-barrel / Transcription initiation factor IIA, gamma subunit, C-terminal / Transcription initiation factor IIA, gamma subunit, N-terminal / Transcription initiation factor IIA, gamma subunit, helical domain / Transcription initiation factor IIA, gamma subunit / Transcription initiation factor IIF, beta subunit / TFIIF beta subunit, HTH domain / TFIIF, beta subunit, N-terminal / TFIIF, beta subunit HTH domain / TFIIF, beta subunit N-terminus / Transcription initiation factor IIF, alpha subunit / Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) / Transcription Factor IIF, Rap30/Rap74, interaction / Transcription factor TFIIB, cyclin-like domain / Transcription factor TFIIB, conserved site / Transcription factor TFIIB repeat
Similarity search - Domain/homology
DNA-directed RNA polymerase II subunit RPB4 / General transcription factor IIF subunit 2 / General transcription and DNA repair factor IIH helicase subunit XPD / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / General transcription and DNA repair factor IIH helicase/translocase subunit XPB / TATA-box-binding protein / Transcription elongation factor A protein 1 / DNA-directed RNA polymerase II subunit RPB1 / General transcription factor IIE subunit 1 ...DNA-directed RNA polymerase II subunit RPB4 / General transcription factor IIF subunit 2 / General transcription and DNA repair factor IIH helicase subunit XPD / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / General transcription and DNA repair factor IIH helicase/translocase subunit XPB / TATA-box-binding protein / Transcription elongation factor A protein 1 / DNA-directed RNA polymerase II subunit RPB1 / General transcription factor IIE subunit 1 / Transcription initiation factor IIE subunit beta / DNA-directed RNA polymerase II subunit RPB2 / General transcription factor IIH subunit 1 / General transcription factor IIF subunit 1 / DNA-directed RNA polymerase II subunit RPB9 / Cyclin-dependent kinase 7 / Cyclin-H / CDK-activating kinase assembly factor MAT1 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB11-a / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB7 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Transcription initiation factor IIB / General transcription factor IIH subunit 2 / General transcription factor IIH subunit 3 / General transcription factor IIH subunit 5 / General transcription factor IIH subunit 4
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.2 Å
AuthorsLouder RK / He Y / Lopez-Blanco JR / Fang J / Chacon P / Nogales E
Citation
Journal: Nature / Year: 2016
Title: Structure of promoter-bound TFIID and model of human pre-initiation complex assembly.
Authors: Robert K Louder / Yuan He / José Ramón López-Blanco / Jie Fang / Pablo Chacón / Eva Nogales /
Abstract: The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the ...The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation.
#1: Journal: To Be Published
Title: Structural basis of eukaryotic transcription initiation
Authors: He Y / Yan C / Fang J / Inouye C / Tjian R / Ivanov I / Nogales E
History
DepositionJan 27, 2016-
Header (metadata) releaseFeb 3, 2016-
Map releaseMar 30, 2016-
UpdateApr 13, 2016-
Current statusApr 13, 2016Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.045
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  • Surface view with fitted model
  • Atomic models: PDB-5iy6
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6o9l
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3307.png

Downloads & links

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Map

FileDownload / File: emd_3307.map.gz / Format: CCP4 / Size: 26.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationReconstruction of the human TAF-less PIC
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.64 Å/pix.
x 192 pix.
= 506.88 Å		2.64 Å/pix.
x 192 pix.
= 506.88 Å		2.64 Å/pix.
x 192 pix.
= 506.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.64 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.045 / Movie #1: 0.045
Minimum - Maximum-0.11061411 - 0.21786748
Average (Standard dev.)0.0006074 (±0.00719656)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions192192192
Spacing192192192
CellA=B=C: 506.88 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.642.642.64
M x/y/z192192192
origin x/y/z0.0000.0000.000
length x/y/z506.880506.880506.880
α/β/γ90.00090.00090.000
start NX/NY/NZ-300-64
NX/NY/NZ6161129
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS192192192
D min/max/mean-0.1110.2180.001

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Supplemental data

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Sample components

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Entire : Human TAF-less PIC bound to super core promoter DNA in the closed...

EntireName: Human TAF-less PIC bound to super core promoter DNA in the closed state
Components
  • Sample: Human TAF-less PIC bound to super core promoter DNA in the closed state
  • Protein or peptide: TATA-box-binding protein
  • Protein or peptide: General transcription factor IIA
  • Protein or peptide: General transcription factor IIB
  • Protein or peptide: General transcription factor IIF
  • Protein or peptide: RNA polymerase II
  • Protein or peptide: General transcription factor IIE
  • Protein or peptide: General transcription factor IIH
  • Protein or peptide: Transcription factor IIS
  • DNA: Super core promoter

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Supramolecule #1000: Human TAF-less PIC bound to super core promoter DNA in the closed...

SupramoleculeName: Human TAF-less PIC bound to super core promoter DNA in the closed state
type: sample / ID: 1000 / Number unique components: 9
Molecular weightTheoretical: 1.36 MDa

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Macromolecule #1: TATA-box-binding protein

MacromoleculeName: TATA-box-binding protein / type: protein_or_peptide / ID: 1 / Name.synonym: TBP / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 38 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceUniProtKB: TATA-box-binding protein / InterPro: TATA-box binding protein

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Macromolecule #2: General transcription factor IIA

MacromoleculeName: General transcription factor IIA / type: protein_or_peptide / ID: 2 / Name.synonym: TFIIA / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 27 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #3: General transcription factor IIB

MacromoleculeName: General transcription factor IIB / type: protein_or_peptide / ID: 3 / Name.synonym: TFIIB / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 35 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #4: General transcription factor IIF

MacromoleculeName: General transcription factor IIF / type: protein_or_peptide / ID: 4 / Name.synonym: TFIIF / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 87 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #5: RNA polymerase II

MacromoleculeName: RNA polymerase II / type: protein_or_peptide / ID: 5 / Name.synonym: Pol II / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: No
Source (natural)Organism: Homo sapiens (human) / Strain: HeLa / synonym: Human / Organelle: Nucleus / Location in cell: Nuclear pellet
Molecular weightTheoretical: 550 KDa

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Macromolecule #6: General transcription factor IIE

MacromoleculeName: General transcription factor IIE / type: protein_or_peptide / ID: 6 / Name.synonym: TFIIE / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 82 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #7: General transcription factor IIH

MacromoleculeName: General transcription factor IIH / type: protein_or_peptide / ID: 7 / Name.synonym: TFIIH / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: No
Source (natural)Organism: Homo sapiens (human) / synonym: Human / Organelle: Nucleus / Location in cell: Nuclear extract
Molecular weightTheoretical: 450 KDa

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Macromolecule #8: Transcription factor IIS

MacromoleculeName: Transcription factor IIS / type: protein_or_peptide / ID: 8 / Name.synonym: TFIIS / Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #9: Super core promoter

MacromoleculeName: Super core promoter / type: dna / ID: 9 / Name.synonym: SCP
Details: The super core promoter is a composite sequence combining promoter motifs from several strong promoters from humans and D. melanogaster.
Classification: DNA / Structure: DOUBLE HELIX / Synthetic?: Yes
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 56 KDa
SequenceString:
GAAGGGCGCC TATAAAAGGG GGTGGGGGCG CGTTCGTCCT CAGTCGCGAT CGAACACTCG AGCCGAGCAG ACGTGCCTAC GGACCATGG

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.05 mg/mL
BufferpH: 7.9
Details: 10 mM HEPES, 5% glycerol, 10 mM MgCl2, 50 mM KCl, 1 mM DTT, 0.05% NP-40
GridDetails: Amorphous continuous carbon over C-flat holey carbon support (4 um holes with 2 um spacing) on 400 mesh copper grid.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV
Method: Incubate 4 ul of sample on grid for 10 minutes, then blot for 4 seconds with force 15.

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Electron microscopy

MicroscopeFEI TITAN
DetailsThe camera was operated in counting mode with a dose rate of 8 electrons/pixel per second.
DateMay 8, 2014
Image recordingCategory: CCD / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Number real images: 855 / Average electron dose: 46 e/Å2
Details: Whole-micrograph drift correction was performed using MotionCorr before averaging the frames.
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 37879 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.0 µm
Sample stageSpecimen holder model: GATAN LIQUID NITROGEN

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Image processing

CTF correctionDetails: Each particle
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.2 Å / Resolution method: OTHER / Software - Name: CTFFIND3, RELION, Bsoft / Number images used: 24290
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

1nvp

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 2

Initial modelPDB ID:

1c9b

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 3

Initial modelPDB ID:

4bbr

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 4

Initial modelPDB ID:

4bbr

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 5

Initial modelPDB ID:

1f3u

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 6

Initial modelPDB ID:

1bby

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 7

Initial modelPDB ID:

3crv

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 8

Initial modelPDB ID:

1y1v

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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Atomic model buiding 9

Initial modelPDB ID:

3ndq

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5iy6:
Human holo-PIC in the closed state

PDB-6o9l:
Human holo-PIC in the closed state

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