+Open data
-Basic information
Entry | Database: PDB / ID: 1nvp | ||||||
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Title | HUMAN TFIIA/TBP/DNA COMPLEX | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / TRANSCRIPTION REGULATION / DNA / COMPLEX / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | Function and homology information RNA polymerase transcription factor SL1 complex / RNA polymerase III general transcription initiation factor activity / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / RNA Polymerase III Abortive And Retractive Initiation / transcription factor TFIIA complex / female germ cell nucleus / male pronucleus / female pronucleus ...RNA polymerase transcription factor SL1 complex / RNA polymerase III general transcription initiation factor activity / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / RNA Polymerase III Abortive And Retractive Initiation / transcription factor TFIIA complex / female germ cell nucleus / male pronucleus / female pronucleus / RNA polymerase II general transcription initiation factor binding / transcription preinitiation complex / RNA Polymerase I Transcription Termination / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase I Transcription Initiation / aryl hydrocarbon receptor binding / RNA polymerase II transcribes snRNA genes / TFIIB-class transcription factor binding / positive regulation of transcription initiation by RNA polymerase II / RNA polymerase II core promoter sequence-specific DNA binding / transcription by RNA polymerase III / core promoter sequence-specific DNA binding / RNA polymerase II preinitiation complex assembly / RNA Polymerase II Pre-transcription Events / TBP-class protein binding / SIRT1 negatively regulates rRNA expression / male germ cell nucleus / transcription initiation at RNA polymerase II promoter / DNA-templated transcription initiation / RNA Polymerase I Promoter Escape / euchromatin / mRNA transcription by RNA polymerase II / NoRC negatively regulates rRNA expression / B-WICH complex positively regulates rRNA expression / cell junction / spermatogenesis / DNA-binding transcription factor binding / Estrogen-dependent gene expression / Regulation of TP53 Activity through Phosphorylation / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / transcription cis-regulatory region binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein heterodimerization activity / chromatin / enzyme binding / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Bleichenbacher, M. / Tan, S. / Richmond, T.J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes. Authors: Bleichenbacher, M. / Tan, S. / Richmond, T.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1nvp.cif.gz | 113.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1nvp.ent.gz | 83.2 KB | Display | PDB format |
PDBx/mmJSON format | 1nvp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nv/1nvp ftp://data.pdbj.org/pub/pdb/validation_reports/nv/1nvp | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-DNA chain , 2 types, 2 molecules EF
#1: DNA chain | Mass: 5381.497 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 5034.261 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Transcription initiation factor IIA ... , 3 types, 3 molecules BCD
#4: Protein | Mass: 6486.258 Da / Num. of mol.: 1 / Fragment: N-terminal 58 amino acids Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2A1 OR TF2A1 / Production host: Escherichia coli (E. coli) / References: UniProt: P52655 |
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#5: Protein | Mass: 8687.350 Da / Num. of mol.: 1 / Fragment: c-terminal 76 amino acids Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2A1 OR TF2A1 / Production host: Escherichia coli (E. coli) / References: UniProt: P52655 |
#6: Protein | Mass: 12337.896 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GTF2A2 OR TF2A2 / Production host: Escherichia coli (E. coli) / References: UniProt: P52657 |
-Protein / Non-polymers , 2 types, 232 molecules A
#3: Protein | Mass: 20443.254 Da / Num. of mol.: 1 / Fragment: C-TERMINAL 181 AMINO ACIDS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TBP OR TFIID OR TF2D / Production host: Escherichia coli (E. coli) / References: UniProt: P20226 |
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#7: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.37 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG6000, lithium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 22 ℃ / pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→39 Å / Num. obs: 40386 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 5.6 % / Biso Wilson estimate: 30.5 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 25.6 | ||||||||||||||||||
Reflection shell | Resolution: 2.1→2.15 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.362 / Mean I/σ(I) obs: 4.1 / % possible all: 99.8 | ||||||||||||||||||
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 40 Å / Redundancy: 5.6 % / Num. measured all: 224980 | ||||||||||||||||||
Reflection shell | *PLUS % possible obs: 99.8 % / Redundancy: 3.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1→39 Å / Rfactor Rfree error: 0.004 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 Details: REFINED ALSO WITH REFMAC BY MURSHUDOV, VAGIN, DODSON. NO ELECTRON DENSITY VISIBLE FOR: RESIDUES 339, CHAIN A RESIDUES 2-8, CHAIN B RESIDUES 52-58, CHAIN B RESIDUES 301-329, CHAIN C RESIDUE ...Details: REFINED ALSO WITH REFMAC BY MURSHUDOV, VAGIN, DODSON. NO ELECTRON DENSITY VISIBLE FOR: RESIDUES 339, CHAIN A RESIDUES 2-8, CHAIN B RESIDUES 52-58, CHAIN B RESIDUES 301-329, CHAIN C RESIDUE 2, CHAIN D RESIDUES 100-109, CHAIN D SIDE CHAINS FOR RESIDUES K12 OF CHAIN B. SIDE CHAINS FOR RESIDUES D330, R363 OF CHAIN C. SIDE CHAINS FOR RESIDUES Q50, R51, R53, T85, Q86 OF CHAIN D.
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Displacement parameters | Biso mean: 58.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→39 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.014 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 40 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.322 / Rfactor Rwork: 0.29 |