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Structure paper

TitleNear-atomic resolution visualization of human transcription promoter opening.
Journal, issue, pagesNature, Vol. 533, Issue 7603, Page 359-365, Year 2016
Publish dateMay 19, 2016
AuthorsYuan He / Chunli Yan / Jie Fang / Carla Inouye / Robert Tjian / Ivaylo Ivanov / Eva Nogales /
PubMed AbstractIn eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the ...In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the start site for transcription by RNA polymerase II. Here we use cryo-electron microscropy (cryo-EM) to determine near-atomic resolution structures of the human PIC in a closed state (engaged with duplex DNA), an open state (engaged with a transcription bubble), and an initially transcribing complex (containing six base pairs of DNA-RNA hybrid). Our studies provide structures for previously uncharacterized components of the PIC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF. Comparison of the different structures reveals the sequential conformational changes that accompany the transition from each state to the next throughout the transcription initiation process. This analysis illustrates the key role of TFIIB in transcription bubble stabilization and provides strong structural support for a translocase activity of XPB.
External linksNature / PubMed:27193682 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 10.0 Å
Structure data

8131

5ivw

EMDB-8131, PDB-5ivw:
Human core TFIIH bound to DNA within the PIC
Method: EM (single particle) / Resolution: 10.0 Å

8132

5iy7

EMDB-8132, PDB-5iy7:
Human holo-PIC in the open state
Method: EM (single particle) / Resolution: 8.6 Å

8133

5iy8

EMDB-8133, PDB-5iy8:
Human holo-PIC in the initial transcribing state
Method: EM (single particle) / Resolution: 7.9 Å

8134

5iy9

EMDB-8134, PDB-5iy9:
Human holo-PIC in the initial transcribing state (no IIS)
Method: EM (single particle) / Resolution: 6.3 Å

8135

5iya

EMDB-8135, PDB-5iya:
Human core-PIC in the closed state
Method: EM (single particle) / Resolution: 5.4 Å

8136

5iyb

EMDB-8136, PDB-5iyb:
Human core-PIC in the open state
Method: EM (single particle) / Resolution: 3.9 Å

8137

5iyc

EMDB-8137, PDB-5iyc:
Human core-PIC in the initial transcribing state
Method: EM (single particle) / Resolution: 3.9 Å

8138

5iyd

EMDB-8138, PDB-5iyd:
Human core-PIC in the initial transcribing state (no IIS)
Method: EM (single particle) / Resolution: 3.9 Å

PDB-5iy6:
Human holo-PIC in the closed state
Method: ELECTRON MICROSCOPY / Resolution: 7.2 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • Human (human)
KeywordsTRANSCRIPTION/DNA / initiation / RNA polymerase II / human / TRANSCRIPTION-DNA complex / TRANSCRIPTION / TRANSFERASE/DNA / TRANSFERASE-DNA complex / TRANSFERASE/DNA/RNA / TRANSFERASE-DNA-RNA complex

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