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- EMDB-13769: CryoEM structure of Apoferritin at 2.6A from Tundra, 100kV microscope -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-13769 | |||||||||
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Title | CryoEM structure of Apoferritin at 2.6A from Tundra, 100kV microscope | |||||||||
![]() | Apoferritin data from Tundra, 100kV microscope with CetaF detector | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.6 Å | |||||||||
![]() | Karia D / Hlavenkova H / Koh A / Malinsky M / Yu L / Kotecha A | |||||||||
Funding support | 1 items
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![]() | ![]() Title: CryoEM structure of Apoferritin at 2.6A from Tundra, 100kV microscope Authors: Karia D / Hlavenkova H / Koh A / Malinsky M / Yu L / Kotecha A | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 9.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.3 KB 10.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.1 KB | Display | ![]() |
Images | ![]() | 215.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 351.6 KB | Display | ![]() |
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Full document | ![]() | 351.2 KB | Display | |
Data in XML | ![]() | 11 KB | Display | |
Data in CIF | ![]() | 14.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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EM raw data | ![]() Data size: 1.0 TB Data #1: Unaligned multiframe movies of ApoF from CETA-F camera [micrographs - multiframe]) |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Apoferritin data from Tundra, 100kV microscope with CetaF detector | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.0476 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Apoferritin
Entire | Name: Apoferritin |
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Components |
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-Supramolecule #1: Apoferritin
Supramolecule | Name: Apoferritin / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
Molecular weight | Theoretical: 480 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 4 mg/mL |
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Buffer | pH: 7.5 / Details: 20mM HEPES pH 7.5 and 300mM NaCl |
Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
Details | 4mg/ml protein in 20mM HEPES pH 7.5 and 300mM NaCl |
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Electron microscopy
Microscope | TFS TUNDRA |
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Image recording | Film or detector model: FEI CETA (4k x 4k) / Number grids imaged: 1 / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 100 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 1.6 mm / Nominal magnification: 180000 |
Sample stage | Specimen holder model: OTHER / Cooling holder cryogen: NITROGEN |