+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-51463 | |||||||||
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Title | ApoF at 2.2A resolution imaged at 0.55A/pixel on Falcon C | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Octahedral symmetry Tundra Falcon C / METAL BINDING PROTEIN | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.2 Å | |||||||||
Authors | Koh FA / Karia D / Yang W / Yu L / Plitzko J / Aricescu R / Lander GC / Greber B / Kotecha A | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Sub-3 A resolution structure 2 determination using single-particle 3 cryo-EM at 100 keV Authors: Karia D / Koh FA / Yang W / Cushing V / Basanta B / Mihaylov D / Khavnekar S / Vyroubal O / Malinsky M / Shanel O / Dolezal V / Plitzko JM / Yu L / Lander GC / Aricescu R / Greber B / Kotecha A | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_51463.map.gz | 16.9 MB | EMDB map data format | |
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Header (meta data) | emd-51463-v30.xml emd-51463.xml | 11.9 KB 11.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_51463_fsc.xml | 11.3 KB | Display | FSC data file |
Images | emd_51463.png | 156.3 KB | ||
Filedesc metadata | emd-51463.cif.gz | 3.9 KB | ||
Others | emd_51463_half_map_1.map.gz emd_51463_half_map_2.map.gz | 93.6 MB 93.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-51463 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-51463 | HTTPS FTP |
-Validation report
Summary document | emd_51463_validation.pdf.gz | 660.5 KB | Display | EMDB validaton report |
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Full document | emd_51463_full_validation.pdf.gz | 660 KB | Display | |
Data in XML | emd_51463_validation.xml.gz | 18.4 KB | Display | |
Data in CIF | emd_51463_validation.cif.gz | 24.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-51463 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-51463 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_51463.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.7425 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_51463_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_51463_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Apoferritin
Entire | Name: Apoferritin |
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Components |
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-Supramolecule #1: Apoferritin
Supramolecule | Name: Apoferritin / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Mus musculus (house mouse) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS TUNDRA |
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Image recording | Film or detector model: OTHER / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 100 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 0.6 µm / Nominal defocus min: 0.2 µm |