SASDF86: Human Galectin-10 (Tyr69Glu mutant) (Galectin-10 Tyr69Glu, Gal-10)

Basic information

• Entry: Database: SASBDB / ID: SASDF86

Sample

Human Galectin-10 (Tyr69Glu mutant)

• Galectin-10 Tyr69Glu (protein), Gal-10, Homo sapiens

Function / homology

Function:

regulation of activated T cell proliferation / regulation of T cell cytokine production / T cell apoptotic process / regulation of T cell anergy / : / carbohydrate binding / identical protein binding / cytosol

Domain/homology:

Galectin-like / Galactoside-binding lectin / Galectin / Galectin, carbohydrate recognition domain / Galactoside-binding lectin / Galactoside-binding lectin (galectin) domain profile. / Concanavalin A-like lectin/glucanase domain superfamily

Component:

Galectin-10

• Biological species: Homo sapiens (human)
• Citation: Journal: Science / Year: 2019; Title: Protein crystallization promotes type 2 immunity and is reversible by antibody treatment.; Authors: Emma K Persson / Kenneth Verstraete / Ines Heyndrickx / Elien Gevaert / Helena Aegerter / Jean-Michel Percier / Kim Deswarte / Koen H G Verschueren / Ann Dansercoer / Delphine Gras / Pascal Chanez / Claus Bachert / Amanda Gonçalves / Hanne Van Gorp / Hans De Haard / Christophe Blanchetot / Michael Saunders / Hamida Hammad / Savvas N Savvides / Bart N Lambrecht / ; Abstract: Although spontaneous protein crystallization is a rare event in vivo, Charcot-Leyden crystals (CLCs) consisting of galectin-10 (Gal10) protein are frequently observed in eosinophilic diseases, such as asthma. We found that CLCs derived from patients showed crystal packing and Gal10 structure identical to those of Gal10 crystals grown in vitro. When administered to the airways, crystalline Gal10 stimulated innate and adaptive immunity and acted as a type 2 adjuvant. By contrast, a soluble Gal10 mutein was inert. Antibodies directed against key epitopes of the CLC crystallization interface dissolved preexisting CLCs in patient-derived mucus within hours and reversed crystal-driven inflammation, goblet-cell metaplasia, immunoglobulin E (IgE) synthesis, and bronchial hyperreactivity (BHR) in a humanized mouse model of asthma. Thus, protein crystals may promote hallmark features of asthma and are targetable by crystal-dissolving antibodies.

Contact author

• Clement Blanchet
• Kenneth Verstraete

Models

• Model #2989: ; Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 11.2234292988744; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Human Galectin-10 (Tyr69Glu mutant) / Specimen concentration: 20 mg/ml
• Buffer: Name: 20 mM Hepes 150 NaCl / pH: 7.4

Entity #1642

Name: Gal-10 / Type: protein / Description: Galectin-10 Tyr69Glu / Formula weight: 16.613 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q05315
Sequence:

GHMSLLPVPY TEAASLSTGS TVTIKGRPLA CFLNEPYLQV DFHTEMKEES DIVFHFQVCF GRRVVMNSRE EGAWKQQVES KNMPFQDGQE FELSISVLPD KYQVMVNGQS SYTFDHRIKP EAVKMVQVWR DISLTKFNVS YLKR

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.8 mm
• Detector: Name: Eiger 4M / Pixsize x: 75 mm

Scan

Title: Galactin 10 / Measurement date: Feb 4, 2018 / Cell temperature: 15 °C / Exposure time: 1 sec. / Number of frames: 14 / Unit: 1/A /

	Min	Max
Q	0.0066	0.6089

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 470 /

	Min	Max
Q	0.007348	0.4816
P(R) point	1	470
R	0	81.5

Result

Type of curve: sec /

	Experimental	Porod
MW	30.2 kDa	28.7 kDa
Volume	-	46 nm3

	Guinier	P(R)	Guinier error
Forward scattering, I0	0.120254	-	-
Radius of gyration, Rg	2.147 nm	2.172 nm	0.01

	Min	Max
D	-	8.15
Guinier point	15	102