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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDE59 |
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![]() | Interleukin-1 receptor type 1, IL-1RI-ECD
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機能・相同性 | ![]() positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / positive regulation of neutrophil extravasation / interleukin-1 receptor activity / interleukin-1 binding / positive regulation of T-helper 1 cell cytokine production / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / NAD+ nucleosidase activity / NAD+ nucleotidase, cyclic ADP-ribose generating / platelet-derived growth factor receptor binding ...positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / positive regulation of neutrophil extravasation / interleukin-1 receptor activity / interleukin-1 binding / positive regulation of T-helper 1 cell cytokine production / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / NAD+ nucleosidase activity / NAD+ nucleotidase, cyclic ADP-ribose generating / platelet-derived growth factor receptor binding / interleukin-1-mediated signaling pathway / Interleukin-10 signaling / response to interleukin-1 / Interleukin-1 signaling / positive regulation of type II interferon production / transmembrane signaling receptor activity / regulation of inflammatory response / protease binding / Potential therapeutics for SARS / cell surface receptor signaling pathway / inflammatory response / immune response / external side of plasma membrane / extracellular region / membrane / plasma membrane 類似検索 - 分子機能 |
生物種 | ![]() |
![]() | ![]() タイトル: Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. 著者: Jiwan Ge / Soumya G Remesh / Michal Hammel / Si Pan / Andrew D Mahan / Shuying Wang / Xinquan Wang / ![]() ![]() ![]() 要旨: The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 ...The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 cytokines. The inter-domain flexibility of IL-1 receptors and its functional roles have not been fully elucidated. In this study, we used small-angle X-ray scattering to show that ligand-binding primary receptors and co-receptors in the family all have inherent inter-domain flexibility due to the D2/D3 linker. Variants of the IL-1RAcP and IL-18Rβ co-receptors with mutated D2/D3 linkers cannot form a cytokine-receptor complex and mediate signaling. Our analysis further revealed that these mutated co-receptors exhibited a changed conformational ensemble, suggesting that loss of function is due to the alteration of receptor dynamics. Taken together, our results demonstrate that the D2/D3 linker is a critical functional determinant of IL-1 receptor and underscore the important roles of the inter-domain flexibility in cytokine/receptor binding and signaling. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #2681 | ![]() タイプ: atomic / カイ2乗値: 1.2737678614 ![]() |
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モデル #2682 | ![]() タイプ: atomic / カイ2乗値: 1.2737678614 ![]() |
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試料
![]() | 名称: Interleukin-1 receptor type 1, IL-1RI-ECD / 試料濃度: 10 mg/ml |
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バッファ | 名称: 10mM HEPES, 150mM NaCl, 3% glycerol / pH: 7.2 |
要素 #1393 | 名称: IL-1R-1 / タイプ: protein / 記述: Interleukin-1 receptor type 1 / 分子量: 36.899 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P14778 配列: LEADKCKERE EKIILVSSAN EIDVRPCPLN PNEHKGTITW YKDDSKTPVS TEQASRIHQH KEKLWFVPAK VEDSGHYYCV VRNSSYCLRI KISAKFVENE PNLCYNAQAI FKQKLPVAGD GGLVCPYMEF FKNENNELPK LQWYKDCKPL LLDNIHFSGV KDRLIVMNVA ...配列: LEADKCKERE EKIILVSSAN EIDVRPCPLN PNEHKGTITW YKDDSKTPVS TEQASRIHQH KEKLWFVPAK VEDSGHYYCV VRNSSYCLRI KISAKFVENE PNLCYNAQAI FKQKLPVAGD GGLVCPYMEF FKNENNELPK LQWYKDCKPL LLDNIHFSGV KDRLIVMNVA EKHRGNYTCH ASYTYLGKQY PITRVIEFIT LEENKPTRPV IVSPANETME VDLGSQIQLI CNVTGQLSDI AYWKWNGSVI DEDDPVLGED YYSVENPANK RRSTLITVLN ISEIESRFYK HPFTCFAKNT HGIDAAYIQL IYPVHHHHHH |
-実験情報
ビーム | 設備名称: Advanced Light Source (ALS) 12.3.1 (SIBYLS) / 地域: Berkeley, CA / 国: USA ![]() | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus3 X 2M / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
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距離分布関数 P(R) |
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結果 | コメント: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; ...コメント: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.
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