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- SASDE59: Interleukin-1 receptor type 1, IL-1RI-ECD (Interleukin-1 receptor... -
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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDE59 |
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![]() | Interleukin-1 receptor type 1, IL-1RI-ECD
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Function / homology | ![]() positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / positive regulation of neutrophil extravasation / interleukin-1 receptor activity / interleukin-1 binding / positive regulation of T-helper 1 cell cytokine production / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / NAD+ nucleosidase activity / NAD+ nucleotidase, cyclic ADP-ribose generating / platelet-derived growth factor receptor binding ...positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / positive regulation of neutrophil extravasation / interleukin-1 receptor activity / interleukin-1 binding / positive regulation of T-helper 1 cell cytokine production / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / NAD+ nucleosidase activity / NAD+ nucleotidase, cyclic ADP-ribose generating / platelet-derived growth factor receptor binding / interleukin-1-mediated signaling pathway / Interleukin-10 signaling / response to interleukin-1 / Interleukin-1 signaling / positive regulation of type II interferon production / transmembrane signaling receptor activity / regulation of inflammatory response / protease binding / Potential therapeutics for SARS / cell surface receptor signaling pathway / inflammatory response / immune response / external side of plasma membrane / extracellular region / membrane / plasma membrane Similarity search - Function |
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![]() | ![]() Title: Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. Authors: Jiwan Ge / Soumya G Remesh / Michal Hammel / Si Pan / Andrew D Mahan / Shuying Wang / Xinquan Wang / ![]() ![]() ![]() Abstract: The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 ...The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 cytokines. The inter-domain flexibility of IL-1 receptors and its functional roles have not been fully elucidated. In this study, we used small-angle X-ray scattering to show that ligand-binding primary receptors and co-receptors in the family all have inherent inter-domain flexibility due to the D2/D3 linker. Variants of the IL-1RAcP and IL-18Rβ co-receptors with mutated D2/D3 linkers cannot form a cytokine-receptor complex and mediate signaling. Our analysis further revealed that these mutated co-receptors exhibited a changed conformational ensemble, suggesting that loss of function is due to the alteration of receptor dynamics. Taken together, our results demonstrate that the D2/D3 linker is a critical functional determinant of IL-1 receptor and underscore the important roles of the inter-domain flexibility in cytokine/receptor binding and signaling. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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External links
Related items in Molecule of the Month |
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-Models
Model #2681 | ![]() Type: atomic / Chi-square value: 1.2737678614 ![]() |
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Model #2682 | ![]() Type: atomic / Chi-square value: 1.2737678614 ![]() |
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Sample
![]() | Name: Interleukin-1 receptor type 1, IL-1RI-ECD / Specimen concentration: 10 mg/ml |
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Buffer | Name: 10mM HEPES, 150mM NaCl, 3% glycerol / pH: 7.2 |
Entity #1393 | Name: IL-1R-1 / Type: protein / Description: Interleukin-1 receptor type 1 / Formula weight: 36.899 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P14778 Sequence: LEADKCKERE EKIILVSSAN EIDVRPCPLN PNEHKGTITW YKDDSKTPVS TEQASRIHQH KEKLWFVPAK VEDSGHYYCV VRNSSYCLRI KISAKFVENE PNLCYNAQAI FKQKLPVAGD GGLVCPYMEF FKNENNELPK LQWYKDCKPL LLDNIHFSGV KDRLIVMNVA ...Sequence: LEADKCKERE EKIILVSSAN EIDVRPCPLN PNEHKGTITW YKDDSKTPVS TEQASRIHQH KEKLWFVPAK VEDSGHYYCV VRNSSYCLRI KISAKFVENE PNLCYNAQAI FKQKLPVAGD GGLVCPYMEF FKNENNELPK LQWYKDCKPL LLDNIHFSGV KDRLIVMNVA EKHRGNYTCH ASYTYLGKQY PITRVIEFIT LEENKPTRPV IVSPANETME VDLGSQIQLI CNVTGQLSDI AYWKWNGSVI DEDDPVLGED YYSVENPANK RRSTLITVLN ISEIESRFYK HPFTCFAKNT HGIDAAYIQL IYPVHHHHHH |
-Experimental information
Beam | Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS) City: Berkeley, CA / 国: USA ![]() | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus3 X 2M / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
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Distance distribution function P(R) |
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Result | Comments: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.
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