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- SASDCS7: TET12(1.10)SN-f9 -

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Entry
Database: SASBDB / ID: SASDCS7
SampleTET12(1.10)SN-f9
  • TET12(1.10)SN-f9 (protein), synthetic construct
Sourcesynthetic construct (others)
CitationJournal: Nat. Biotechnol. / Year: 2017
Title: Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.
Authors: Ajasja Ljubetič / Fabio Lapenta / Helena Gradišar / Igor Drobnak / Jana Aupič / Žiga Strmšek / Duško Lainšček / Iva Hafner-Bratkovič / Andreja Majerle / Nuša Krivec / Mojca Benčina / Tomaž Pisanski / Tanja Ćirković Veličković / Adam Round / José María Carazo / Roberto Melero / Roman Jerala
Contact authorJana Aupic (National Institute of Chemistry, Slovenia)

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Models

Model #1577
Type: atomic / Software: (9.16) / Radius of dummy atoms: 1.90 A
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: TET12(1.10)SN-f9 / Specimen concentration: 1.60-6.00 / Entity id: 803
BufferName: 20 mM Tris 150 mM NaCl 10% glycerol / pH: 7.5 / Sample id: 656
Entity #803Type: protein / Description: TET12(1.10)SN-f9 / Formula weight: 56.581 / Number of molecules: 1 / Source: synthetic construct
Sequence:
MLEEELKQLE EELQAIEEQL AQLQWKAQAR KEKLAQLKEK LSGSGPGSGS PEDEIQQLEE EISQLEQKNS ELKEKNQELK YGSGSGPGSG DIEQELERAK ESIRRLEQEV NQERSRMQYL QTLLEKSGSG PGSGQLEDKV EELLSKNYHL ENEVERLKKL VGSGSGPGSG LEEELKQLEE ELQAIEEQLA QLQWKAQARK EKLAQLKEKL SGSGPGSGSP EDEIQQLEEK NSQLKQEISQ LEEKNQELKY GSGSGPGSGQ LEDKVEELLS KNYHLENEVE RLKKLVGSGS GPGSGSPEDK ISQLKEKIQQ LKQENQQLEE ENSQLEYGSG SGPGSGSPED ENSQLEEKIS QLKQKNSELK EEIQQLEYGS GSGPGSGSPE DKISELKEEN QQLEQKIQQL KEENSQLEYG SGSGPGSGDI EQELERAKES IRRLEQEVNQ ERSRMQYLQT LLEKSGSGPG SGSPEDKNSE LKEEIQQLEE ENQQLEEKIS ELKYGLEHHH HHHHH

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Experimental information

BeamInstrument name: PETRA III P12 / Instrument city: Hamburg / Instrument country: Germany / Shape: Point / Type of source: X-ray synchrotronSynchrotron / Radiation wavelength: 0.124 / Dist spec to detc: 2
DetectorName: Pilatus 1M-W / Pixsize x: 0.172
Scan
Title: TET12(1.10)SN-f9 / Detc id: 1 / Measurement date: Jun 10, 2016 / Storage temperature: 1 / Cell temperature: 2 / Exposure time: 0.045 / Number of frames: 20 / Unit: 1/nm / Qmin: 0.2526 / Qmax: 6.7156 / Sample id: 656 / Result id: 697 / Beam id: 61
Distance distribution function P(R)
Intensity id: 697 / Software p of R: GNOM 5.0 / Number of points: 291 / Qmin: 0.252581 / Qmax: 1.83291 / P of R point min: 1 / P of R point max: 291 / Rmin: 0 / Rmax: 12.9
Result
SASBDB code: SASDCS7 / Experimental MW: 37 / MW standard: 37 / MW standard error: 2 / MW Porod: 9 / I0 from PR: 5027 / I0 from PR error: 2 / I0 from Guinier: 5021 / I0 from Guinier error: 6 / Rg from PR: 3.95 / Rg from PR error: 0.01 / Rg from Guinier: 3.87 / Rg from Guinier error: 0.05 / D max: 12.9 / Porod volume: 153 / Guinier point min: 3 / Guinier point max: 16 / Type of curve: single_conc / Dmax error: 2

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