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- SASDCX7: TET12(1.10)S-f5b -

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Entry
Database: SASBDB / ID: SASDCX7
SampleTET12(1.10)S-f5b
  • TET12(1.10)S-f5b (protein), synthetic construct
Sourcesynthetic construct (others)
CitationJournal: Nat. Biotechnol. / Year: 2017
Title: Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.
Authors: Ajasja Ljubetič / Fabio Lapenta / Helena Gradišar / Igor Drobnak / Jana Aupič / Žiga Strmšek / Duško Lainšček / Iva Hafner-Bratkovič / Andreja Majerle / Nuša Krivec / Mojca Benčina / Tomaž Pisanski / Tanja Ćirković Veličković / Adam Round / José María Carazo / Roberto Melero / Roman Jerala
Contact authorJana Aupic (National Institute of Chemistry, Slovenia)

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Structure visualization

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Models

Model #1588
Type: atomic / Software: (9.16) / Radius of dummy atoms: 1.90 A
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: TET12(1.10)S-f5b / Specimen concentration: 1.00-7.00 / Entity id: 811
BufferName: 20 mM Tris 150 mM NaCl 10% glycerol / pH: 7.5 / Sample id: 661
Entity #811Type: protein / Description: TET12(1.10)S-f5b / Formula weight: 53.191 / Number of molecules: 1 / Source: synthetic construct
Sequence:
MLEEELKQLE EELQAIEEQL AQLQWKAQAR KEKLAQLKEK LSGPGSPEDE NSQLEEKISQ LKQKNSQLKE EIQQLEYGSG PGDIEQELER AKQSIRRLEQ EVNQERSRMQ YLQTLLSKSG PGQLEDKVEE LLSKNYHLEN EVSRLKKLVG SGPGLEEELK QLEEELQAIE EQLAQLQWKA QARKEKLAQL KEKLSGPGSP EDEIQSLEEK NSQLKQEISQ LEEKNQQLKY GSGPGQLEDK VEELLSKNYH LENEVSRLKK LVGSGPGSPE DKNSQLKEEI QQLEEENQQL EEKISQLKYG SGPGSPEDEI QQLEEEISQL EQKNSQLKEK NQQLKYGSGP GSPEDKISQL KEENQQLEQK IQQLKEENSQ LEYGSGPGDI EQELERAKQS IRRLEQEVNQ ERSRMQYLQT LLSKSGPGSP EDKISQLKQK IQQLKQENQQ LEEENSQLEY GLEHHHHHHH H

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Experimental information

BeamInstrument name: ESRF BM29 / Instrument city: Grenoble / Instrument country: France / Type of source: X-ray synchrotronSynchrotron / Radiation wavelength: 0.1 / Dist spec to detc: 2.89
DetectorName: Pilatus 1M
Scan
Title: TET12(1.10)S-f5b / Detc id: 16 / Measurement date: Apr 7, 2016 / Storage temperature: 2 / Cell temperature: 2 / Exposure time: 2 / Number of frames: 10 / Unit: 1/nm / Qmin: 0.1694 / Qmax: 4.946 / Sample id: 661 / Result id: 702 / Beam id: 10
Distance distribution function P(R)
Intensity id: 702 / Software p of R: GNOM 5.0 / Number of points: 471 / Qmin: 0.197645 / Qmax: 2.41386 / P of R point min: 1 / P of R point max: 471 / Rmin: 0 / Rmax: 11.18
Result
SASBDB code: SASDCX7 / Experimental MW: 47.2 / MW standard: 47.2 / MW standard error: 0.2 / MW Porod: 75 / I0 from PR: 312.1 / I0 from PR error: 0.3 / I0 from Guinier: 312.65 / I0 from Guinier error: 1 / Rg from PR: 3.375 / Rg from PR error: 0.004 / Rg from Guinier: 3.38 / Rg from Guinier error: 0.02 / D max: 11.18 / Porod volume: 127 / Guinier point min: 7 / Guinier point max: 46 / Type of curve: single_conc / Dmax error: 1

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