+
Open data
-
Basic information
Entry | Database: SASBDB / ID: SASDAJ8 |
---|---|
![]() | Light state solution structure of Aureochrome1a- A´α-LOV-Jα
|
Biological species | ![]() ![]() |
![]() | ![]() Title: Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Authors: Ankan Banerjee / Elena Herman / Tilman Kottke / Lars-Oliver Essen / ![]() Abstract: Light-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of ...Light-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of photoreceptors that differs by its inversed topology with a C-terminal LOV sensor and an N-terminal effector (basic region leucine zipper, bZIP) domain. We crystallized the LOV domain including its flanking helices, A'α and Jα, of aureochrome 1a from Phaeodactylum tricornutum in the dark state and solved the structure at 2.8 Å resolution. Both flanking helices contribute to the interface of the native-like dimer. Small-angle X-ray scattering shows light-induced conformational changes limited to the dimeric envelope as well as increased flexibility in the lit state for the flanking helices. These rearrangements are considered to be crucial for the formation of the light-activated dimer. Finally, the LOV domain of the class 2 aureochrome PtAUREO2 was shown to lack a chromophore because of steric hindrance caused by M301. |
![]() |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-Models
Model #323 | ![]() Type: dummy / Software: DAMMIF / Radius of dummy atoms: 3.25 A Comment: The model is an averaged and volume corrected representation (DAMFILT) Chi-square value: 0.656 / P-value: 0.000005 ![]() |
---|
-
Sample
![]() | Name: Light state solution structure of Aureochrome1a- A´α-LOV-Jα Specimen concentration: 0.75-15.00 |
---|---|
Buffer | Name: Tris / Concentration: 10.00 mM / pH: 8 / Composition: 300 mM NaCl |
Entity #191 | Name: Aureochrome1a-A / Type: protein / Description: Aureochrome1a-A´α-LOV-Jα / Formula weight: 17.973 / Num. of mol.: 2 / Source: Phaeodactylum tricornutum Sequence: MGSSHHHHHH SSGLVPRGSH MDFSFIKALQ TAQQNFVVTD PSLPDNPIVY ASQGFLNLTG YSLDQILGRN CRFLQGPETD PKAVERIRKA IEQGNDMSVC LLNYRVDGTT FWNQFFIAAL RDAGGNVTNF VGVQCKVSDQ YAATVTKQQE EEEEAAANDD ED |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France ![]() | |||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Pilatus 1M | |||||||||||||||||||||||||||
Scan |
| |||||||||||||||||||||||||||
Distance distribution function P(R) |
| |||||||||||||||||||||||||||
Result | Comments: Solution scattering data of Aureochrome1a- A´α-LOV-Jα in the light state (blue light illuminated for 5-10 min). The measurement was performed at two concentration 42 and 833 micomolar ...Comments: Solution scattering data of Aureochrome1a- A´α-LOV-Jα in the light state (blue light illuminated for 5-10 min). The measurement was performed at two concentration 42 and 833 micomolar concentration. The buffer-only scattering was subtracted and the data sets were merged to generate representative curve. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
|