SASDBE2: Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

Basic information

• Entry: Database: SASBDB / ID: SASDBE2

Sample

Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

• Aureochrome1a- A´α-LOV-Jα (Light State) (protein), PtAUREO1a-LOV, Phaeodactylum tricornutum

• Function / homology: :
• Biological species: Phaeodactylum tricornutum (Diatom)
• Citation: Journal: Structure / Year: 2016; Title: Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.; Authors: Ankan Banerjee / Elena Herman / Tilman Kottke / Lars-Oliver Essen / ; Abstract: Light-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of photoreceptors that differs by its inversed topology with a C-terminal LOV sensor and an N-terminal effector (basic region leucine zipper, bZIP) domain. We crystallized the LOV domain including its flanking helices, A'α and Jα, of aureochrome 1a from Phaeodactylum tricornutum in the dark state and solved the structure at 2.8 Å resolution. Both flanking helices contribute to the interface of the native-like dimer. Small-angle X-ray scattering shows light-induced conformational changes limited to the dimeric envelope as well as increased flexibility in the lit state for the flanking helices. These rearrangements are considered to be crucial for the formation of the light-activated dimer. Finally, the LOV domain of the class 2 aureochrome PtAUREO2 was shown to lack a chromophore because of steric hindrance caused by M301.

Contact author

• Lars-Oliver Essen (Philipps-Universität Marburg, Marburg, Germany)

Models

• Model #396: ; Type: dummy / Software: DAMMIN / Radius of dummy atoms: 2.75 A / Chi-square value: 0.787; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα; Specimen concentration: 2.00-8.00
• Buffer: Name: Tris / Concentration: 10.00 mM / Composition: 300 mM NaCl

Entity #261

Name: PtAUREO1a-LOV / Type: protein / Description: Aureochrome1a- A´α-LOV-Jα (Light State) / Formula weight: 16.1 / Num. of mol.: 2 / Source: Phaeodactylum tricornutum / References: UniProt: JGI-49116
Sequence:

GSHMDFSFIK ALQTAQQNFV VTDPSLPDNP IVYASQGFLN LTGYSLDQIL GRNCRFLQGP ETDPKAVERI RKAIEQGNDM SVCLLNYRVD GTTFWNQFFI AALRDAGGNV TNFVGVQCKV SDQYAATVTK QQEEEEEAAA NDDED

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.93 Å / Dist. spec. to detc.: 2.43 mm
• Detector: Name: Pilatus 1M

Scan

Title: Aureochrome1a- A´α-LOV-Jα (Light State) / Measurement date: Sep 9, 2015 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 10 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.0364	4.9116

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 551 /

	Min	Max
Q	0.214219	2.7875
P(R) point	1	551
R	0	9.8

Result

D max: 9.8 / Type of curve: single_conc

	Experimental	Standard	Porod
MW	48.1 kDa	48.1 kDa	44.2 kDa
Volume	-	-	66.4 nm3

	P(R)	Guinier
Forward scattering, I0	48.35	48.1
Radius of gyration, Rg	2.65 nm	2.6 nm