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TitleStructure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.
Journal, issue, pagesStructure, Vol. 24, Issue 1, Page 171-178, Year 2016
Publish dateJan 5, 2016
AuthorsAnkan Banerjee / Elena Herman / Tilman Kottke / Lars-Oliver Essen /
PubMed AbstractLight-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of ...Light-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of photoreceptors that differs by its inversed topology with a C-terminal LOV sensor and an N-terminal effector (basic region leucine zipper, bZIP) domain. We crystallized the LOV domain including its flanking helices, A'α and Jα, of aureochrome 1a from Phaeodactylum tricornutum in the dark state and solved the structure at 2.8 Å resolution. Both flanking helices contribute to the interface of the native-like dimer. Small-angle X-ray scattering shows light-induced conformational changes limited to the dimeric envelope as well as increased flexibility in the lit state for the flanking helices. These rearrangements are considered to be crucial for the formation of the light-activated dimer. Finally, the LOV domain of the class 2 aureochrome PtAUREO2 was shown to lack a chromophore because of steric hindrance caused by M301.
External linksStructure / PubMed:26688213
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2.791 Å
Structure data

SASDAJ8:
Light state solution structure of Aureochrome1a- A´α-LOV-Jα
Method: SAXS/SANS

SASDAK8:
Dark state solution structure of Aureochrome1a- A´α-LOV-Jα
Method: SAXS/SANS

SASDBD2:
Dark state solution structure of untagged Aureochrome1a- A´α-LOV-Jα
Method: SAXS/SANS

SASDBE2:
Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα
Method: SAXS/SANS

PDB-5a8b:
Structure of a parallel dimer of the aureochrome 1a LOV domain from Phaeodactylum tricornutum
Method: X-RAY DIFFRACTION / Resolution: 2.791 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • phaeodactylum tricornutum (Diatom)
KeywordsUNKNOWN FUNCTION / AUREOCHROME 1 / PARALLEL LOV DIMER AND FLANKING HELIX.

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