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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7nvr | ||||||||||||||||||
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タイトル | Human Mediator with RNA Polymerase II Pre-initiation complex | ||||||||||||||||||
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![]() | TRANSCRIPTION / Mediator complex / RNA polymerase II / TFIIH / Pre-initiation complex | ||||||||||||||||||
機能・相同性 | ![]() negative regulation of smooth muscle cell differentiation / MMXD complex / core TFIIH complex portion of holo TFIIH complex / Cytosolic iron-sulfur cluster assembly / : / central nervous system myelin formation / positive regulation of core promoter binding / RNA polymerase II core complex assembly / positive regulation of mitotic recombination / hair cell differentiation ...negative regulation of smooth muscle cell differentiation / MMXD complex / core TFIIH complex portion of holo TFIIH complex / Cytosolic iron-sulfur cluster assembly / : / central nervous system myelin formation / positive regulation of core promoter binding / RNA polymerase II core complex assembly / positive regulation of mitotic recombination / hair cell differentiation / hair follicle maturation / ventricular system development / nucleotide-excision repair factor 3 complex / core mediator complex / transcription factor TFIIE complex / RNA polymerase transcription factor SL1 complex / nucleotide-excision repair, preincision complex assembly / meiotic sister chromatid cohesion / phosphatase activator activity / snRNA transcription by RNA polymerase II / CAK-ERCC2 complex / transcription factor TFIIK complex / RNA polymerase III general transcription initiation factor activity / TFIIF-class transcription factor complex binding / UV protection / transcriptional start site selection at RNA polymerase II promoter / RNA polymerase I core promoter sequence-specific DNA binding / RNA polymerase II CTD heptapeptide repeat Y1 kinase activity / RNA polymerase II CTD heptapeptide repeat S2 kinase activity / RNA polymerase II CTD heptapeptide repeat T4 kinase activity / RNA polymerase II CTD heptapeptide repeat S5 kinase activity / RNA polymerase II CTD heptapeptide repeat S7 kinase activity / transcription factor TFIIF complex / embryonic cleavage / DNA 5'-3' helicase / adult heart development / mediator complex / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Generic Transcription Pathway / transcription factor TFIIA complex / G protein-coupled receptor internalization / RNA Polymerase III Abortive And Retractive Initiation / female germ cell nucleus / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / cyclin-dependent protein serine/threonine kinase activator activity / male pronucleus / female pronucleus / germinal vesicle / RNA polymerase II general transcription initiation factor binding / nuclear vitamin D receptor binding / [RNA-polymerase]-subunit kinase / nuclear thyroid hormone receptor binding / limb development / regulation of cyclin-dependent protein serine/threonine kinase activity / Abortive elongation of HIV-1 transcript in the absence of Tat / cyclin-dependent protein serine/threonine kinase regulator activity / RNA Polymerase I Transcription Termination / FGFR2 alternative splicing / transcription preinitiation complex / regulation of mitotic cell cycle phase transition / Viral Messenger RNA Synthesis / DNA 3'-5' helicase / Signaling by FGFR2 IIIa TM / cortical actin cytoskeleton / 3'-5' DNA helicase activity / protein acetylation / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / cell division site / erythrocyte maturation / spinal cord development / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / hematopoietic stem cell proliferation / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.5 Å | ||||||||||||||||||
![]() | Rengachari, S. / Schilbach, S. / Aibara, S. / Cramer, P. | ||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structures of mammalian RNA polymerase II pre-initiation complexes. 著者: Shintaro Aibara / Sandra Schilbach / Patrick Cramer / ![]() 要旨: The initiation of transcription is a focal point for the regulation of gene activity during mammalian cell differentiation and development. To initiate transcription, RNA polymerase II (Pol II) ...The initiation of transcription is a focal point for the regulation of gene activity during mammalian cell differentiation and development. To initiate transcription, RNA polymerase II (Pol II) assembles with general transcription factors into a pre-initiation complex (PIC) that opens promoter DNA. Previous work provided the molecular architecture of the yeast and human PIC and a topological model for DNA opening by the general transcription factor TFIIH. Here we report the high-resolution cryo-electron microscopy structure of PIC comprising human general factors and Sus scrofa domesticus Pol II, which is 99.9% identical to human Pol II. We determine the structures of PIC with closed and opened promoter DNA at 2.5-2.8 Å resolution, and resolve the structure of TFIIH at 2.9-4.0 Å resolution. We capture the TFIIH translocase XPB in the pre- and post-translocation states, and show that XPB induces and propagates a DNA twist to initiate the opening of DNA approximately 30 base pairs downstream of the TATA box. We also provide evidence that DNA opening occurs in two steps and leads to the detachment of TFIIH from the core PIC, which may stop DNA twisting and enable RNA chain initiation. | ||||||||||||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 2.2 MB | 表示 | ![]() |
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PDB形式 | ![]() | 1.7 MB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.6 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.6 MB | 表示 | |
XML形式データ | ![]() | 255.9 KB | 表示 | |
CIF形式データ | ![]() | 423.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 12610MC ![]() 7nvsC ![]() 7nvtC ![]() 7nvuC ![]() 7nvvC ![]() 7nvwC ![]() 7nvxC ![]() 7nvyC ![]() 7nvzC ![]() 7nw0C M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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1 |
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要素
+タンパク質 , 8種, 8分子 0389DKLO
+General transcription ... , 9種, 9分子 124567QRW
+DNA-directed RNA polymerase ... , 7種, 7分子 ABCEFGI
+DNA-directed RNA polymerases I, II, and III subunit ... , 2種, 2分子 HJ
+Transcription initiation factor ... , 4種, 4分子 MUVX
+DNA鎖 , 2種, 2分子 NT
+Unassigned peptide, likely ... , 2種, 2分子 YZ
+Mediator of RNA polymerase II transcription subunit ... , 18種, 18分子 abcdefghijklmnopqs
+Unassigned peptide ... , 6種, 6分子 rvwxyz
+非ポリマー , 3種, 20分子 




+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 |
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分子量 | 実験値: NO | ||||||||||||||||||||||||||||||||||||||||||
由来(天然) |
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由来(組換発現) |
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緩衝液 | pH: 7.5 | ||||||||||||||||||||||||||||||||||||||||||
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||||||||||||||||||||
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 58.56 e/Å2 フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) |
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解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3次元再構成 | 解像度: 4.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 25967 / 対称性のタイプ: POINT |