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- EMDB-8305: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator ... -

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Basic information

Entry
Database: EMDB / ID: EMD-8305
TitleMediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail (Focused Refinement)
Map dataMed-Pic delta Med Tail after Relion 1.4 postprocessing
Sample
  • Complex: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail
    • Protein or peptide: x 47 types
    • DNA: x 1 types
Function / homology
Function and homology information


meiotic gene conversion / NuA3b histone acetyltransferase complex / NuA3a histone acetyltransferase complex / NuA3 histone acetyltransferase complex / regulation of mitotic recombination / RNA polymerase II promoter clearance / RNA polymerase II complex recruiting activity / TFIIA-class transcription factor complex binding / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly ...meiotic gene conversion / NuA3b histone acetyltransferase complex / NuA3a histone acetyltransferase complex / NuA3 histone acetyltransferase complex / regulation of mitotic recombination / RNA polymerase II promoter clearance / RNA polymerase II complex recruiting activity / TFIIA-class transcription factor complex binding / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / RNA polymerase III transcription regulatory region sequence-specific DNA binding / TFIIH-class transcription factor complex binding / core mediator complex / positive regulation of mitotic recombination / regulation of transcription by RNA polymerase III / nucleotide-excision repair factor 3 complex / RNA polymerase I general transcription initiation factor binding / transcription factor TFIIE complex / DNA translocase activity / nucleotide-excision repair, preincision complex assembly / negative regulation of ribosomal protein gene transcription by RNA polymerase II / transcription open complex formation at RNA polymerase II promoter / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / RPB4-RPB7 complex / transcription factor TFIIF complex / Ino80 complex / mediator complex / DNA 5'-3' helicase / transcription factor TFIIA complex / RNA polymerase I preinitiation complex assembly / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / SWI/SNF complex / DNA 3'-5' helicase / transcription preinitiation complex / DNA binding, bending / poly(A)+ mRNA export from nucleus / RNA Polymerase I Transcription Initiation / DNA duplex unwinding / Processing of Capped Intron-Containing Pre-mRNA / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / RNA Polymerase III Transcription Initiation From Type 2 Promoter / 3'-5' DNA helicase activity / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / TP53 Regulates Transcription of DNA Repair Genes / termination of RNA polymerase II transcription / transcription factor TFIID complex / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA polymerase II general transcription initiation factor activity / RNA Polymerase II Pre-transcription Events / RNA-templated transcription / termination of RNA polymerase III transcription / Formation of TC-NER Pre-Incision Complex / transcription initiation at RNA polymerase III promoter / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / termination of RNA polymerase I transcription / RNA Polymerase I Promoter Escape / RNA polymerase II complex binding / protein phosphatase activator activity / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription by RNA polymerase I / TFIID-class transcription factor complex binding / transcription initiation at RNA polymerase I promoter / nuclear-transcribed mRNA catabolic process / ATPase activator activity / Estrogen-dependent gene expression / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / transcription by RNA polymerase III / RNA polymerase II activity / Dual incision in TC-NER / positive regulation of transcription initiation by RNA polymerase II / transcription elongation by RNA polymerase I / RNA polymerase II core promoter sequence-specific DNA binding / transcription-coupled nucleotide-excision repair / tRNA transcription by RNA polymerase III / RNA polymerase I complex / RNA polymerase I activity / RNA polymerase III complex / translesion synthesis / positive regulation of translational initiation / cellular response to nutrient levels / ATP-dependent activity, acting on DNA / RNA polymerase II, core complex / RNA polymerase II preinitiation complex assembly / translation initiation factor binding / DNA helicase activity / TBP-class protein binding / isomerase activity / DNA-templated transcription initiation / nucleotide-excision repair / transcription initiation at RNA polymerase II promoter / positive regulation of transcription elongation by RNA polymerase II
Similarity search - Function
SAS complex subunit SAS5/transcription initiation factor TFIID subunit 14 / Mediator of RNA polymerase II transcription subunit 22, Saccharomycetes / Mediator of RNA polymerase II transcription subunit 20 / Mediator complex, subunit Med6, fungi / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Tfa2 E-tether / Mediator complex, subunit Med31 ...SAS complex subunit SAS5/transcription initiation factor TFIID subunit 14 / Mediator of RNA polymerase II transcription subunit 22, Saccharomycetes / Mediator of RNA polymerase II transcription subunit 20 / Mediator complex, subunit Med6, fungi / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Tfa2 E-tether / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / MED7 protein / Mediator complex, subunit Med20 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Bacterial type XPD DNA helicase, FeS cluster domain / Med18 protein / YEATS / YEATS superfamily / YEATS family / YEATS domain profile. / RAD3/XPD family / Helicase XPB/Ssl2 / ERCC3/RAD25/XPB helicase, C-terminal domain / Helicase XPB/Ssl2, N-terminal domain / Helicase conserved C-terminal domain / ERCC3/RAD25/XPB C-terminal helicase / Helical and beta-bridge domain / Helical and beta-bridge domain / Transcription factor TFIIH subunit p52/Tfb2 / Transcription factor Tfb2, C-terminal domain / Transcription factor Tfb2 / Transcription factor Tfb2 (p52) C-terminal domain / Transcription factor TFIIE beta subunit, DNA-binding domain / TFIIH subunit TTDA/Tfb5 / Transcription initiation factor TFIIE, beta subunit / TFB5-like superfamily / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / Transcription factor TFIIH complex subunit Tfb5 / TFA2 Winged helix domain 2 / TFIIE beta central core DNA-binding domain profile. / Transcription factor TFIIH complex subunit Tfb5 / ATP-dependent helicase Rad3/Chl1-like / : / Helicase-like, DEXD box c2 type / DEAD2 / DEAD_2 / DEXDc2 / Helicase superfamily 1/2, DinG/Rad3-like / HELICc2 / ATP-dependent helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-type / Helicase C-terminal domain / Superfamilies 1 and 2 helicase ATP-binding type-2 domain profile. / Transcription initiation factor IIE subunit alpha, N-terminal / Transcription factor TFE/TFIIEalpha HTH domain / TFIIEalpha/SarR/Rpc3 HTH domain / Transcription factor E / TFIIE alpha subunit / TFE/IIEalpha-type HTH domain profile. / Transcription initiation factor IIE / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription initiation factor IIA, gamma subunit / Transcription factor IIA, alpha-helical domain / Transcription factor IIA, beta-barrel / Transcription initiation factor IIA, gamma subunit, C-terminal / Transcription initiation factor IIA, gamma subunit, N-terminal / Transcription initiation factor IIA, gamma subunit, helical domain / Transcription initiation factor IIA, gamma subunit / Transcription initiation factor IIF, beta subunit / TFIIF beta subunit, HTH domain / TFIIF, beta subunit, N-terminal / TFIIF, beta subunit HTH domain / TFIIF, beta subunit N-terminus / Transcription initiation factor IIF, alpha subunit / Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) / Transcription Factor IIF, Rap30/Rap74, interaction / Transcription factor TFIIB, cyclin-like domain / Transcription factor TFIIB, conserved site / Transcription factor TFIIB repeat
Similarity search - Domain/homology
DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 ...DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / Transcription initiation factor IIA large subunit / Transcription initiation factor IIA subunit 2 / Mediator of RNA polymerase II transcription subunit 9 / DNA-directed RNA polymerase II subunit RPB7 / Mediator of RNA polymerase II transcription subunit 20 / Transcription initiation factor TFIID subunit 14 / Transcription initiation factor IIE subunit alpha / Transcription initiation factor IIE subunit beta / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 6 / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / Mediator of RNA polymerase II transcription subunit 21 / General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 / General transcription and DNA repair factor IIH subunit TFB2 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 4 / General transcription and DNA repair factor IIH subunit TFB5 / Mediator of RNA polymerase II transcription subunit 11
Similarity search - Component
Biological speciesyeast (yeast) / Saccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 15.3 Å
AuthorsRobinson PJ / Bushnell DA / Kornberg RD
Funding support United States, 5 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI21144 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM36659 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM49985 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM103481 United States
National Science Foundation (NSF, United States)1231306 United States
CitationJournal: Cell / Year: 2016
Title: Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
Authors: Philip J Robinson / Michael J Trnka / David A Bushnell / Ralph E Davis / Pierre-Jean Mattei / Alma L Burlingame / Roger D Kornberg /
Abstract: A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass ...A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
History
DepositionAug 5, 2016-
Header (metadata) releaseSep 14, 2016-
Map releaseSep 14, 2016-
UpdateFeb 3, 2021-
Current statusFeb 3, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.025
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  • Surface view with fitted model
  • Atomic models: PDB-5sva
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-5sva
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8305.png

Downloads & links

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Map

FileDownload / File: emd_8305.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMed-Pic delta Med Tail after Relion 1.4 postprocessing
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.29 Å/pix.
x 220 pix.
= 503.8 Å		2.29 Å/pix.
x 220 pix.
= 503.8 Å		2.29 Å/pix.
x 220 pix.
= 503.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.29 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum-0.044287015 - 0.15345626
Average (Standard dev.)0.0015833485 (±0.010356013)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 503.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.292.292.29
M x/y/z220220220
origin x/y/z0.0000.0000.000
length x/y/z503.800503.800503.800
α/β/γ90.00090.00090.000
start NX/NY/NZ-210-210-210
NX/NY/NZ420420420
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS220220220
D min/max/mean-0.0440.1530.002

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Supplemental data

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Mask #1

Fileemd_8305_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Even Half map from Relion Auto3D, input to postprocessing

Fileemd_8305_half_map_1.map
AnnotationEven Half map from Relion Auto3D, input to postprocessing
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Odd Half map from Relion Auto3D, input to postprocessing

Fileemd_8305_half_map_2.map
AnnotationOdd Half map from Relion Auto3D, input to postprocessing
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail

EntireName: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail
Components
  • Complex: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB1
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB4
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC 1
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC 2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC 5
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB11
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC 4
    • Protein or peptide: Transcription initiation factor IIB
    • Protein or peptide: TATA-box-binding protein
    • Protein or peptide: Transcription initiation factor IIF subunit alpha
    • Protein or peptide: Transcription initiation factor IIF subunit beta
    • Protein or peptide: Transcription initiation factor IIF subunit gamma
    • Protein or peptide: Transcription factor TFIIE subunit TFA1
    • Protein or peptide: Transcription factor TFIIE subunit TFA2
    • Protein or peptide: Transcription initiation factor IIA large subunit
    • Protein or peptide: Transcription initiation factor IIA subunit 2
    • Protein or peptide: RNA polymerase II transcription factor B subunit 1
    • Protein or peptide: RNA polymerase II transcription factor B subunit 2
    • Protein or peptide: RNA polymerase II transcription factor B subunit 3
    • Protein or peptide: RNA polymerase II transcription factor B subunit 4
    • Protein or peptide: RNA polymerase II transcription factor B subunit 5
    • Protein or peptide: Serine/threonine-protein kinase KIN28
    • Protein or peptide: Cyclin CCL1
    • Protein or peptide: DNA repair helicase RAD3
    • Protein or peptide: Suppressor of stem-loop protein 1
    • Protein or peptide: DNA repair helicase RAD25
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
    • DNA: 108 bp HIS4 promoter fragment

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Supramolecule #1: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail

SupramoleculeName: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Molecular weightTheoretical: 1.99 MDa

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Macromolecule #1: DNA-directed RNA polymerase II subunit RPB1

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB1 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKPV FHVGFIAKIK KVCECVCMHC GKLLLDEHNE LMRQALAIKD SKKRFAAIWT LCKTKMVCET DVPSEDDPTQ LVSRGGCGNT ...String:
MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKPV FHVGFIAKIK KVCECVCMHC GKLLLDEHNE LMRQALAIKD SKKRFAAIWT LCKTKMVCET DVPSEDDPTQ LVSRGGCGNT QPTIRKDGLK LVGSWKKDRA TGDADEPELR VLSTEEILNI FKHISVKDFT SLGFNEVFSR PEWMILTCLP VPPPPVRPSI SFNESQRGED DLTFKLADIL KANISLETLE HNGAPHHAIE EAESLLQFHV ATYMDNDIAG QPQALQKSGR PVKSIRARLK GKEGRIRGNL MGKRVDFSAR TVISGDPNLE LDQVGVPKSI AKTLTYPEVV TPYNIDRLTQ LVRNGPNEHP GAKYVIRDSG DRIDLRYSKR AGDIQLQYGW KVERHIMDND PVLFNRQPSL HKMSMMAHRV KVIPYSTFRL NLSVTSPYNA DFDGDEMNLH VPQSEETRAE LSQLCAVPLQ IVSPQSNKPC MGIVQDTLCG IRKLTLRDTF IELDQVLNML YWVPDWDGVI PTPAIIKPKP LWSGKQILSV AIPNGIHLQR FDEGTTLLSP KDNGMLIIDG QIIFGVVEKK TVGSSNGGLI HVVTREKGPQ VCAKLFGNIQ KVVNFWLLHN GFSTGIGDTI ADGPTMREIT ETIAEAKKKV LDVTKEAQAN LLTAKHGMTL RESFEDNVVR FLNEARDKAG RLAEVNLKDL NNVKQMVMAG SKGSFINIAQ MSACVGQQSV EGKRIAFGFV DRTLPHFSKD DYSPESKGFV ENSYLRGLTP QEFFFHAMGG REGLIDTAVK TAETGYIQRR LVKALEDIMV HYDNTTRNSL GNVIQFIYGE DGMDAAHIEK QSLDTIGGSD AAFEKRYRVD LLNTDHTLDP SLLESGSEIL GDLKLQVLLD EEYKQLVKDR KFLREVFVDG EANWPLPVNI RRIIQNAQQT FHIDHTKPSD LTIKDIVLGV KDLQENLLVL RGKNEIIQNA QRDAVTLFCC LLRSRLATRR VLQEYRLTKQ AFDWVLSNIE AQFLRSVVHP GEMVGVLAAQ SIGEPATQMT LNTFHFAGVA SKKVTSGVPR LKEILNVAKN MKTPSLTVYL EPGHAADQEQ AKLIRSAIEH TTLKSVTIAS EIYYDPDPRS TVIPEDEEII QLHFSLLDEE AEQSFDQQSP WLLRLELDRA AMNDKDLTMG QVGERIKQTF KNDLFVIWSE DNDEKLIIRC RVVRPKSLDA ETEAEEDHML KKIENTMLEN ITLRGVENIE RVVMMKYDRK VPSPTGEYVK EPEWVLETDG VNLSEVMTVP GIDPTRIYTN SFIDIMEVLG IEAGRAALYK EVYNVIASDG SYVNYRHMAL LVDVMTTQGG LTSVTRHGFN RSNTGALMRC SFEETVEILF EAGASAELDD CRGVSENVIL GQMAPIGTGA FDVMIDEESL VKYMPEQKIT EIEDGQDGGV TPYSNESGLV NADLDVKDEL MFSPLVDSGS NDAMAGGFTA YGGADYGEAT SPFGAYGEAP TSPGFGVSSP GFSPTSPTYS PTSPAYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPA YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPGY SPGSPAYSPK QDEQKHNENE NSR

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Macromolecule #2: DNA-directed RNA polymerase II subunit RPB2

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB2 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP MVNESDGVTH ALYPQEARLR NLTYSSGLFV DVKKRTYEAI DVPGRELKYE LIAEESEDDS ESGKVFIGRL ...String:
MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP MVNESDGVTH ALYPQEARLR NLTYSSGLFV DVKKRTYEAI DVPGRELKYE LIAEESEDDS ESGKVFIGRL PIMLRSKNCY LSEATESDLY KLKECPFDMG GYFIINGSEK VLIAQERSAG NIVQVFKKAA PSPISHVAEI RSALEKGSRF ISTLQVKLYG REGSSARTIK ATLPYIKQDI PIVIIFRALG IIPDGEILEH ICYDVNDWQM LEMLKPCVED GFVIQDRETA LDFIGRRGTA LGIKKEKRIQ YAKDILQKEF LPHITQLEGF ESRKAFFLGY MINRLLLCAL DRKDQDDRDH FGKKRLDLAG PLLAQLFKTL FKKLTKDIFR YMQRTVEEAH DFNMKLAINA KTITSGLKYA LATGNWGEQK KAMSSRAGVS QVLNRYTYSS TLSHLRRTNT PIGRDGKLAK PRQLHNTHWG LVCPAETPEG QACGLVKNLS LMSCISVGTD PMPIITFLSE WGMEPLEDYV PHQSPDATRV FVNGVWHGVH RNPARLMETL RTLRRKGDIN PEVSMIRDIR EKELKIFTDA GRVYRPLFIV EDDESLGHKE LKVRKGHIAK LMATEYQDIE GGFEDVEEYT WSSLLNEGLV EYIDAEEEES ILIAMQPEDL EPAEANEEND LDVDPAKRIR VSHHATTFTH CEIHPSMILG VAASIIPFPD HNQSPRNTYQ SAMGKQAMGV FLTNYNVRMD TMANILYYPQ KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGTY DKLDDDGLIA PGVRVSGEDV IIGKTTPISP DEEELGQRTA YHSKRDASTP LRSTENGIVD QVLVTTNQDG LKFVKVRVRT TKIPQIGDKF ASRHGQKGTI GITYRREDMP FTAEGIVPDL IINPHAIPSR MTVAHLIECL LSKVAALSGN EGDASPFTDI TVEGISKLLR EHGYQSRGFE VMYNGHTGKK LMAQIFFGPT YYQRLRHMVD DKIHARARGP MQVLTRQPVE GRSRDGGLRF GEMERDCMIA HGAASFLKER LMEASDAFRV HICGICGLMT VIAKLNHNQF ECKGCDNKID IYQIHIPYAA KLLFQELMAM NITPRLYTDR SRDF

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Macromolecule #3: DNA-directed RNA polymerase II subunit RPB3

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCED HCDKCSVVLT LQAFGESEST TNVYSKDLVI VSNLMGRNIG HPIIQDKEGN GVLICKLRKG QELKLTCVAK KGIAKEHAKW ...String:
MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCED HCDKCSVVLT LQAFGESEST TNVYSKDLVI VSNLMGRNIG HPIIQDKEGN GVLICKLRKG QELKLTCVAK KGIAKEHAKW GPAAAIEFEY DPWNKLKHTD YWYEQDSAKE WPQSKNCEYE DPPNEGDPFD YKAQADTFYM NVESVGSIPV DQVVVRGIDT LQKKVASILL ALTQMDQDKV NFASGDNNTA SNMLGSNEDV MMTGAEQDPY SNASQMGNTG SGGYDNAW

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Macromolecule #4: DNA-directed RNA polymerase II subunit RPB4

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB4 / type: protein_or_peptide / ID: 4 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENANDE TTAVEDEDDD LDEDDVNADD DDFMHSETRE KELESIDVLL EQTTGGNNKD LKNTMQYLTN FSRFRDQETV GAVIQLLKST ...String:
MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENANDE TTAVEDEDDD LDEDDVNADD DDFMHSETRE KELESIDVLL EQTTGGNNKD LKNTMQYLTN FSRFRDQETV GAVIQLLKST GLHPFEVAQL GSLACDTADE AKTLIPSLNN KISDDELERI LKELSNLETL Y

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Macromolecule #5: DNA-directed RNA polymerases I, II, and III subunit RPABC 1

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC 1
type: protein_or_peptide / ID: 5 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVGV KTMKTFVIHI QEKNFQTGIF VYQNNITPSA MKLVPSIPPA TIETFNEAAL VVNITHHELV PKHIRLSSDE KRELLKRYRL ...String:
MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVGV KTMKTFVIHI QEKNFQTGIF VYQNNITPSA MKLVPSIPPA TIETFNEAAL VVNITHHELV PKHIRLSSDE KRELLKRYRL KESQLPRIQR ADPVALYLGL KRGEVVKIIR KSETSGRYAS YRICM

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Macromolecule #6: DNA-directed RNA polymerases I, II, and III subunit RPABC 2

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC 2
type: protein_or_peptide / ID: 6 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MSDYEEAFND GNENFEDFDV EHFSDEETYE EKPQFKDGET TDANGKTIVT GGNGPEDFQQ HEQIRRKTLK EKAIPKDQRA TTPYMTKYER ARILGTRALQ ISMNAPVFVD LEGETDPLRI AMKELAEKKI PLVIRRYLPD GSFEDWSVEE LIVDL

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Macromolecule #7: DNA-directed RNA polymerase II subunit RPB7

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 7 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD YDNIDIQRGR ILPTDGSAEF NVKYRAVVFK PFKGEVVDGT VVSCSQHGFE VQVGPMKVFV TKHLMPQDLT FNAGSNPPSY QSSEDVITIK SRIRVKIEGC ISQVSSIHAI GSIKEDYLGA I

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Macromolecule #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC3
type: protein_or_peptide / ID: 8 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSLA DDYDYVMYGT AYKFEEVSKD LIAVYYSFGG LLMRLEGNYR NLNNLKQENA YLLIRR

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Macromolecule #9: DNA-directed RNA polymerase II subunit RPB9

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 9 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MTTFRFCRDC NNMLYPREDK ENNRLLFECR TCSYVEEAGS PLVYRHELIT NIGETAGVVQ DIGSDPTLPR SDRECPKCHS RENVFFQSQQ RRKDTSMVLF FVCLSCSHIF TSDQKNKRTQ FS

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Macromolecule #10: DNA-directed RNA polymerases I, II, and III subunit RPABC 5

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC 5
type: protein_or_peptide / ID: 10 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MIVPVRCFSC GKVVGDKWES YLNLLQEDEL DEGTALSRLG LKRYCCRRMI LTHVDLIEKF LRYNPLEKRD

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Macromolecule #11: DNA-directed RNA polymerase II subunit RPB11

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB11 / type: protein_or_peptide / ID: 11 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MNAPDRFELF LLGEGESKLK IDPDTKAPNA VVITFEKEDH TLGNLIRAEL LNDRKVLFAA YKVEHPFFAR FKLRIQTTEG YDPKDALKNA CNSIINKLGA LKTNFETEWN LQTLAADDAF

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Macromolecule #12: DNA-directed RNA polymerases I, II, and III subunit RPABC 4

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC 4
type: protein_or_peptide / ID: 12 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MSREGFQIPT NLDAAAAGTS QARTATLKYI CAECSSKLSL SRTDAVRCKD CGHRILLKAR TKRLVQFEAR

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Macromolecule #13: Transcription initiation factor IIB

MacromoleculeName: Transcription initiation factor IIB / type: protein_or_peptide / ID: 13 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES ...String:
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK

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Macromolecule #14: TATA-box-binding protein

MacromoleculeName: TATA-box-binding protein / type: protein_or_peptide / ID: 14 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI ...String:
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM

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Macromolecule #15: Transcription initiation factor IIF subunit alpha

MacromoleculeName: Transcription initiation factor IIF subunit alpha / type: protein_or_peptide / ID: 15 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEE REKMLLQIGV EADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL K FQSKKKIN PVTDFHLPVR LHRKDTRNLQ FQLTRAEIVQ RQKEISEYKK ...String:
MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEE REKMLLQIGV EADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL K FQSKKKIN PVTDFHLPVR LHRKDTRNLQ FQLTRAEIVQ RQKEISEYKK KAEQERSTPN SG GMNKSGT VSLNNTVKDG SQTPTVDSVT KDNTANGVNS SIPTVTGSSV PPASPTTVSA VES NGLSNG STSAANGLDG NASTANLANG RPLVTKLEDA GPAEDPTKVG MVKYDGKEVT NEPE FEEGT MDPLADVAPD GGGRAKRGNL RRKTRQLKVL DENAKKLRFE EFYPWVMEDF DGYNT WVGS YEAGNSDSYV LLSVEDDGSF TMIPADKVYK FTARNKYATL TIDEAEKRMD KKSGEV PRW LMKHLDNIGT TTTRYDRTRR KLKAVADQQA MDEDDRDDNS EVELDYDEEF ADDEEAP II DGNEQENKES EQRIKKEMLQ ANAMGLRDEE APSENEEDEL FGEKKIDEDG ERIKKALQ K TELAALYSSD ENEINPYLSE SDIENKENES PVKKEEDSDT LSKSKRSSPK KQQKKATNA HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS NTVPSPIKQE EGLNSTVAE REETPAPTIT EKDIIEAIGD GKVNIKEFGK FIRRKYPGAE NKKLMFAIVK K LCRKVGND HMELKKE

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Macromolecule #16: Transcription initiation factor IIF subunit beta

MacromoleculeName: Transcription initiation factor IIF subunit beta / type: protein_or_peptide / ID: 16 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE ...String:
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE EELKKKQQQQ KRRNNRKKFN HRVMTDRDGR DRYIPYVKTI PKKTAIVGTV CHECQVMPSM NDPNYHKIVE QR RNIVKLN NKERITTLDE TVGVTMSHTG MSMRSDNSNF LKVGREKAKS NIKSIRMPKK EILDYLFKLF DEYDYWSLKG LKE RTRQPE AHLKECLDKV ATLVKKGPYA FKYTLRPEYK KLKEEERKAT LGELADEQTG SAGDNAQGDA EADLEDEIEM EDVV

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Macromolecule #17: Transcription initiation factor IIF subunit gamma

MacromoleculeName: Transcription initiation factor IIF subunit gamma / type: protein_or_peptide / ID: 17 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI FDKVIYHLHP TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK IPHDLNFLQE SYEVEHVIQI PLNKPLLTEE LAKSGSTEET TANTGTIGKR RTTTNTTAEP KAKRAKTGSA ...String:
MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI FDKVIYHLHP TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK IPHDLNFLQE SYEVEHVIQI PLNKPLLTEE LAKSGSTEET TANTGTIGKR RTTTNTTAEP KAKRAKTGSA STVKGSVDLE KLAFGLTKLN EDDLVGVVQM VTDNKTPEMN VTNNVEEGEF IIDLYSLPEG LLKSLWDYVK KNTE

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Macromolecule #18: Transcription factor TFIIE subunit TFA1

MacromoleculeName: Transcription factor TFIIE subunit TFA1 / type: protein_or_peptide / ID: 18 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW KVHQVVQRLK DDLDKNSEPN GYMCPICLTK YTQLEAVQLL NFDRTEFLCS LCDEPLVEDD SGKKNKEKQD ...String:
MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW KVHQVVQRLK DDLDKNSEPN GYMCPICLTK YTQLEAVQLL NFDRTEFLCS LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ PIIDSLKKID DSRIEENTFE IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM GTALGNDSSR RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE RENEKTLNDY YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN DETARENALE DEFEDVTDTA GTAKTESNTS NDVKQESIND KTEDAVNATA TASGPSANAK PNDGDDDDDD DDDEMDIEFE DV

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Macromolecule #19: Transcription factor TFIIE subunit TFA2

MacromoleculeName: Transcription factor TFIIE subunit TFA2 / type: protein_or_peptide / ID: 19 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL NSAKNPVLVD IKKEAGSNSS NAISLDDDDD DEDFGSSPSK KVRPGSIAAA ALQANQTDIS KSHDSSKLLW ATEYIQKKGK PVLVNELLDY LSMKKDDKVI ELLKKLDRIE ...String:
MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL NSAKNPVLVD IKKEAGSNSS NAISLDDDDD DEDFGSSPSK KVRPGSIAAA ALQANQTDIS KSHDSSKLLW ATEYIQKKGK PVLVNELLDY LSMKKDDKVI ELLKKLDRIE FDPKKGTFKY LSTYDVHSPS ELLKLLRSQV TFKGISCKDL KDGWPQCDET INQLEEDSKI LVLRTKKDKT PRYVWYNSGG NLKCIDEEFV KMWENVQLPQ FAELPRKLQD LGLKPASVDP ATIKRQTKRV EVKKKRQRKG KITNTHMTGI LKDYSHRV

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Macromolecule #20: Transcription initiation factor IIA large subunit

MacromoleculeName: Transcription initiation factor IIA large subunit / type: protein_or_peptide / ID: 20 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI EVKPEIELTI NNANITTVEN ...String:
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI EVKPEIELTI NNANITTVEN IDDESEKKDD EEKEEDVEKT RKEKEQIEQV KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD ENLMLCLYDK VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV

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Macromolecule #21: Transcription initiation factor IIA subunit 2

MacromoleculeName: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 21 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG DSQSVISVDK LRIVACNSKK SE

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Macromolecule #22: RNA polymerase II transcription factor B subunit 1

MacromoleculeName: RNA polymerase II transcription factor B subunit 1 / type: protein_or_peptide / ID: 22 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADIYEEK RRREESAQHT ETPMSSSSVT AGTPTPHLDT PQLNNGAPLI NTAKLDDSLS ...String:
MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADIYEEK RRREESAQHT ETPMSSSSVT AGTPTPHLDT PQLNNGAPLI NTAKLDDSLS KEKLLTNLKL QQSLLKGNKV LMKVFQETVI NAGLPPSEFW STRIPLLRAF ALSTSQKVGP YNVLSTIKPV ASSENKVNVN LSREKILNIF ENYPIVKKAY TDNVPKNFKE PEFWARFFSS KLFRKLRGEK IMQNDRGDVI IDRYLTLDQE FDRKDDDMLL HPVKKIIDLD GNIQDDPVVR GNRPDFTMQP GVDINGNSDG TVDILKGMNR LSEKMIMALK NEYSRTNLQN KSNITNDEED EDNDERNELK IDDLNESYKT NYAIIHLKRN AHEKTTDNDA KSSADSIKNA DLKVSNQQML QQLSLVMDNL INKLDLNQVV PNNEVSNKIN KRVITAIKIN AKQAKHNNVN SALGSFVDNT SQANELEVKS TLPIDLLESC RMLHTTCCEF LKHFYIHFQS GEQKQASTVK KLYNHLKDCI EKLNELFQDV LNGDGESMSN TCTAYLKPVL NSITLATHKY DEYFNEYNNN SN

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Macromolecule #23: RNA polymerase II transcription factor B subunit 2

MacromoleculeName: RNA polymerase II transcription factor B subunit 2 / type: protein_or_peptide / ID: 23 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIPN KSSGTLMINL NPTFKISLRN ALTGGEVQNS FGVVVEENVV SLDLLDEYSA NKWETILHFM VGTPLAKIPS EKVLNLLKHS ...String:
MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIPN KSSGTLMINL NPTFKISLRN ALTGGEVQNS FGVVVEENVV SLDLLDEYSA NKWETILHFM VGTPLAKIPS EKVLNLLKHS KLMEEVNSTG EFKITNEGFQ FLLQEINSQL WTLLLQYLKM IETSKMDLVD VLHFIFMLGA LEVGKAYKID ALSETQRIML QDMRDYGLVF QKHSNDSIFY PTKLALMLTS DTKTIRSASN AMDSVLRQNR EEPSVNEDGA NGKSTTDITT SDDLNKAGLK NQDIPDGSLI VETNFKIYSY SNSPLQIAVL SLFVHLKARF VNMVLGQITR ESIRRALTNG ITADQIIAYL ETHAHPQMRR LAEEKLEKKL ELDPNCKEPL QVLPPTVVDQ IRLWQLELDR VITYEGSLYS DFETSQEYNL LSKYAQDIGV LLWKDDKKKK FFISKEGNSQ VLDFAKRKLK KKQ

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Macromolecule #24: RNA polymerase II transcription factor B subunit 3

MacromoleculeName: RNA polymerase II transcription factor B subunit 3 / type: protein_or_peptide / ID: 24 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER SRTEIESFEQ RQKFEKEMKL ...String:
MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER SRTEIESFEQ RQKFEKEMKL KKRLLERQIE EEERMNKEWT KKEIVNRLST TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF NSNVNVQNSR LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY AYERVLTEAF MGLGCVISEE L

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Macromolecule #25: RNA polymerase II transcription factor B subunit 4

MacromoleculeName: RNA polymerase II transcription factor B subunit 4 / type: protein_or_peptide / ID: 25 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MDAISDPTFK HARSRKQVTE ESPSLLTVII EIAPKLWTTF DEEGNEKGSI IKVLEALIVF LNAHLAFNSA NKVAVIAAYS QGIKYLYPE STSALKASES ENKTRSDLKI INSDMYRRFR NVDETLVEEI YKLFELEKKQ IEQNSQRSTL AGAMSAGLTY V NRISKESV ...String:
MDAISDPTFK HARSRKQVTE ESPSLLTVII EIAPKLWTTF DEEGNEKGSI IKVLEALIVF LNAHLAFNSA NKVAVIAAYS QGIKYLYPE STSALKASES ENKTRSDLKI INSDMYRRFR NVDETLVEEI YKLFELEKKQ IEQNSQRSTL AGAMSAGLTY V NRISKESV TTSLKSRLLV LTCGSGSSKD EIFQYIPIMN CIFSATKMKC PIDVVKIGGS KESTFLQQTT DATNGVYLHV ES TEGLIQY LATAMFIDPS LRPIIVKPNH GSVDFRTSCY LTGRVVAVGF ICSVCLCVLS IIPPGNKCPA CDSQFDEHVI AKL KRKPVV PRLKAKKKVT KP

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Macromolecule #26: RNA polymerase II transcription factor B subunit 5

MacromoleculeName: RNA polymerase II transcription factor B subunit 5 / type: protein_or_peptide / ID: 26 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MARARKGALV QCDPSIKALI LQIDAKMSDI VLEELDDTHL LVNPSKVEFV KHELNRLLSK NIYNPMDEEE NQ

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Macromolecule #27: Serine/threonine-protein kinase KIN28

MacromoleculeName: Serine/threonine-protein kinase KIN28 / type: protein_or_peptide / ID: 27 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY ...String:
MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY RAPELLFGAK HYTSAIDIWS VGVIFAELML RIPYLPGQND VDQMEVTFRA LGTPTDRDWP EVSSFMTYNK LQIYPPPSRD ELRKRFIAAS EYALDFMCGM LTMNPQKRWT AVQCLESDYF KELPPPSDPS SIKIRN

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Macromolecule #28: Cyclin CCL1

MacromoleculeName: Cyclin CCL1 / type: protein_or_peptide / ID: 28 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MTDIQLNGKS TLDTPSATMS AKEKEAKLKS ADENNKPPNY KRISDDDLYR HSSQYRMWSY TKDQLQEKRV DTNARAIAYI EENLLKFREA HNLTEEEIKV LEAKAIPLTM EEELDLVNFY AKKVQVIAQH LNLPTEVVAT AISFFRRFFL ENSVMQIDPK SIVHTTIFLA ...String:
MTDIQLNGKS TLDTPSATMS AKEKEAKLKS ADENNKPPNY KRISDDDLYR HSSQYRMWSY TKDQLQEKRV DTNARAIAYI EENLLKFREA HNLTEEEIKV LEAKAIPLTM EEELDLVNFY AKKVQVIAQH LNLPTEVVAT AISFFRRFFL ENSVMQIDPK SIVHTTIFLA CKSENYFISV DSFAQKAKST RDSVLKFEFK LLESLKFSLL NHHPYKPLHG FFLDIQNVLY GKVDLNYMGQ IYDRCKKRIT AALLTDVVYF YTPPQITLAT LLIEDEALVT RYLETKFPSR EGSQESVPGN EKEEPQNDAS TTEKNKEKST ESEEYSIDSA KLLTIIRECK SIIEDCKPPS TEEAKKIAAK NYYCQNPSTL IQKLKRKLNG EDTSSTVEKK QKT

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Macromolecule #29: DNA repair helicase RAD3

MacromoleculeName: DNA repair helicase RAD3 / type: protein_or_peptide / ID: 29 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ MHYPEHRKII YCSRTMSEIE KALVELENLM DYRTKELGYQ EDFRGLGLTS RKNLCLHPEV SKERKGTVVD EKCRRMTNGQ AKRKLEEDPE ANVELCEYHE NLYNIEVEDY ...String:
MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ MHYPEHRKII YCSRTMSEIE KALVELENLM DYRTKELGYQ EDFRGLGLTS RKNLCLHPEV SKERKGTVVD EKCRRMTNGQ AKRKLEEDPE ANVELCEYHE NLYNIEVEDY LPKGVFSFEK LLKYCEEKTL CPYFIVRRMI SLCNIIIYSY HYLLDPKIAE RVSNEVSKDS IVIFDEAHNI DNVCIESLSL DLTTDALRRA TRGANALDER ISEVRKVDSQ KLQDEYEKLV QGLHSADILT DQEEPFVETP VLPQDLLTEA IPGNIRRAEH FVSFLKRLIE YLKTRMKVLH VISETPKSFL QHLKQLTFIE RKPLRFCSER LSLLVRTLEV TEVEDFTALK DIATFATLIS TYEEGFLLII EPYEIENAAV PNPIMRFTCL DASIAIKPVF ERFSSVIITS GTISPLDMYP RMLNFKTVLQ KSYAMTLAKK SFLPMIITKG SDQVAISSRF EIRNDPSIVR NYGSMLVEFA KITPDGMVVF FPSYLYMESI VSMWQTMGIL DEVWKHKLIL VETPDAQETS LALETYRKAC SNGRGAILLS VARGKVSEGI DFDHQYGRTV LMIGIPFQYT ESRILKARLE FMRENYRIRE NDFLSFDAMR HAAQCLGRVL RGKDDYGVMV LADRRFSRKR SQLPKWIAQG LSDADLNLST DMAISNTKQF LRTMAQPTDP KDQEGVSVWS YEDLIKHQNS RKDQGGFIEN ENKEGEQDED EDEDIEMQ

+
Macromolecule #30: Suppressor of stem-loop protein 1

MacromoleculeName: Suppressor of stem-loop protein 1 / type: protein_or_peptide / ID: 30 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MAPVVISESE EDEDRVAITR RTKRQVHFDG EGDDRVDQQQ QQHSSSHRDR DKHVQRKKKK RLSNRNLQGS NGGYAWEDEI KRSWDLVKVD DEGDMASLVA SIVEARKKRT AKKNITPYQR GIIRSLILTL DCSEAMLEKD LRPNRHAMII QYAIDFVHEF FDQNPISQMG ...String:
MAPVVISESE EDEDRVAITR RTKRQVHFDG EGDDRVDQQQ QQHSSSHRDR DKHVQRKKKK RLSNRNLQGS NGGYAWEDEI KRSWDLVKVD DEGDMASLVA SIVEARKKRT AKKNITPYQR GIIRSLILTL DCSEAMLEKD LRPNRHAMII QYAIDFVHEF FDQNPISQMG IIIMRNGLAQ LVSQVSGNPQ DHIDALKSIR KQEPKGNPSL QNALEMARGL LLPVPAHCTR EVLIVFGSLS TTDPGDIHQT IDSLVSEKIR VKVLGLSAQV AICKELCKAT NYGDESFYKI LLDETHLKEL FNEAVTPLPV NKINKGFTLV KMGFPTRIFE DTPTFCSCHS KLVYGGYFCP NCHSKVCSLP TVCPCCDLML ILSTHLARSY HHLMPLKTFA EVPTTEKFRS EDCFSCQSRF PILKNHKNGK LLTSSRYRCE DCKQEFCVDC DVFIHEILHN CPGCESKPVI T

+
Macromolecule #31: DNA repair helicase RAD25

MacromoleculeName: DNA repair helicase RAD25 / type: protein_or_peptide / ID: 31 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV TGLKKPRKKT KSSRHTAADS SMNQMDAKDK ALLQDTNSDI PADFVPDSVS GMFRSHDFSY LRLRPDHASR PLWISPSDGR IILESFSPLA EQAQDFLVTI AEPISRPSHI ...String:
MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV TGLKKPRKKT KSSRHTAADS SMNQMDAKDK ALLQDTNSDI PADFVPDSVS GMFRSHDFSY LRLRPDHASR PLWISPSDGR IILESFSPLA EQAQDFLVTI AEPISRPSHI HEYKITAYSL YAAVSVGLET DDIISVLDRL SKVPVAESII NFIKGATISY GKVKLVIKHN RYFVETTQAD ILQMLLNDSV IGPLRIDSDH QVQPPEDVLQ QQLQQTAGKP ATNVNPNDVE AVFSAVIGGD NEREEEDDDI DAVHSFEIAN ESVEVVKKRC QEIDYPVLEE YDFRNDHRNP DLDIDLKPST QIRPYQEKSL SKMFGNGRAR SGIIVLPCGA GKTLVGITAA CTIKKSVIVL CTSSVSVMQW RQQFLQWCTL QPENCAVFTS DNKEMFQTES GLVVSTYSMV ANTRNRSHDS QKVMDFLTGR EWGFIILDEV HVVPAAMFRR VVSTIAAHAK LGLTATLVRE DDKIGDLNFL IGPKLYEANW MELSQKGHIA NVQCAEVWCP MTAEFYQEYL RETARKRMLL YIMNPTKFQA CQFLIQYHER RGDKIIVFSD NVYALQEYAL KMGKPFIYGS TPQQERMNIL QNFQYNDQIN TIFLSKVGDT SIDLPEATCL IQISSHYGSR RQEAQRLGRI LRAKRRNDEG FNAFFYSLVS KDTQEMYYST KRQAFLVDQG YAFKVITHLH GMENIPNLAY ASPRERRELL QEVLLKNEEA AGIEVGDDAD NSVGRGSNGH KRFKSKAVRG EGSLSGLAGG EDMAYMEYST NKNKELKEHH PLIRKMYYKN LKK

+
Macromolecule #32: Mediator of RNA polymerase II transcription subunit 1

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 32 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFDY FNQRDGEHEK SNILLNSLTK YPDLKAFHNN LKFLYLLDAY SHIESDSTSH NNGSSDKSLD SSNASFNNQG KLDLFKYFTE ...String:
MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFDY FNQRDGEHEK SNILLNSLTK YPDLKAFHNN LKFLYLLDAY SHIESDSTSH NNGSSDKSLD SSNASFNNQG KLDLFKYFTE LSHYIRQCFQ DNCCDFKVRT NLNDKFGIYI LTQGINGKEV PLAKIYLEEN KSDSQYRFYE YIYSQETKSW INESAENFSN GISLVMEIVA NAKESNYTDL IWFPEDFISP ELIIDKVTCS SNSSSSPPII DLFSNNNYNS RIQLMNDFTT KLINIKKFDI SNDNLDLISE ILKWVQWSRI VLQNVFKLVS TPSSNSNSSE LEPDYQAPFS TSTKDKNSST SNTEPIPRSN RHGSVVEASR RRRSSTNKSK RPSITEAMML KEEGLQQFNL HEILSEPAIE EENGDSIKEH STTMDGANDL GFTASVSNQE NAGTDIVMED HGVLQGTSQN YGTATADDAD IEMKDVSSKP SKPESSVLQL IVSEDHIILD TISECNLYDD VKCWSKFIEK FQDIVS

+
Macromolecule #33: Mediator of RNA polymerase II transcription subunit 4

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 33 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAEY DNIYRNLQKI DKDSEELDSK TRKILEILNE CHDELKALPM LEQVEFEKNT ILQQRSKINS TELLDYATKL SKFTKIPPTF ...String:
MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAEY DNIYRNLQKI DKDSEELDSK TRKILEILNE CHDELKALPM LEQVEFEKNT ILQQRSKINS TELLDYATKL SKFTKIPPTF DKGAVGPNNF IWPAEDALRR GMLAMASLHS KELTRIPGEE VEETEVPTVP PSQSEEQKGQ MAKKEGTPKT DSFIFDGTAK EVGDEADNTK DKEKEENNDD ALDLDLDLFD PDDF

+
Macromolecule #34: Mediator of RNA polymerase II transcription subunit 6

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 34 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPAR RQILFKYPMY MQLEEELMKL DGTEYVLSSV REPDFWVIRK QRRTNNSGVG SAKGPEIIPL QDYYIIGANI YQSPTIFKIV ...String:
MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPAR RQILFKYPMY MQLEEELMKL DGTEYVLSSV REPDFWVIRK QRRTNNSGVG SAKGPEIIPL QDYYIIGANI YQSPTIFKIV QSRLMSTSYH LNSTLESLYD LIEFQPSQGV HYKVPTDTST TATAATNGNN AGGGSNKSSV RPTGGANMAT VPSTTNVNMT VNTMGTGGQT IDNGTGRTGN GNMGITTEML DKLMVTSIRS TPNYI

+
Macromolecule #35: Mediator of RNA polymerase II transcription subunit 7

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 35 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL YKKSTENEST NYQYKIQELR KLLKSLLLNY LELIGVLSIN PDMYERKVEN IRTILVNIHH LLNEYRPHQS ...String:
MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL YKKSTENEST NYQYKIQELR KLLKSLLLNY LELIGVLSIN PDMYERKVEN IRTILVNIHH LLNEYRPHQS RESLIMLLEE QLEYKRGEIR EIEQVCKQVH DKLTSIQDTL RTGSQSPPSS SQ

+
Macromolecule #36: Mediator of RNA polymerase II transcription subunit 8

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 36 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ QDREITNWAR TTFRNEYGKH ...String:
MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ QDREITNWAR TTFRNEYGKH DFKNEESLSE EHASLLVRDS KPSKPFNVDD VLKFTFTGEK PIITGSTSTS SSN

+
Macromolecule #37: Mediator of RNA polymerase II transcription subunit 9

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 37 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MNLQNNVLNQ IHQILLPTNP TLDKPNAEAT KEEFSSAENR DEKDYLTNQQ PKNLSTPSTS SNGEFIPHIF YSLHQIRKDP NNLSNQLETL TGSIRHRLKL CKSLISENED TKDLLSKSPS EWQDIIHQRE QELQIKRDVL DDLYRKLQR

+
Macromolecule #38: Mediator of RNA polymerase II transcription subunit 10

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 10
type: protein_or_peptide / ID: 38 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI DNVSNTH

+
Macromolecule #39: Mediator of RNA polymerase II transcription subunit 11

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 11
type: protein_or_peptide / ID: 39 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MQPPYIQERL KSLNDIETQL CSMLQEASQV TFIFGELKRG NESVKPQFEN HVKQFYERLD KSTTQLRKEI QLLDENVGTR LLPINVNKKA LGQDTEKMEE QLDLLSAILD PSKSK

+
Macromolecule #40: Mediator of RNA polymerase II transcription subunit 14

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 14
type: protein_or_peptide / ID: 40 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE SLEMVPKDT SAATMTSAPP PALPHVEINQ VSLALVIRNL TVFTMKELAQ YMKTNVHTQA N EPNSAKKI RFLQLIIFLR TQFLKLYVLV KWTRTIKQNN FHVLIDLLNW ...String:
MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE SLEMVPKDT SAATMTSAPP PALPHVEINQ VSLALVIRNL TVFTMKELAQ YMKTNVHTQA N EPNSAKKI RFLQLIIFLR TQFLKLYVLV KWTRTIKQNN FHVLIDLLNW FRTTNMNVNN CI WALKSSL NSMTNAKLPN VDLVTALEVL SLGRPNLPTH NFKLSGVSNS MDMVDGMAKV PIG LILQRL KDLNLTVSIK IALMNIPKPL NSYHIKNGRI YFTVPNEFEI QLSTVNRQSP LFFV DLKLL FNTEAEQTVS AVTEATSTNG DSENNEENSS SNGNNLPLNK PRLEKLINEI LLKSN DPLL SLYNFLHKYV LTLQLYMVHR EFLKLANGGK FSKSNLIHNY DSKKSTITVR YWLNGK MDS KGKITIGIQR TTESLILKWD NQSASRAKNM PVIYNNIVSN IEGILDEIMF NHARIIR SE LLARDIFQED EENSDVLLFQ LPTTCVSMAP IQLKIDLLSG QFYFRNPTPL LSNYASKI N RAEGPEELAR ILQQLKLDKI IHVLTTMFEN TGWSCSRIIK IDKPIRTQVN TGGESVVKK EDNKYAIAGN STTNSDVSLL LQRDLFIRLP HWPLNWYLIL SIISSKTSCV VEKRIGKIVS QRGKWNLKY LDNSNVMTVK LESITYQKIM ILQRTILNRI INHMLIDSLN QL

+
Macromolecule #41: Mediator of RNA polymerase II transcription subunit 17

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 17
type: protein_or_peptide / ID: 41 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSNP HEIYGSMPLE QLIPIILRQR GPGFKFVDLN EKELQNEIKQ LGSDSSDGHN SEKKDTDGAD ENVQIGEDFM EVDYEDKDNP ...String:
MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSNP HEIYGSMPLE QLIPIILRQR GPGFKFVDLN EKELQNEIKQ LGSDSSDGHN SEKKDTDGAD ENVQIGEDFM EVDYEDKDNP VDSRNETDHK TNENGETDDN IETVMTQEQF VKRRRDMLEH INLAMNESSL ALEFVSLLLS SVKESTGMSS MSPFLRKVVK PSSLNSDKIP YVAPTKKEYI ELDILNKGWK LQSLNESKDL LRASFNKLSS ILQNEHDYWN KIMQSISNKD VIFKIRDRTS GQKLLAIKYG YEDSGSTYKH DRGIANIRNN IESQNLDLIP HSSSVFKGTD FVHSVKKFLR VRIFTKIESE DDYILSGESV MDRDSESEEA ETKDIRKQIQ LLKKIIFEKE LMYQIKKECA LLISYGVSIE NENKVIIELP NEKFEIELLS LDDDSIVNHE QDLPKINDKR ANLMLVMLRL LLVVIFKKTL RSRISSPHGL INLNVDDDIL IIRPILGKVR FANYKLLLKK IIKDYVLDIV PGSSITETEV EREQPQENKN IDDENITKLN KEIRAFDKLL NIPRRELKIN LPLTEHKSPN LSLMLESPNY CNALIHIKFS AGTEANAVSF DTTFSDFKEV EDFLHFIVAE YIQQKKV

+
Macromolecule #42: Mediator of RNA polymerase II transcription subunit 18

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 18
type: protein_or_peptide / ID: 42 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRIL KSFTNDKIPL NYAMTRNILH NTVPQVTNFN STNEDQNNSK HTEDTVNESR NSDDIIDVDM DASPAPSNES CSPWSLQISD ...String:
MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRIL KSFTNDKIPL NYAMTRNILH NTVPQVTNFN STNEDQNNSK HTEDTVNESR NSDDIIDVDM DASPAPSNES CSPWSLQISD IPAAGNNRSV SMQTIAETII LSSAGKNSSV SSLMNGLGYV FEFQYLTIGV KFFMKHGLIL ELQKIWQIEE AGNSQITSGG FLLKAYINVS RGTDIDRINY TETALMNLKK ELQGYIELSV PDRQSMDSRV AHGNILI

+
Macromolecule #43: Mediator of RNA polymerase II transcription subunit 19

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 19
type: protein_or_peptide / ID: 43 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL RKSYKNQIAD LSGKFSTIPT RENGKGGQIA HILFQNNPDM MIQPPQQGQN MSEQQWREQL RNRDIALFQP PNFDWDLCSS VLSQFERSYP SEFANQNQGG AQAPFDIDDL ...String:
MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL RKSYKNQIAD LSGKFSTIPT RENGKGGQIA HILFQNNPDM MIQPPQQGQN MSEQQWREQL RNRDIALFQP PNFDWDLCSS VLSQFERSYP SEFANQNQGG AQAPFDIDDL AFDLDGTGKS QSGSNSGNNS KKRKNKSSGS SMATPTHSDS HEDMKRRRLE

+
Macromolecule #44: Mediator of RNA polymerase II transcription subunit 20

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 20
type: protein_or_peptide / ID: 44 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString: MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG LLIELQADEA GEFETKIAGI ...String:
MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL AYQYVRALEL

+
Macromolecule #45: Mediator of RNA polymerase II transcription subunit 21

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 21
type: protein_or_peptide / ID: 45 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MTDRLTQLQI CLDQMTEQFC ATLNYIDKNH GFERLTVNEP QMSDKHATVV PPEEFSNTID ELSTDIILKT RQINKLIDSL PGVDVSAEEQ LRKIDMLQKK LVEVEDEKIE AIKKKEKLLR HVDSLIEDFV DGIANSKKST

+
Macromolecule #46: Mediator of RNA polymerase II transcription subunit 22

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 22
type: protein_or_peptide / ID: 46 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MSNQALYEKL EQTRTILSVK LAELINMTTI ADRNDDDEGS FAQENSELAV ATTSVMMVNN QTMQLIKNVQ DLLILTRSIK EKWLLNQIPV TEHSKVTRFD EKQIEELLDN CIETFVAEKT T

+
Macromolecule #47: Mediator of RNA polymerase II transcription subunit 31

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 31
type: protein_or_peptide / ID: 47 / Enantiomer: LEVO
Source (natural)Organism: yeast (yeast)
SequenceString:
MSSTNGNAPA TPSSDQNPLP TRFEVELEFI QSLANIQYVT YLLTQQQIWK SPNFKNYLKY LEYWCNPPYS QCIVYPNCLF ILKLLNGFME SAIVNEDGLL EGLDELPKII QLQGPQWMNE MVERWAN

+
Macromolecule #48: 108 bp HIS4 promoter fragment

MacromoleculeName: 108 bp HIS4 promoter fragment / type: dna / ID: 48 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString:
ATATGACTCA TGAACAGTAG TATGCTGTGT ATATAATAGC TATGGAACGT TCGATTCACC TCCGATGTGT GTTGTACATA CATAAAAATA TCATAGCACA ACTGCGCT

-
Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
80.0 mMCH3CO2Kpotassium acetate
20.0 mM(HOCH2)3CNH2Tris
2.0 mMCH3CO2Mgmagnesium acetate
5.0 mMC4H10O2S2Dithiothreitol

Details: 80 mM potassium acetate, 20 mM Tris, pH 7.5, 2 mM magnesium acetate, 5 mM DTT
GridModel: Quantifoil R2/1, R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV
DetailsFor cryo-EM, samples were chemically stabilized by fixation in glycerol gradients with increasing concentrations of glutaraldehyde (0-0.1%). Peak gradient fractions (~0.2 mg/ml) containing glutaraldhyde were quenched upon the addition of 50 mM Tris-HCl (pH 7.5) before flash-freezing and storage under liquid nitrogen.

-
Electron microscopy

MicroscopeFEI TECNAI F20
Image recordingFilm or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Average electron dose: 15.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.26 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
Sample stageSpecimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company

+
Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 3)
Startup modelType of model: OTHER
Details: Initial Model generated using EMAN 2.1 e2initialmodel.py program using a small subset of 2D class averages generated from Relion 1.4 Class2D.
Final reconstructionResolution.type: BY AUTHOR / Resolution: 15.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 170600
Initial angle assignmentType: RANDOM ASSIGNMENT / Software - Name: RELION (ver. 1.4)
Final angle assignmentType: OTHER / Software - Name: RELION (ver. 1.4) / Details: Bayesian Maximum Likelihood Approach.
Final 3D classificationSoftware - Name: RELION (ver. 1.4)
FSC plot (resolution estimation)

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  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

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Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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