|Entry||Database: EMDB / ID: EMD-4108|
|Title||Cryo-EM of bovine respirasome|
|Biological species||Bos taurus (cattle)|
|Method||single particle reconstruction / cryo EM / Resolution: 10.4 Å|
|Authors||Sousa JS / Mills DJ / Vonck J / Kuehlbrandt W|
|Citation||Journal: Elife / Year: 2016|
Title: Functional asymmetry and electron flow in the bovine respirasome.
Authors: Joana S Sousa / Deryck J Mills / Janet Vonck / Werner Kühlbrandt /
Abstract: Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex IIIIIV from bovine heart ...Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex IIIIIV from bovine heart mitochondria by cryo-EM at 9 Å resolution. Most protein-protein contacts between complex I, III and IV in the membrane are mediated by supernumerary subunits. Of the two Rieske iron-sulfur cluster domains in the complex III dimer, one is resolved, indicating that this domain is immobile and unable to transfer electrons. The central position of the active complex III monomer between complex I and IV in the respirasome is optimal for accepting reduced quinone from complex I over a short diffusion distance of 11 nm, and delivering reduced cytochrome to complex IV. The functional asymmetry of complex III provides strong evidence for directed electron flow from complex I to complex IV through the active complex III monomer in the mammalian supercomplex.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_4108.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.77 Å|
|Symmetry||Space group: 1|
CCP4 map header:
|Entire||Name: Respirasome / Details: supercomplex of complex I, III and IV / Number of components: 1|
-Component #1: protein, Respirasome
|Protein||Name: Respirasome / Details: supercomplex of complex I, III and IV / Recombinant expression: No|
|Mass||Theoretical: 1.7 MDa|
|Source||Species: Bos taurus (cattle)|
|Specimen||Specimen state: Particle / Method: cryo EM|
|Sample solution||pH: 7.7|
|Vitrification||Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 283 K / Humidity: 90 % / Details: 10 s blotting.|
-Electron microscopy imaging
Model: Tecnai Polara / Image courtesy: FEI Company
|Imaging||Microscope: FEI POLARA 300|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 70 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 59000.0 X (nominal), 57797.0 X (calibrated) / Cs: 2.2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1.3 - 4.3 nm|
|Specimen Holder||Model: OTHER|
|Image acquisition||Number of digital images: 3592 / Sampling size: 14 µm / Details: Falcon III|
|Processing||Method: single particle reconstruction / Number of projections: 10111|
|3D reconstruction||Software: RELION / Resolution: 10.4 Å / Resolution method: FSC 0.143 CUT-OFF|
-Atomic model buiding
|Modeling #1||Refinement protocol: rigid body / Refinement space: REAL|
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