EMDB-12610: Human Mediator with RNA Polymerase II Pre-initiation complex

Basic information

• Entry: Database: EMDB / ID: EMD-12610
• Title: Human Mediator with RNA Polymerase II Pre-initiation complex

Sample

• Complex: Human Mediator with RNA Polymerase II Pre-initiation complex
  • Complex: Pol Complex and Mediator of RNA polymerase II transcription
    • Protein or peptide: x 34 types
  • Complex: DNA-directed RNA polymerasePolymerase
    • Protein or peptide: x 12 types
  • Complex: NT and Template
    • DNA: x 2 types
  • Complex: unassigned peptides (MED29 or MED30)
    • Protein or peptide: x 2 types
  • Complex: Transcription initiation factors
    • Protein or peptide: x 8 types
• Ligand: x 3 types

Function / homology

Function:

negative regulation of smooth muscle cell differentiation / MMXD complex / core TFIIH complex portion of holo TFIIH complex / positive regulation of DNA helicase activity / negative regulation of DNA helicase activity / Cytosolic iron-sulfur cluster assembly / nucleotide-excision repair, DNA duplex unwinding / central nervous system myelin formation / positive regulation of mitotic recombination / core mediator complex / positive regulation of core promoter binding / meiotic sister chromatid cohesion / RNA polymerase II core complex assembly / hair cell differentiation / ventricular system development / hair follicle maturation / cyclin-dependent protein kinase activating kinase holoenzyme complex / RNA polymerase transcription factor SL1 complex / snRNA transcription by RNA polymerase II / nucleotide-excision repair factor 3 complex / transcription factor TFIIE complex / nucleotide-excision repair, preincision complex assembly / phosphatase activator activity / UV protection / CAK-ERCC2 complex / transcription factor TFIIK complex / RNA polymerase III general transcription initiation factor activity / embryonic cleavage / transcription open complex formation at RNA polymerase II promoter / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / mediator complex / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / RNA Polymerase III Transcription Initiation From Type 3 Promoter / transcription factor TFIIF complex / 5'-3' DNA helicase activity / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Generic Transcription Pathway / adult heart development / RNA Polymerase III Abortive And Retractive Initiation / transcription factor TFIIA complex / G protein-coupled receptor internalization / : / female germ cell nucleus / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / male pronucleus / female pronucleus / cyclin-dependent protein serine/threonine kinase activator activity / nuclear vitamin D receptor binding / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / germinal vesicle / [RNA-polymerase]-subunit kinase / RNA polymerase II general transcription initiation factor binding / limb development / 3'-5' DNA helicase activity / nuclear thyroid hormone receptor binding / transcription preinitiation complex / RNA Polymerase I Transcription Termination / regulation of mitotic cell cycle phase transition / cyclin-dependent protein serine/threonine kinase regulator activity / : / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / Viral Messenger RNA Synthesis / hematopoietic stem cell proliferation / Signaling by FGFR2 IIIa TM / termination of RNA polymerase II transcription / cell division site / cortical actin cytoskeleton / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE

Domain/homology:

Mediator complex subunit 28 / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med19, metazoa / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator of RNA pol II transcription subunit 19 / Mediator complex subunit 30 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med7 superfmaily / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription factor TFIIE alpha subunit, C-terminal / C-terminal general transcription factor TFIIE alpha / CyclinH/Ccl1 / Cyclin-dependent kinase 7 / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain / TFIIH C1-like domain / TFIIH p62 subunit, N-terminal / TFIIH subunit Tfb1/GTF2H1 / TFIIH p62 subunit, N-terminal domain / BSD domain / BSD domain superfamily / BSD domain / BSD domain profile. / domain in transcription factors and synapse-associated proteins / RAD3/XPD family / Helicase XPB/Ssl2 / Helical and beta-bridge domain / ERCC3/RAD25/XPB helicase, C-terminal domain / Helicase XPB/Ssl2, N-terminal domain / Helical and beta-bridge domain / Helicase conserved C-terminal domain / ERCC3/RAD25/XPB C-terminal helicase / Transcription factor TFIIH subunit p52/Tfb2 / ATP-dependent helicase Rad3/Chl1-like / Transcription factor Tfb2, C-terminal domain / Transcription factor Tfb2 / Transcription factor Tfb2 (p52) C-terminal domain / Transcription factor TFIIE beta subunit, DNA-binding domain / Transcription initiation factor TFIIE, beta subunit / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / TFA2 Winged helix domain 2 / TFIIE beta central core DNA-binding domain profile. / TFIIH subunit TTDA/Tfb5 / TFB5-like superfamily / Transcription factor TFIIH complex subunit Tfb5 / Transcription factor TFIIH complex subunit Tfb5 / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Helicase superfamily 1/2, DinG/Rad3-like / Helicase-like, DEXD box c2 type / ATP-dependent helicase, C-terminal / DEAD2 / Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-type / Cyclin, C-terminal domain 2 / DEAD_2 / Helicase C-terminal domain / Cyclin C-terminal domain / Superfamilies 1 and 2 helicase ATP-binding type-2 domain profile. / DEXDc2

Component:

RNA polymerase II subunit D / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase II subunit RPB7 / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 6 / General transcription factor IIF subunit 2 / General transcription and DNA repair factor IIH helicase subunit XPD / General transcription and DNA repair factor IIH helicase subunit XPB / TATA-box-binding protein / General transcription factor IIE subunit 1 / Transcription initiation factor IIE subunit beta / General transcription factor IIH subunit 1 / General transcription factor IIF subunit 1 / Cyclin-dependent kinase 7 / Cyclin-H / CDK-activating kinase assembly factor MAT1 / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 21 / General transcription factor IIH subunit 2 / General transcription factor IIH subunit 3 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 27 / General transcription factor IIH subunit 5 / General transcription factor IIH subunit 4 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 31

• Biological species: Homo sapiens (human) / Sus scrofa (pig) / Human mastadenovirus C / Trichoplusia ni (cabbage looper) / Pig (pig)
• Method: single particle reconstruction / cryo EM / Resolution: 4.5 Å
• Authors: Rengachari S / Schilbach S / Aibara S / Cramer P

Funding support

Germany, European Union, 5 items

Organization	Grant number	Country
German Research Foundation (DFG)	EXC 2067/1 39072994	Germany
German Research Foundation (DFG)	SFB860	Germany
German Research Foundation (DFG)	SPP2191	Germany
European Research Council (ERC)	882357	European Union
H2020 Marie Curie Actions of the European Commission	894862	European Union

• Citation: Journal: Nature / Year: 2021; Title: Structures of mammalian RNA polymerase II pre-initiation complexes.; Authors: Shintaro Aibara / Sandra Schilbach / Patrick Cramer / ; Abstract: The initiation of transcription is a focal point for the regulation of gene activity during mammalian cell differentiation and development. To initiate transcription, RNA polymerase II (Pol II) assembles with general transcription factors into a pre-initiation complex (PIC) that opens promoter DNA. Previous work provided the molecular architecture of the yeast and human PIC and a topological model for DNA opening by the general transcription factor TFIIH. Here we report the high-resolution cryo-electron microscopy structure of PIC comprising human general factors and Sus scrofa domesticus Pol II, which is 99.9% identical to human Pol II. We determine the structures of PIC with closed and opened promoter DNA at 2.5-2.8 Å resolution, and resolve the structure of TFIIH at 2.9-4.0 Å resolution. We capture the TFIIH translocase XPB in the pre- and post-translocation states, and show that XPB induces and propagates a DNA twist to initiate the opening of DNA approximately 30 base pairs downstream of the TATA box. We also provide evidence that DNA opening occurs in two steps and leads to the detachment of TFIIH from the core PIC, which may stop DNA twisting and enable RNA chain initiation.

History

Deposition	Mar 15, 2021	-
Header (metadata) release	May 5, 2021	-
Map release	May 5, 2021	-
Update	Jun 16, 2021	-
Current status	Jun 16, 2021	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_12610.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.05 Å

Density

Contour Level	By AUTHOR: 3.0 / Movie #1: 3
Minimum - Maximum	-17.335718 - 40.087654
Average (Standard dev.)	-3.3155442e-12 (±1.0)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 440.99997 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.05	1.05	1.05
M x/y/z	420	420	420
origin x/y/z	0.000	0.000	0.000
length x/y/z	441.000	441.000	441.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	420	420	420
MAP C/R/S	3	2	1
start NC/NR/NS	0	0	0
NC/NR/NS	420	420	420
D min/max/mean	-17.336	40.088	-0.000

Supplemental data

Mask #1

• File: emd_12610_msk_1.map

Additional map: #1

• File: emd_12610_additional_1.map

Half map: #2

• File: emd_12610_half_map_1.map

Half map: #1

• File: emd_12610_half_map_2.map

Sample components

Entire : Human Mediator with RNA Polymerase II Pre-initiation complex

• Entire: Name: Human Mediator with RNA Polymerase II Pre-initiation complex

Components

• Complex: Human Mediator with RNA Polymerase II Pre-initiation complex
  • Complex: Pol Complex and Mediator of RNA polymerase II transcription
    • Protein or peptide: TFIIH basal transcription factor complex helicase XPD subunit
    • Protein or peptide: General transcription factor IIH subunit 1
    • Protein or peptide: General transcription factor IIH subunit 4
    • Protein or peptide: CDK-activating kinase assembly factor MAT1
    • Protein or peptide: General transcription factor IIH subunit 3
    • Protein or peptide: General transcription factor IIH subunit 5
    • Protein or peptide: General transcription factor IIH subunit 2
    • Protein or peptide: General transcription and DNA repair factor IIH helicase subunit XPB
    • Protein or peptide: Cyclin-dependent kinase 7
    • Protein or peptide: Cyclin-H
    • Protein or peptide: TATA-box-binding protein
    • Protein or peptide: Unassigned peptide, likely XPB
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
    • Protein or peptide: unassigned peptide (MED29 or MED30)
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
    • Protein or peptide: unassigned peptide (MED14)
    • Protein or peptide: unassigned peptide (MED6)
    • Protein or peptide: unassigned peptide (MED17)
  • Complex: DNA-directed RNA polymerasePolymerase
    • Protein or peptide: DNA-directed RNA polymerase subunitPolymerase
    • Protein or peptide: DNA-directed RNA polymerase subunit betaPolymerase
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3Polymerase
    • Protein or peptide: RPOL4c domain-containing protein
    • Protein or peptide: DNA-directed RNA polymerase II subunit EPolymerase
    • Protein or peptide: DNA-directed RNA polymerase II subunit FPolymerase
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7Polymerase
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3RNA polymerase
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9Polymerase
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5RNA polymerase
    • Protein or peptide: RNA_pol_L_2 domain-containing protein
    • Protein or peptide: RNA polymerase II subunit K
  • Complex: NT and Template
    • DNA: NT
    • DNA: TEMPLATE
  • Complex: unassigned peptides (MED29 or MED30)
    • Protein or peptide: unassigned peptide (MED29 or MED30)
    • Protein or peptide: unassigned peptide (MED29 or MED30)
  • Complex: Transcription initiation factors
    • Protein or peptide: Transcription initiation factor IIB
    • Protein or peptide: General transcription factor IIF subunit 1
    • Protein or peptide: General transcription factor IIF subunit 2
    • Protein or peptide: Transcription initiation factor IIA subunit 1
    • Protein or peptide: Transcription initiation factor IIA subunit 2
    • Protein or peptide: General transcription factor IIE subunit 1
    • Protein or peptide: Transcription initiation factor IIE subunit beta
    • Protein or peptide: Unassigned peptide, likely TFIIE-beta
• Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION

Supramolecule #1: Human Mediator with RNA Polymerase II Pre-initiation complex

• Supramolecule: Name: Human Mediator with RNA Polymerase II Pre-initiation complex; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58

Supramolecule #2: Pol Complex and Mediator of RNA polymerase II transcription

• Supramolecule: Name: Pol Complex and Mediator of RNA polymerase II transcription; type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#10, #25, #34-#56
• Source (natural): Organism: Homo sapiens (human)
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Supramolecule #3: DNA-directed RNA polymerase

• Supramolecule: Name: DNA-directed RNA polymerase / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #11-#22
• Source (natural): Organism: Sus scrofa (pig)

Supramolecule #4: NT and Template

• Supramolecule: Name: NT and Template / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #24, #28
• Source (natural): Organism: Human mastadenovirus C
• Recombinant expression: Organism: synthetic construct (others)

Supramolecule #5: unassigned peptides (MED29 or MED30)

• Supramolecule: Name: unassigned peptides (MED29 or MED30) / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #57-#58
• Source (natural): Organism: Trichoplusia ni (cabbage looper)

Supramolecule #6: Transcription initiation factors

• Supramolecule: Name: Transcription initiation factors / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #23, #26-#27, #29-#33
• Source (natural): Organism: Homo sapiens (human)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Macromolecule #1: TFIIH basal transcription factor complex helicase XPD subunit

• Macromolecule: Name: TFIIH basal transcription factor complex helicase XPD subunit; type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 87.021078 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MKLNVDGLLV YFPYDYIYPE QFSYMRELKR TLDAKGHGVL EMPSGTGKTV SLLALIMAYQ RAYPLEVTKL IYCSRTVPEI EKVIEELRK LLNFYEKQEG EKLPFLGLAL SSRKNLCIHP EVTPLRFGKD VDGKCHSLTA SYVRAQYQHD TSLPHCRFYE E FDAHGREV PLPAGIYNLD DLKALGRRQG WCPYFLARYS ILHANVVVYS YHYLLDPKIA DLVSKELARK AVVVFDEAHN ID NVCIDSM SVNLTRRTLD RCQGNLETLQ KTVLRIKETD EQRLRDEYRR LVEGLREASA ARETDAHLAN PVLPDEVLQE AVP GSIRTA EHFLGFLRRL LEYVKWRLRV QHVVQESPPA FLSGLAQRVC IQRKPLRFCA ERLRSLLHTL EITDLADFSP LTLL ANFAT LVSTYAKGFT IIIEPFDDRT PTIANPILHF SCMDASLAIK PVFERFQSVI ITSGTLSPLD IYPKILDFHP VTMAT FTMT LARVCLCPMI IGRGNDQVAI SSKFETREDI AVIRNYGNLL LEMSAVVPDG IVAFFTSYQY MESTVASWYE QGILEN IQR NKLLFIETQD GAETSVALEK YQEACENGRG AILLSVARGK VSEGIDFVHH YGRAVIMFGV PYVYTQSRIL KARLEYL RD QFQIRENDFL TFDAMRHAAQ CVGRAIRGKT DYGLMVFADK RFARGDKRGK LPRWIQEHLT DANLNLTVDE GVQVAKYF L RQMAQPFHRE DQLGLSLLSL EQLESEETLK RIEQIAQQL

Macromolecule #2: General transcription factor IIH subunit 1

• Macromolecule: Name: General transcription factor IIH subunit 1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 62.116492 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MATSSEEVLL IVKKVRQKKQ DGALYLMAER IAWAPEGKDR FTISHMYADI KCQKISPEGK AKIQLQLVLH AGDTTNFHFS NESTAVKER DAVKDLLQQL LPKFKRKANK ELEEKNRMLQ EDPVLFQLYK DLVVSQVISA EEFWANRLNV NATDSSSTSN H KQDVGISA AFLADVRPQT DGCNGLRYNL TSDIIESIFR TYPAVKMKYA ENVPHNMTEK EFWTRFFQSH YFHRDRLNTG SK DLFAECA KIDEKGLKTM VSLGVKNPLL DLTALEDKPL DEGYGISSVP SASNSKSIKE NSNAAIIKRF NHHSAMVLAA GLR KQEAQN EQTSEPSNMD GNSGDADCFQ PAVKRAKLQE SIEYEDLGKN NSVKTIALNL KKSDRYYHGP TPIQSLQYAT SQDI INSFQ SIRQEMEAYT PKLTQVLSSS AASSTITALS PGGALMQGGT QQAINQMVPN DIQSELKHLY VAVGELLRHF WSCFP VNTP FLEEKVVKMK SNLERFQVTK LCPFQEKIRR QYLSTNLVSH IEEMLQTAYN KLHTWQSRRL MKKT

Macromolecule #3: General transcription factor IIH subunit 4

• Macromolecule: Name: General transcription factor IIH subunit 4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.245156 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MESTPSRGLN RVHLQCRNLQ EFLGGLSPGV LDRLYGHPAT CLAVFRELPS LAKNWVMRML FLEQPLPQAA VALWVKKEFS KAQEESTGL LSGLRIWHTQ LLPGGLQGLI LNPIFRQNLR IALLGGGKAW SDDTSQLGPD KHARDVPSLD KYAEERWEVV L HFMVGSPS AAVSQDLAQL LSQAGLMKST EPGEPPCITS AGFQFLLLDT PAQLWYFMLQ YLQTAQSRGM DLVEILSFLF QL SFSTLGK DYSVEGMSDS LLNFLQHLRE FGLVFQRKRK SRRYYPTRLA INLSSGVSGA GGTVHQPGFI VVETNYRLYA YTE SELQIA LIALFSEMLY RFPNMVVAQV TRESVQQAIA SGITAQQIIH FLRTRAHPVM LKQTPVLPPT ITDQIRLWEL ERDR LRFTE GVLYNQFLSQ VDFELLLAHA RELGVLVFEN SAKRLMVVTP AGHSDVKRFW KRQKHSS

Macromolecule #4: CDK-activating kinase assembly factor MAT1

• Macromolecule: Name: CDK-activating kinase assembly factor MAT1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.873965 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDF PSLREYNDFL EEVEEIVFNL TNNVDLDNTK KKMEIYQKEN KDVIQKNKLK LTREQEELEE ALEVERQENE Q RRLFIQKE EQLQQILKRK NKQAFLDELE SSDLPVALLL AQHKDRSTQL EMQLEKPKPV KPVTFSTGIK MGQHISLAPI HK LEEALYE YQPLQIETYG PHVPELEMLG RLGYLNHVRA ASPQDLAGGY TSSLACHRAL QDAFSGLFWQ PS

Macromolecule #5: General transcription factor IIH subunit 3

• Macromolecule: Name: General transcription factor IIH subunit 3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.416008 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI ASHIQESRFL YPGKNGRLGD FFGDPGNPP EFNPSGSKDG KYELLTSANE VIVEEIKDLM TKSDIKGQHT ETLLAGSLAK ALCYIHRMNK EVKDNQEMKS R ILVIKAAE DSALQYMNFM NVIFAAQKQN ILIDACVLDS DSGLLQQACD ITGGLYLKVP QMPSLLQYLL WVFLPDQDQR SQ LILPPPV HVDYRAACFC HRNLIEIGYV CSVCLSIFCN FSPICTTCET AFKISLPPVL KAKKKKLKVS A

Macromolecule #6: General transcription factor IIH subunit 5

• Macromolecule: Name: General transcription factor IIH subunit 5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.060362 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MVNVLKGVLI ECDPAMKQFL LYLDESNALG KKFIIQDIDD THVFVIAELV NVLQERVGEL MDQNAFSLTQ K

Macromolecule #7: General transcription factor IIH subunit 2

• Macromolecule: Name: General transcription factor IIH subunit 2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.481996 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLE YFVEEYFDQN PISQIGIIVT KSKRAEKLTE LSGNPRKHIT SLKKAVDMTC HGEPSLYNSL SIAMQTLKHM P GHTSREVL IIFSSLTTCD PSNIYDLIKT LKAAKIRVSV IGLSAEVRVC TVLARETGGT YHVILDESHY KELLTHHVSP PP ASSSSEC SLIRMGFPQH TIASLSDQDA KPSFSMAHLD GNTEPGLTLG GYFCPQCRAK YCELPVECKI CGLTLVSAPH LAR SYHHLF PLDAFQEIPL EEYNGERFCY GCQGELKDQH VYVCAVCQNV FCVDCDVFVH DSLHCCPGCI HKIPAPSGV

Macromolecule #8: General transcription and DNA repair factor IIH helicase subunit XPB

• Macromolecule: Name: General transcription and DNA repair factor IIH helicase subunit XPB; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 89.404734 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG TKVDEYGAKD YRLQMPLKDD HTSRPLWVAP DGHIFLEAF SPVYKYAQDF LVAIAEPVCR PTHVHEYKLT AYSLYAAVSV GLQTSDITEY LRKLSKTGVP DGIMQFIKLC T VSYGKVKL VLKHNRYFVE SCHPDVIQHL LQDPVIRECR LRNSEGEATE LITETFTSKS AISKTAESSG GPSTSRVTDP QG KSDIPMD LFDFYEQMDK DEEEEEETQT VSFEVKQEMI EELQKRCIHL EYPLLAEYDF RNDSVNPDIN IDLKPTAVLR PYQ EKSLRK MFGNGRARSG VIVLPCGAGK SLVGVTAACT VRKRCLVLGN SAVSVEQWKA QFKMWSTIDD SQICRFTSDA KDKP IGCSV AISTYSMLGH TTKRSWEAER VMEWLKTQEW GLMILDEVHT IPAKMFRRVL TIVQAHCKLG LTATLVREDD KIVDL NFLI GPKLYEANWM ELQNNGYIAK VQCAEVWCPM SPEFYREYVA IKTKKRILLY TMNPNKFRAC QFLIKFHERR NDKIIV FAD NVFALKEYAI RLNKPYIYGP TSQGERMQIL QNFKHNPKIN TIFISKVGDT SFDLPEANVL IQISSHGGSR RQEAQRL GR VLRAKKGMVA EEYNAFFYSL VSQDTQEMAY STKRQRFLVD QGYSFKVITK LAGMEEEDLA FSTKEEQQQL LQKVLAAT D LDAEEEVVAG EFGSRSSQAS RRFGTMSSMS GADDTVYMEY HSSRSKAPSK HVHPLFKRFR K

Macromolecule #9: Cyclin-dependent kinase 7

• Macromolecule: Name: Cyclin-dependent kinase 7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: cyclin-dependent kinase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.090344 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MALDVKSRAK RYEKLDFLGE GQFATVYKAR DKNTNQIVAI KKIKLGHRSE AKDGINRTAL REIKLLQELS HPNIIGLLDA FGHKSNISL VFDFMETDLE VIIKDNSLVL TPSHIKAYML MTLQGLEYLH QHWILHRDLK PNNLLLDENG VLKLADFGLA K SFGSPNRA YTHQVVTRWY RAPELLFGAR MYGVGVDMWA VGCILAELLL RVPFLPGDSD LDQLTRIFET LGTPTEEQWP DM CSLPDYV TFKSFPGIPL HHIFSAAGDD LLDLIQGLFL FNPCARITAT QALKMKYFSN RPGPTPGCQL PRPNCPVETL KEQ SNPALA IKRKRTEALE QGGLPKKLIF

Macromolecule #10: Cyclin-H

• Macromolecule: Name: Cyclin-H / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.695473 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKR FYLNNSVMEY HPRIIMLTCA FLACKVDEFN VSSPQFVGNL RESPLGQEKA LEQILEYELL LIQQLNFHLI V HNPYRPFE GFLIDLKTRY PILENPEILR KTADDFLNRI ALTDAYLLYT PSQIALTAIL SSASRAGITM ESYLSESLML KE NRTCLSQ LLDIMKSMRN LVKKYEPPRS EEVAVLKQKL ERCHSAELAL NVITKKRKGY EDDDYVSKKS KHEEEEWTDD DLV ESL

Macromolecule #11: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 217.450078 KDa

Sequence

String:

MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ PEGDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EP RYARPEW MIVTVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDN ELPGLP RAMQKSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNID RLQEL VRRGNSQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWS TFRL NLSVTTPYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEV MNL LMFLSTWDGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGIL CK KSLGTSAGSL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKA H NNELEPTPGN TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPF GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQL RYGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM R EDREVLRV IFPTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LL FNIHLRS TLCSRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLG VPRLKE LINISKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMP DFDVA RISPWLLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMD DDVF LRCIESNMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSN DIV EIFTVLGIEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLM EA AAHGESDPMK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPW N QGATPAYGAW SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAY EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPNYS PTSPNYTPTS P SYSPTSPS YSPTSPNYTP TSPNYSPTSP SYSPTSPSYS PTSPSYSPSS PRYTPQSPTY TPSSPSYSPS SPSYSPTSPK YT PTSPSYS PSSPEYTPTS PKYSPTSPKY SPTSPKYSPT SPTYSPTTPK YSPTSPTYSP TSPVYTPTSP KYSPTSPTYS PTS PKYSPT SPTYSPTSPK GSTYSPTSPG YSPTSPTYSL TSPAISPDDS DEEN

Macromolecule #12: DNA-directed RNA polymerase subunit beta

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 134.041422 KDa

Sequence

String:

MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL EGTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

Macromolecule #13: DNA-directed RNA polymerase II subunit RPB3

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 31.439074 KDa

Sequence

String:

MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN

Macromolecule #14: RPOL4c domain-containing protein

• Macromolecule: Name: RPOL4c domain-containing protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 16.331255 KDa

Sequence

String:

MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQ KKLHKFELAC LANLCPETAE ESKALIPSLE GRFEDEELQQ ILDDIQTKRS FQY

Macromolecule #15: DNA-directed RNA polymerase II subunit E

• Macromolecule: Name: DNA-directed RNA polymerase II subunit E / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 24.644318 KDa

Sequence

String:

MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

Macromolecule #16: DNA-directed RNA polymerase II subunit F

• Macromolecule: Name: DNA-directed RNA polymerase II subunit F / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 14.477001 KDa

Sequence

String:

MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIISD

Macromolecule #17: DNA-directed RNA polymerase II subunit RPB7

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 19.314283 KDa

Sequence

String:

MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

Macromolecule #18: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 17.162273 KDa

Sequence

String:

MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

Macromolecule #19: DNA-directed RNA polymerase II subunit RPB9

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 14.541221 KDa

Sequence

String:

MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

Macromolecule #20: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 7.655123 KDa

Sequence

String:

MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

Macromolecule #21: RNA_pol_L_2 domain-containing protein

• Macromolecule: Name: RNA_pol_L_2 domain-containing protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 13.310284 KDa

Sequence

String:

MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

Macromolecule #22: RNA polymerase II subunit K

• Macromolecule: Name: RNA polymerase II subunit K / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 7.018244 KDa

Sequence

String:

MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

Macromolecule #23: Transcription initiation factor IIB

• Macromolecule: Name: Transcription initiation factor IIB / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO / EC number: histone acetyltransferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.877949 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTG AASFDEFGNS KYQNRRTMSS SDRAMMNAFK EITTMADRIN LPRNIVDRTN NLFKQVYEQK SLKGRANDAI A SACLYIAC RQEGVPRTFK EICAVSRISK KEIGRCFKLI LKALETSVDL ITTGDFMSRF CSNLCLPKQV QMAATHIARK AV ELDLVPG RSPISVAAAA IYMASQASAE KRTQKEIGDI AGVADVTIRQ SYRLIYPRAP DLFPTDFKFD TPVDKLPQL

Macromolecule #25: TATA-box-binding protein

• Macromolecule: Name: TATA-box-binding protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.729938 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI VSTVNLGCKL DLKTIALRAR NAEYNPKRFA AVIMRIREPR TTALIFSSGK MVCTGAKSEE QSRLAARKYA RV VQKLGFP AKFLDFKIQN MVGSCDVKFP IRLEGLVLTH QQFSSYEPEL FPGLIYRMIK PRIVLLIFVS GKVVLTGAKV RAE IYEAFE NIYPILKGFR KTT

Macromolecule #26: General transcription factor IIF subunit 1

• Macromolecule: Name: General transcription factor IIF subunit 1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 58.343578 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLK EFRPEDQPWL LRVNGKSGRK FKGIKKGGVT ENTSYYIFTQ CPDGAFEAFP VHNWYNFTPL ARHRTLTAEE A EEEWERRN KVLNHFSIMQ QRRLKDQDQD EDEEEKEKRG RRKASELRIH DLEDDLEMSS DASDASGEEG GRVPKAKKKA PL AKGGRKK KKKKGSDDEA FEDSDDGDFE GQEVDYMSDG SSSSQEEPES KAKAPQQEEG PKGVDEQSDS SEESEEEKPP EED KEEEEE KKAPTPQEKK RRKDSSEESD SSEESDIDSE ASSALFMAKK KTPPKRERKP SGGSSRGNSR PGTPSAEGGS TSST LRAAA SKLEQGKRVS EMPAAKRLRL DTGPQSLSGK STPQPPSGKT TPNSGDVQVT EDAVRRYLTR KPMTTKDLLK KFQTK KTGL SSEQTVNVLA QILKRLNPER KMINDKMHFS LKE

Macromolecule #27: General transcription factor IIF subunit 2

• Macromolecule: Name: General transcription factor IIF subunit 2 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.427309 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVG GQTLTVFTES SSDKLSLEGI VVQRAECRPA ASENYMRLKR LQIEESSKPV RLSQQLDKVV TTNYKPVANH Q YNIEYERK KKEDGKRARA DKQHVLDMLF SAFEKHQYYN LKDLVDITKQ PVVYLKEILK EIGVQNVKGI HKNTWELKPE YR HYQGEEK SD

Macromolecule #29: Transcription initiation factor IIA subunit 1

• Macromolecule: Name: Transcription initiation factor IIA subunit 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 41.544551 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQ AQPQQTVPQQ AQTQQVLIPA SQQATAPQVI VPDSKLIQHM NASNMSAAAT AATLALPAGV TPVQQILTNS G QLLQVVRA ANGAQYIFQP QQSVVLQQQV IPQMQPGGVQ APVIQQVLAP LPGGISPQTG VIIQPQQILF TGNKTQVIPT TV AAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVDGTG DTSSEEDEDE EEDYDDDEEE DKEKDGAEDG QVEEEPLNSE DDV SDEEGQ ELFDTENVVV CQYDKIHRSK NKWKFHLKDG IMNLNGRDYI FSKAIGDAEW

Macromolecule #30: Transcription initiation factor IIA subunit 2

• Macromolecule: Name: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.469091 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG SLNTYRFCDN VWTFVLNDVE FREVTELIK VDKVKIVACD GKNTGSNTTE

Macromolecule #31: General transcription factor IIE subunit 1

• Macromolecule: Name: General transcription factor IIE subunit 1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.516094 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRH NYYFINYRTL VNVVKYKLDH MRRRIETDER DSTNRASFKC PVCSSTFTDL EANQLFDPMT GTFRCTFCHT E VEEDESAM PKKDARTLLA RFNEQIEPIY ALLRETEDVN LAYEILEPEP TEIPALKQSK DHAATTAGAA SLAGGHHREA WA TKGPSYE DLYTQNVVIN MDDQEDLHRA SLEGKSAKER PIWLRESTVQ GAYGSEDMKE GGIDMDAFQE REEGHAGPDD NEE VMRALL IHEKKTSSAM AGSVGAAAPV TAANGSDSES ETSESDDDSP PRPAAVAVHK REEDEEEDDE FEEVADDPIV MVAG RPFSY SEVSQRPELV AQMTPEEKEA YIAMGQRMFE DLFE

Macromolecule #32: Transcription initiation factor IIE subunit beta

• Macromolecule: Name: Transcription initiation factor IIE subunit beta / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.106824 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRH QRGDTHPLTL DEILDETQHL DIGLKQKQWL MTEALVNNPK IEVIDGKYAF KPKYNVRDKK ALLRLLDQHD Q RGLGGILL EDIEEALPNS QKAVKALGDQ ILFVNRPDKK KILFFNDKSC QFSVDEEFQK LWRSVTVDSM DEEKIEEYLK RQ GISSMQE SGPKKVAPIQ RRKKPASQKK RRFKTHNEHL AGVLKDYSDI TSSK

Macromolecule #33: Unassigned peptide, likely TFIIE-beta

• Macromolecule: Name: Unassigned peptide, likely TFIIE-beta / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.635006 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #34: Unassigned peptide, likely XPB

• Macromolecule: Name: Unassigned peptide, likely XPB / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 698.854 Da
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #35: Mediator of RNA polymerase II transcription subunit 6

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.464627 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQDKVR P KAKRKEEP SSIFQRQRVD ALLLDLRQKF PPKFVQLKPG EKPVPVDQTK KEAEPIPETV KPEEKETTKN VQQTVSAKGP PE KRMRLQ

Macromolecule #36: Mediator of RNA polymerase II transcription subunit 8

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.112906 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP NKQTFNPTDT NALVAAVAFG KGLSNWRPSG SSGPGQAGQP GAGTILAGTS GLQQVQMAGA PSQQQPMLSG VQ MAQAGQP GKMPSGIKTN IKSASMHPYQ R

Macromolecule #37: Mediator of RNA polymerase II transcription subunit 11

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 11; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.145829 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MATYSLANER LRALEDIERE IGAILQNAGT VILELSKEKT NERLLDRQAA AFTASVQHVE AELSAQIRYL TQVATGQPHE GSSYSSRKD CQMALKRVDY ARLKLSDVAR TCEQMLEN

Macromolecule #38: Mediator of RNA polymerase II transcription subunit 17

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 17; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 72.984047 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MSGVRAVRIS IESACEKQVH EVGLDGTETY LPPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGTEGDAQEW PGAGSSADQD DEEGVVKFQ PSLWPWDSVR NNLRSALTEM CVLYDVLSIV RDKKFMTLDP VSQDALPPKQ NPQTLQLISK KKSLAGAAQI L LKGAERLT KSVTENQENK LQRDFNSELL RLRQHWKLRK VGDKILGDLS YRSAGSLFPH HGTFEVIKNT DLDLDKKIPE DY CPLDVQI PSDLEGSAYI KVSIQKQAPD IGDLGTVNLF KRPLPKSKPG SPHWQTKLEA AQNVLLCKEI FAQLSREAVQ IKS QVPHIV VKNQIISQPF PSLQLSISLC HSSNDKKSQK FATEKQCPED HLYVLEHNLH LLIREFHKQT LSSIMMPHPA SAPF GHKRM RLSGPQAFDK NEINSLQSSE GLLEKIIKQA KHIFLRSRAA ATIDSLASRI EDPQIQAHWS NINDVYESSV KVLIT SQGY EQICKSIQLQ LNIGVEQIRV VHRDGRVITL SYQEQELQDF LLSQMSQHQV HAVQQLAKVM GWQVLSFSNH VGLGPI ESI GNASAITVAS PSGDYAISVR NGPESGSKIM VQFPRNQCKD LPKSDVLQDN KWSHLRGPFK EVQWNKMEGR NFVYKME LL MSALSPCLL

Macromolecule #39: Mediator of RNA polymerase II transcription subunit 18

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 18; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.69373 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKIMVYKIF RILVPGNTDS T EALSLSYL VELSVVAPAG QDMVSDDMKN FAEQLKPLVH LEKIDPKRLM

Macromolecule #40: Mediator of RNA polymerase II transcription subunit 20

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 20; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.243762 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH TPGAPAVFGN RHDAVYGPAD TMVQYMELFN KIRKQQQVPV AGIR

Macromolecule #41: Mediator of RNA polymerase II transcription subunit 22

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 22; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.243713 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRTLQ EECDRKLITL RDEISIDLYE LEEEYYSSSS SLCEANDLPL CEAYGRLDLD T DSADGLSA PLLASPEPSA GPLQVAAPAH SHAGGPGPTE HA

Macromolecule #42: Mediator of RNA polymerase II transcription subunit 4

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.781424 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKI HHEMQVLEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV PGDPRRPYPT DLEMRSGLLG QMNNPSTNGV NGHLPGDALA AGRLPDVLAP QYPWQSNDMS MNMLPPNHSS DF LLEPPGH NKENEDDVEI MSTDSSSSSS ESD

Macromolecule #43: Mediator of RNA polymerase II transcription subunit 7

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.285281 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ASLPDDLPHS EAGMRVKTEP MDADDSNNCT GQNEHQRENS GHRRDQIIEK DAALCVLIDE MNERP

Macromolecule #44: Mediator of RNA polymerase II transcription subunit 9

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.42275 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNA LKSKFQEMRK LISTMPGIHL SPEQQQQQLQ SLREQVRTKN ELLQKYKSLC MFEIPKE

Macromolecule #45: Mediator of RNA polymerase II transcription subunit 10

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 10; type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.710969 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLNQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERAL AKNEQVKGKI DTMKKFKSLL IQELSKVFPE DMAKYRSIRG EDHPPS

Macromolecule #46: Mediator of RNA polymerase II transcription subunit 14

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 14; type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 160.787594 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFA SRTRQLFVRL LALVKWANNA GKVEKCAMIS SFLDQQAILF VDTADRLASL ARDALVHARL PSFAIPYAID V LTTGSYPR LPTCIRDKII PPDPITKIEK QATLHQLNQI LRHRLVTTDL PPQLANLTVA NGRVKFRVEG EFEATLTVMG DD PDVPWRL LKLEILVEDK ETGDGRALVH SMQISFIHQL VQSRLFADEK PLQDMYNCLH SFCLSLQLEV LHSQTLMLIR ERW GDLVQV ERYHAGKCLS LSVWNQQVLG RKTGTASVHK VTIKIDENDV SKPLQIFHDP PLPASDSKLV ERAMKIDHLS IEKL LIDSV HARAHQKLQE LKAILRGFNA NENSSIETAL PALVVPILEP CGNSECLHIF VDLHSGMFQL MLYGLDQATL DDMEK SVND DMKRIIPWIQ QLKFWLGQQR CKQSIKHLPT ISSETLQLSN YSTHPIGNLS KNKLFIKLTR LPQYYIVVEM LEVPNK PTQ LSYKYYFMSV NAADREDSPA MALLLQQFKE NIQDLVFRTK TGKQTRTNAK RKLSDDPCPV ESKKTKRAGE MCAFNKV LA HFVAMCDTNM PFVGLRLELS NLEIPHQGVQ VEGDGFSHAI RLLKIPPCKG ITEETQKALD RSLLDCTFRL QGRNNRTW V AELVFANCPL NGTSTREQGP SRHVYLTYEN LLSEPVGGRK VVEMFLNDWN SIARLYECVL EFARSLPDIP AHLNIFSEV RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP NSGCSNCHNT ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVP MLGLTQRTNT AYQCFSILPQ SSTHIRLAFR NMYCIDIYCR SRGVVAIRDG AYSLFDNSKL VEGFYPAPGL K TFLNMFVD SNQDARRRSV NEDDNPPSPI GGDMMDSLIS QLQPPPQQQP FPKQPGTSGA YPLTSPPTSY HSTVNQSPSM MH TQSPGNL HAASSPSGAL RAPSPASFVP TPPPSSHGIS IGPGASFASP HGTLDPSSPY TMVSPSGRAG NWPGSPQVSG PSP AARMPG MSPANPSLHS PVPDASHSPR AGTSSQTMPT NMPPPRKLPQ RSWAASIPTI LTHSALNILL LPSPTPGLVP GLAG SYLCS PLERFLGSVI MRRHLQRIIQ QETLQLINSN EPGVIMFKTD ALKCRVALSP KTNQTLQLKV TPENAGQWKP DELQV LEKF FETRVAGPPF KANTLIAFTK LLGAPTHILR DCVHIMKLEL FPDQATQLKW NVQFCLTIPP SAPPIAPPGT PAVVLK SKM LFFLQLTQKT SVPPQEPVSI IVPIIYDMAS GTTQQADIPR QQNSSVAAPM MVSNILKRFA EMNPPRQGEC TIFAAVR DL MANLTLPPGG RP

Macromolecule #47: Mediator of RNA polymerase II transcription subunit 19

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 19; type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.321146 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILSS SFNPITGTML AGFRLHTGPL P EQCRLMHI QPPKKKNKHK HKQSRTQDPV PPETPSDSDH KKKKKKKEED PDRKRKKKEK KKKKNRHSPD HPGMGSSQAS SS SSLR

Macromolecule #48: Mediator of RNA polymerase II transcription subunit 21

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 21; type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.575234 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAAS LYKLEEENHE AATCLEDVVY RGDMLLEKIQ SALADIAQSQ LKTRSGTHSQ SLPDS

Macromolecule #49: Mediator of RNA polymerase II transcription subunit 31

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 31; type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.826116 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELV NAQCAKFIDE QQILHWQHYS RKRMRLQQAL AEQQQQNNTS GK

Macromolecule #50: Mediator of RNA polymerase II transcription subunit 27

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 27; type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.478742 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR IDRMFPEMSI HLSRPNGTSA MLLVTLGKVL KVIVVMRSLF IDRTIVKGYN ENVYTEDGKL DIWSKSNYQV FQ KVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

Macromolecule #51: Mediator of RNA polymerase II transcription subunit 28

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 28; type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.540107 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIAR QTECFFLQKR LQLSVQKPEQ VIKEDVSELR NELQRKDALV QKHLTKLRHW QQVLEDINVQ HKKPADIPQG S LAYLEQAS ANIPAPLKPT

Macromolecule #52: unassigned peptide (MED29 or MED30)

• Macromolecule: Name: unassigned peptide (MED29 or MED30) / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.720111 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

Macromolecule #53: Mediator of RNA polymerase II transcription subunit 30

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 30; type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.301229 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLF RKLRLVYDKC NENCGGMDPI PVEQLIPYVE EDGSKNDDRA GPPRFASEER REIAEVNKKL KQKNQQLKQI M DQLRNLIW DINAMLAMRN

Macromolecule #54: unassigned peptide (MED14)

• Macromolecule: Name: unassigned peptide (MED14) / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.890321 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #55: unassigned peptide (MED6)

• Macromolecule: Name: unassigned peptide (MED6) / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.379692 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #56: unassigned peptide (MED17)

• Macromolecule: Name: unassigned peptide (MED17) / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 954.168 Da
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #57: unassigned peptide (MED29 or MED30)

• Macromolecule: Name: unassigned peptide (MED29 or MED30) / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trichoplusia ni (cabbage looper)
• Molecular weight: Theoretical: 1.549902 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #58: unassigned peptide (MED29 or MED30)

• Macromolecule: Name: unassigned peptide (MED29 or MED30) / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trichoplusia ni (cabbage looper)
• Molecular weight: Theoretical: 1.975426 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #24: NT

• Macromolecule: Name: NT / type: dna / ID: 24 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: Human mastadenovirus C
• Molecular weight: Theoretical: 32.911859 KDa

Sequence

String:

(DC)(DG)(DG)(DG)(DT)(DG)(DT)(DT)(DC)(DC) (DT)(DG)(DA)(DA)(DG)(DG)(DG)(DG)(DG)(DG) (DC)(DT)(DA)(DT)(DA)(DA)(DA)(DA)(DG) (DG)(DG)(DG)(DG)(DT)(DG)(DG)(DG)(DG)(DG) (DC) (DG)(DC)(DG)(DT)(DT)(DC)(DG)(DT) (DC)(DC)(DT)(DC)(DA)(DC)(DT)(DC)(DT)(DC) (DT)(DT) (DC)(DC)(DG)(DC)(DA)(DT)(DC) (DG)(DC)(DT)(DG)(DT)(DC)(DT)(DG)(DC)(DG) (DA)(DG)(DG) (DG)(DC)(DC)(DA)(DG)(DC) (DT)(DG)(DT)(DT)(DG)(DG)(DG)(DG)(DT)(DG) (DA)(DG)(DT)(DA) (DC)(DT)(DC)(DC)(DC) (DT)

Macromolecule #28: TEMPLATE

• Macromolecule: Name: TEMPLATE / type: dna / ID: 28 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: Human mastadenovirus C
• Molecular weight: Theoretical: 32.508752 KDa

Sequence

String:

(DA)(DG)(DG)(DG)(DA)(DG)(DT)(DA)(DC)(DT) (DC)(DA)(DC)(DC)(DC)(DC)(DA)(DA)(DC)(DA) (DG)(DC)(DT)(DG)(DG)(DC)(DC)(DC)(DT) (DC)(DG)(DC)(DA)(DG)(DA)(DC)(DA)(DG)(DC) (DG) (DA)(DT)(DG)(DC)(DG)(DG)(DA)(DA) (DG)(DA)(DG)(DA)(DG)(DT)(DG)(DA)(DG)(DG) (DA)(DC) (DG)(DA)(DA)(DC)(DG)(DC)(DG) (DC)(DC)(DC)(DC)(DC)(DA)(DC)(DC)(DC)(DC) (DC)(DT)(DT) (DT)(DT)(DA)(DT)(DA)(DG) (DC)(DC)(DC)(DC)(DC)(DC)(DT)(DT)(DC)(DA) (DG)(DG)(DA)(DA) (DC)(DA)(DC)(DC)(DC) (DG)

Macromolecule #59: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 59 / Number of copies: 1 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Macromolecule #60: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 60 / Number of copies: 18 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #61: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 61 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 58.56 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 25967