3-hydroxyisobutyryl-CoA hydrolase activity / ATPase inhibitor activity / mitochondrial genome maintenance / ribonuclease III activity / negative regulation of ATP-dependent activity / DNA strand exchange activity / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation ...3-hydroxyisobutyryl-CoA hydrolase activity / ATPase inhibitor activity / mitochondrial genome maintenance / ribonuclease III activity / negative regulation of ATP-dependent activity / DNA strand exchange activity / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / superoxide dismutase activity / RNA processing / rRNA processing / large ribosomal subunit / double-stranded RNA binding / single-stranded DNA binding / small ribosomal subunit / transferase activity / cytosolic small ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mitochondrion / RNA binding / metal ion binding / cytoplasm 類似検索 - 分子機能
54S ribosomal protein L44, mitochondrial / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain / Transcriptional regulation of mitochondrial recombination / Mitochondrial ribosomal protein mL59 ...54S ribosomal protein L44, mitochondrial / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain / Transcriptional regulation of mitochondrial recombination / Mitochondrial ribosomal protein mL59 / 54S ribosomal protein L28, mitochondrial / Ribosomal protein L31, mitochondrial / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein L31 / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial / Ribosomal protein S35, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / IGR protein motif / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / Ribonuclease-III-like / IGR protein motif / IGR / Ribonuclease III / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / 28S ribosomal protein S25, mitochondrial / Ribonuclease III family domain profile. / Ribonuclease III family / Ribonuclease III domain / Ribosomal protein L37, mitochondrial / Mitochondrial ribosomal protein L37 / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Ribosomal protein L49/IMG2 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial large subunit ribosomal protein (Img2) / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / Double-stranded RNA binding motif / Ribonuclease III, endonuclease domain superfamily / Double-stranded RNA-binding domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / ClpP/crotonase-like domain superfamily / Ribosomal protein L1, 3-layer alpha/beta-sandwich / NUDIX hydrolase-like domain superfamily / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L11, bacterial-type / Sterile alpha motif/pointed domain superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. 類似検索 - ドメイン・相同性
ADENOSINE-5'-TRIPHOSPHATE / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SPERMINE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Mitochondrial 37S ribosomal protein MRP2 / ATP synthase F1 subunit epsilon ...ADENOSINE-5'-TRIPHOSPHATE / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SPERMINE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Mitochondrial 37S ribosomal protein MRP2 / ATP synthase F1 subunit epsilon / Nucleic acid-binding protein / 60S ribosomal protein L2, mitochondrial / 37S ribosomal protein mrp5 / RIBOSOMAL_L9 domain-containing protein / Ribosomal protein S16 / Ribosomal protein L15 / Manganese and iron superoxide dismutase / Large ribosomal subunit protein mL59 domain-containing protein / Mitochondrial 54S ribosomal protein YmL19 / 54S ribosomal protein L24 / Ribosomal protein S6 / Ribonuclease III / KOW domain-containing protein / Ribosomal protein S12 / Translational machinery component / Ribosomal_L6 domain-containing protein / Ribosomal protein S19/S15 / Mitochondrial ribosomal protein subunit L23 / Ribosomal protein S21 / MRP-L46 domain-containing protein / Mitochondrial 37S ribosomal protein S27 / L51_S25_CI-B8 domain-containing protein / 54S ribosomal protein L4, mitochondrial / Uncharacterized protein / 54S ribosomal protein IMG1, mitochondrial / S4 domain-containing protein / Protein FYV4, mitochondrial / PEBP-like protein / Mitochondrial ribosomal protein L37-domain-containing protein / 37S ribosomal protein mrp10, mitochondrial / Ribosomal protein L13 / 54S ribosomal protein L27, mitochondrial / RNase III domain-containing protein / Ribosomal protein L33 / Ribosomal protein L14 / Ribosomal_S7 domain-containing protein / 30S ribosomal protein S10, mitochondrial / Mitochondrial large ribosomal subunit protein / Ribosomal protein L17 / 37S ribosomal protein S9, mitochondrial / 37S ribosomal protein S8, mitochondrial / 54S ribosomal protein L31, mitochondrial / Ribosomal protein S5 / Large ribosomal subunit protein bL32m / Related to ribosomal protein YmL11, mitochondrial / Related to ribosomal protein YmL49, mitochondrial / Probable ribosomal protein YmL44, mitochondrial / Probable ribosomal protein S13 / Large ribosomal subunit protein mL61 / Small ribosomal subunit protein mS27 / Large ribosomal subunit protein bL36m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein mL50 / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS45 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS26 / 60S ribosomal protein L1, mitochondrial / Ribosomal protein S18 / Related to ribosomal protein L3, mitochondrial / Small ribosomal subunit protein mS23 / Related to ribosomal protein YmL20, mitochondrial / Related to ribosomal protein S5 (Mitochondrial) / Related to ribosomal protein S15 (Mitochondrial) / Related to ribosomal protein L16, mitochondrial / Related to ribosomal protein L30 / Related to 60s ribosomal protein L2 (Mitochondrial) / Related to ribosomal protein L5, mitochondrial / Related to ribosomal protein L34, mitochondrial / ClpP/crotonase / Related to ribosomal protein YmL36, mitochondrial / Protein FYV4, mitochondrial / Related to ribosomal protein YmL32 (Mitochondrial) / 37S ribosomal protein S24, mitochondrial / Small ribosomal subunit protein uS2m / Large ribosomal subunit protein uL4m 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi. 著者: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts / 要旨: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.
A: 23S rRNA e: 60S ribosomal protein L1, mitochondrial B: 60S ribosomal protein L2, mitochondrial C: Related to ribosomal protein L3, mitochondrial D: 60S ribosomal protein L4, variant E: Related to ribosomal protein L5, mitochondrial F: uL6m G: RIBOSOMAL_L9 domain-containing protein f: Related to ribosomal protein YmL11, mitochondrial g: uL11m H: uL13m I: Ribosomal protein L14 J: Ribosomal protein L15 K: Related to ribosomal protein L16, mitochondrial L: uL17m M: bL19m N: Related to ribosomal protein YmL49, mitochondrial O: Mitochondrial large ribosomal subunit P: uL23m Q: KOW domain-containing protein R: Related to 60s ribosomal protein L2 (Mitochondrial) S: bL28m T: 54S ribosomal protein L4, mitochondrial U: Related to ribosomal protein L30 V: Related to ribosomal protein YmL36, mitochondrial W: Related to ribosomal protein YmL32 (Mitochondrial),Mitochondrial ribosomal protein subunit L32 X: bL33m Y: Related to ribosomal protein L34, mitochondrial 0: Ribosomal protein 1: PEBP-like protein 2: mL40 3: mL41 4: L51_S25_CI-B8 domain-containing protein 5: Ribonuclease III 6: MRP-L46 domain-containing protein 7: mL49 8: mL50 h: Probable ribosomal protein YmL44, mitochondrial i: mL54 9: RNase III domain-containing protein a: Related to ribosomal protein YmL20, mitochondrial b: Mitoc_mL59 domain-containing protein c: 54S ribosomal protein L31, mitochondrial d: mL67 j: L51_S25_CI-B8 domain-containing protein bb: tRNA P/E state AA: bS1m BB: Mito ribosomal protein S2 CC: Ribosomal protein S5 DD: S4 RNA-binding domain-containing protein EE: Related to ribosomal protein S5 (Mitochondrial) FF: Ribosomal protein S6 GG: Ribosomal_S7 domain-containing protein HH: uS8m II: uS9m JJ: 30S ribosomal protein S10, mitochondrial KK: Translational machinery component LL: Ribosomal protein S12 MM: Probable ribosomal protein S13 NN: Mitochondrial 37S ribosomal protein MRP2 OO: Related to ribosomal protein S15 (Mitochondrial) PP: bS16m QQ: uS17m RR: Ribosomal protein S18 SS: Ribosomal protein S19/S15 TT: bS21m UU: 37S ribosomal protein S25, mitochondrial VV: mS26 WW: mS27 XX: Mitochondrial ribosomal protein DAP3 YY: mS33 ZZ: 37S ribosomal protein S24, mitochondrial 11: 37S ribosomal protein mrp10, mitochondrial 22: DUF1713 domain-containing protein 33: Protein FYV4, mitochondrial 44: Manganese and iron superoxide dismutase 55: Manganese and iron superoxide dismutase 66: mS45 77: mS46 88: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial 00: IF1 99: IF1 aa: 16S rRNA ee: mRNA ヘテロ分子