- PDB-6vwi: Head region of the closed conformation of the human type 1 insuli... -
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基本情報
登録情報
データベース: PDB / ID: 6vwi
タイトル
Head region of the closed conformation of the human type 1 insulin-like growth factor receptor ectodomain in complex with human insulin-like growth factor II.
要素
Insulin-like growth factor II
Leucine-zippered human type 1 insulin-like growth factor receptor ectodomain
キーワード
SIGNALING PROTEIN / Type 1 insulin-like growth factor receptor / Insulin-like growth factor II / ectodomain receptor / tyrosine kinase
機能・相同性
機能・相同性情報
spongiotrophoblast cell proliferation / cardiac atrium development / positive regulation of skeletal muscle tissue growth / negative regulation of cholangiocyte apoptotic process / negative regulation of muscle cell differentiation / embryonic placenta morphogenesis / regulation of muscle cell differentiation / insulin-like growth factor receptor activity / positive regulation of steroid hormone biosynthetic process / protein kinase complex ...spongiotrophoblast cell proliferation / cardiac atrium development / positive regulation of skeletal muscle tissue growth / negative regulation of cholangiocyte apoptotic process / negative regulation of muscle cell differentiation / embryonic placenta morphogenesis / regulation of muscle cell differentiation / insulin-like growth factor receptor activity / positive regulation of steroid hormone biosynthetic process / protein kinase complex / Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) / protein transporter activity / IRS-related events triggered by IGF1R / insulin-like growth factor binding / genomic imprinting / negative regulation of muscle cell apoptotic process / cellular response to progesterone stimulus / positive regulation of DNA metabolic process / positive regulation of organ growth / protein localization to nuclear periphery / FCERI mediated MAPK activation / cellular response to zinc ion starvation / cellular response to aldosterone / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / exocrine pancreas development / insulin receptor complex / cellular response to testosterone stimulus / negative regulation of hepatocyte apoptotic process / insulin-like growth factor I binding / positive regulation of multicellular organism growth / insulin receptor activity / transcytosis / nitrogen catabolite activation of transcription from RNA polymerase II promoter / alphav-beta3 integrin-IGF-1-IGF1R complex / response to alkaloid / Oxidative Stress Induced Senescence / cellular response to angiotensin / positive regulation of protein-containing complex disassembly / positive regulation of vascular endothelial cell proliferation / dendritic spine maintenance / cellular response to insulin-like growth factor stimulus / response to L-glutamate / insulin binding / negative regulation of MAPK cascade / establishment of cell polarity / transmembrane receptor protein tyrosine kinase activator activity / positive regulation of activated T cell proliferation / positive regulation of axon regeneration / amyloid-beta clearance / positive regulation of osteoblast proliferation / positive regulation of cytokinesis / TFIID-class transcription factor complex binding / Respiratory syncytial virus (RSV) attachment and entry / regulation of JNK cascade / positive regulation of cell division / insulin receptor substrate binding / estrous cycle / positive regulation of glycogen biosynthetic process / G-protein alpha-subunit binding / response to vitamin E / embryonic placenta development / positive regulation of transcription initiation by RNA polymerase II / SHC-related events triggered by IGF1R / positive regulation of RNA polymerase II transcription preinitiation complex assembly / phosphatidylinositol 3-kinase binding / amino acid biosynthetic process / positive regulation of insulin receptor signaling pathway / peptidyl-tyrosine autophosphorylation / cellular response to transforming growth factor beta stimulus / striated muscle cell differentiation / T-tubule / cellular response to amino acid starvation / protein serine/threonine kinase activator activity / positive regulation of mitotic nuclear division / cellular response to dexamethasone stimulus / cerebellum development / axonogenesis / phosphatidylinositol 3-kinase/protein kinase B signal transduction / insulin-like growth factor receptor signaling pathway / platelet alpha granule lumen / caveola / cellular response to estradiol stimulus / hippocampus development / cellular response to glucose stimulus / animal organ morphogenesis / positive regulation of smooth muscle cell proliferation / insulin-like growth factor receptor binding / growth factor activity / response to nicotine / insulin receptor binding / hormone activity / receptor protein-tyrosine kinase / cellular response to mechanical stimulus / osteoblast differentiation / RNA polymerase II transcription regulator complex / cellular response to amyloid-beta 類似検索 - 分子機能
Insulin-like growth factor II E-peptide, C-terminal / Insulin-like growth factor II / Insulin-like growth factor II E-peptide / Insulin-like, subunit E / Insulin-like / Insulin-like growth factor / Tyrosine-protein kinase, insulin-like receptor / Basic region leucine zipper / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. ...Insulin-like growth factor II E-peptide, C-terminal / Insulin-like growth factor II / Insulin-like growth factor II E-peptide / Insulin-like, subunit E / Insulin-like / Insulin-like growth factor / Tyrosine-protein kinase, insulin-like receptor / Basic region leucine zipper / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain superfamily / Basic-leucine zipper domain / Tyrosine-protein kinase, receptor class II, conserved site / Receptor tyrosine kinase class II signature. / Insulin family / Insulin/IGF/Relaxin family / Insulin, conserved site / Insulin family signature. / Insulin-like / Insulin / insulin-like growth factor / relaxin family. / Insulin-like superfamily / Receptor L-domain / Furin-like cysteine-rich domain / Receptor L-domain superfamily / Furin-like cysteine rich region / Receptor L domain / Furin-like repeat / Furin-like repeats / Growth factor receptor cysteine-rich domain superfamily / Fibronectin type III domain / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Protein tyrosine and serine/threonine kinase / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Immunoglobulin-like fold / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
Insulin-like growth factor II / General control transcription factor GCN4 / Insulin-like growth factor 1 receptor 類似検索 - 構成要素
National Health and Medical Research Council (NHMRC, Australia)
APP1128553
オーストラリア
Wellcome Trust
209407/Z/17/
英国
引用
ジャーナル: Structure / 年: 2020 タイトル: How IGF-II Binds to the Human Type 1 Insulin-like Growth Factor Receptor. 著者: Yibin Xu / Nicholas S Kirk / Hariprasad Venugopal / Mai B Margetts / Tristan I Croll / Jarrod J Sandow / Andrew I Webb / Carlie A Delaine / Briony E Forbes / Michael C Lawrence / 要旨: Human type 1 insulin-like growth factor receptor (IGF-1R) signals chiefly in response to the binding of insulin-like growth factor I. Relatively little is known about the role of insulin-like growth ...Human type 1 insulin-like growth factor receptor (IGF-1R) signals chiefly in response to the binding of insulin-like growth factor I. Relatively little is known about the role of insulin-like growth factor II signaling via IGF-1R, despite the affinity of insulin-like growth factor II for IGF-1R being within an order of magnitude of that of insulin-like growth factor I. Here, we describe the cryoelectron microscopy structure of insulin-like growth factor II bound to a leucine-zipper-stabilized IGF-1R ectodomain, determined in two conformations to a maximum average resolution of 3.2 Å. The two conformations differ in the relative separation of their respective points of membrane entry, and comparison with the structure of insulin-like growth factor I bound to IGF-1R reveals long-suspected differences in the way in which the critical C domain of the respective growth factors interact with IGF-1R.
Head region of the close-leg conformation of the human type 1 insulin-like growth factor receptor ectodomain in complex with human insulin-like growth factor II.
COMPLEX
#1-#2
0
MULTIPLESOURCES
2
Insulin-like growth factor 1 receptor
COMPLEX
#1
1
RECOMBINANT
3
Insulin-like growth factor II
COMPLEX
#2
1
RECOMBINANT
分子量
値: 0.25 MDa / 実験値: NO
由来(天然)
ID
Entity assembly-ID
生物種
Ncbi tax-ID
1
1
Homo sapiens (ヒト)
9606
2
2
Homo sapiens (ヒト)
9606
由来(組換発現)
ID
Entity assembly-ID
生物種
Ncbi tax-ID
細胞
プラスミド
1
1
Cricetulus griseus (モンゴルキヌゲネズミ)
10029
CHO-K1
pEE14
2
2
Escherichia coli (大腸菌)
562
緩衝液
pH: 7.5
緩衝液成分
ID
濃度
式
Buffer-ID
1
24.8mM
Tris-HCl
1
2
137mM
NaCl
1
3
2.7mM
KCl
1
4
0.02 %
NaN3
1
試料
濃度: 0.1 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES / 詳細: IGFII:IGF-1R molar ratio 1.5:1