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- EMDB-6778: Large subunit of Toxoplasma gondii ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-6778
TitleLarge subunit of Toxoplasma gondii ribosome
Map data
Sample
  • Complex: Large subunit of Toxoplasma gondii ribosome
    • RNA: x 3 types
    • Protein or peptide: x 41 types
Biological speciesToxoplasma gondii (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.17 Å
AuthorsLi Z / Guo Q / Zheng L / Ji Y / Xie Y / Lai D / Lun Z / Suo X / Gao N
CitationJournal: Cell Res / Year: 2017
Title: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.
Authors: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao /
Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.
History
DepositionJul 3, 2017-
Header (metadata) releaseAug 30, 2017-
Map releaseAug 30, 2017-
UpdateOct 18, 2017-
Current statusOct 18, 2017Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5xxb
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_6778.png

Downloads & links

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Map

FileDownload / File: emd_6778.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.32 Å
Density
Contour LevelBy AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum-0.21132639 - 0.35448036
Average (Standard dev.)0.0006059298 (±0.009347305)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 422.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z422.400422.400422.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2110.3540.001

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Supplemental data

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Mask #1

Fileemd_6778_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Large subunit of Toxoplasma gondii ribosome

EntireName: Large subunit of Toxoplasma gondii ribosome
Components
  • Complex: Large subunit of Toxoplasma gondii ribosome
    • RNA: 25S RNA
    • RNA: 5S RNA5S ribosomal RNA
    • RNA: 5.8S RNA
    • Protein or peptide: Ribosomal protein uL2
    • Protein or peptide: Ribosomal protein uL3
    • Protein or peptide: Ribosomal protein uL4
    • Protein or peptide: Ribosomal protein uL18
    • Protein or peptide: Ribosomal protein eL6
    • Protein or peptide: Ribosomal protein uL30
    • Protein or peptide: Ribosomal protein eL8
    • Protein or peptide: Ribosomal protein uL6
    • Protein or peptide: Ribosomal protein uL16
    • Protein or peptide: Ribosomal protein uL5
    • Protein or peptide: Ribosomal protein eL13
    • Protein or peptide: Ribosomal protein eL14
    • Protein or peptide: Ribosomal protein eL15
    • Protein or peptide: Ribosomal protein uL13
    • Protein or peptide: Ribosomal protein uL22
    • Protein or peptide: Ribosomal protein eL18
    • Protein or peptide: Ribosomal protein eL19
    • Protein or peptide: Ribosomal protein eL20
    • Protein or peptide: Ribosomal protein eL21
    • Protein or peptide: Ribosomal protein eL22
    • Protein or peptide: Ribosomal protein uL14
    • Protein or peptide: Ribosomal protein eL24
    • Protein or peptide: Ribosomal protein uL23
    • Protein or peptide: Ribosomal protein uL24
    • Protein or peptide: Ribosomal protein eL27
    • Protein or peptide: Ribosomal protein uL15
    • Protein or peptide: Ribosomal protein eL29
    • Protein or peptide: Ribosomal protein eL30
    • Protein or peptide: Ribosomal protein eL31
    • Protein or peptide: Ribosomal protein eL32
    • Protein or peptide: Ribosomal protein eL33
    • Protein or peptide: Ribosomal protein eL34
    • Protein or peptide: Ribosomal protein uL29
    • Protein or peptide: Ribosomal protein eL36
    • Protein or peptide: Ribosomal protein eL37
    • Protein or peptide: Ribosomal protein eL38
    • Protein or peptide: Ribosomal protein eL39
    • Protein or peptide: Ribosomal protein eL40
    • Protein or peptide: Ribosomal protein eL42
    • Protein or peptide: Ribosomal protein eL43
    • Protein or peptide: Ribosomal protein eL28

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Supramolecule #1: Large subunit of Toxoplasma gondii ribosome

SupramoleculeName: Large subunit of Toxoplasma gondii ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Toxoplasma gondii (eukaryote)

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Macromolecule #1: 25S RNA

MacromoleculeName: 25S RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 1.120153125 MDa
SequenceString: AUCAGACCUG AAAUCAGGCG AGGUUACCCG CUGAACUUAA GCAUAUUAUU AAGCGGAGGA GAAGAAAAUA ACUAUGAUUC CCUUAGUAA CGGCGAGUGA ACCGGGAUCA GCUCAAAGUG GAAAUCAACU GCUCUUUCCG AGCUGUUGAC UUGUAGCCUC G AGAGGCGU ...String:
AUCAGACCUG AAAUCAGGCG AGGUUACCCG CUGAACUUAA GCAUAUUAUU AAGCGGAGGA GAAGAAAAUA ACUAUGAUUC CCUUAGUAA CGGCGAGUGA ACCGGGAUCA GCUCAAAGUG GAAAUCAACU GCUCUUUCCG AGCUGUUGAC UUGUAGCCUC G AGAGGCGU UACCAGUGGA AGCGCAGGGU UAAGUUUCUU GGGAAAGAAC AUCAUAGAGG GUGAGAAUCC CGUCCUUCCC CU GCGUUUU UUGUUCCACG UACGGUACGC UUUUAUAGAG UCGCGUUCAU CGGGAUUUGA GCGCAAAAUG UGUGGUAGGU UUC ACAUAA AGCUAAGUAC UGGCGCGAGA CCGAUAGCAA ACAAGUACCG UGAGGGAAAG AUGAAAAGUU CUUUGGAAAG AGAG UUAAA AGUGCCUGAA AUUGCUGAAA AGGAAGCGAU UGGGACCUUU UUGAUUCCAA GAAGUGCCGC CGGUUUGCCA UCUAU CCCU UUGCCUUCUC UCUCUCACCA GGUUUAGGCU UUGGUUUCUU GAUGUAUUUC CGGUUCAUCU UUUUGGAGAG GUCAGC AUC AGCUACUCGG AAUUGAUUUC GUUCAAAGAA GGUAGGUUCC UUGUGGACCG UUAUAUCUUU GUUCUUUCCU UUUCCUU GU GGCUGAGGAG UGUUCCUGUU UCCGAGCUCC ACUUUCGAGU ACUCGGUUUC UGUGAUGCUG GCUUAAUCGG UUCCAACC G ACCCGUCUUG AAACACGGAC CAAGGAGUCU AACAUAUGUG CGAGUAUGCG GGUUUUACUC CUGUAUGCGC AAUGAAAGU GAGAGUAGGG AGAUUUUGCU UUGCCAUUCU UCGCACCUAC GACCGACCAC GAUCUCUGAG AGAGGUUUGA GUUGGAGCAU AUCUGUUAG GACCCGAAAG AUGGUGAACU AUGCCUGAGU AGAAUGAAGU CAGGGGAAAC CCUGAUGGAG GUUCGUAGCG A UACUGACG UGCAAAUCGU UCGUCAAACU UGGGUAUAGG GGCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUUCUUC CG AAGUUUC UCUCAGGAUA GCUGGAGUUU UGUUAGUUUU AUCAGGUAAA GCGAAUGAUU AGAGGCAUCG GGGGCGCGUC GCC CUCGAC CUAUUCUCAA ACUUUAAAUG GGUAAGAUCC AGUGGUUACU UCAUUGAACU GCUGGGAUUA AUAUGAACUC CAAG UGGGC CAUUUUUGGU AAGCAGAACU GGCGAUGAGG GAUGCUCCUA ACGCUUGGUU AAGGUGCCAA AAUGCUCGCU CAUCA GAUA CCAUAAAAGG UGUUGGUUCA UUUUGACAGC AGGACGGUGG UCAUGGAAGU CGAAAUCCGC UAAGGAGUGU GUAACA ACU CACCUGCCGA AUGAACCAGC CCUGAAAAUG GAUGGCGCUG AAGCGGGCUA CCGAUACCAG GCCAUUGCUA CUAAUUA UC ACUCGACCUU UUGUAGCAAU GAGUAGGAAG ACGUGGGGAU UGUGACGAAG CGUUCGGCGU AAGCCUGCGU GGAACAGU C UCUAGUGCAG AUCUUGGUGG UAGUAGCAAA UAUUCAAAUG AGAACUUUGA AGACUGAAGU GGAGAAGGGU UCCAUGUGA ACAGCAAUUG GACAUGGGUU AGCCGGUCCU AAGCGACAGC UUAACUGCGU AUACGUUAGG UUUCUUUACC UCGUUUCGCG AAAGGGAAU CGGGUUAAUA UUCCCGAGCC AGGACGUGGA UACUGUACGG UAACGUAAGU GAACUCCUCG ACGCAGGCAG G UGCUCCGG GAAGCGUUGU CUUUGCUUUU UAACAGCCUU UUCUUUUAUA CCCUGGAAUC AGUUUAGCUG GAGAUAGGGU UA GCUGGCU GGUAGAGCAC CUUGGUUUCC AAGGUGUCCG GUGCGCUCCU GACUGCCCUU GAAAAGAGGA GGGAAAGUUG UUA UUUACA CCCCUGGCCG UACCAAUAAC CGCAUCAGGU CUCCAAGGUU AGUAGCCUCU GGUCGAUAGA AUAAGGUAGA UAAG GGAAG UCGGCAAAAU AGAUCCGUAA CUUCGGGAAA AGGAUUGGCU CUGAGGGCUG GGUACAUUGC GCCCCAGUGG CCUGU AUUU GACGGAGCUU GGGACUGCUU CAUGCUUCGC GGCGUGUUGC GGACCCGAGC ACUGUCUGUA UUGCGCUCUG GGGAAA AGG UCCAUCUCCC UUUCCGGGGU CUUGGGCUGU GCGUGUACGG UGAACAGCUA ACUCAGAACU GGAGCGGACA AGGGGAA UC CGACUGUUUA AUUAAAACAA AGCAUUGCGA UGGCCAUAAC UGGUGUUGAC GCAAUGUGAU UUCUGCCCAG UGCUCUGA A UGUCAAUGUG AUGAAAUUCA AUCAAGCGCG GGUAAACGGC GGGAGUAACU AUGACUCUCU UAAGGUAGCC AAAUGCCUC GUCAUCUAAU UAGUGACGCG CAUGAAUGGA UUAACGAGAU UCCCACUGUC CCUAUCUACU AUCUAGCGAA ACCACAGCCA AGGGAACGG GCUUGGCAGA AUCAGCGGGG AAAGAAGACC CUGUUGAGCU UGACUCUAGU CCGACUUUGU GAAAUGACUU A GGAGGUGU AGCAUAAGUG GGAGCUUCGG CGCUCGUGAA AUACCACUAC UCUUAAUGUC GUUUUACUUA UUCCGUUAAG UU GAGACUC AUGCUCUGCA UGUGUUUUGG UUUUAAGGUG GCUUUUGCCG CUGAUCUACG CGGAAGACAC AGUCAGGUUG GGA GUUUGG CUGGGGCGGC ACAUCUGUGA AACAAUAGCG CAGGUGUCCU AAGGCAAGCU CAAUGAGAAC AGAAAUCUCA UGUU GACCA AAAGGGGAAA AGCUUGCUUG AUUUUGAUUU UCAGUACGAA UACAAACUGU GAAAGCAUGG CCUAUCGAUC CUUUA GGUC UGAGAAACUU UCAGCUAGAG GUGUCAGAAA AGUUACCACA GGGAUAACUG GCUUGUGGCA GCCAAGCGUU CAUAGC GAC GUUGCUUUUU GAUCCUUCGA UGUCGGCUCU UCCUAUCAUU GAGACGCAGA AGUCUCAAAG UGUCGGAUUG UUCACCC GC CAAUAGGGAA CGUGAGCUGG GUUUAGACCG UCGUGAGACA GGUUAGUUUU ACCCUACUGA UGACUUGAUA CUGCGACA G UAAUCCAACU UAGUACGAGA GGAACAGUUG GGUCGGAUAC UUGGUAAAUG CGGCUAGCUG AAAAGCUAGU GCCGCGACG CUACCAUCCG UUGGAUUAUG ACUGAACGCC UCUAAGUCAG AAUCCAUGCU GGAUAUGCAG UGUUAUUCAC CGCCUCCUUU GUUUCAUGC AAUGAUACAG GCCUCUGGCC UGCUUCUCAU CAAAGCCCUC CGCUUCAUGC UUUUGGGCGA GAAUCUCAAU U UCUAACAU UGGAGGUGAU ACAUCCAUUG CAGACGACUU UGUUGCAACC GGGUAUUGUA AGUAUGAGAG UAGGCUUGUC UA CGAUCUA CUAAGAUUUU UCCUGCGUUG UUCGAUGUGG

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Macromolecule #2: 5S RNA

MacromoleculeName: 5S RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 39.921641 KDa
SequenceString:
GCUGUCGGUC AUACUGCGUC GAAUACACCG GAUCCCUUCA GACCUCCGAA GUUAAGCGGC GCAAGGCCCG GUUAGUACUU GGGUGGGGG ACUCCCAGGG AAUACCCUCG GGUGCUGACA GCUUU

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Macromolecule #3: 5.8S RNA

MacromoleculeName: 5.8S RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 50.78798 KDa
SequenceString:
AAAUUUUCAG CAAUGGAUGU CUUGGCUCGC GCAACGAUGA AGGACGCAGC GAACUGCGAA ACGCAAUGUG AAUUGCAGAA UUCAGUGAA UCAUCAGAUU UCUGAACGCA AAUGGCACCU UGGGGAUAUU CUCCUUGGUA CGUCUGUUUC AGUGUCUUU

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Macromolecule #4: Ribosomal protein uL2

MacromoleculeName: Ribosomal protein uL2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 28.314898 KDa
SequenceString: MGRVIRAQRK GRGSVFRAHT HKRKGEAKLR PLDFAERRGY IKGLVKDILH DPGRGAPLAE VSFRDAYRYK LNKQRMVAVE GMYTGQFIY CGKNAALTIG NILPLNKMPE GTVVSNVEEK AGDRGTLART SGTYATIVGH SDDGSKTRIR LPSGARKTVS G YSRGMVGI ...String:
MGRVIRAQRK GRGSVFRAHT HKRKGEAKLR PLDFAERRGY IKGLVKDILH DPGRGAPLAE VSFRDAYRYK LNKQRMVAVE GMYTGQFIY CGKNAALTIG NILPLNKMPE GTVVSNVEEK AGDRGTLART SGTYATIVGH SDDGSKTRIR LPSGARKTVS G YSRGMVGI VAGGGRIDKP MLKAGNAYHK YKVKRNCWPK VRGVAMNPVE HPHGGGNHQH IGHPSTVSRM AAPGQKVGLI AA RRTGLLR GGRKTKLAGK SQE

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Macromolecule #5: Ribosomal protein uL3

MacromoleculeName: Ribosomal protein uL3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 44.150738 KDa
SequenceString: MSHRKFERPR HGSLGFLPRK RCKRHRGKVK AFPKDDPSKP PHLTAFMGYK AGMTHVVREL DKPGSKLHKK EIVEAVTVVD TPPMVCVGV VGYIETPRGL RALVTVWAGH LSDECKRRFY KNWYKSKRKA FTKYAKRYGD NNKMEAELTR MKNYCSVIRA I CHTQPSKT ...String:
MSHRKFERPR HGSLGFLPRK RCKRHRGKVK AFPKDDPSKP PHLTAFMGYK AGMTHVVREL DKPGSKLHKK EIVEAVTVVD TPPMVCVGV VGYIETPRGL RALVTVWAGH LSDECKRRFY KNWYKSKRKA FTKYAKRYGD NNKMEAELTR MKNYCSVIRA I CHTQPSKT PIGSKKAHVM EIQVNGGSIA EKVDFCTKMF ETAVPVKAVF TEGEMIDVIG VTKGHGVKGV VSRWGVTRLP RK THRGLRK IACIGAWHPA RVQFQVPRHG QKGYFHRTEM NKKVYRVGNG ADPRNATTES DLTEKRITPM GGFPHYGTVN NDF LLLKGC IVGCKKRPIT FRKTLVPRTT RRALEPVNLK FIDTSAKWGH GRFQTSEEKA KFYGPLKSRA AAM

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Macromolecule #6: Ribosomal protein uL4

MacromoleculeName: Ribosomal protein uL4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 45.837258 KDa
SequenceString: MATARPLVSV YKPEDGTASG TSLMPSVFLS PLRPDLVRFV HTNMAKNRRQ PYGVAPNAGY QTSAESWGTG RAVSRIPRVP GGGTHRAGQ AAFGNMCRGG GMFAPNKTWR RWHRKVNVTQ KRHAVASAVA ATGLPALVMA RGHRIDEVPE LPLVVSEKLE K VSKTREAV ...String:
MATARPLVSV YKPEDGTASG TSLMPSVFLS PLRPDLVRFV HTNMAKNRRQ PYGVAPNAGY QTSAESWGTG RAVSRIPRVP GGGTHRAGQ AAFGNMCRGG GMFAPNKTWR RWHRKVNVTQ KRHAVASAVA ATGLPALVMA RGHRIDEVPE LPLVVSEKLE K VSKTREAV KILETLGCTA ELERVRASAK KLRAGKGKMR GRRTHMRRGP LVVYAEDNGV TRAFRNIPGV ELCKVDSLNL LQ LAPGGAL GRFCLYTASA FKRLQLLFGR HTGTGTAQLK KGYHLPRALM SNADLSRIVN SEEIQRVVRP ARVAPQKKRG QKK NLLKNH AVLCRVNPAA RNLKILARLA QTEGTKQRAL VLRKKQANRE EHKKHRQSAR RFAAEIRQAF SDKMAAELEA AARR KAEEA GAIAQASAEE E

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Macromolecule #7: Ribosomal protein uL18

MacromoleculeName: Ribosomal protein uL18 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 35.408742 KDa
SequenceString: MAFVKALKNK AYFKRYQVKY RRRRQGKTDY AARRALVLQD RNKYNAHKHR LVVRLTNKRI ICQVVYSTIE GDRVLATAES TELPRYGVK IGLTNYAAAY CTGLLLARRV LKQLGMSETF EGVEATGEEY HIEENFGERR PFKVLLDVGI VRTTVGNRVF G AMKGAADG ...String:
MAFVKALKNK AYFKRYQVKY RRRRQGKTDY AARRALVLQD RNKYNAHKHR LVVRLTNKRI ICQVVYSTIE GDRVLATAES TELPRYGVK IGLTNYAAAY CTGLLLARRV LKQLGMSETF EGVEATGEEY HIEENFGERR PFKVLLDVGI VRTTVGNRVF G AMKGAADG GLHVPHGIKK FPGYSKPEGE GEGSYDPEAH RARILGLHVA DYMRQLKEED PEKYSAQFSQ YIKNKIEADD IE AMYKNAH AQIRKNPDAV KKERAANVQN VRQGNVIKTA KGQYVRHVKL TKAQRRERVQ QKIAMIAEQM AEA

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Macromolecule #8: Ribosomal protein eL6

MacromoleculeName: Ribosomal protein eL6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 21.563535 KDa
SequenceString: MAPTAAALAK KRLRTRKPKR QLYKSPAGAA KRMAKLRSSI TPGTVLILLS GGHRGKRVVF LKQLAPSGLL LVTGPFKVNG VPLRRVNQR YVIATTTKVD ISGVDVSSIK DEQFGASKKE KFAERKLRKK QDEGMFVQQS DSKAKQALPA QFKQLQDSVD K ALLASLSK ...String:
MAPTAAALAK KRLRTRKPKR QLYKSPAGAA KRMAKLRSSI TPGTVLILLS GGHRGKRVVF LKQLAPSGLL LVTGPFKVNG VPLRRVNQR YVIATTTKVD ISGVDVSSIK DEQFGASKKE KFAERKLRKK QDEGMFVQQS DSKAKQALPA QFKQLQDSVD K ALLASLSK DKLLTQYLKT RFTLRGNMRP HEMKF

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Macromolecule #9: Ribosomal protein uL30

MacromoleculeName: Ribosomal protein uL30 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 30.187777 KDa
SequenceString: MADRYSVKLL RPMEGVPVPE RTKTKIARDV KLLNKMKQAK EAHAKKVHAQ RHALKMRTYK YVSEYRKERE NLIKLKREAK AKGGFYKEP EAKVILATRI KGINKLAPKP KMILRLFRLR QLHNAVFIKV NKATIEMLKA VQPFITYGYP TLKTIRQLIY K RGYAKVGK ...String:
MADRYSVKLL RPMEGVPVPE RTKTKIARDV KLLNKMKQAK EAHAKKVHAQ RHALKMRTYK YVSEYRKERE NLIKLKREAK AKGGFYKEP EAKVILATRI KGINKLAPKP KMILRLFRLR QLHNAVFIKV NKATIEMLKA VQPFITYGYP TLKTIRQLIY K RGYAKVGK PGAHSRIRLQ ANDIVSQHLG KYGIHGVEDL VHEIYTCGPY FKQANNFLWP FKLNSPRKGF TSKRHGYNEP RK GDWGNRE EMINELVQRM I

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Macromolecule #10: Ribosomal protein eL8

MacromoleculeName: Ribosomal protein eL8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 31.095934 KDa
SequenceString: MASMKKGSAA AKGSSGGKKK LPAAPLAGKS GKKVQKSPIF AKTPRSFRVG GNIQPRRDLS RFVKWPKYIL LQRQRRVLMQ RLKVPPAIN QFTHTLDKNQ TSQLMRLLAK YKPETRAEKK QRLLQEAERQ SAGGAAGGKK PVMIKYGINH VTDLIEIKKA K LVVIAHDV ...String:
MASMKKGSAA AKGSSGGKKK LPAAPLAGKS GKKVQKSPIF AKTPRSFRVG GNIQPRRDLS RFVKWPKYIL LQRQRRVLMQ RLKVPPAIN QFTHTLDKNQ TSQLMRLLAK YKPETRAEKK QRLLQEAERQ SAGGAAGGKK PVMIKYGINH VTDLIEIKKA K LVVIAHDV TPVELVCFIP QLCRKKEVPY CIVKGKSRLG QLVHQKNCPV LAIDNVRKED QAELEAQCKI YRAMFNDNSE VR RRWGGGI NGIKSQHAQQ KKEKLINIEL KKKMGLQLA

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Macromolecule #11: Ribosomal protein uL6

MacromoleculeName: Ribosomal protein uL6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 21.459178 KDa
SequenceString:
MKSVYACETI TIPAGVTVDV KSRVVTVKGK YGEITRAFRH LPVDIQKTKS GNRLKVEMWY GTCTDLSCIR TLCSHIKNMF TGVMKKFQY KMRFVYAHFP INVNISGNGT VVEIRNFLGE KRVRIVKMLP GVKCEKATNV KDEIALTGTD VELVSRSAAL I HQSTLVRR KDIRKFLDGI YVSETSTVEQ DA

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Macromolecule #12: Ribosomal protein uL16

MacromoleculeName: Ribosomal protein uL16 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 24.846014 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIYDAGRKR ADVDEFPGVA HLVSDEYEQI SSEALEAARV CANKYMIKNC GKDNFHLRI RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPMGLVARV DIGQILMSIR TRESNVATAV TALRRAAFKF P GRQKVFVS ...String:
MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIYDAGRKR ADVDEFPGVA HLVSDEYEQI SSEALEAARV CANKYMIKNC GKDNFHLRI RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPMGLVARV DIGQILMSIR TRESNVATAV TALRRAAFKF P GRQKVFVS NKWGFTKFTK VEYKKWQAEG RIVSDGVGAK WISTKGPLAN TFPSAADITL PVA

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Macromolecule #13: Ribosomal protein uL5

MacromoleculeName: Ribosomal protein uL5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 20.240361 KDa
SequenceString:
MVKKGEENPM RKIRIEKLTL NICVGESGDR LTRAARVLEQ LTGQRPQFSK ARFTIRSFGI RRNEKIACYV TVRGKKAEDI LEKGLKVKE YELKKKNFSD SGNFGFGIQE HIDLGIKYDP STGIYGMDFY VQLTRPGNRV AHRKRARGRV GHSHRVTKED S IKWFQQTY DGIVLNH

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Macromolecule #14: Ribosomal protein eL13

MacromoleculeName: Ribosomal protein eL13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 39.757371 KDa
SequenceString: MQVDTGFTKS ISVHWSLLPL SSDSVGLHNF LSRPQNMRCD ITRGVDESSI FRCGSRLLLF RRVTAGGARL AQRKGNSTAF RGAPYGCST LHPDNFLLFL SVSLSQISAS WTQHLGRASA EHLLVPAISW RKQSLSVVCF SSVKMVSKNN VLPNVHLHKW W QRYVKTWF ...String:
MQVDTGFTKS ISVHWSLLPL SSDSVGLHNF LSRPQNMRCD ITRGVDESSI FRCGSRLLLF RRVTAGGARL AQRKGNSTAF RGAPYGCST LHPDNFLLFL SVSLSQISAS WTQHLGRASA EHLLVPAISW RKQSLSVVCF SSVKMVSKNN VLPNVHLHKW W QRYVKTWF NQPGRKQSRR LRRQEKAAKL GVVPSGLLRP IVHPPTQRYN LKVRAGRGFT LEELKSVGLS PRVALSIGVA VD HRRRNRS AESLNANVTR LKAYLAKLVL FQRGSKAKKG LAGIPADTPK SQIQNVKHVK ISTAMPIPKA SKRCKPRAIT AEE QKFMAY ATLRKALRDC KNVGKHEKKL AAEKEANQK

+
Macromolecule #15: Ribosomal protein eL14

MacromoleculeName: Ribosomal protein eL14 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.492432 KDa
SequenceString:
MGLFTRYYQP GRLCVVQYGP DAGKLCFVVD IINQTRVLVD GAGVTGVKRQ SMPVRRIALT DQYLKIPRSV RSATLKKALE KDDVIAKFN QSSWGKRRLA KEQRANMSDF DRFKLMVILK QRRKVMQQKL KSLKK

+
Macromolecule #16: Ribosomal protein eL15

MacromoleculeName: Ribosomal protein eL15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 24.387846 KDa
SequenceString: MGAYKYLEEL WKKKQSDVLR FLLRVRTWEY RQLPAVHRCT QSTRPDKARR LGYKKKQGFV IYRVRVRRGD RKKQVHKGIV YGKPKNQGV RKQKSTRNLR AVAEEKVGRK ICGGLRVLNS YWVGQDAVYK YYEVILVDPA HNAIRNDPRI NWLCKPVMKH R ECRGLTSA ...String:
MGAYKYLEEL WKKKQSDVLR FLLRVRTWEY RQLPAVHRCT QSTRPDKARR LGYKKKQGFV IYRVRVRRGD RKKQVHKGIV YGKPKNQGV RKQKSTRNLR AVAEEKVGRK ICGGLRVLNS YWVGQDAVYK YYEVILVDPA HNAIRNDPRI NWLCKPVMKH R ECRGLTSA GKKYRGLRTK GSGAARLRPS RRACWKKRQA VRLRRYR

+
Macromolecule #17: Ribosomal protein uL13

MacromoleculeName: Ribosomal protein uL13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 31.757688 KDa
SequenceString: MWALRMYRLI FCATRGRPRR ACVGSFRRTE EISSFLCLLF FCCLERRSSE KRVQLSKDLF TRRFSKMTFK KVVVIDCQGH LLGRLASVV AKELLKGQQI ICVRCEDINI SGSLHRNRLK YQRFLRLRMN SNPRRGPYHL RAPSRILWRT IRGMLRHKVE R GQKALARL ...String:
MWALRMYRLI FCATRGRPRR ACVGSFRRTE EISSFLCLLF FCCLERRSSE KRVQLSKDLF TRRFSKMTFK KVVVIDCQGH LLGRLASVV AKELLKGQQI ICVRCEDINI SGSLHRNRLK YQRFLRLRMN SNPRRGPYHL RAPSRILWRT IRGMLRHKVE R GQKALARL QVYEGVPTMV ERKKRMVVPS ALRIVRLKPK RNFCRLGDLS SQVGWSHGDL VARLEEKRKT RSSAYYQKKK ER TKMQTEA KQFAQTTLPK DQVAFLQQYG HA

+
Macromolecule #18: Ribosomal protein uL22

MacromoleculeName: Ribosomal protein uL22 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 22.319188 KDa
SequenceString: MVKYAKEPSN ENKCCKAFGQ DLRVHFKNTH ATVQAIKKDK KGNPMKLSAA KKFLEDVMEK KRCVPFRKFT GCIGRKAQAK EFKHTQGRW PVKSCKFVLD LLRNAESNAE MKNLDVDNLV IEHIQVNRAP KGRRRTYRAH GRINPYMSQP CHIEVILREQ E QAVEKPSV ...String:
MVKYAKEPSN ENKCCKAFGQ DLRVHFKNTH ATVQAIKKDK KGNPMKLSAA KKFLEDVMEK KRCVPFRKFT GCIGRKAQAK EFKHTQGRW PVKSCKFVLD LLRNAESNAE MKNLDVDNLV IEHIQVNRAP KGRRRTYRAH GRINPYMSQP CHIEVILREQ E QAVEKPSV EGVKAKTIRL TKKALARSRV RVGGGSN

+
Macromolecule #19: Ribosomal protein eL18

MacromoleculeName: Ribosomal protein eL18 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 21.223158 KDa
SequenceString:
MGIDLERNGR KKKGGRRTVK SQNPYLRLLV KLYQFLARRT NSRFNAVVLK RLMNPKRLKA PMSLSKLARH MKGKEDHIAV LVGSVTDDI RMVHAPKLTV CALRFSETAR RRLEKAGGEC LTFDQLALRR PKGSKCVLLR AATKSREADK HFGAAPGTPG S KARPYVRS TGRKFEKARG RRKSRGYKN

+
Macromolecule #20: Ribosomal protein eL19

MacromoleculeName: Ribosomal protein eL19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 22.221574 KDa
SequenceString:
MSLQLQKRLA ASILKCGRRR VWMDPNEASD ISMANSRFSI RKLVKDNLII RKAVAVHSRY RVRLYHEAKR AGRHMGKGKR KGTREARLP AKVLWMRRQR VLRRLLKKYR DSKKIDKHMY HSMYMRCKGN QFKNKRVLIE AIHNEKNLKL KEKALQEQLE A RKSKAAAQ KEKRRMKELK KEADSPASA

+
Macromolecule #21: Ribosomal protein eL20

MacromoleculeName: Ribosomal protein eL20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 21.372252 KDa
SequenceString:
MKADPTLQQK ISQYQVVGRK QPTEAEPNPS LFRMRLFARN KVLAVSKFWY LLKKMKKVKK STGEILAVNE IREKRPTFVK NFGVWLRYD SRTGTHNMYK EVRDISQNGA VSQLYAEMAG RHRALPSNIQ IIRVAEIKAS QCRRAHMQQL FDSKLKLPAI R RIFPTPKD KKSVFCARKP TLFLH

+
Macromolecule #22: Ribosomal protein eL21

MacromoleculeName: Ribosomal protein eL21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.932977 KDa
SequenceString:
MPTSFGKRAR TRQKFSKGFR RHGMPAVSRL LVIHKKGDYV DIVCDPSVQK GMPYSYYHGR TGIVFNVTQR AVGVEVNKVV GNRVIKKRI HVRLEHVRKS RCNELFLRRV KENDAAHHEA HLKGESIKTK RQIPGPKPGC FVSGTMEVLE PAPFVESY

+
Macromolecule #23: Ribosomal protein eL22

MacromoleculeName: Ribosomal protein eL22 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.103659 KDa
SequenceString:
MAKAHMRAAG SGRGAGGKKG AAAKPVKVKF TVDCQKPVDD NIIEAKGLER FLQTHIKVDG KCNNLGDRVQ VSREKAKVFV TAELPFSKR YIKYLTKKYL KKQMLRDFMR VVSSRDTVYE LRYFQMPQED ETAA

+
Macromolecule #24: Ribosomal protein uL14

MacromoleculeName: Ribosomal protein uL14 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 14.842553 KDa
SequenceString:
MKRGRGGVGG SKMRVTLGLP VGALINCCDN SGAKNLYIIA VKGWGSCLNR LPACSLGDLV LATVKKGKPD LRKKVHMAII VRQRRAWRR AEGYFIYFED NAGVVANNKG EMKGSAVTGP VGKECAELWP KISAAATAIC

+
Macromolecule #25: Ribosomal protein eL24

MacromoleculeName: Ribosomal protein eL24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 17.644857 KDa
SequenceString:
MSAVKTTIKT DMCSFSEYRI YPGRGQRFVA KDGKVHTFIH RKEASLFRQK IKPVKLHWTL VWRRMNKKGG KDAVNKRRSR KATKTVQKA IVGLSLEDIK RTRAQKPETK AKSATAVKDA KEKSKKSKAA GVRPQSGPAQ KVQIPKQQKQ ATRGRK

+
Macromolecule #26: Ribosomal protein uL23

MacromoleculeName: Ribosomal protein uL23 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 18.600809 KDa
SequenceString:
MAPKAGASAG AKASAKKAVA GKVKKAQQAA KGLKASSSFK KARVRTNVHF FRPHTLRKSR QPKVVRRCYE AATCTFPKLA KTDKYTIVR QPLTTESAMK KIEETNTLVF LVDPRSNKRQ IKQAVKELYD VDTQRVNTLI RPDGLKKAYV RLTSDYDALD V ANKIGII

+
Macromolecule #27: Ribosomal protein uL24

MacromoleculeName: Ribosomal protein uL24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 16.353228 KDa
SequenceString:
MKFSSQVSSS RRKSRKAHFS APSSVRRKIM TAPLCKELRK KYNVRSLPIR KDDEVMVVRG HHHDREGKVT KVYRKKWVIH IERVTRDKA NGETVTIGIH PSKVVITKAK LDKDRKALLE RKSRATTKGK YTEKDVAMSQ VD

+
Macromolecule #28: Ribosomal protein eL27

MacromoleculeName: Ribosomal protein eL27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 16.841355 KDa
SequenceString:
MVKLLKSGRV VVVLNGRHAG KKGVVVNTWE GSKERQFAFC LVAGIEKAPL KVHKKMPAKK IEKRMRVKPF LKYINVNHLM PTRYTVSSD MDVKTMVPED AMRTVDDRKA AKKGLKKIFY EKFTNPVNEK VGKVSKDLLF LRKKLRF

+
Macromolecule #29: Ribosomal protein uL15

MacromoleculeName: Ribosomal protein uL15 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 16.384355 KDa
SequenceString:
MTTRLRKTRK RRGHVSAGYG RVGKHRKHPG GRGKAGGMHH MRINYDKYHP GYFGKVGMRH YHLLKNQYTC PTVNVDKLWS LVSPATLEK AEQSKDKAAV IDVTKSGYFK VLGKGDLPKI PVIVKARFFS KIAEKKIKAA GGACVLTG

+
Macromolecule #30: Ribosomal protein eL29

MacromoleculeName: Ribosomal protein eL29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 6.387679 KDa
SequenceString:
MAKSKNHTNH NQNKKAHRNG IKKPQRKRKM STKGMCPKFL RNQRACKRGM MKSD

+
Macromolecule #31: Ribosomal protein eL30

MacromoleculeName: Ribosomal protein eL30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 11.682658 KDa
SequenceString:
MAKKAKKSGS EGINSRLQLV MKSGKVNLGC KTAIHALRTG KAKLIIISNN CPALRKSEIE YYAMLSKCGV HHYHGDNNEL GTACGKLFR VSCLAVTDPG DSDIIRSVE

+
Macromolecule #32: Ribosomal protein eL31

MacromoleculeName: Ribosomal protein eL31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 14.389729 KDa
SequenceString:
MKGTTRKAQN KSLQPVCRDY TIHLHKLIHG IQFKKRAPRA LREIRRFAQK TMHTKDVRID TKLNKFIWSG GIRNVPRRVR VRIARRRND DEDSKEKFYT LVQHVPVASF ENLKTEYVNE E

+
Macromolecule #33: Ribosomal protein eL32

MacromoleculeName: Ribosomal protein eL32 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.652334 KDa
SequenceString:
MAPVSTVKRS IVKKRVKSFP RFQSDRYKRV KSSWRKPKGI DCRVRRKFKG TNTMPNIGYG SNKKTRHMLP NGFFKFLVSS PKDIELLLM HNTKFAAEIA HNISSRKRRE ILERADQLNV LVLNRSARLD TAEDE

+
Macromolecule #34: Ribosomal protein eL33

MacromoleculeName: Ribosomal protein eL33 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 12.873091 KDa
SequenceString:
MKKSGTKQPV RLYVRGIVLG YKRSKVNQYP SCSLLQLEGV NDRKDTQFYL GKRVAYVYKA KTEKQGSKYR VIWGRIRRPH GNGGIVRAK FRSNLPPSSI AGRVRVFLYP SNI

+
Macromolecule #35: Ribosomal protein eL34

MacromoleculeName: Ribosomal protein eL34 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 15.427309 KDa
SequenceString:
MAPRLVYRRH NHYATGSNRV RRVRTPGAKV VFHNVGKQPS RPKCGNCHRA LPGIPAVAPH RLRLLKKRER TVHRAYGGSR CHACVRERI VRAFLVEEQK CVKQVLQVKE KQKKDEMKAE SKKSKQKKSS KTAKA

+
Macromolecule #36: Ribosomal protein uL29

MacromoleculeName: Ribosomal protein uL29 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 14.231108 KDa
SequenceString:
MVKIRAYELR GKSQKELVKQ LEDLKKELAQ LRVAKVTGSA ASKLSKVTEV RKGIARVLTV YTQKQREEAR AAFKGKKFIP NDLRAKKTR AIRRRLTASQ TRKMTVRKMK RTQNVPKRKF ALIA

+
Macromolecule #37: Ribosomal protein eL36

MacromoleculeName: Ribosomal protein eL36 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 11.437687 KDa
SequenceString:
MSGIAVGLKR GYVVTKKATP ARPSRRPRKL SPRMSSIREI IRQVAGFAPY ERRIIELLKI GTAATTKRAL KFAKKRLGTH RRGKGKREE LQNVVSAQRR KA

+
Macromolecule #38: Ribosomal protein eL37

MacromoleculeName: Ribosomal protein eL37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 11.25621 KDa
SequenceString:
MGKAGKGTGS FGKRHGKTHF LCIRCGRRAY HTQKKTCGAC GYPNAKKRTY NWSEKAKRRH TTGTGRCRHL KDMPRRAKNN FREGTKATP VKKRKTQQK

+
Macromolecule #39: Ribosomal protein eL38

MacromoleculeName: Ribosomal protein eL38 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 9.606386 KDa
SequenceString:
MAKEIRDLRK FLLTARRPDA KRVTIVRQHK KPRATGGGAS TVTKFKIRCS RYLYTFVVED REKAQKLEGS LPPSLEKVSI PGKK

+
Macromolecule #40: Ribosomal protein eL39

MacromoleculeName: Ribosomal protein eL39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 6.172519 KDa
SequenceString:
MGSIKGLSLK KHLGRKQKQN RPIPPWIRMR TGSKIRYNAK RRHWRRTKLG M

+
Macromolecule #41: Ribosomal protein eL40

MacromoleculeName: Ribosomal protein eL40 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 14.714291 KDa
SequenceString:
MQIFVKTLTG KTITLDVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLALLAQKY NCEKKVCRKC YARLPPRATN CRKKKCGHSN QLRPKKKPKN

+
Macromolecule #42: Ribosomal protein eL42

MacromoleculeName: Ribosomal protein eL42 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 12.098689 KDa
SequenceString:
MVNVPKLRNT FCKGPKCRKH QPHKVSQYKK GKDSLVVQGK RRYDRKQKGF GGQTKPIFRK KAKTTKKIVL KLECTKCKTK RLLPIKRCK SFELGAEKKS KGGPGY

+
Macromolecule #43: Ribosomal protein eL43

MacromoleculeName: Ribosomal protein eL43 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 10.426384 KDa
SequenceString:
MAKRTKKVGV VGKYGTRYGA SLRKQVKKIE LQQHAKYSCP FCGKMATKRQ AVGIWKCRGC SKVMTGGAWT LQTAAAATVR STVARLRKQ AGEAQAA

+
Macromolecule #44: Ribosomal protein eL28

MacromoleculeName: Ribosomal protein eL28 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 14.787717 KDa
SequenceString:
MVSSELLWQC VRRNHCFIRK FNGITLSAER MNLTNKNTLK YSGIAHKQPL GLNRHGANNG CIALVTVQKC SRAMRKPTRA VQVRKFKKS KKEMSKVMKA VAERRPDLLK VANKKMKKLI RTGNSSNKKE

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 2.0 e/Å2

-
Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 108162
FSC plot (resolution estimation)

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