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基本情報
登録情報 | データベース: EMDB / ID: EMD-6776 | |||||||||
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タイトル | Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2 | |||||||||
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機能・相同性 | ![]() subthalamus development / pons development / cerebellar Purkinje cell layer development / protein insertion into mitochondrial inner membrane / blastocyst hatching / protein lipoylation / pyramidal neuron development / TP53 Regulates Metabolic Genes / response to mercury ion / Mitochondrial Fatty Acid Beta-Oxidation ...subthalamus development / pons development / cerebellar Purkinje cell layer development / protein insertion into mitochondrial inner membrane / blastocyst hatching / protein lipoylation / pyramidal neuron development / TP53 Regulates Metabolic Genes / response to mercury ion / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / mitochondrial respiratory chain complex III assembly / Cytoprotection by HMOX1 / respiratory chain complex IV assembly / thalamus development / mitochondrial respirasome assembly / response to cobalamin / respiratory chain complex IV / respiratory gaseous exchange by respiratory system / cellular response to oxygen levels / iron-sulfur cluster assembly complex / Mitochondrial protein import / : / mitochondrial large ribosomal subunit binding / regulation of oxidative phosphorylation / Respiratory electron transport / gliogenesis / : / response to alkaloid / neural precursor cell proliferation / cardiac muscle tissue development / cytochrome-c oxidase / : / [2Fe-2S] cluster assembly / oxidative phosphorylation / oxygen sensor activity / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / quinol-cytochrome-c reductase / response to copper ion / ubiquinone-6 biosynthetic process / response to glucagon / respiratory chain complex I / cellular respiration / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, cytochrome c to oxygen / cytochrome-c oxidase activity / iron-sulfur cluster assembly / azurophil granule membrane / NADH dehydrogenase activity / midbrain development / mitochondrial ribosome / hypothalamus development / mitochondrial electron transport, ubiquinol to cytochrome c / sodium ion transport / mitochondrial translation / Mitochondrial protein degradation / NADH:ubiquinone reductase (H+-translocating) / positive regulation of execution phase of apoptosis / apoptotic mitochondrial changes / RHOG GTPase cycle / endopeptidase activator activity / acyl binding / ubiquinone binding / Complex I biogenesis / acyl carrier activity / response to hyperoxia / cellular response to interferon-beta / generation of precursor metabolites and energy / quinone binding / animal organ regeneration / electron transport coupled proton transport / neurogenesis / Respiratory electron transport / : / enzyme regulator activity / ATP synthesis coupled electron transport / response to cadmium ion / negative regulation of intrinsic apoptotic signaling pathway / ATP metabolic process / cellular response to retinoic acid / extrinsic apoptotic signaling pathway / mitochondrial ATP synthesis coupled electron transport / mitochondrial respiratory chain complex I assembly / response to cAMP / : / substantia nigra development / ionotropic glutamate receptor binding / respiratory electron transport chain / reactive oxygen species metabolic process / cerebellum development / regulation of mitochondrial membrane potential / : / mitochondrial electron transport, NADH to ubiquinone / response to activity / synaptic membrane / proton motive force-driven mitochondrial ATP synthesis / Mitochondrial protein degradation / central nervous system development / fatty acid binding / NADH dehydrogenase (ubiquinone) activity 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 17.4 Å | |||||||||
![]() | Gu J / Wu M / Yang M | |||||||||
![]() | ![]() タイトル: Architecture of Human Mitochondrial Respiratory Megacomplex IIIIIV. 著者: Runyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang / ![]() 要旨: The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 387.5 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 17.7 KB 17.7 KB | 表示 表示 | ![]() |
画像 | ![]() | 20.7 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 5xtiMC ![]() 6771C ![]() 6772C ![]() 6773C ![]() 6774C ![]() 6775C ![]() 5xtbC ![]() 5xtcC ![]() 5xtdC ![]() 5xteC ![]() 5xthC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.083 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
-全体 : Human respiratory chain megacomplex-I2III2IV2
全体 | 名称: Human respiratory chain megacomplex-I2III2IV2 |
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要素 |
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-超分子 #1: Human respiratory chain megacomplex-I2III2IV2
超分子 | 名称: Human respiratory chain megacomplex-I2III2IV2 / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#70 |
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由来(天然) | 生物種: ![]() |
分子量 | 実験値: 2.9 MDa |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 1.25 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
CTF補正 | ソフトウェア - 名称: CTFFIND (ver. 3.0) |
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最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 17.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 1.4) / 使用した粒子像数: 8600 |
初期 角度割当 | タイプ: RANDOM ASSIGNMENT / ソフトウェア - 名称: RELION (ver. 1.4) |
最終 角度割当 | タイプ: RANDOM ASSIGNMENT / ソフトウェア - 名称: RELION (ver. 1.4) |