+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-23936 | |||||||||
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タイトル | Cryo-EM structure of the human SSU processome, state pre-A1 | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | Ribosomal assembly intermediate / RIBOSOME (リボソーム) | |||||||||
機能・相同性 | 機能・相同性情報 mRNA N-acetyltransferase activity / negative regulation of superoxide anion generation / preribosome / oocyte growth / nucleologenesis / tRNA N-acetyltransferase activity / tRNA wobble cytosine modification / snoRNA localization / leucine zipper domain binding / 核小体 ...mRNA N-acetyltransferase activity / negative regulation of superoxide anion generation / preribosome / oocyte growth / nucleologenesis / tRNA N-acetyltransferase activity / tRNA wobble cytosine modification / snoRNA localization / leucine zipper domain binding / 核小体 / rRNA acetylation involved in maturation of SSU-rRNA / rRNA cytidine N-acetyltransferase activity / tRNA acetylation / Pwp2p-containing subcomplex of 90S preribosome / U4atac snRNP / regulation of stem cell population maintenance / CURI complex / negative regulation of amyloid precursor protein biosynthetic process / histone H2AQ104 methyltransferase activity / t-UTP complex / UTP-C complex / Mpp10 complex / U4atac snRNA binding / rRNA (pseudouridine) methyltransferase activity / rRNA modification / pre-snoRNP complex / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D sno(s)RNA binding / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 核小体 / regulation of centrosome duplication / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / tRNA export from nucleus / rRNA methyltransferase activity / histone methyltransferase binding / regulation of transcription elongation by RNA polymerase II / positive regulation of rRNA processing / embryonic cleavage / transcription elongation factor activity / rRNA base methylation / epigenetic programming in the zygotic pronuclei / spindle assembly involved in female meiosis / rRNA primary transcript binding / cilium disassembly / blastocyst formation / negative regulation of RNA splicing / Cul4-RING E3 ubiquitin ligase complex / RNA splicing, via transesterification reactions / sno(s)RNA-containing ribonucleoprotein complex / N-acetyltransferase activity / protein localization to nucleolus / U4 snRNA binding / telomerase holoenzyme complex / box C/D methylation guide snoRNP complex / SUMOylation of RNA binding proteins / U2-type precatalytic spliceosome / neural crest cell differentiation / rRNA methylation / response to stimulus / Formation of the ternary complex, and subsequently, the 43S complex / rRNA modification in the nucleus and cytosol / box C/D snoRNP assembly / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / U3 snoRNA binding / Ribosomal scanning and start codon recognition / negative regulation of ubiquitin protein ligase activity / preribosome, small subunit precursor / Translation initiation complex formation / snoRNA binding / precatalytic spliceosome / 細胞結合 / NRAGE signals death through JNK / positive regulation of transcription by RNA polymerase I / protein acetylation / ヒドロキシル化 / RNA polymerase II complex binding / mTORC1-mediated signalling / SARS-CoV-1 modulates host translation machinery / Association of TriC/CCT with target proteins during biosynthesis / Peptide chain elongation / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Selenocysteine synthesis / Formation of a pool of free 40S subunits / ubiquitin ligase inhibitor activity / TFIID-class transcription factor complex binding / Eukaryotic Translation Termination / blastocyst development / negative regulation of apoptotic signaling pathway / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / negative regulation of telomere maintenance via telomerase / chromosome, centromeric region / Viral mRNA Translation / decidualization / ubiquitin-like ligase-substrate adaptor activity / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / MTOR 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||
データ登録者 | Vanden Broeck A / Singh S / Klinge S | |||||||||
資金援助 | 米国, 2件
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引用 | ジャーナル: Science / 年: 2021 タイトル: Nucleolar maturation of the human small subunit processome. 著者: Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge / 要旨: The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_23936.map.gz | 615.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-23936-v30.xml emd-23936.xml | 124.5 KB 124.5 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_23936_fsc.xml | 19.8 KB | 表示 | FSCデータファイル |
画像 | emd_23936.png | 161 KB | ||
マスクデータ | emd_23936_msk_1.map | 669.9 MB | マスクマップ | |
Filedesc metadata | emd-23936.cif.gz | 31.4 KB | ||
その他 | emd_23936_additional_1.map.gz emd_23936_half_map_1.map.gz emd_23936_half_map_2.map.gz | 614.3 MB 535 MB 534.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-23936 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-23936 | HTTPS FTP |
-関連構造データ
関連構造データ | 7mq8MC 7mq9C 7mqaC 7mqjC C: 同じ文献を引用 (文献) M: このマップから作成された原子モデル |
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類似構造データ | |
電子顕微鏡画像生データ | EMPIAR-10781 (タイトル: Nucleolar maturation of the human small subunit processome Data size: 74.6 TB Data #1: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 1 [micrographs - multiframe] Data #2: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 2 [micrographs - multiframe] Data #3: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 3 [micrographs - multiframe] Data #4: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 4 [micrographs - multiframe] Data #5: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 5 [micrographs - multiframe] Data #6: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 6 [micrographs - multiframe] Data #7: Aligned and averaged micrographs of human SSU processomes - Dataset 1 [micrographs - single frame] Data #8: Aligned and averaged micrographs of human SSU processomes - Dataset 2 [micrographs - single frame] Data #9: Aligned and averaged micrographs of human SSU processomes - Dataset 3 [micrographs - single frame] Data #10: Aligned and averaged micrographs of human SSU processomes - Dataset 4 [micrographs - single frame] Data #11: Aligned and averaged micrographs of human SSU processomes - Dataset 5 [micrographs - single frame] Data #12: Aligned and averaged micrographs of human SSU processomes - Dataset 6 [micrographs - single frame]) |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_23936.map.gz / 形式: CCP4 / 大きさ: 669.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-マスク #1
ファイル | emd_23936_msk_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: #1
ファイル | emd_23936_additional_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_23936_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_23936_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Human SSU processome
+超分子 #1: Human SSU processome
+分子 #1: 5'ETS rRNA
+分子 #2: 18S rRNA
+分子 #3: U3 snoRNA
+分子 #31: 5'ETS rRNA
+分子 #4: 40S ribosomal protein S18
+分子 #5: 40S ribosomal protein S4, X isoform
+分子 #6: 40S ribosomal protein S5
+分子 #7: 40S ribosomal protein S6
+分子 #8: 40S ribosomal protein S7
+分子 #9: 40S ribosomal protein S8
+分子 #10: 40S ribosomal protein S9
+分子 #11: 40S ribosomal protein S12
+分子 #12: 40S ribosomal protein S16
+分子 #13: 40S ribosomal protein S11
+分子 #14: 40S ribosomal protein S24
+分子 #15: 40S ribosomal protein S28
+分子 #16: WD repeat-containing protein 75
+分子 #17: Nucleolar protein 11
+分子 #18: U3 small nucleolar RNA-associated protein 15 homolog
+分子 #19: WD repeat-containing protein 43
+分子 #20: HEAT repeat-containing protein 1
+分子 #21: U3 small nucleolar RNA-associated protein 4 homolog
+分子 #22: Periodic tryptophan protein 2 homolog
+分子 #23: U3 small nucleolar RNA-associated protein 6 homolog
+分子 #24: WD repeat-containing protein 3
+分子 #25: Transducin beta-like protein 3
+分子 #26: U3 small nucleolar RNA-associated protein 18 homolog
+分子 #27: WD repeat-containing protein 36
+分子 #28: DDB1- and CUL4-associated factor 13
+分子 #29: WD repeat-containing protein 46
+分子 #30: U3 small nucleolar ribonucleoprotein protein IMP3
+分子 #32: U3 small nucleolar ribonucleoprotein protein MPP10
+分子 #33: Something about silencing protein 10
+分子 #34: Neuroguidin
+分子 #35: Nucleolar protein 7
+分子 #36: Uncharacterized protein C1orf131
+分子 #37: 40S ribosomal protein S13
+分子 #38: 40S ribosomal protein S14
+分子 #39: Nucleolar protein 6
+分子 #40: Ribosomal RNA-processing protein 7 homolog A
+分子 #41: RNA cytidine acetyltransferase
+分子 #42: 40S ribosomal protein S3a
+分子 #43: Protein AATF
+分子 #44: 40S ribosomal protein S15a
+分子 #45: 40S ribosomal protein S27
+分子 #46: RRP12-like protein
+分子 #47: Ubiquitin-40S ribosomal protein S27a
+分子 #48: 40S ribosomal protein S17
+分子 #49: Nucleolar protein 10
+分子 #50: KRR1 small subunit processome component homolog
+分子 #51: Nucleolar protein 56
+分子 #52: Nucleolar protein 58
+分子 #53: rRNA 2'-O-methyltransferase fibrillarin
+分子 #54: NHP2-like protein 1
+分子 #55: U3 small nucleolar RNA-interacting protein 2
+分子 #56: RNA 3'-terminal phosphate cyclase-like protein
+分子 #57: Ribosome biogenesis protein BMS1 homolog
+分子 #58: Ribosomal RNA small subunit methyltransferase NEP1
+分子 #59: rRNA-processing protein FCF1 homolog
+分子 #60: U3 small nucleolar ribonucleoprotein protein IMP4
+分子 #61: Small subunit processome component 20 homolog
+分子 #62: Deoxynucleotidyltransferase terminal-interacting protein 2
+分子 #63: 40S ribosomal protein S23
+分子 #64: U3 small nucleolar RNA-associated protein 14 homolog A
+分子 #65: Nucleolar protein 14
+分子 #66: Nucleolar complex protein 4 homolog
+分子 #67: RNA-binding protein PNO1
+分子 #68: Unassigned peptides
+分子 #69: Probable U3 small nucleolar RNA-associated protein 11
+分子 #70: Bystin
+分子 #71: MAGNESIUM ION
+分子 #72: ADENOSINE-5'-TRIPHOSPHATE
+分子 #73: ZINC ION
+分子 #74: GUANOSINE-5'-TRIPHOSPHATE
+分子 #75: S-ADENOSYL-L-HOMOCYSTEINE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.6 |
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グリッド | モデル: Quantifoil R2/2 / 材質: GOLD / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 支持フィルム - Film thickness: 3 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec. |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 90 % / チャンバー内温度: 283 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy / Cs: 0.01 mm / 最大 デフォーカス(公称値): 2.7 µm 最小 デフォーカス(公称値): 0.7000000000000001 µm |
特殊光学系 | エネルギーフィルター - スリット幅: 20 eV |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 実像数: 84904 / 平均電子線量: 58.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
-原子モデル構築 1
初期モデル |
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精密化 | 空間: REAL | ||||||||||||||||||||
得られたモデル | PDB-7mq8: |