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データを開く
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基本情報
登録情報 | データベース: EMDB / ID: EMD-23938 | |||||||||
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タイトル | Cryo-EM structure of the human SSU processome, state post-A1 | |||||||||
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![]() | Ribosomal assembly intermediate / RIBOSOME | |||||||||
機能・相同性 | ![]() U6 snRNA 2'-O-ribose methyltransferase activity / oocyte growth / nucleolar exosome (RNase complex) / nucleologenesis / 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / positive regulation of mRNA cis splicing, via spliceosome / snoRNA localization / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process ...U6 snRNA 2'-O-ribose methyltransferase activity / oocyte growth / nucleolar exosome (RNase complex) / nucleologenesis / 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / positive regulation of mRNA cis splicing, via spliceosome / snoRNA localization / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / granular component / nuclear polyadenylation-dependent antisense transcript catabolic process / RNA exonuclease activity / chromatin extrusion motor activity / ATP-dependent H2AZ histone chaperone activity / regulation of telomerase RNA localization to Cajal body / nuclear polyadenylation-dependent CUT catabolic process / positive regulation of male gonad development / ATP-dependent H3-H4 histone complex chaperone activity / U4atac snRNP / cohesin loader activity / TRAMP-dependent tRNA surveillance pathway / CURI complex / UTP-C complex / regulation of stem cell population maintenance / CUT catabolic process / exosome (RNase complex) / cytoplasmic exosome (RNase complex) / t-UTP complex / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / U4atac snRNA binding / Mpp10 complex / Pwp2p-containing subcomplex of 90S preribosome / nuclear exosome (RNase complex) / rRNA (pseudouridine) methyltransferase activity / pre-snoRNP complex / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA (adenine-N6,N6-)-dimethyltransferase activity / box C/D sno(s)RNA binding / preribosome / histone mRNA catabolic process / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / dense fibrillar component / rRNA methyltransferase activity / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear mRNA surveillance / histone methyltransferase binding / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of transcription elongation by RNA polymerase II / box C/D methylation guide snoRNP complex / positive regulation of rRNA processing / tRNA export from nucleus / DNA clamp loader activity / cilium disassembly / rRNA primary transcript binding / transcription elongation factor activity / epigenetic programming in the zygotic pronuclei / spindle assembly involved in female meiosis / rRNA base methylation / RNA splicing, via transesterification reactions / sno(s)RNA-containing ribonucleoprotein complex / blastocyst formation / positive regulation of respiratory burst involved in inflammatory response / protein localization to nucleolus / U4 snRNA binding / Cul4-RING E3 ubiquitin ligase complex / SUMOylation of RNA binding proteins / nucleolus organization / telomerase RNA binding / translation at postsynapse / U2-type precatalytic spliceosome / rRNA methylation / RNA catabolic process / histone H2AQ104 methyltransferase activity / negative regulation of RNA splicing / mammalian oogenesis stage / neural precursor cell proliferation / activation-induced cell death of T cells / neural crest cell differentiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / U3 snoRNA binding / translation at presynapse / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / rRNA modification in the nucleus and cytosol / cytoplasmic side of rough endoplasmic reticulum membrane / snoRNA binding / precatalytic spliceosome / preribosome, small subunit precursor / maturation of 5.8S rRNA / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / Translation initiation complex formation / rRNA metabolic process / positive regulation of transcription by RNA polymerase I / negative regulation of telomere maintenance via telomerase / fibroblast growth factor binding / Association of TriC/CCT with target proteins during biosynthesis 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.7 Å | |||||||||
![]() | Vanden Broeck A / Singh S / Klinge S | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Nucleolar maturation of the human small subunit processome. 著者: Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge / ![]() 要旨: The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within. | |||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 619.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 126.5 KB 126.5 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 19.7 KB | 表示 | ![]() |
画像 | ![]() | 163.1 KB | ||
マスクデータ | ![]() | 669.9 MB | ![]() | |
Filedesc metadata | ![]() | 31.3 KB | ||
その他 | ![]() ![]() ![]() | 616.4 MB 536.2 MB 536.3 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7mqaMC ![]() 7mq8C ![]() 7mq9C ![]() 7mqjC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data size: 74.6 TB Data #1: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 1 [micrographs - multiframe] Data #2: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 2 [micrographs - multiframe] Data #3: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 3 [micrographs - multiframe] Data #4: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 4 [micrographs - multiframe] Data #5: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 5 [micrographs - multiframe] Data #6: Unaligned multi-frame micrograph movies of human SSU processomes - Dataset 6 [micrographs - multiframe] Data #7: Aligned and averaged micrographs of human SSU processomes - Dataset 1 [micrographs - single frame] Data #8: Aligned and averaged micrographs of human SSU processomes - Dataset 2 [micrographs - single frame] Data #9: Aligned and averaged micrographs of human SSU processomes - Dataset 3 [micrographs - single frame] Data #10: Aligned and averaged micrographs of human SSU processomes - Dataset 4 [micrographs - single frame] Data #11: Aligned and averaged micrographs of human SSU processomes - Dataset 5 [micrographs - single frame] Data #12: Aligned and averaged micrographs of human SSU processomes - Dataset 6 [micrographs - single frame]) |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-マスク #1
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密度ヒストグラム |
-追加マップ: #1
ファイル | emd_23938_additional_1.map | ||||||||||||
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_23938_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_23938_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Human SSU processome
+超分子 #1: Human SSU processome
+分子 #1: 5'ETS rRNA
+分子 #2: 18S rRNA
+分子 #3: U3 snoRNA
+分子 #4: 40S ribosomal protein S18
+分子 #5: 40S ribosomal protein S4, X isoform
+分子 #6: 40S ribosomal protein S5
+分子 #7: 40S ribosomal protein S6
+分子 #8: 40S ribosomal protein S7
+分子 #9: 40S ribosomal protein S8
+分子 #10: 40S ribosomal protein S9
+分子 #11: 40S ribosomal protein S12
+分子 #12: 40S ribosomal protein S16
+分子 #13: 40S ribosomal protein S11
+分子 #14: 40S ribosomal protein S24
+分子 #15: 40S ribosomal protein S28
+分子 #16: WD repeat-containing protein 75
+分子 #17: Nucleolar protein 11
+分子 #18: U3 small nucleolar RNA-associated protein 15 homolog
+分子 #19: WD repeat-containing protein 43
+分子 #20: HEAT repeat-containing protein 1
+分子 #21: U3 small nucleolar RNA-associated protein 4 homolog
+分子 #22: Periodic tryptophan protein 2 homolog
+分子 #23: U3 small nucleolar RNA-associated protein 6 homolog
+分子 #24: WD repeat-containing protein 3
+分子 #25: Transducin beta-like protein 3
+分子 #26: U3 small nucleolar RNA-associated protein 18 homolog
+分子 #27: WD repeat-containing protein 36
+分子 #28: DDB1- and CUL4-associated factor 13
+分子 #29: WD repeat-containing protein 46
+分子 #30: Active regulator of SIRT1
+分子 #31: U3 small nucleolar ribonucleoprotein protein IMP3
+分子 #32: U3 small nucleolar ribonucleoprotein protein MPP10
+分子 #33: Something about silencing protein 10
+分子 #34: Nucleolar protein 7
+分子 #35: 40S ribosomal protein S13
+分子 #36: 40S ribosomal protein S14
+分子 #37: Nucleolar protein 6
+分子 #38: Ribosomal RNA-processing protein 7 homolog A
+分子 #39: Probable dimethyladenosine transferase
+分子 #40: 40S ribosomal protein S3a
+分子 #41: 40S ribosomal protein S15a
+分子 #42: 40S ribosomal protein S19
+分子 #43: 40S ribosomal protein S27
+分子 #44: RRP12-like protein
+分子 #45: Probable ATP-dependent RNA helicase DHX37
+分子 #46: Ubiquitin-40S ribosomal protein S27a
+分子 #47: 40S ribosomal protein S17
+分子 #48: Exosome component 10
+分子 #49: Nucleolar protein 56
+分子 #50: Nucleolar protein 58
+分子 #51: rRNA 2'-O-methyltransferase fibrillarin
+分子 #52: NHP2-like protein 1
+分子 #53: U3 small nucleolar RNA-interacting protein 2
+分子 #54: RNA 3'-terminal phosphate cyclase-like protein
+分子 #55: Ribosome biogenesis protein BMS1 homolog
+分子 #56: Ribosomal RNA small subunit methyltransferase NEP1
+分子 #57: rRNA-processing protein FCF1 homolog
+分子 #58: U3 small nucleolar ribonucleoprotein protein IMP4
+分子 #59: Small subunit processome component 20 homolog
+分子 #60: Deoxynucleotidyltransferase terminal-interacting protein 2
+分子 #61: 40S ribosomal protein S23
+分子 #62: U3 small nucleolar RNA-associated protein 14 homolog A
+分子 #63: Nucleolar protein 14
+分子 #64: Nucleolar complex protein 4 homolog
+分子 #65: RNA-binding protein PNO1
+分子 #66: Unassigned peptides
+分子 #67: Probable U3 small nucleolar RNA-associated protein 11
+分子 #68: Bystin
+分子 #69: MAGNESIUM ION
+分子 #70: ADENOSINE-5'-TRIPHOSPHATE
+分子 #71: ZINC ION
+分子 #72: GUANOSINE-5'-TRIPHOSPHATE
+分子 #73: S-ADENOSYL-L-HOMOCYSTEINE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.6 |
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グリッド | モデル: Quantifoil R2/2 / 材質: GOLD / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 支持フィルム - Film thickness: 3 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec. |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 90 % / チャンバー内温度: 283 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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特殊光学系 | エネルギーフィルター - スリット幅: 20 eV |
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 実像数: 84904 / 平均電子線量: 58.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 0.01 mm / 最大 デフォーカス(公称値): 2.7 µm 最小 デフォーカス(公称値): 0.7000000000000001 µm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
初期モデル |
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精密化 | 空間: REAL | ||||||||||||||||||||||||
得られたモデル | ![]() PDB-7mqa: |