Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge /
PubMed Abstract
The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within.
EMDB-23938, PDB-7mqa: Cryo-EM structure of the human SSU processome, state post-A1 Method: EM (single particle) / Resolution: 2.7 Å
EMDB-23939: Cryo-EM structure of the human SSU processome, state pre-A1 - raw maps Method: EM (single particle) / Resolution: 3.51 Å
EMDB-23940: Cryo-EM structure of the human SSU processome, state post-A1 - raw maps Method: EM (single particle) / Resolution: 2.65 Å
EMDB-24149: Cryo-EM structure of the human SSU processome, state pre-A1 - 5'ETS focused map Method: EM (single particle) / Resolution: 3.73 Å
EMDB-24150: Cryo-EM structure of the human SSU processome, state pre-A1 - Core focused map Method: EM (single particle) / Resolution: 3.29 Å
EMDB-24151: Cryo-EM structure of the human SSU processome, state pre-A1 - HEATR1 focused map Method: EM (single particle) / Resolution: 6.37 Å
EMDB-24152: Cryo-EM structure of the human SSU processome, state pre-A1 - NAT10-NOL10 focused map Method: EM (single particle) / Resolution: 3.67 Å
EMDB-24153: Cryo-EM structure of the human SSU processome, state pre-A1 - NEP1 focused map Method: EM (single particle) / Resolution: 3.54 Å
EMDB-24154: Cryo-EM structure of the human SSU processome, state pre-A1 - NGDN focused map Method: EM (single particle) / Resolution: 3.83 Å
EMDB-24155: Cryo-EM structure of the human SSU processome, state pre-A1 - PNO1 focused map Method: EM (single particle) / Resolution: 4.06 Å
EMDB-24156: Cryo-EM structure of the human SSU processome, state pre-A1 - TBL3 focused map Method: EM (single particle) / Resolution: 4.09 Å
EMDB-24157: Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-CTD focused map Method: EM (single particle) / Resolution: 6.3 Å
EMDB-24158: Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-NTD focused map Method: EM (single particle) / Resolution: 3.42 Å
EMDB-24159: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPA focused map Method: EM (single particle) / Resolution: 3.58 Å
EMDB-24160: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC focused map Method: EM (single particle) / Resolution: 4.45 Å
EMDB-24161: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC-Central focused map Method: EM (single particle) / Resolution: 3.73 Å
EMDB-24162: Cryo-EM structure of the human SSU processome, state post-A1 - Core focused map Method: EM (single particle) / Resolution: 2.63 Å
EMDB-24163: Cryo-EM structure of the human SSU processome, state post-A1 - DHX37 focused map Method: EM (single particle) / Resolution: 4.23 Å
EMDB-24164: Cryo-EM structure of the human SSU processome, state post-A1 - H44 focused map Method: EM (single particle) / Resolution: 3.87 Å
EMDB-24165: Cryo-EM structure of the human SSU processome, state post-A1 - HEATR1 focused map Method: EM (single particle) / Resolution: 3.2 Å
EMDB-24166: Cryo-EM structure of the human SSU processome, state post-A1 - probable MYBBP1A focused map Method: EM (single particle) / Resolution: 3.71 Å
EMDB-24167: Cryo-EM structure of the human SSU processome, state post-A1 - NOL11 focused map Method: EM (single particle) / Resolution: 3.57 Å
EMDB-24168: Cryo-EM structure of the human SSU processome, state post-A1 - RPS19-AROS focused map Method: EM (single particle) / Resolution: 2.99 Å
EMDB-24169: Cryo-EM structure of the human SSU processome, state post-A1 - UTP20 focused map Method: EM (single particle) / Resolution: 2.72 Å
EMDB-24170: Cryo-EM structure of the human SSU processome, state post-A1 - UTP6 focused map Method: EM (single particle) / Resolution: 2.96 Å
EMDB-24171: Cryo-EM structure of the human SSU processome, state post-A1 - UTPA focused map Method: EM (single particle) / Resolution: 2.78 Å
EMDB-24172: Cryo-EM structure of the human SSU processome, state post-A1 - UTPC focused map Method: EM (single particle) / Resolution: 2.89 Å
EMDB-24173: Cryo-EM structure of the human SSU processome, state post-A1 - UTPC-central focused map Method: EM (single particle) / Resolution: 2.6 Å
EMDB-24174: Cryo-EM structure of the human SSU processome - BYST-RPS12 focused map Method: EM (single particle) / Resolution: 3.46 Å
EMDB-24175: Cryo-EM structure of the human SSU processome - NOC4L-NOP14 focused map Method: EM (single particle) / Resolution: 3.41 Å
EMDB-24176: Cryo-EM structure of the human SSU processome - RRP12-BYST focused map Method: EM (single particle) / Resolution: 5.25 Å
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)
EMDB accession codes are about to change! (news from PDBe EMDB page)
The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
The EM Navigator/Yorodumi systems omit the EMD- prefix.
Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator