|Entry||Database: EMDB / ID: EMD-24158|
|Title||Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-NTD focused map|
|Sample||Human SSU processome, state post-A1|
|Biological species||Homo sapiens (human)|
|Method||single particle reconstruction / cryo EM / Resolution: 3.42 Å|
|Authors||Vanden Broeck A / Singh S / Klinge S|
|Funding support|| United States, European Union, 2 items |
|Citation||Journal: Science / Year: 2021|
Title: Nucleolar maturation of the human small subunit processome.
Authors: Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge /
Abstract: [Figure: see text].
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_24158.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.08 Å|
|Symmetry||Space group: 1|
CCP4 map header:
|Projections & Slices|
-Additional map: #1
|Projections & Slices|
-Half map: #1
|Projections & Slices|
-Half map: #2
-Entire Human SSU processome, state post-A1
|Entire||Name: Human SSU processome, state post-A1 / Number of Components: 1|
-Component #1: protein, Human SSU processome, state post-A1
|Protein||Name: Human SSU processome, state post-A1 / Recombinant expression: No|
|Mass||Theoretical: 5 MDa|
|Source||Species: Homo sapiens (human)|
|Specimen||Specimen State: Particle / Method: cryo EM|
|Sample solution||pH: 7.6|
|Vitrification||Instrument: FEI VITROBOT MARK IV / Cryogen Name: ETHANE / Temperature: 283 K / Humidity: 90 %|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron Source: FIELD EMISSION GUN / Accelerating Voltage: 300 kV / Electron Dose: 58 e/Å2 / Illumination Mode: FLOOD BEAM|
|Lens||Cs: 0.01 mm / Imaging Mode: BRIGHT FIELD / Defocus: 700.0 - 2700.0 nm|
|Specimen Holder||Model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Image acquisition||Number of Digital Images: 84904|
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