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Structure paper

TitleIn-cell structural insight into the stability of sperm microtubule doublet.
Journal, issue, pagesCell Discov, Vol. 9, Issue 1, Page 116, Year 2023
Publish dateNov 21, 2023
AuthorsLinhua Tai / Guoliang Yin / Xiaojun Huang / Fei Sun / Yun Zhu /
PubMed AbstractThe propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. ...The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of intact sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT at 4.5-7.5 Å resolutions, and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of intact sperm DMT in 48 nm periodicity that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies.
External linksCell Discov / PubMed:37989994 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution4.5 - 14.08 Å
Structure data

EMDB-35210: In situ structure of A-tubule of axonemal doublet microtubules in mouse sperm with 16-nm repeat
Method: EM (subtomogram averaging) / Resolution: 4.5 Å

EMDB-35211: In situ structure of B-tubule of axonemal doublet microtubules in mouse sperm with 16-nm repeat
Method: EM (subtomogram averaging) / Resolution: 6.5 Å

EMDB-35222: A1 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 6.5 Å

EMDB-35224: A2 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 6.6 Å

EMDB-35225: A3 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 6.6 Å

EMDB-35226: B1 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.5 Å

EMDB-35227: B2 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.5 Å

EMDB-35228: B3 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.5 Å

35229

8i7o

EMDB-35229, PDB-8i7o:
In situ structure of axonemal doublet microtubules in mouse sperm with 16-nm repeat
Method: EM (subtomogram averaging) / Resolution: 4.5 Å

35230

8i7r

EMDB-35230, PDB-8i7r:
In situ structure of axonemal doublet microtubules in mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 6.5 Å

EMDB-35231: Half of axonemal doublet microtubules in mouse sperm with 96-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.7 Å

EMDB-35232: Half of axonemal doublet microtubules in mouse sperm with 96-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.7 Å

EMDB-35236: Axonemal doublet microtubules in mouse sperm with 96-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.7 Å

EMDB-35237: Axonemal doublet microtubules in deformed mouse sperm with 16-nm repeat
Method: EM (subtomogram averaging) / Resolution: 7.9 Å

EMDB-35238: Axonemal doublet microtubules in deformed mouse sperm with 48-nm repeat
Method: EM (subtomogram averaging) / Resolution: 8.6 Å

EMDB-37643: Consensus map of axonemal doublet microtubules in mouse sperm with 96-nm repeat
Method: EM (subtomogram averaging) / Resolution: 14.08 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Source
  • mus musculus (house mouse)
KeywordsSTRUCTURAL PROTEIN / microtubules / axoneme / sperm / filament

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