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- EMDB-35229: In situ structure of axonemal doublet microtubules in mouse sperm... -

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Basic information

Entry
Database: EMDB / ID: EMD-35229
TitleIn situ structure of axonemal doublet microtubules in mouse sperm with 16-nm repeat
Map dataAxonemal doublet microtubules in mouse sperm with 16-nm repeat
Sample
  • Cell: mouse sperm
    • Protein or peptide: x 24 types
  • Ligand: x 1 types
Keywordsmicrotubules / axoneme / sperm / filament / STRUCTURAL PROTEIN
Function / homology
Function and homology information


male germ-line stem cell population maintenance / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / 9+0 motile cilium / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / axonemal microtubule doublet inner sheath / axonemal B tubule inner sheath ...male germ-line stem cell population maintenance / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / 9+0 motile cilium / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / axonemal microtubule doublet inner sheath / axonemal B tubule inner sheath / axonemal A tubule inner sheath / Intraflagellar transport / inner dynein arm assembly / Carboxyterminal post-translational modifications of tubulin / protein polyglutamylation / positive regulation of feeding behavior / sperm principal piece / COPI-independent Golgi-to-ER retrograde traffic / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / microtubule sliding / MAP kinase tyrosine/serine/threonine phosphatase activity / cilium movement involved in cell motility / regulation of cilium beat frequency involved in ciliary motility / COPI-mediated anterograde transport / Aggrephagy / protein localization to organelle / 9+2 motile cilium / Kinesins / acrosomal membrane / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / PKR-mediated signaling / Resolution of Sister Chromatid Cohesion / The role of GTSE1 in G2/M progression after G2 checkpoint / RHO GTPases activate IQGAPs / axoneme assembly / Recycling pathway of L1 / cilium organization / axonemal microtubule / COPI-dependent Golgi-to-ER retrograde traffic / RHO GTPases Activate Formins / Separation of Sister Chromatids / Hedgehog 'off' state / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Regulation of PLK1 Activity at G2/M Transition / manchette / flagellated sperm motility / MHC class II antigen presentation / cell projection organization / protein targeting to mitochondrion / protein targeting to membrane / microtubule organizing center / extrinsic component of membrane / ciliary base / tubulin complex / positive regulation of cell motility / myosin phosphatase activity / intercellular bridge / protein-serine/threonine phosphatase / cytoplasmic microtubule / beta-tubulin binding / phosphatase activity / regulation of cell division / axoneme / mitotic cytokinesis / phosphoprotein phosphatase activity / peptidyl-tyrosine dephosphorylation / spermatid development / cilium assembly / alpha-tubulin binding / single fertilization / sperm flagellum / cellular response to unfolded protein / microtubule-based process / sperm midpiece / Hsp70 protein binding / Neutrophil degranulation / centriole / protein-tyrosine-phosphatase / mitotic spindle organization / acrosomal vesicle / protein tyrosine phosphatase activity / ciliary basal body / cellular response to leukemia inhibitory factor / Hsp90 protein binding / G protein-coupled receptor binding / cilium / mitochondrial intermembrane space / structural constituent of cytoskeleton / mitotic spindle / SH3 domain binding / spindle pole / intracellular calcium ion homeostasis / calcium-dependent protein binding / microtubule cytoskeleton
Similarity search - Function
: / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Sperm-associated microtubule inner protein 10 / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Tektin bundle interacting protein 1 / DJBP, EF-hand domain / : / EF-hand domain ...: / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Sperm-associated microtubule inner protein 10 / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Tektin bundle interacting protein 1 / DJBP, EF-hand domain / : / EF-hand domain / Sperm acrosome-associated protein 9 / Sperm acrosome-associated protein 9 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Flattop / : / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / : / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / EF hand / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain profile. / Dual specificity protein phosphatase domain / EF-hand domain pair / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Protein-tyrosine phosphatase-like / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Cilia- and flagella-associated protein 77 / Tektin bundle-interacting protein 1 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Tektin-4 / Cilia- and flagella-associated protein 52 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Enkurin ...Cilia- and flagella-associated protein 77 / Tektin bundle-interacting protein 1 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Tektin-4 / Cilia- and flagella-associated protein 52 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Enkurin / Tektin-3 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 20 / Tektin-2 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / Dual specificity phosphatase 21 / Sperm-associated microtubule inner protein 10 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 276 / Tektin-1 / Parkin coregulated gene protein homolog / Ciliary microtubule inner protein 2C
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsubtomogram averaging / cryo EM / Resolution: 4.5 Å
AuthorsZhu Y / Yin GL / Tai LH / Sun F
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31925026 China
CitationJournal: Cell Discov / Year: 2023
Title: In-cell structural insight into the stability of sperm microtubule doublet.
Authors: Linhua Tai / Guoliang Yin / Xiaojun Huang / Fei Sun / Yun Zhu /
Abstract: The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. ...The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of intact sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT at 4.5-7.5 Å resolutions, and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of intact sperm DMT in 48 nm periodicity that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies.
History
DepositionFeb 1, 2023-
Header (metadata) releaseOct 11, 2023-
Map releaseOct 11, 2023-
UpdateDec 6, 2023-
Current statusDec 6, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_35229.png

Downloads & links

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Map

FileDownload / File: emd_35229.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationAxonemal doublet microtubules in mouse sperm with 16-nm repeat
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.76 Å/pix.
x 320 pix.
= 563.2 Å		1.76 Å/pix.
x 320 pix.
= 563.2 Å		1.76 Å/pix.
x 320 pix.
= 563.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.76 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.004
Minimum - Maximum-0.003409233 - 0.013886109
Average (Standard dev.)0.00070860336 (±0.0015342648)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 563.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : mouse sperm

EntireName: mouse sperm
Components
  • Cell: mouse sperm
    • Protein or peptide: Tektin-1
    • Protein or peptide: Detyrosinated tubulin alpha-3 chain
    • Protein or peptide: Tubulin beta-4B chain
    • Protein or peptide: Tektin-2
    • Protein or peptide: Tektin-3
    • Protein or peptide: Tektin-4
    • Protein or peptide: Tektin bundle-interacting protein 1
    • Protein or peptide: Tektin-5
    • Protein or peptide: EF-hand domain-containing protein 1
    • Protein or peptide: EF-hand domain-containing family member C2
    • Protein or peptide: Protein FAM166A
    • Protein or peptide: Protein FAM166C
    • Protein or peptide: Dual specificity phosphatase 21
    • Protein or peptide: Coiled-coil domain-containing protein 105
    • Protein or peptide: Enkurin
    • Protein or peptide: Testis-expressed protein 43
    • Protein or peptide: Cilia- and flagella-associated protein 276
    • Protein or peptide: Protein Flattop
    • Protein or peptide: Cilia- and flagella-associated protein 52
    • Protein or peptide: EF-hand calcium-binding domain-containing protein 6
    • Protein or peptide: Cilia and flagella-associated protein 77
    • Protein or peptide: Cilia- and flagella-associated protein 20
    • Protein or peptide: Parkin coregulated gene protein homolog
    • Protein or peptide: Sperm acrosome-associated protein 9
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: mouse sperm

SupramoleculeName: mouse sperm / type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#24
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Tektin-1

MacromoleculeName: Tektin-1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 48.713035 KDa
SequenceString: MAKLLQSPPR FLPEEWYIAN KSQYHRAEAQ RSQSERLVAE SQRLVEEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVNQTD DLLTYKTRLE RSLESYKEPL HITEKCLEYR EKRVGIDLVH DVVEQELQKE ADIIHGVMNL LIRTLEESTE Q IRLNRSAK ...String:
MAKLLQSPPR FLPEEWYIAN KSQYHRAEAQ RSQSERLVAE SQRLVEEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVNQTD DLLTYKTRLE RSLESYKEPL HITEKCLEYR EKRVGIDLVH DVVEQELQKE ADIIHGVMNL LIRTLEESTE Q IRLNRSAK YNLEKDLRDK FTAITIDDVC FSLNNNSPNI NFSEKVVRIE PNSVSLEDWL DFSNANVEKA DKQLNNSTAL KT LVDQILS QTANDLRRQC EVVDEAFING LKETKDARNK LADHLAKVME EIASQEKNIM ALENAITQQE GPAKVAHTRL ETR THRPNV ELCRDIAQYR LIKEIQEINH NVARLKETLA QAQTQLKALY RRQLALQEEI QVKENTIYID QVLCMEMRKS IPPR DGDDH GAWEGGIRAE AIC

UniProtKB: Tektin-1

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Macromolecule #2: Detyrosinated tubulin alpha-3 chain

MacromoleculeName: Detyrosinated tubulin alpha-3 chain / type: protein_or_peptide / ID: 2 / Number of copies: 61 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 48.68909 KDa
SequenceString: MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIVDLVL DRIRKLADLC TGLQGFLIFH SFGGGTGSGF ASLLMERLSV D YGKKSKLE ...String:
MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKEIVDLVL DRIRKLADLC TGLQGFLIFH SFGGGTGSGF ASLLMERLSV D YGKKSKLE FAIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCM LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GVD

UniProtKB: Tubulin alpha-3 chain

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Macromolecule #3: Tubulin beta-4B chain

MacromoleculeName: Tubulin beta-4B chain / type: protein_or_peptide / ID: 3 / Number of copies: 62 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 47.897918 KDa
SequenceString: MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS ...String:
MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QD

UniProtKB: Tubulin beta-4B chain

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Macromolecule #4: Tektin-2

MacromoleculeName: Tektin-2 / type: protein_or_peptide / ID: 4 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 50.385066 KDa
SequenceString: MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN RTRLAERIDT VNRWKETLDK CLTDLDAEI DSLAQAKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV RDPVEEELLK EVEVIEATKK VLQEKISQAF Q HLCLLQEI ...String:
MATLSFKPSE RYRLSDWRTN SYLLSTNAER QRDASHQIRQ EARILRNETN NQIVWDEHDN RTRLAERIDT VNRWKETLDK CLTDLDAEI DSLAQAKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV RDPVEEELLK EVEVIEATKK VLQEKISQAF Q HLCLLQEI RQQLNSDHRD KMETLEIDRG CLSLNLTSPN ISLKVNPTRI PKDSTTLQQW DEFTRFNKNR AEAEMKASIE LR EAIALAI AQTNNELDAQ RVATEFTFRK RLREMESFYS ELKWQEKNTL EEIAELQGDI RRLEEDLRRK MMNLKLAHTR LES RTYRSN VELCRDQTQY GLIDEVHQLE ATINTMKQKL AQTQNALDAL FKHLARIQAD IACKTNTLLL DTKCMDTRRK LTVP AEKFV PQVDTFTRTT NRTLSPLKIC QLELT

UniProtKB: Tektin-2

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Macromolecule #5: Tektin-3

MacromoleculeName: Tektin-3 / type: protein_or_peptide / ID: 5 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 56.754777 KDa
SequenceString: MELLGSTLTA TYAHPPPASA SFLPAIGTIT SSYKDRFPHR NLTHSLSLPW RPNTYYKTAY NYPTLAPYSS RSQRVCESTM LPFVSNRTT FFTRYTPDDW YRSNLVSFQE SNSSRHNSER LRVDTSRLIQ DKYQQIRKTQ AHSTQNLGER VNDLAFWKSE I THELDEMI ...String:
MELLGSTLTA TYAHPPPASA SFLPAIGTIT SSYKDRFPHR NLTHSLSLPW RPNTYYKTAY NYPTLAPYSS RSQRVCESTM LPFVSNRTT FFTRYTPDDW YRSNLVSFQE SNSSRHNSER LRVDTSRLIQ DKYQQIRKTQ AHSTQNLGER VNDLAFWKSE I THELDEMI GETNALTDIK RRLERGLIET EGPLQVSREC LFHREKRMGI DLVHDEAEKE LLAEVDTILC CQERMRQHLD KA NAQLASD RSAQHELEKD LSDKQAALRI DDKCQHLRNT SEGVSYFRGV ERVDATVSVP ETWAKFTDDN VLRSQSERAA SAK LREETE NLLIVTANEM WNQFNKVNLA FTNRIAETVD AKNKIHTHLT KTLQEIFQIE MTIESIKKAI KEKSAFLKVA QTRL DERTR RPNVELCRDM AQLRLVNEVY EVDETIQTLQ QRLRDSEDTL QSLAHTKATL EHDLAVKANT LYIDQEKCMS MRNSY PSTL RLVGYC

UniProtKB: Tektin-3

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Macromolecule #6: Tektin-4

MacromoleculeName: Tektin-4 / type: protein_or_peptide / ID: 6 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 52.121449 KDa
SequenceString: MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETG ALAQRTQLDS TRKVGERLED MHCWKSELQR EIDELSSETD LMMAQKLRLQ RALDATSVPY SIATDNLQCR E RRQHPDLV ...String:
MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETG ALAQRTQLDS TRKVGERLED MHCWKSELQR EIDELSSETD LMMAQKLRLQ RALDATSVPY SIATDNLQCR E RRQHPDLV RDYVEVELLK ETELIRNIQE LLKRTIGQAV DQIRLNREHK ESCEMNWSDK VEVYNIDDTC SRYTNESTQV QF YPHSSKF EESASTPETW AKFNHDNLLR AERERLASVN LRKLIDCILR DTAEDLRLQC DAVNSAFSSR CQELDDSLQK LQY HLRKTL TEITDQEHQI AALKQAIKDK EAPLRVAQTR LYQRSHRPNV ELCRDNAQFR LLSEVEELNM SLRALKEKLQ DAEQ ALRNL EDSRMSLEKD IAVKTNSLFI DRQKCMTHRN RYPSVLQLAG YQ

UniProtKB: Tektin-4

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Macromolecule #7: Tektin bundle-interacting protein 1

MacromoleculeName: Tektin bundle-interacting protein 1 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.987262 KDa
SequenceString: MENVRREATR PSVPSGTLEL YFPDHLYRND YVSLEGPRWA PAIKQAVRWK FTPMGRDAAG QVWFTGLTNS EPGDAWYKLP RALDTPYRE AHTRWHGCFQ SRQRGLPPAY TQHLREMAFW DPAITAQYLN SGPRWGCMQW RDRQIRGKEF VVTRNQFGAK L PWRSDYVP ...String:
MENVRREATR PSVPSGTLEL YFPDHLYRND YVSLEGPRWA PAIKQAVRWK FTPMGRDAAG QVWFTGLTNS EPGDAWYKLP RALDTPYRE AHTRWHGCFQ SRQRGLPPAY TQHLREMAFW DPAITAQYLN SGPRWGCMQW RDRQIRGKEF VVTRNQFGAK L PWRSDYVP LLSLPQRPRF TAQDFRQRGL QRPCPAIGQP PPAFTPAL

UniProtKB: Tektin bundle-interacting protein 1

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Macromolecule #8: Tektin-5

MacromoleculeName: Tektin-5 / type: protein_or_peptide / ID: 8 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 62.817535 KDa
SequenceString: MEFLGTTQTA SFCGPKKGCG LQALPPAGQE PVVQECYQPF HLPGYRYLNA WRPSVFHKIA TSQTIPEECS GIRRPPTILP SLRSALFCR YTPRDWDRSN DLQIRNAEAS RLWASRLTGD SLRIMQDKDQ LIHQMQEGTS RNLGQRLSDL GFWKSELCYE L DRLLTENS ...String:
MEFLGTTQTA SFCGPKKGCG LQALPPAGQE PVVQECYQPF HLPGYRYLNA WRPSVFHKIA TSQTIPEECS GIRRPPTILP SLRSALFCR YTPRDWDRSN DLQIRNAEAS RLWASRLTGD SLRIMQDKDQ LIHQMQEGTS RNLGQRLSDL GFWKSELCYE L DRLLTENS SMDTLKRRLE CAAEEVNCPL QVALECLYNR EKRIGIDLVH DNVEKNLIRE VDLLKCCQDQ MRKLAKRIDF QI RDNRDAQ HSLERDIEDK SSAQYIDENC FNLRSTSDSI SFFHGVEKFD GTVSIPETWA KFSNDNIRHA QNMRANSIRL REE AEHLFE TLSDQMWKQF TNTNLAFNAR ISEETDVKNK LQTQLAKILQ EIFQAENTIM LLERAIVAKE YPLKMAQTML ACRT RRPNV ELCRDVPQFR LVNEVFTIDD TLQTLKLRLR ETQDTLQLLV MTKSRLEHEL AIKANTLCID KDKCMSMRKS FPSTP RLTG YTCSAIGSGP YANHAPRISS GPCSGSALCK GPASCGGGAS CGGGASCGGH APCGSALCSH SVSRSGPGFA PVC

UniProtKB: Tektin-5

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Macromolecule #9: EF-hand domain-containing protein 1

MacromoleculeName: EF-hand domain-containing protein 1 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 75.235422 KDa
SequenceString: MGTNPVHGLP FLPGSSFTDS TKTAFHRSQT LNYRNGYAVV RRPTMGIGGD RLHYNQLSQA ELDELANKAP ILTYGPLKQA PLAEFVPAH VAFDKKVLKF SAYFQEDVPI SMEEHYRIRH VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRFTKNDM G DHYHWKDL ...String:
MGTNPVHGLP FLPGSSFTDS TKTAFHRSQT LNYRNGYAVV RRPTMGIGGD RLHYNQLSQA ELDELANKAP ILTYGPLKQA PLAEFVPAH VAFDKKVLKF SAYFQEDVPI SMEEHYRIRH VNIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRFTKNDM G DHYHWKDL NRGINLTVYG KTFRIVDCDR FTQDFLESQG IELNPSEKIP LDPYTQLRKE PVRKYVTPSD FDQLKQFLTF DK QVLRFYA IWDDTDSLFG ECRHYIIHYY LMDDTVEIRE VHERNNGRDP FPLLMNRQRM PKVLVENAKN FPKCVLEISD QEV LEWYTA KDFIVGKPLT ILGRTFFIYD CDPFTRQFYK DKFGMPDLPP VDVTKKEPPP VKQELPPYNG YGLIEDSAQN CFAL IPKAP RKDVVKMLMN DNKVLRYLAA LESPIPEDKD RRFVFSYFLA TDMISIFEPP VRNSGIIGGK FLGRTKVVKS FSPVD NPIY YSPSDFFIGA VIEVFGHRFV ILDTDEYVLK YMESNASQYS PEALASIQNR IQKPELPAPE LESKQATGEP MVQGTE ESK VQDLDALIDQ IHMHLKYNSY KENLRETFQM YDKDESGYVD RETFFKICET LNVPVDDSLI KELIRLCTHG EGRINYY NF VRAFSN

UniProtKB: EF-hand domain-containing protein 1

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Macromolecule #10: EF-hand domain-containing family member C2

MacromoleculeName: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 87.758023 KDa
SequenceString: MALPFLPGNS FNRNIGKERF HKSQHWGFCN NVRMLVSENK PGVGGDLLYG QKIKPKHSVF PKGDGTDAPS WVAFDKQVLS FDAYLEDEI SDKRQEIFRI RYYKIYFYLE DDTIQVNEPE VINSGLPQGT SIRRQRIPYP PPNDDQFYTV YDFNINISVV F YGRTFKIY ...String:
MALPFLPGNS FNRNIGKERF HKSQHWGFCN NVRMLVSENK PGVGGDLLYG QKIKPKHSVF PKGDGTDAPS WVAFDKQVLS FDAYLEDEI SDKRQEIFRI RYYKIYFYLE DDTIQVNEPE VINSGLPQGT SIRRQRIPYP PPNDDQFYTV YDFNINISVV F YGRTFKIY DCDPFTKNFL KKIGIKLNPP GQCPLDPYMK MRRETLEFVD PFRPYQSFDT LKRFIQYDGK VLRFFCLWDD ST SLFGDRR EFVLHYFLCD GTVEIREVLP SNSGRDAMSS FLRRGKLPKY GPPGIYQPGQ ITDRAVLNVY GGLSEWRADG YLL DKYQLG KVEQDFYTDQ DLSIGATINV WGRKVLLCDC DEFTKTYYRT KYGVDNFTPI SCKPPHLPKI ERKYPPYTGF GSEE DSFRS CVGLKPTPHR KNFKKFMELD SFGNISNILR YFGKLITHKC ADVDRIFVIA FYLSDDTISV FEPIENNSGN AGGMF LKRS RVKKPGQEVF KSEFSEYIKA EELYIGATVN INGYLFILLN ADEYTLNYME NNTDKFPYSN FELAIQKLKQ EKSKSR EIT QVFAAADYNH TKVVPYNTFR DILMSITMGK LIDQELITIA RHYRVPEIMD PDLAYLIARA HEKFKKNIFE NFDMFIY NC VYEDREKKGV LPTKDIRRMC KSSRLPLDDD FLDCLLSRFE DKDHQINYEI FFSVLNWRMN PTPDLQAPPY LKEKCEDV W VGMPSPIPVK YVRYLDFLID VYGLEDNML

UniProtKB: EF-hand domain-containing family member C2

+
Macromolecule #11: Protein FAM166A

MacromoleculeName: Protein FAM166A / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 36.659566 KDa
SequenceString: MTATQKHNLF TPEPHYIPGY AGFYPQLRYQ VGNTYGRTTA QLLTDPSVQK SPCSVLSPMT KPKFIEDFSK SKPPWIPCRD LREPYIPHY TSLKPYKNFE ILGQLPRQDV DTQGPPQVEN RQGPLTAGFM PYPPYPACPP GRKGEARDLG HPGLLLAYGE E AWKDAAPL ...String:
MTATQKHNLF TPEPHYIPGY AGFYPQLRYQ VGNTYGRTTA QLLTDPSVQK SPCSVLSPMT KPKFIEDFSK SKPPWIPCRD LREPYIPHY TSLKPYKNFE ILGQLPRQDV DTQGPPQVEN RQGPLTAGFM PYPPYPACPP GRKGEARDLG HPGLLLAYGE E AWKDAAPL QDTPGKNNQL YHCRRDEYLP PHPPQETLDV GRFQRLPQLD HPNLIQRKAI SGYAGFVPRF AWVMGMNYRD GV TQAMDDF DKNQFVFRHP VCALGERLPR THWPNTTIYR SQGLIPFYMG FIPSMQDNYA LTFGNSTRRA YQKELDRRSH TL

UniProtKB: Ciliary microtubule inner protein 2A

+
Macromolecule #12: Protein FAM166C

MacromoleculeName: Protein FAM166C / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.097957 KDa
SequenceString: MAFRSAGTLM TEFNAAFVPP ALMPGYKGHV PGVASSFGSS YGNTTFKYFQ DLRNTGLEKS YALLSGGCFP TIFSPNSPLV LTDNSQNWD RWLHQPTYSR SNQDNGRTTE LTNFYKTVQQ QRKYYQDKTG ILSRVPYFVL PVKEPDRYPL PTDLPPLSEK S KWNLLRLS ...String:
MAFRSAGTLM TEFNAAFVPP ALMPGYKGHV PGVASSFGSS YGNTTFKYFQ DLRNTGLEKS YALLSGGCFP TIFSPNSPLV LTDNSQNWD RWLHQPTYSR SNQDNGRTTE LTNFYKTVQQ QRKYYQDKTG ILSRVPYFVL PVKEPDRYPL PTDLPPLSEK S KWNLLRLS PDYKRTYQTF PSGKRVSSQE RQRRDLYFEF RA

UniProtKB: Ciliary microtubule inner protein 2C

+
Macromolecule #13: Dual specificity phosphatase 21

MacromoleculeName: Dual specificity phosphatase 21 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 21.527574 KDa
SequenceString:
MTTASCIFPS QATQQDNIYG LSQITASLFI SNSAVANDKL TLSNNHITTI INVSAEVVNT FFEDIQYVQV PVSDAPNSYL YDFFDPIAD HIHGVEMRNG RTLLHCAAGV SRSATLCLAY LMKYHNMTLL DAHTWTKTCR PIIRPNNGFW EQLIHYEFKL F SRNTVRMI YSPIGLIPNI YEKEAYLMEL M

UniProtKB: Dual specificity phosphatase 21

+
Macromolecule #14: Coiled-coil domain-containing protein 105

MacromoleculeName: Coiled-coil domain-containing protein 105 / type: protein_or_peptide / ID: 14 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 57.406484 KDa
SequenceString: MPVLLPSTDP GQDSRVGAPE WRQAAKATSR KAHLLTDRCG QEAVTMWQPK DSVLDPNVAH HLGRAAYMEP WRFRVEMLKG GGTLEKPPP GEGVTLWKGK MKPPAWYARL PLPMHRDARA QQTAEVVHAH ARGARLTAAR LGRAQHQING QLRLLLRQRE A TDRRLSEV ...String:
MPVLLPSTDP GQDSRVGAPE WRQAAKATSR KAHLLTDRCG QEAVTMWQPK DSVLDPNVAH HLGRAAYMEP WRFRVEMLKG GGTLEKPPP GEGVTLWKGK MKPPAWYARL PLPMHRDARA QQTAEVVHAH ARGARLTAAR LGRAQHQING QLRLLLRQRE A TDRRLSEV RKGLLINQQS VKLRGYRPKC EKIPDKADSL LVWEKKELKS MKRKMEKDME ISEDLLKALA SCRDTLDFYC QE RLQAVEL MNQPLDKVLE QAGRHSWVDI TRPPTPRTQG LKTPPPDPIG TYTPACAKAL FEAKRLLMES KDILTEMAKN EVD IQNQQQ EISNRVCSSL AQKMRETLEL KERMTMTLGL MRGTIHRCMK FNQEMYVTRG IIKGPLLKRN LEAREKLNRP LVRM YQRHV GTQLPEATRL AQGTDLLTRH NLHMEKNLKE LRTTHDNLAW GLNCKKIGHD VDYDVVRLRL RQRHPHVCYE QAQRL VNDW DPPTPARSQT NTASK

UniProtKB: Tektin-like protein 1

+
Macromolecule #15: Enkurin

MacromoleculeName: Enkurin / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 29.587521 KDa
SequenceString: MDSPCTSESI YNLIPSDLKE PPQHPRYTSL FRATIKNDMK KFKTAMKTMG PAKVEIPSPK DFLKKHSKEK TLPPKKKFNR CSPKKPAVP LRTDHPVMGI QSGKNFINTN AADVIMGVAK KPKPIYVDKR TGDKHDLETS GLFPKYINKK DYGITPEYIC K RNEDVKKA ...String:
MDSPCTSESI YNLIPSDLKE PPQHPRYTSL FRATIKNDMK KFKTAMKTMG PAKVEIPSPK DFLKKHSKEK TLPPKKKFNR CSPKKPAVP LRTDHPVMGI QSGKNFINTN AADVIMGVAK KPKPIYVDKR TGDKHDLETS GLFPKYINKK DYGITPEYIC K RNEDVKKA QEEYDNYIQE NLKKAAMKRL SDEEREAVLQ GLKKNWEEVH KEFQSLSVFI DSVPKKIRKQ KLEKEMKQLE HD ISVIEKH KIIYIANK

UniProtKB: Enkurin

+
Macromolecule #16: Testis-expressed protein 43

MacromoleculeName: Testis-expressed protein 43 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 16.305608 KDa
SequenceString:
MASEKDDGPA LPKLDDDNQT AENTCKPAEE QPQQLRWDDI HLPRFSLKQG MIPTRYVMPW KENMKFRNVN LQQAEACGIY AGPLEDSLF WGYSERLCHG EDRKAVLKKG LPEIKITDMP LHSPLSRYQS TVISHGFRRR LI

UniProtKB: Sperm-associated microtubule inner protein 10

+
Macromolecule #17: Cilia- and flagella-associated protein 276

MacromoleculeName: Cilia- and flagella-associated protein 276 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 18.960092 KDa
SequenceString:
MLQPQETFSN PALRDEDKHL GNLWASKKSL YKNPAHLAQQ QDPWSRLSST PTATSRSRDT FFDSKIPKDD LDFRLATLYN HHTGAFKNK TEILLHQETI EDIQGTKIQF PGECFHAPSA PITSRTTIRH WINPKKESIH SIQGSIVSPH TAATNGGYSR K NDGGFFST

UniProtKB: Cilia- and flagella-associated protein 276

+
Macromolecule #18: Protein Flattop

MacromoleculeName: Protein Flattop / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.575123 KDa
SequenceString:
MATNYSANQY EKAYLPTYLQ NWSPARPTKE KIAAHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWQM PLKIPPAKVT LTARTTTAA DNLTKWIHKN PDLLNACNGL RPEISGKPFD PDSQTKQKKS VTKTVQQAPN PTIIPSSPVI QGDNPDEPQS S HPSAGHTP GPQTPVNSPN NPPPSPCKST K

UniProtKB: Protein Flattop

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Macromolecule #19: Cilia- and flagella-associated protein 52

MacromoleculeName: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 68.322164 KDa
SequenceString: MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL HGHGNNVSCV TISKEGDYIA SGQVTFMGF KADIILWDFK KRELIARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATSVVFSR ...String:
MEEQVLPELD VAELELQAVI GFNGHVPNGL KCHPDQEHLI YPLGCTVLIQ AINTNEQNFL HGHGNNVSCV TISKEGDYIA SGQVTFMGF KADIILWDFK KRELIARLSL HKGKIEALAF SPNDLYLVSL GGPDDGSVVV WSIAKRDAIC GSPAAGLNVG N ATSVVFSR CRDEMFVTAG NGTIRVWELD LPNRKIWPTE CQTGQMKRIV LSTGMADDDS FFYLGTTTGD ILKMNPKTKL LA DTGPVKD KFSLGVSALR CLKMGGLLVG SGAGLLIFCK SPSYKPIKKV QLQGGITSIT LRGEGHQFFV GTEESHIYRV NFT DFKETL IATCHFEAVQ DIVFPFGTAE LFATCAKKDI RVWHTMSKRE LLRITVPNMT CHGIDFMRDG KSIISAWDDG KIRA FAPES GRLMYTINSA HRIGVTAIAT TSDCKRIISG GGEGEVRVWQ VGCQTQKLEE ALKEHKSSVS CIRVKKNNEE CVTAS TDGT CIIWDLVRLR RNQMILANTL FQCVCYHPEE FQIITSGTDR KIAYWEVFDG SVIRELEGSL SGSINGMDIT QEGGHF VTG GHDHLVKVWD YNEGEVTHVG VGHSGNIMAM RISPGNQYIV SVSADGAILR WKYPFAS

UniProtKB: Cilia- and flagella-associated protein 52

+
Macromolecule #20: EF-hand calcium-binding domain-containing protein 6

MacromoleculeName: EF-hand calcium-binding domain-containing protein 6 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 176.028312 KDa
SequenceString: MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERI LAQKISSRRD DIKKVFQILD RNHNQMVTKG DLKRVITAFL IPLTKDQFQD LLAQIPISSL GNVPYLEFLS R FGGGIDIN ...String:
MKRNGTRLNF AKANSTKSGS TRARDLMNKM SIIPEWHSLY SNTRKLPCSR PHSSPCKMQR TIFQDLCRPS SSTTAIANPV LSYLDIERI LAQKISSRRD DIKKVFQILD RNHNQMVTKG DLKRVITAFL IPLTKDQFQD LLAQIPISSL GNVPYLEFLS R FGGGIDIN INGIKRVNEN EVDNRRTVKE VQLTEKIFRN MRSIRKVFQV MDVNNTGLVQ PQELRRVLET FCLRMQDGDY EK FLEQYNI DKTTAVDYNA FLKNLSVKND ASFKYLLSNA AELSRETQQG KNGKRLLDTG SSEDVWKNYS LDDLEKTFCQ EFS KSYEKI EKALSAGDPS KGGYISLNYL KVVLDTFIYR LPRRIFIQLI RRFGLKTSTK INWKQFLTAI YERQKLEVSK TLPL KKRSS TEARNRSRKE NIIKKLFKYS EDRYTALKKT LLIISSTPSG HITWEELRHI LNCMVAKLND SEFSELKQTF DPEGT GAVR VNSLLDVLDD STKVRKMSPS TDTKTPLPVA WDSVEELVLD SITRNLQAFY SMLQSYDLRD TGTIGKNNFR KVMRVF CPY LSNEHLVRFS SKFQEAGSGR ILYKKFLLSI GVGIPPPTPP LSSPKVQLSD QFQIEEPGQQ DERTQPSGEK TSEINNM TK EEVIDGLKHR IQQKDPVFRK QFLSISKEPD VKINQEEFRK VLERSGMPMN DCQYAMLASK LGFKNEEGMS YQDFTMGF E DCMLSGLETV PLQSRTASRT NMDEHFISAE ECLRIFPKKL KESFRDVYSA FFRIDLDRDG IISMHDFHRL LQYLQLNMV DLEFERFLSL LGLRLSVTLN FREFQNLCEK RPWKSDEAPQ RLIRCKQKVA DSELACEQAH QYLIMKAKTR WADLSKNFIE TDNEGNGIL RRRDIKNSLY GFDIPLTPRE FEKLWQNYDT EGRGYITYQE FLHRLGIRYS PKVHRPYKED YFNFLGHFTK P KQVQEEIQ ELQQISEREK LMNHYEEISK AFNAMEKSKP VALCRVQKVL QECGCPLKEE ELISLLKSLD VSVHNNHIDP VE FLRALEI SWASKARPKE KEESSPPPIS FSKVTPDEVI KTMQEVVESS QPALVEAFSA LDKEDTGFVK AMEFGDVLRS VCQ KLTDNQ YHYFLRRLRL HLTPNIHWKY FLENFSTFQD ETADDWAENM PKAPPPMSPK ETAHRDIVAR VQKAVASHYH TIVQ EFENF DTLKSNTVSR DEFRSICTRH IQILTDEQFD RLWSELPVNA KGRLKYQDFL SKLSIERVPS PPMAAGDSGE STMAQ RGSS APEFSQGTRS NLYSPPRDSR VGLKSRSHPC TPVGTPPLQN CEPIESRLRK QIQGCWRELL RECKEKDTDK QGTISA AEF LALVEKFKLD ISREESQQLI VKYDLKNNGK FAYCDFIQSC VLLLKAKETS LMRRMRIQNA DKMKEAGMET PSFYSAL LR IQPKIVHCWR PMRRSFKTYD KNGTGLLSVA DFRKVLRQYS INLSEEEFFH VLEYYDKSLS SKISYNDFLR AFLQ

UniProtKB: EF-hand calcium-binding domain-containing protein 6

+
Macromolecule #21: Cilia and flagella-associated protein 77

MacromoleculeName: Cilia and flagella-associated protein 77 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 32.78602 KDa
SequenceString: MPDPAKPGKD LTAWKKKKQP VHRTVSQICP PPRRPLTVVD IRTGMENERL GVVRDSMFQN PLIVKAELGK PRERSCSLPG INFNYGLYI RGLDGGVPEA IGHWNVFKQQ PTCPHELTRN YIAMNRGAVK AGLVTARENM LYRELNDIRI NDQEDRRQKE P PPIPPNMT ...String:
MPDPAKPGKD LTAWKKKKQP VHRTVSQICP PPRRPLTVVD IRTGMENERL GVVRDSMFQN PLIVKAELGK PRERSCSLPG INFNYGLYI RGLDGGVPEA IGHWNVFKQQ PTCPHELTRN YIAMNRGAVK AGLVTARENM LYRELNDIRI NDQEDRRQKE P PPIPPNMT FGIRSRPSTP FFDLLQHRYQ QLWVQEQKAT QQAIKMEKKQ KVILGKLYET RSSQLRKYKP PVKLDALWHM PH FKKVASH LATFPTEADR QRALKAHKEE YAVRQGTLRM GNYTHP

UniProtKB: Cilia- and flagella-associated protein 77

+
Macromolecule #22: Cilia- and flagella-associated protein 20

MacromoleculeName: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 22 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 22.781389 KDa
SequenceString: MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS ...String:
MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia- and flagella-associated protein 20

+
Macromolecule #23: Parkin coregulated gene protein homolog

MacromoleculeName: Parkin coregulated gene protein homolog / type: protein_or_peptide / ID: 23 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 27.763268 KDa
SequenceString: MPKRTKLLPQ QTFQVHQPRS LVSEGFTVKA MMKNSVVRGP PVAGAFKERP AKPTTFRKCY ERGDFPIALE HDSKGNKIAW KVEIEKLDY HHYLPLFFDG LSEMTFPYEF FARRGIHDML EHGGNKILPV IPQLIIPIKN ALNLRNRQII CVTLKVLQHL V VSSEMVGE ...String:
MPKRTKLLPQ QTFQVHQPRS LVSEGFTVKA MMKNSVVRGP PVAGAFKERP AKPTTFRKCY ERGDFPIALE HDSKGNKIAW KVEIEKLDY HHYLPLFFDG LSEMTFPYEF FARRGIHDML EHGGNKILPV IPQLIIPIKN ALNLRNRQII CVTLKVLQHL V VSSEMVGE ALLPYYRQIL PILNIFKNMN VNSGDGIDYS QQKRENIGDL IQETLEAFER YGGEDAFINI KYMVPTYESC LL N

UniProtKB: Parkin coregulated gene protein homolog

+
Macromolecule #24: Sperm acrosome-associated protein 9

MacromoleculeName: Sperm acrosome-associated protein 9 / type: protein_or_peptide / ID: 24 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 19.512373 KDa
SequenceString:
MNEVKESLRS IEQKYKLFQQ QQFTFIAALE HCRENAHDKI RPISSIEQVQ SYMEHYCNNS THRRILIMFM DICSELSKLC QHFEALHSG TPVTNSLLEK CKTLVSQSND LSSLRAKYPH EVVNHLSCDE ARNHYGGVVS LIPIVLDFMK EWIAHSEKLP R KVLQHGTT

UniProtKB: Sperm acrosome-associated protein 9

+
Macromolecule #25: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 25 / Number of copies: 123 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

-
Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 3.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 37018
ExtractionNumber tomograms: 689 / Number images used: 37018
Final angle assignmentType: MAXIMUM LIKELIHOOD

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