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-Structure paper
タイトル | In-cell structural insight into the stability of sperm microtubule doublet. |
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ジャーナル・号・ページ | Cell Discov, Vol. 9, Issue 1, Page 116, Year 2023 |
掲載日 | 2023年11月21日 |
著者 | Linhua Tai / Guoliang Yin / Xiaojun Huang / Fei Sun / Yun Zhu / |
PubMed 要旨 | The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. ...The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of intact sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT at 4.5-7.5 Å resolutions, and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of intact sperm DMT in 48 nm periodicity that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies. |
リンク | Cell Discov / PubMed:37989994 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 4.5 - 14.08 Å |
構造データ | EMDB-35210: In situ structure of A-tubule of axonemal doublet microtubules in mouse sperm with 16-nm repeat EMDB-35211: In situ structure of B-tubule of axonemal doublet microtubules in mouse sperm with 16-nm repeat EMDB-35222: A1 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat EMDB-35224: A2 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat EMDB-35225: A3 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat EMDB-35226: B1 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat EMDB-35227: B2 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat EMDB-35228: B3 part of axonemal doublet microtubules in mouse sperm with 48-nm repeat EMDB-35229, PDB-8i7o: EMDB-35230, PDB-8i7r: EMDB-35231: Half of axonemal doublet microtubules in mouse sperm with 96-nm repeat EMDB-35232: Half of axonemal doublet microtubules in mouse sperm with 96-nm repeat EMDB-35236: Axonemal doublet microtubules in mouse sperm with 96-nm repeat EMDB-35237: Axonemal doublet microtubules in deformed mouse sperm with 16-nm repeat EMDB-35238: Axonemal doublet microtubules in deformed mouse sperm with 48-nm repeat EMDB-37643: Consensus map of axonemal doublet microtubules in mouse sperm with 96-nm repeat |
化合物 | ChemComp-GTP: |
由来 |
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キーワード | STRUCTURAL PROTEIN / microtubules / axoneme / sperm / filament |