Francesca Vallese / Kookjoo Kim / Laura Y Yen / Jake D Johnston / Alex J Noble / Tito Calì / Oliver Biggs Clarke /
PubMed Abstract
The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association ...The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association with the band 3 anion exchanger and the Rhesus glycoproteins remains unknown. Here we present structures of ankyrin-1 complexes purified from human erythrocytes. We reveal the architecture of a core complex of ankyrin-1, the Rhesus proteins RhAG and RhCE, the band 3 anion exchanger, protein 4.2, glycophorin A and glycophorin B. The distinct T-shaped conformation of membrane-bound ankyrin-1 facilitates recognition of RhCE and, unexpectedly, the water channel aquaporin-1. Together, our results uncover the molecular details of ankyrin-1 association with the erythrocyte membrane, and illustrate the mechanism of ankyrin-mediated membrane protein clustering.
EMDB-26886, PDB-7uze: Erythrocyte ankyrin-1 complex class 2 local refinement of AQP1 (C4 symmetry applied) Method: EM (single particle) / Resolution: 2.4 Å
EMDB-26916, PDB-7uzq: Local refinement of RhAG-RhCE-ANK1(AR1-5), from consensus refinement of all classes Method: EM (single particle) / Resolution: 2.17 Å
EMDB-26917, PDB-7uzs: Protein 4.2 (local refinement from consensus reconstruction of ankyrin complex classes) Method: EM (single particle) / Resolution: 2.2 Å
EMDB-26918, PDB-7uzu: Ankyrin-1 (N-terminal region of membrane binding domain, local refinement from consensus reconstruction; bound to N-terminal peptide from band 3) Method: EM (single particle) / Resolution: 2.3 Å
EMDB-26919, PDB-7uzv: Cytoplasmic domains of Band 3-I (local refinement from consensus reconstruction of ankyrin complexes) Method: EM (single particle) / Resolution: 2.5 Å
EMDB-26940, PDB-7v07: Band 3-I-TM local refinement from erythrocyte ankyrin-1 complex consensus reconstruction Method: EM (single particle) / Resolution: 2.8 Å
EMDB-26944, PDB-7v0m: Local refinement of ankyrin-1 (N-terminal half), class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 2.7 Å
EMDB-26950, PDB-7v0t: Local refinement of Band 3-I cytoplasmic domains, class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 2.7 Å
EMDB-26951, PDB-7v0u: Local refinement of Band 3-II cytoplasmic domains, class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.0 Å
EMDB-26952, PDB-7v0x: Local refinement of ankyrin-1 (C-terminal half), class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.0 Å
EMDB-26953, PDB-7v0y: Local refinement of Band 3-III cytoplasmic domains, class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.0 Å
EMDB-26954, PDB-7v19: Local refinement of Band 3-II transmembrane domains, class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.3 Å
EMDB-26955, PDB-8crq: Local refinement of Band 3-I transmembrane domains, class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.2 Å
EMDB-26956, PDB-8crr: Local refinement of Band 3-III transmembrane domains, class 1 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.0 Å
EMDB-26958, PDB-8crt: Local refinement of Rh trimer, glycophorin B and Band3-III transmembrane region, class 1a of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.0 Å
EMDB-26972, PDB-8csv: Local refinement of Anykyrin-1 (N-terminal half of membrane binding domain) in Class 2 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 2.7 Å
EMDB-26975, PDB-8csy: Local refinement of cytoplasmic domains of band3-I in class 2 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 2.7 Å
EMDB-26978, PDB-8ct2: Local refinement of AQP1 tetramer (C1; refinement mask included D1 of protein 4.2 and Ankyrin-1 AR1-5) in Class 2 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.1 Å
EMDB-26979, PDB-8ct3: Local refinement of band3-I transmembrane region from class 2 of erythrocyte ankyrin-1 complex Method: EM (single particle) / Resolution: 3.3 Å
TRANSPORT PROTEIN / Membrane Protein / Anion Exchange / Erythrocyte / Glycoprotein / STRUCTURAL PROTEIN / Ankyrin / TRANSPORT PROTEIN/STRUCTURAL PROTEIN / TRANSPORT PROTEIN-STRUCTURAL PROTEIN complex / ankyrin complex
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Keywords
homo sapiens (human)