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-Structure paper
タイトル | High-resolution structural-omics of human liver enzymes. |
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ジャーナル・号・ページ | Cell Rep, Vol. 42, Issue 6, Page 112609, Year 2023 |
掲載日 | 2023年6月27日 |
著者 | Chih-Chia Su / Meinan Lyu / Zhemin Zhang / Masaru Miyagi / Wei Huang / Derek J Taylor / Edward W Yu / |
PubMed 要旨 | We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined ...We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined high-resolution structural information for ten unique human liver enzymes involved in diverse cellular processes. Notably, we determined the structure of the endoplasmic bifunctional protein H6PD, where the N- and C-terminal domains independently possess glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase enzymatic activity, respectively. We also obtained the structure of heterodimeric human GANAB, an ER glycoprotein quality-control machinery that contains a catalytic α subunit and a noncatalytic β subunit. In addition, we observed a decameric peroxidase, PRDX4, which directly contacts a disulfide isomerase-related protein, ERp46. Structural data suggest that several glycosylations, bound endogenous compounds, and ions associate with these human liver enzymes. These results highlight the importance of cryo-EM in facilitating the elucidation of human organ proteomics at the atomic level. |
リンク | Cell Rep / PubMed:37289586 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.64 - 3.47 Å |
構造データ | EMDB-26915, PDB-7uzm: EMDB-28214, PDB-8ekw: EMDB-28217, PDB-8eky: EMDB-28232, PDB-8em2: EMDB-28262, PDB-8emr: EMDB-28263, PDB-8ems: EMDB-28264, PDB-8emt: EMDB-28271, PDB-8ene: EMDB-28377, PDB-8eoj: EMDB-28465, PDB-8eor: |
化合物 | ChemComp-HOH: ChemComp-NAG: ChemComp-CA: ChemComp-PLP: ChemComp-FES: ChemComp-MTE: ChemComp-MOS: ChemComp-FAD: ChemComp-NAD: ChemComp-EEE: |
由来 |
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キーワード | OXIDOREDUCTASE / aldehyde dehydrogenase / human liver / Peroxiredoxin-4 / H6PD / HYDROLASE / glucosidase II / GANAB / glycosyl hydrolase 31 family / Glycogen Phosphorylase / PLP / Aldehyde oxidase / AOX1 / TRANSPORT PROTEIN / Microsomal triglyceride transfer protein / LIPID TRANSPORT / ISOMERASE / carboxylesterase |