EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism for the initiation of spliceosome disassembly.
ジャーナル・号・ページ	Nature, Vol. 632, Issue 8024, Page 443-450, Year 2024
掲載日	2024年6月26日
著者	Matthias K Vorländer / Patricia Rothe / Justus Kleifeld / Eric D Cormack / Lalitha Veleti / Daria Riabov-Bassat / Laura Fin / Alex W Phillips / Luisa Cochella / Clemens Plaschka /
PubMed 要旨	Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome. Recent studies have shed light on ...Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome. Recent studies have shed light on spliceosome assembly and remodelling for catalysis, but the mechanism of disassembly remains unclear. Here we report cryo-electron microscopy structures of nematode and human terminal intron lariat spliceosomes along with biochemical and genetic data. Our results uncover how four disassembly factors and the conserved RNA helicase DHX15 initiate spliceosome disassembly. The disassembly factors probe large inner and outer spliceosome surfaces to detect the release of ligated mRNA. Two of these factors, TFIP11 and C19L1, and three general spliceosome subunits, SYF1, SYF2 and SDE2, then dock and activate DHX15 on the catalytic U6 snRNA to initiate disassembly. U6 therefore controls both the start and end of pre-mRNA splicing. Taken together, our results explain the molecular basis of the initiation of canonical spliceosome disassembly and provide a framework to understand general spliceosomal RNA helicase control and the discard of aberrant spliceosomes.
リンク	Nature / PubMed:38925148 / PubMed Central
手法	EM (単粒子)
解像度	2.73 - 69968.0 Å
構造データ	19397 8ro0 EMDB-19397: Composite map of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS') PDB-8ro0: Structure of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS') 手法: EM (単粒子) / 解像度: 2.9 Å 19398 8ro1 EMDB-19398, PDB-8ro1: Structure of the C. elegans Intron Lariat Spliceosome double-primed for disassembly (ILS'') 手法: EM (単粒子) / 解像度: 3.0 Å 19399 8ro2 EMDB-19399, PDB-8ro2: Integrative Structure of the human intron lariat Spliceosome (ILS'') 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-50447: Structure of the C. elegans Intron Lariat Spliceosome (Map 1) 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-50449: Structure of the C. elegans Intron Lariat Spliceosome (Map 2) 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-50450: Structure of the C. elegans Intron Lariat Spliceosome (Map 3) 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-50451: Structure of the C. elegans Intron Lariat Spliceosome (Map 4) 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-50452: Structure of the C. elegans Intron Lariat Spliceosome (Map 5) 手法: EM (単粒子) / 解像度: 5.51 Å EMDB-50453: Structure of the C. elegans Intron Lariat Spliceosome (Map 6) 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-50454: Structure of the C. elegans Intron Lariat Spliceosome (Map 7) 手法: EM (単粒子) / 解像度: 6.35 Å EMDB-50455: Structure of the C. elegans Intron Lariat Spliceosome (Map 8) 手法: EM (単粒子) / 解像度: 3.09 Å EMDB-50456: Structure of the C. elegans Intron Lariat Spliceosome (Map 9) 手法: EM (単粒子) / 解像度: 3.69 Å EMDB-50457: Structure of the C. elegans Intron Lariat Spliceosome (Map 10) 手法: EM (単粒子) / 解像度: 2.73 Å EMDB-50458: Structure of the C. elegans Intron Lariat Spliceosome (Map 11) 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-50459: Structure of the C. elegans Intron Lariat Spliceosome (Map 12) 手法: EM (単粒子) / 解像度: 4.77 Å EMDB-50460: Structure of the C. elegans Intron Lariat Spliceosome (Map 13) 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-50461: Structure of the C. elegans Intron Lariat Spliceosome (Map 14) 手法: EM (単粒子) / 解像度: 4.24 Å EMDB-50462: Structure of the C. elegans Intron Lariat Spliceosome (Map 15) 手法: EM (単粒子) / 解像度: 3.61 Å EMDB-50463: Structure of the C. elegans Intron Lariat Spliceosome (Map 16) 手法: EM (単粒子) / 解像度: 3.06 Å EMDB-50464: Structure of the C. elegans Intron Lariat Spliceosome (Map 17) 手法: EM (単粒子) / 解像度: 3.06 Å EMDB-50465: Structure of the C. elegans Intron Lariat Spliceosome (Map 18) 手法: EM (単粒子) / 解像度: 2.82 Å EMDB-50466: Structure of the C. elegans Intron Lariat Spliceosome (Map 19) 手法: EM (単粒子) / 解像度: 3.14 Å EMDB-50467: Structure of the C. elegans Intron Lariat Spliceosome (Map 20) 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-50468: Structure of the C. elegans Intron Lariat Spliceosome (Map 21) 手法: EM (単粒子) / 解像度: 6.33 Å EMDB-50469: Structure of the C. elegans Intron Lariat Spliceosome (Map 22) 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-50471: Structure of the C. elegans Intron Lariat Spliceosome (Map 23) 手法: EM (単粒子) / 解像度: 3.62 Å EMDB-50472: Structure of the C. elegans Intron Lariat Spliceosome (Map 24) 手法: EM (単粒子) / 解像度: 69968.0 Å EMDB-50473: Structure of the C. elegans Intron Lariat Spliceosome (Map 25) 手法: EM (単粒子) / 解像度: 3.94 Å EMDB-50474: Structure of the C. elegans Intron Lariat Spliceosome (Map 27) 手法: EM (単粒子) / 解像度: 4.19 Å EMDB-50475: Structure of the C. elegans Intron Lariat Spliceosome (Map 26) 手法: EM (単粒子) / 解像度: 3.91 Å PDB-9fmd: Integrative model of the human post-catalytic spliceosome (P-complex) 手法: ELECTRON MICROSCOPY / 解像度: 3.3 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / フィチン酸 ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-ZN: Unknown entry ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM
由来	caenorhabditis elegans (センチュウ) homo sapiens (ヒト) human adenovirus 2 (ヒトアデノウイルス)
キーワード	SPLICING / mRNA / Intorn Lariat spliceosome / ILS / pre-mRNA / pre-mRNA splicing / intron lariat spliceosome / gene expression / spliceosome / P-complex