EMDB-19397: Composite map of the C. elegans Intron Lariat Spliceosome primed ...

Basic information

Entry

Database: EMDB / ID: EMD-19397

• Title: Composite map of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')
• Map data: Composite Map

Sample

• Complex: Intron lariat spliceosome
  • RNA: x 3 types
  • Protein or peptide: x 32 types
• DNA: x 1 types
• Ligand: x 4 types

• Keywords: mRNA / splicing / Intorn Lariat spliceosome / ILS / pre-mRNA

Function / homology

Function:

feminization of hermaphroditic germ-line / molting cycle / regulation of primary miRNA processing / SLBP independent Processing of Histone Pre-mRNAs / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Transport of Mature mRNA derived from an Intron-Containing Transcript / snRNP Assembly / Downregulation of SMAD2/3:SMAD4 transcriptional activity / mRNA Splicing - Minor Pathway / germline cell cycle switching, mitotic to meiotic cell cycle / mRNA Splicing - Major Pathway / mRNA 3'-end processing / RNA Polymerase II Transcription Termination / vulval development / nematode larval development / egg-laying behavior / post-spliceosomal complex / U2-type post-mRNA release spliceosomal complex / spliceosomal complex disassembly / snRNP binding / post-mRNA release spliceosomal complex / apoptotic DNA fragmentation / nuclear mRNA surveillance / generation of catalytic spliceosome for first transesterification step / nuclease activity / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / U12-type spliceosomal complex / Prp19 complex / pICln-Sm protein complex / pre-mRNA binding / U2-type catalytic step 1 spliceosome / SMN-Sm protein complex / locomotion / spliceosomal tri-snRNP complex / P granule / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / commitment complex / U2-type catalytic step 2 spliceosome / embryo development ending in birth or egg hatching / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / cyclosporin A binding / precatalytic spliceosome / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / germ cell development / uterus development / protein K63-linked ubiquitination / mRNA 3'-splice site recognition / spliceosomal tri-snRNP complex assembly / U5 snRNA binding / U5 snRNP / U2 snRNA binding / U6 snRNA binding / spliceosomal snRNP assembly / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / RNA splicing / helicase activity / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / mRNA processing / ubiquitin-protein transferase activity / metallopeptidase activity / ubiquitin protein ligase activity / protein folding / regulation of gene expression / nucleic acid binding / RNA helicase activity / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA helicase / cell division / intracellular membrane-bounded organelle / DNA repair / GTPase activity / mRNA binding / apoptotic process / regulation of transcription by RNA polymerase II / GTP binding / positive regulation of DNA-templated transcription / ATP hydrolysis activity / DNA binding / RNA binding / nucleoplasm / ATP binding / nucleus / metal ion binding

Domain/homology:

Intron Large complex component GCFC2-like / Tuftelin interacting protein, N-terminal domain / GCF, C-terminal / Septin and tuftelin interacting protein / TFP11/STIP/Ntr1 / GC-rich sequence DNA-binding factor-like protein / Tuftelin interacting protein N terminal / mRNA splicing factor Cwf18-like / : / cwf18 pre-mRNA splicing factor / Nineteen complex-related protein 2 / : / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / : / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Pre-mRNA-splicing factor SPF27 / Torus domain / Breast carcinoma amplified sequence 2 (BCAS2) / Torus domain / mRNA splicing factor SYF2 / SYF2 splicing factor / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / Peptidyl-prolyl cis-trans isomerase E / Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif / Myb-like domain profile. / Helix hairpin bin domain superfamily / G-patch domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / G-patch domain profile. / Pre-mRNA-processing factor 17 / G-patch domain / glycine rich nucleic binding domain / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / : / STL11, N-terminal / U-box domain / : / DNA2/NAM7 helicase, helicase domain / DNA2/NAM7-like helicase / AAA domain / WD repeat Prp46/PLRG1-like / BUD31/G10-related, conserved site / : / : / : / G10 protein signature 1. / G10 protein signature 2. / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Myb-like DNA-binding domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / HAT (Half-A-TPR) repeat / Cwf15/Cwc15 cell cycle control protein / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / Small nuclear ribonucleoprotein D1 / U-box domain profile. / Modified RING finger domain / U-box domain / Zinc finger, CCCH-type superfamily / Leucine-rich repeat / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / zinc finger / Pre-mRNA-splicing factor Syf1-like / Sec63 Brl domain / Snu114, GTP-binding domain / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Small nuclear ribonucleoprotein Sm D3 / Sec63 domain / Small nuclear ribonucleoprotein Sm D2 / Sec63 Brl domain / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Myb-type HTH DNA-binding domain profile. / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type

Component:

Small nuclear ribonucleoprotein Sm D1 / GCF C-terminal domain-containing protein / WD_REPEATS_REGION domain-containing protein / Cell division cycle 5-like protein / TPR_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / Spliceosome-associated protein CWC15 homolog / Protein BUD31 homolog / Pre-mRNA-splicing factor 8 homolog / Probable small nuclear ribonucleoprotein F / Pre-mRNA-splicing factor SYF1 / Probable small nuclear ribonucleoprotein-associated protein B / Pre-mRNA-splicing factor syf-2 / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein Sm D3 / Septin and tuftelin-interacting protein 1 homolog / Peptidyl-prolyl cis-trans isomerase / Probable small nuclear ribonucleoprotein Sm D2 / WD_REPEATS_REGION domain-containing protein / Protein isy-1 / RRM domain-containing protein / Pre-mRNA-splicing factor RBM22 / Pre-mRNA-splicing factor SPF27 / Uncharacterized protein T27F2.1 / Tr-type G domain-containing protein / Coiled-coil domain-containing protein 12 / Probable U2 small nuclear ribonucleoprotein A' / Probable small nuclear ribonucleoprotein G / Intron-binding protein aquarius / U5 small nuclear ribonucleoprotein 200 kDa helicase / Peptidyl-prolyl cis-trans isomerase E / Probable small nuclear ribonucleoprotein E

• Biological species: Caenorhabditis elegans (invertebrata)
• Method: single particle reconstruction / cryo EM / Resolution: 2.9 Å
• Authors: Vorlaender MK / Rothe P / Plaschka C

Funding support

European Union, 1 items

Organization	Grant number	Country
European Research Council (ERC)		European Union

• Citation: Journal: Nature / Year: 2024; Title: Mechanism for the initiation of spliceosome disassembly.; Authors: Matthias K Vorländer / Patricia Rothe / Justus Kleifeld / Eric D Cormack / Lalitha Veleti / Daria Riabov-Bassat / Laura Fin / Alex W Phillips / Luisa Cochella / Clemens Plaschka / ; Abstract: Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome. Recent studies have shed light on spliceosome assembly and remodelling for catalysis, but the mechanism of disassembly remains unclear. Here we report cryo-electron microscopy structures of nematode and human terminal intron lariat spliceosomes along with biochemical and genetic data. Our results uncover how four disassembly factors and the conserved RNA helicase DHX15 initiate spliceosome disassembly. The disassembly factors probe large inner and outer spliceosome surfaces to detect the release of ligated mRNA. Two of these factors, TFIP11 and C19L1, and three general spliceosome subunits, SYF1, SYF2 and SDE2, then dock and activate DHX15 on the catalytic U6 snRNA to initiate disassembly. U6 therefore controls both the start and end of pre-mRNA splicing. Taken together, our results explain the molecular basis of the initiation of canonical spliceosome disassembly and provide a framework to understand general spliceosomal RNA helicase control and the discard of aberrant spliceosomes.

History

Deposition	Jan 11, 2024	-
Header (metadata) release	Aug 7, 2024	-
Map release	Aug 7, 2024	-
Update	Aug 7, 2024	-
Current status	Aug 7, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_19397.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Composite Map
• Voxel size: X=Y=Z: 1.3013 Å

Density

Contour Level	By AUTHOR: 0.5
Minimum - Maximum	0.0 - 8.985284
Average (Standard dev.)	0.00885728 (±0.093956225)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 448 448 448 Spacing 448 448 448
Cell	A=B=C: 582.9824 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Intron lariat spliceosome

• Entire: Name: Intron lariat spliceosome

Components

• Complex: Intron lariat spliceosome
  • RNA: U2 snRNA
  • RNA: U5 snRNA
  • RNA: U6 snRNA
  • Protein or peptide: Pre-mRNA-splicing factor 8 homolog
  • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
  • Protein or peptide: Tr-type G domain-containing protein
  • Protein or peptide: Protein isy-1
  • Protein or peptide: WD_REPEATS_REGION domain-containing protein
  • Protein or peptide: Pre-mRNA-splicing factor SYF1
  • Protein or peptide: TPR_REGION domain-containing protein
  • Protein or peptide: Pre-mRNA-splicing factor SPF27
  • Protein or peptide: Cell division cycle 5-like protein
  • Protein or peptide: Pre-mRNA-splicing factor syf-2
  • Protein or peptide: Protein BUD31 homolog
  • Protein or peptide: Pre-mRNA-splicing factor RBM22
  • Protein or peptide: Spliceosome-associated protein CWC15 homolog
  • Protein or peptide: GCF C-terminal domain-containing protein
  • Protein or peptide: Intron-binding protein aquarius
  • Protein or peptide: Uncharacterized protein T27F2.1
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase
  • Protein or peptide: WD_REPEATS_REGION domain-containing protein
  • Protein or peptide: Septin and tuftelin-interacting protein 1 homolog
  • Protein or peptide: WD_REPEATS_REGION domain-containing protein
  • Protein or peptide: Coiled-coil domain-containing protein 12
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Probable small nuclear ribonucleoprotein-associated protein B
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: Probable small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: Probable small nuclear ribonucleoprotein E
  • Protein or peptide: Probable small nuclear ribonucleoprotein F
  • Protein or peptide: Probable small nuclear ribonucleoprotein G
  • Protein or peptide: Probable U2 small nuclear ribonucleoprotein A'
  • Protein or peptide: RRM domain-containing protein
  • Protein or peptide: Pre-mRNA-processing factor 19
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase E
• DNA: Intron lariat RNA
• Ligand: MAGNESIUM ION
• Ligand: INOSITOL HEXAKISPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: Intron lariat spliceosome

• Supramolecule: Name: Intron lariat spliceosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 ; Details: Full sequence: AUCGCUUCUUCGGCUUAUUAGCUAAGAUCAAAGUGUAGUAUCUGUUCUUAUCGUAUUAAC CUACGGUAUACACUCGAAUGAGUGUAAUAAAGGUUAUAUGAUUUUUGGAACCUAGGGAAG ACUCGGGGCUUGCUCCGACUUCCCAAGGGUCGUCCUGGCGUUGCACUGCUGCCGGGCUCGGCCCAGUCCCC; Number of copies: 1
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 56.554492 KDa

Sequence

String:

AUCGCUUCUU CGGCUUAUUA GCUAAGAUCA AAGUGUAGUA UCUGUUCUUA UCGUAUUAAC CUACGGUAUA CACUCGAAUG AGUGUAAUA AAGGUUAUAU GAUUUUUGGA ACCUAGGGAA GACUCGGGGC UUGCUCCGAC UUCCCAAGGG (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)U CCCC

GENBANK: GENBANK: X51372.1, GENBANK: X51372.1

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 38.680836 KDa

Sequence

String:

AAACUCUGGU UCCUCUGCAU UUAACCGUGA AAAUCUUUCG CCUUUUACUA AAGAUUUCCG UGCAAAGGAG CAUACAUUGA GUAUUAUAU ACAAUUUUUG GAGUCCCCUC GAAAGAGCGG GA

GENBANK: GENBANK: Z69659.1

Macromolecule #3: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 32.483355 KDa

Sequence

String:

GUUCUUCCGA GAACAUAUAC UAAAAUUGGA ACAAUACAGA GAAGAUUAGC AUGGCCCCUG CGCAAGGAUG ACACGCAAAU UCGUGAAGC GUUCCAAAUU UU

GENBANK: GENBANK: X51387.1

Macromolecule #4: Pre-mRNA-splicing factor 8 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor 8 homolog / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 272.396156 KDa

Sequence

String:

MANYGGHPQT EPHAIPDSIL EEKSRKWKQL QGKRYSEKKK FGMSDTQKEE MPPEHVRKVI RDHGDMTSRK YRHDKRVYLG ALKYMPHAV LKLLENMPMP WEQIRDVKVL YHITGAITFV NDIPRVIEPV YMAQWGTMWI MMRREKRDRR HFKRMRFPPF D DEEPPLDY ADNILDVEPL EPIQMELDPE EDGAVAEWFY DHKPLATTRF VNGPTYRKWA FSIPQMSTLY RLANQLLTDL VD DNYFYLF DMKSFFTAKA LNVAIPGGPK FEPLVKDLHT DEDWNEFNDI NKVIIRAPIR TEYRIAFPFM YNNLISSLPV QVS WYHTPS VVFIKTEDPD LPAFYYDPLI NPIVLSNLKA TEENLPEGEE EDEWELPEDV RPIFEDVPLY TDNTANGLAL LWAP RPFNL RSGRTRRAVD VPLVKSWYRE HCPAGMPVKV RVSYQKLLKV FVLNALKHRP PKPQKRRYLF RSFKATKFFQ TTTLD WVEA GLQVLRQGYN MLNLLIHRKN LNYLHLDYNF NLKPVKTLTT KERKKSRFGN AFHLCREILR LTKLVVDAHV QYRLNN VDA YQLADGLQYI FAHVGQLTGM YRYKYKLMRQ VRMCKDLKHL IYYRFNTGPV GKGPGCGFWA PGWRVWLFFL RGITPLL ER WLGNLLSRQF EGRHSKGVAK TVTKQRVESH FDLELRAAVM HDILDMMPDG IKQNKARVIL QHLSEAWRCW KANIPWKV P GLPTPVENMI LRYVKAKADW WTNSAHYNRE RVRRGATVDK TVCKKNLGRL TRLYLKSEQE RQHNYLKDGP YISAEEAVA IYTTTVHWLE SRRFSPIPFP PLSYKHDTKL LILALERLKE SYSVKNRLNQ SQREELALIE QAYDNPHEAL SRIKRHMLTQ RAFKEVGIE FMDLYTHLIP VYDIEPLEKV TDAYLDQYLW YEADKRRLFP AWVKPGDTEP PPLLTYKWCQ GLNNLQDVWE T SEGECNVI METKLEKIAE KMDLTLLNRL LRLIVDHNIA DYMTSKNNVL INYKDMNHTN SFGIIRGLQF ASFIVQFYGL VL DLLVLGL RRASEIAGPP QCPNEFLQFQ DVATEIGHPI RLYCRYIDRV WIMFRFSADE ARDLIQRYLT EHPDPNNENI VGY NNKKCW PRDARMRLMK HDVNLGRAVF WDIKNRLPRS ITTVEWENSF VSVYSKDNPN MLFDMSGFEC RILPKCRTAN EEFV HRDGV WNLQNEVTKE RTAQCFLKVD EESLSKFHNR IRQILMSSGS TTFTKIVNKW NTALIGLMTY FREAVVNTQE LLDLL VKCE NKIQTRIKIG LNSKMPSRFP PVVFYTPKEI GGLGMLSMGH VLIPQSDLRW MQQTEAGGVT HFRSGMSHDE DQLIPN LYR YIQPWEAEFV DSVRVWAEYA LKRQEANAQN RRLTLEDLDD SWDRGIPRIN TLFQKDRHTL AYDKGWRVRT EFKAYQI LK QNPFWWTHQR HDGKLWNLNN YRTDMIQALG GVEGILEHTL FRGTYFPTWE GLFWERASGF EESMKFKKLT NAQRSGLN Q IPNRRFTLWW SPTINRANVY VGFQVQLDLT GIFMHGKIPT LKISLIQIFR AHLWQKIHES VVMDLCQVFD QELDALEIQ TVQKETIHPR KSYKMNSSCA DVLLFAQYKW NVSRPSLMAD SKDVMDNTTT QKYWLDVQLR WGDYDSHDVE RYARAKFLDY TTDNMSIYP SPTGVLIAID LAYNLYSAYG NWFPGMKPLI RQAMAKIIKA NPAFYVLRER IRKGLQLYSS EPTEPYLTSQ N YGELFSNQ IIWFVDDTNV YRVTIHKTFE GNLTTKPING AIFIFNPRTG QLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV AA LIRSLPV EEQPRQIIVT RKAMLDPLEV HLLDFPNIVI KGSELMLPFQ AIMKVEKFGD LILKATEPQM VLFNLYDDWL KTI SSYTAF SRVVLIMRGM HINPDKTKVI LKPDKTTITE PHHIWPTLSD DDWIKVELAL KDMILADYGK KNNVNVASLT QSEV RDIIL GMEISAPSQQ RQQIADIEKQ TKEQSQVTAT TTRTVNKHGD EIITATTSNY ETASFASRTE WRVRAISSTN LHLRT QHIY VNSDDVKDTG YTYILPKNIL KKFITISDLR TQIAGFMYGV SPPDNPQVKE IRCIVLVPQT GSHQQVNLPT QLPDHE LLR DFEPLGWMHT QPNELPQLSP QDVTTHAKLL TDNISWDGEK TVMITCSFTP GSVSLTAYKL TPSGYEWGKA NTDKGNN PK GYMPTHYEKV QMLLSDRFLG YFMVPSNGVW NYNFQGQRWS PAMKFDVCLS NPKEYYHEDH RPVHFHNFKA FDDPLGTG S ADREDAFA

UniProtKB: Pre-mRNA-splicing factor 8 homolog

Macromolecule #5: U5 small nuclear ribonucleoprotein 200 kDa helicase

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 244.151594 KDa

Sequence

String:

MADELARIQQ YEYRQNSNLV LSVDYNLTDR RGREEPTGEV LPITDKEMRK MKMGDRAIKG KAPVQDQKKK RKKKDDEKAQ QFGRNVLVD NNELMGAYKP RTQETKQTYE VILSFILDAL GDVPREVLCG AADEVLLTLK NDKFRDKEKK KEVEALLGPL T DDRIAVLI NLSKKISDFS IEEENKPEGD GDIYENEGVN VQFDSDEEED DGGMVNEIKG DSEEESEEEE GVDTDYTATL KG DGHLTED EQKARGILHP RDIDAHWIQR SLAKYFKDPL IAQQKQTEVI GILKNAADDR DAENQLVLLL GFDQFEFIKC LRQ NRLMIL YCTLLRQANE KERLQIEDDM RSRPELHPIL ALLQETDEGS VVQVEKSKRD AEKSKKAATA ANEAISAGQW QAGR KMLDL NDLTFSQGSH LMSNKRCELP DGSYRRQKKS YEEIHVPALK PRPFAEGEKL VSVSELPKWA QPAFDGYKSL NRIQS RLCD SALRSKEHLL LCAPTGAGKT NVALLTMLQE IGNHLAEDGS VKLDEFKIVY IAPMKSLVQE MVGSFSKRLA PFGITV GEM TGDAQMSKEQ FMATQVIVCT PEKYDVVTRK GGERAYNQMV RLLIIDEIHL LHDDRGPVLE SIVVRTIRQM EQNHDEC RL VGLSATLPNY QDVATFLRVK PEHLHFFDNS YRPVPLEQQY IGVTEKKALK RFQAMNEVVY DKIMEHAGKS QVLVFVHS R KETAKTAKAI RDACLEKDTL SAFMREGSAS TEILRTEAEQ AKNLDLKDLL PYGFAIHHAG MNRVDRTLVE DLFADRHIQ VLFSTATLAW GVNLPAHTVI IKGTQIYNPE KGRWTELGAL DIMQMLGRAG RPQYDDRGEG ILITNHSELQ YYLSLMNQQL PVESQMVSR LTDMLNAEVV LGTVSSVSEA TNWLGYTFLF VRMLKNPTLY GITHEQARAD PLLEQRRADL IHTACVLLDK A GLIKYDKR SGIIQATELG RIASHFYCTY ESMQTYNKLL VETCSDIDLF RIFSMSSEFK LLSVRDEEKL ELQKMAEHAP IP IKENLDE ASAKTNVLLQ AYISQLKLEG FALQADMVFV AQSAGRLFRA LFEIVLWRGW AGLAQKVLTL CKMVTQRQWG SLN PLHQFK KIPSEVVRSI DKKNYSFDRL YDLDQHQLGD LIKMPKMGKP LFKFIRQFPK LEMTTLIQPI TRTTMRIELT ITPD FKWDE KVHGSAEGFW IFIEDTDGEK ILHHEFFLLK QKFCSDEHVV KMIVPMFDPM PPLYYVRIVS DRWIGAETVL PISFR HLIL PEKYPPPTEL LDLQPLPISA VTNKEFQTVF AESGFKVFNP IQTQVFRTVF ESNENVIVCA PNGSGKTAIA ELAVLR HFE NTPEAKAVYI TPMEDMATKV YADWKRRLEP AIGHTIVLLT GEQTMDLKLA QRGQLIISTP ERWDNISRRW KQRKSVQ NV KLFIADDLHM IGASNGAVFE VVCSRTRYIS SQLESAVRVV ALSSSLTNAR DLGMWLGCSA SATFNFMPST RPVPLDLE I KSFNLSHNAS RFAAMERPVY QAICRHAGKL EPKPALVFVP VRRQTRPVAV ALLTMALADG APKRFLRLAE HDDTFQALL ADIEDESLRE SVSCGVGFLH EGTAPKDVHI VQQLFESNAI QVCVVPRGMC YQIEMSAYLV VVMDTQFYNG KYHVYEDYPI ADMLHMVGL ANRPILDSDA KCVVMCQTSK RAYYKKFLCD PLPVESHLDH CLHDHFNAEI VTKTIENKQD AIDYLTWTLL Y RRMTQNPN YYNLQGTTHR HLSDALSELV ELTLKDLENS KCIAVKDEMD TVSLNLGMIA SYYYISYQTI ELFSMSLKEK TK TRALIEI ISASSEFGNV PMRHKEDVIL RQLAERLPGQ LKNQKFTDPH VKVNLLIHAH LSRVKLTAEL NKDTELIVLR ACR LVQACV DVLSSNGWLS PAIHAMELSQ MLTQAMYSNE PYLKQLPHCS AALLERAKAK EVTSVFELLE LENDDRSDIL QMEG AELAD VARFCNHYPS IEVATELEND VVTSNDNLML AVSLERDNDI DGLAPPVVAP LFPQKRKEEG WWLVIGDSES NALLT IKRL VINEKSSVQL DFAAPRPGHH KFKLFFISDS YLGADQEFDV AFKVEEPGRS NRKRKHEKEE D

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

Macromolecule #6: Tr-type G domain-containing protein

• Macromolecule: Name: Tr-type G domain-containing protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 110.612859 KDa

Sequence

String:

MDSDLYDEFG NYIGPELDSD DDAGDIDDNG DDEDRSDVDE DDEPDRMEED DAEEIPQNQV VLHEDKKYYA TALEVYGEGV ETLVQEEDA QPLTEPIVKP VSKKKFQAAE RFLPETVYKK EYLADLMDCP HIMRNVAIAG HLHHGKTTFL DCLMEQTHPE F YRAEDADA RFTDILFIEK QRGCSIKSQP VSIVAQDSRS KSYLLNIIDT PGHVNFSDEM TASYRLADGV VVMVDAHEGV MM NTERAIR HAIQERLAVT LCISKIDRLL LELKLPPADA YFKLRLIIDQ VNNILSTFAE EDVPVLSPLN GNVIFSSGRY NVC FSLLSF SNIYAKQHGD SFNSKEFARR LWGDIYFEKK TRKFVKKSPS HDAPRTFVQF ILEPMYKIFS QVVGDVDTCL PDVM AELGI RLSKEEQKMN VRPLIALICK RFFGDFSAFV DLVVQNIKSP LENAKTKIEQ TYLGPADSQL AQEMQKCNAE GPLMV HTTK NYPVDDATQF HVFGRVMSGT LEANTDVRVL GENYSIQDEE DCRRMTVGRL FVRVASYQIE VSRVPAGCWV LIEGID QPI VKTATIAELG YEEDVYIFRP LKFNTRSCVK LAVEPINPSE LPKMLDGLRK VNKSYPLLTT RVEESGEHVL LGTGEFY MD CVMHDMRKVF SEIDIKVADP VVTFNETVIE TSTLKCFAET PNKKNKITMM AEPLEKQLDE DIENEVVQIG WNRRRLGE F FQTKYNWDLL AARSIWAFGP DTTGPNILLD DTLPSEVDKH LLSTVRESLV QGFQWATREG PLCEEPIRQV KFKLLDAAI ATEPLYRGGG QMIPTARRCA YSAFLMATPR LMEPYYTVEV VAPADCVAAV YTVLAKRRGH VTTDAPMPGS PMYTISAYIP VMDSFGFET DLRIHTQGQA FCMSAFHHWQ LVPGDPLDKS IVIKTLDVQP TPHLAREFMI KTRRRKGLSE DVSVNKFFDD P MLLELAKQ QDYTGF

UniProtKB: Tr-type G domain-containing protein

Macromolecule #7: Protein isy-1

• Macromolecule: Name: Protein isy-1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 31.326172 KDa

Sequence

String:

MARNAEKAMT ALARWRRMKE EEERGPIARR PHDVKDCRNL SDAERFRREI VRDASKKITA IQNPGLGEFK LRDLNDEVNR LIKLKHAWE QRIRELGGTD YRKYAQKELD AIGRETGNSR GYKYFGAAKD LPGVRELFEK STEGEEQRRH RADLLRNIDA H YFGYLDDE DGRLIPLEKL IEEKNIERIN KEFAEKQAQK QQTASDAAPE NIYKVEEDDD DDLETQESTV IGEDGRPMTI RH VLLPTQQ DIEEMLLEQK KQELMAKYLD

UniProtKB: Protein isy-1

Macromolecule #8: WD_REPEATS_REGION domain-containing protein

• Macromolecule: Name: WD_REPEATS_REGION domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 36.865559 KDa

Sequence

String:

MALVTSSGQQ LVSSGFPQQT AQRFSNLMAP TMVLLGHEGE IYTGAFSPDG TCLATSGYDQ KIFFWNVYGE CENFSTIKGH SGAVMDLKF TTDSSSLVSC GTDKSVRVWD METGTCARRF RTHTDFVNAV HPSRRGVTLV ASASDDGTCR VHDMRTKEPV K TYTNRYQQ TAVTFNDSSD QVISGGIDNV LKVWDMRRDE ITYTLTGHRD TITGISLSPS GKFIISNSMD CTVRQWDIRP FV PGQRSVG VFAGHNHNFE KNLLKCSWSP CERFITAGSS DRFLYVWETL SKKIVYKLPG HMGSVNCTDF HPKEPIMLSC GSD KRVFLG EIDMS

UniProtKB: WD_REPEATS_REGION domain-containing protein

Macromolecule #9: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 99.675094 KDa

Sequence

String:

MADKENATKI EKMPNSETMK GISSEDVPFE EDIIRNPTSV NCWQRYIDHK LQNKSPAKQM FLIYERALAV FERSYKLWYH YLKYRESTI VNKCPTDNSW RALCDTYERC LMRLHKMPRI WICYCEVMIK RGLITETRRV FDRALRSLPV TQHMRIWTLY I GFLTSHDL PETTIRVYRR YLKMNPKARE DYVEYLIERD QIDEAAKELT TLVNQDQNVS EKGRTAHQLW TQLCDLISKN PV KIFSLNV DAIIRQGIYR YTDQVGFLWC SLADYYIRSA EFERARDVYE EAIAKVSTVR DFAQVYDAYA AFEEREVSIM MQE VEQSGD PEEEVDLEWM FQRYQHLMER KNELMNSVLL RQNPHNVGEW LNRVNIYEGN YNKQIETFKE AVKSVNPKIQ VGKV RDLWI GLAKLYEDNG DLDAARKTFE TAVISQFGGV SELANVWCAY AEMEMKHKRA KAALTVMQRA CVVPKPGDYE NMQSV QARV HRSPILWAMY ADYEECCGTV ESCRKVYDKM IELRVASPQM IMNYAMFLEE NEYFELAFQA YEKGIALFKW PGVFDI WNT YLVKFIKRYG GKKLERARDL FEQCLENCPP THAKYIFLLY AKLEEEHGLA RHALSIYNRA CSGVDRADMH SMYNIYI KK VQEMYGIAQC RPIFERAISE LPEDKSRAMS LRYAQLETTV GEIDRARAIY AHAAEISDPK VHVKFWDTWK NFEVAHGN E ATVRDMLRVR RSVEASYNVN VTLTSVQMRV DAERKAQETT TSSNPMDSLD QQQQQPSDGA GSITQVSMNK GNISFVRGA GKTVQQNTTE NPDEIDLDED DDDEEDDGGD ADISVKVVPA QIFGNLKLAE EEEEA

UniProtKB: Pre-mRNA-splicing factor SYF1

Macromolecule #10: TPR_REGION domain-containing protein

• Macromolecule: Name: TPR_REGION domain-containing protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 88.116 KDa

Sequence

String:

MSDDEAAVPG NKPIRLPKKA AKVKNKAPAQ LQITAEQLLR EAKERELELI PPAPKTKITD PDELKEYQRK KRKEFEDGIR KNRMQLANW IKYGKWEESI GEIQRARSVF ERALDVDHRS ISIWLQYAEM EMRCKQINHA RNVFDRAITI MPRAMQFWLK Y SYMEEVIE NIPGARQIFE RWIEWEPPEQ AWQTYINFEL RYKEIDRARS VYQRFLHVHG INVQNWIKYA KFEERNGYIG NA RAAYEKA MEYFGEEDIN ETVLVAFALF EERQKEHERA RGIFKYGLDN LPSNRTEEIF KHYTQHEKKF GERVGIEDVI ISK RKTQYE KMVEENGYNY DAWFDYLRLL ENEETDREEV EDVYERAIAN IPPHSEKRYW RRYIYLWINY ALYEELVAKD FDRA RQVYK ACIDIIPHKT FTFAKVWIMF AHFEIRQLDL NAARKIMGVA IGKCPKDKLF RAYIDLELQL REFDRCRKLY EKFLE SSPE SSQTWIKFAE LETLLGDTDR SRAVFTIAVQ QPALDMPELL WKAYIDFEIA CEEHEKARDL YETLLQRTNH IKVWIS MAE FEQTIGNFEG ARKAFERANQ SLENAEKEER LMLLEAWKEC ETKSGDQEAL KRVETMMPRR VKKRRQIQTE DGVDAGW EE YFDYIFPQDQ AAKGSFKLLE AAARWKRERE EAAARAAQEL DAPIPEGDDD EEKEEAGKDA EEKVREGDSD TDLSESSS S SDSESSSSSS SDSSDSSDDD EDK

UniProtKB: TPR_REGION domain-containing protein

Macromolecule #11: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 27.679885 KDa

Sequence

String:

MSSKPLALTG GSGSSQLQDD QVLVDALPYL DTEYNEADRQ LAMKLVEHEC KTFRPTKNYL THLPVPDYDA FLTKCMLKEM DRMKKKEEM GKLDMSRCEL PAPSAVKGVD RKLWAKVLRN AKAQNEHLLM RQINLELMDE YAAESYLQRN KVMEDLLTHA E KELRKTKE AVMEVHANRK MAQLKAGEKV KQLEQSWVSM VTNNYRMEME NRQIDSDNRK QIKALKLDPT KLDDKEDQEN

UniProtKB: Pre-mRNA-splicing factor SPF27

Macromolecule #12: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 85.843469 KDa

Sequence

String:

MVRVIIKGGV WKNTEDEILK AAIMKYGKNQ WSRIASLLHR KSAKQCKARW FEWLDPGIKK TEWSREEDEK LLHLAKLMPT QWRTIAPIV GRTSAQCLER YEHLLDEAQR KAEGLDEEAT ETRKLKPGEI DPTPETKPAR PDPIDMDDDE LEMLSEARAR L ANTQGKKA KRKARERQLS DARRLASLQK RREMRAAGLA FARKFKPKRN QIDYSEEIPF EKHVPAGFHN PSEDRYVVED AN QKAIEDH QKPRGREIEM EMRREDREKL KKRKEQGEAD AVFNIKEKKR SKLVLPEPQI SDRELEQIVK IGHASDSVRQ YID GTATSG LLTDYTESAR ANAVAARTMR TPMLKDTVQL ELENLMALQN TESALKGGLN TPLHESELGK GVLPTPKVAA TPNT VLHAI AATPGTQSQF PGSTPGGFAT PAGSVAATPF RDQMRINEEI AGSALEQKAS LKRALASLPT PKNDFEVVGP DDDEV EGAV EDESNQDEDG WIEDASERAE NKAKRNAENR VRNMKMRSQV IQRSLPKPTK VNEQATRATN SSADDMVKAE MSKLLA WDV DNKPPSVIYS REELDAAADL IKQEAESGPE LNSLMWKVVE QCTSEIILSK DKFTRIAILP REEQMKALND EFQMYRG WM NQRAKRAAKV EKKLRVKLGG YQAIHDKLCK KYQEVTTEIE MANIEKKTFE RLGEHELKAI NKRVGRLQQE VTTQETRE K DLQKMYSKLS NKQWKLSQIE IHDAASTTSA PITY

UniProtKB: Cell division cycle 5-like protein

Macromolecule #13: Pre-mRNA-splicing factor syf-2

• Macromolecule: Name: Pre-mRNA-splicing factor syf-2 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 27.719021 KDa

Sequence

String:

MSSESQSSSS GPSSSGSKMK DFNQRFRDLH KLRQRARKEN HEQVVEEDRR SKLPKNHEAK KERDQWQVKE LQDRKAAEDK GLDYERVRS LEMSADVTEK LEQKRKRKKN PDQGFTSYED MTLRQHTRLT AALDPDLDSY KKMRECVGGE QFYPTADTLI H GNHYPTTA AMDKLTKDVH GQVKRREQYH RRRLYDPDAP IDYINEKNKK FNKKLDKYYG KYTEDIKDDL ERGTAI

UniProtKB: Pre-mRNA-splicing factor syf-2

Macromolecule #14: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 17.153879 KDa

Sequence

String:

MSLATKLRRV RKSPPEGWDL IEPTLEQFEA KMREAETEPH EGKRKTEINW PIFRIHHQRS RYVYDMYYKK AEISRELYEF CLTAKFADA ALIAKWKKQG YENLCCVKCV NTRDSNFGTA CICRVPKSKL DAERVIECVH CGCHGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #15: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 45.90275 KDa

Sequence

String:

MSMSKSSYSQ YNRKNWEDSD FPILCETCLG NNPYMRMMKD KYGRECKICE RPFTTFRWQP GKGARYKNTE LCQTCAKVKN VCQTCMFDL EYGLPVQVRD HELQIADNIP KQGANRDFFL QNVERTLGQG DGTQPIAQIA NNMDQAAHDR LRRMGRTQPY Y KRNAPHIC SFFVKGECKR GEECPYRHEK PTDPDDPLSR QNIRDRYYGT NDPVAEKILN RAAAAPTLSP PADTTITTLY IG NLGPSGA QQVTEKDLND FFYQYGDIRC LRVLTEKGCA FIEFTTREAA ERAAERSFNK TFIKGKRLTI RWGEPQAKRA ADN SNYVTP VPSVPILPVP DGLAPSTSSQ QRFTGSMPRP PAPPTFAAPR SLVVPNVRPV KAGESSGASS SSSIYYPSQD PTRL GAKGD VIE

UniProtKB: Pre-mRNA-splicing factor RBM22

Macromolecule #16: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 26.154846 KDa

Sequence

String:

MTTAHRPTFH PARGGTARGE GDLSKLSNQY SSKDMPSHTK MKYRQTGQET EADLRKKDLR RELEDKERNA IREKRARDSA SSSSSHSKR QRMDQIAAES AASVDADEAV DELNSSDDDD SDEDDTAALM AELEKIKKER AEEKAARDEE IKEKEEKQRM E NILAGNPL LNDTPAGSST SGGDFTVKRR WDDDVVFKNC AKGVEERKKE VTFINDAIRS EFHKKFMDKY IK

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #17: GCF C-terminal domain-containing protein

• Macromolecule: Name: GCF C-terminal domain-containing protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 94.244891 KDa

Sequence

String:

MFRKPKAKGA IRQRKSDGWD EPDAENQQVV SAIEVKQPAV PRPAMSFDAD EGADSTFKLK KDKKKVEELK RQHKLEEEAE KLYKEEKIR KEALDKIVKK EKLSKEDKKT KNERHKYLDK YRDKSAKHIS NSESYEYEEN LDIDAEAISS VSNKFNSAFE G IPDSRAVF EAKKRRERAR REGNQDGYIP LDDTQKLKSK SERNRLIRED ENDDSDEECT NKFYSARELL RTEEDRRREE QE GFLEREN GDIDEAERIK GDDDSENEEW EKQQIRKAVS RREIGQLRTE KRNTSKLFGH TVPVEDDTAM DMDIDLDMDV QVI GKPEFT GPSNTGGVVK IEDILAKLKL RIQERDEALN FRKEEKRKLE QNIEENKSMI AKIEMELPNQ STKYTMYQEL RVYS RSLLE CLNEKVGEIN SIIDKKRDCG KSRTSRLSVR RRQDMRDQHA ECMQGRNARM GEAAGRAAER DARRGRRRRE REFTL ARIN HEEGLSTDDE EPTPQSMNDQ KICDEVEAVA SVLFADALDE YSDLRKVFGR MTDWLAVDPK SFQDAYVYLC IPKLSS PYV RLQILRADFL RKETILTSMQ WFHIAMLAGS ENAEIDQSHE ILVELAPAIV EKVVIPFLID TVKEEWDPMS LRQTRHL TT FCSLFEKLPN LTEKSKQFNA FLNAIRERIC DCISEDLFMP IFMPNALEQP ICRQFHDRQF WTCIKLIKSI NALSPLIS I AARFELVVEK CVNSQCVMAL RTGSKNDVTA ERKVRGLLAE LDDSLLKMGG RTSFRQLIGT LELIAEEQSK AGRSFHKEI RKFLEKLER

UniProtKB: GCF C-terminal domain-containing protein

Macromolecule #18: Intron-binding protein aquarius

• Macromolecule: Name: Intron-binding protein aquarius / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 170.397688 KDa

Sequence

String:

MVTKRHQEAV VTRGAIENDT ISAVAAKFWA PFTAETHENF DAKLIDTIYD NEMLKTSFNS RKIMMLEFSQ YLEAYLWPNY VPEKASKAW NMSIVVMINE KFRERNLDSW NCFTKKSEHF PHFFKSILQL SLQEEGLASS EHCALLTFLV NAFGSVETPI V HKETRKLV SIEIWAGLLD SQREDLFKKQ KKLKKIWENV RQKMTAAAAD NNEFERTYLW NLIEKFKRVL NSLEPNEAQE SE EGEVRDP IDSIKYCERF IELLIDLESI LQTRRFFNSV LHSSHILTHC LLSSLISTDA GSLFFQLVQL LKFYARFEID DLS GRQLTH KEVSEQHYQS VTRLQKAAFR LFNETMKEFY VLNVSGVDTR RALQKQFGDM NHAEVYRFAE YLHLVPAFGE DPNH QTSLL HLYPHQHLVE TITLHCERRP NQLTQLNEKP LFPTEKVIWD ENIIPYENYT GDGVLALDKL NLQFLTLHDY LLRNF NLFQ LESTYEIRQD LEDVLFRMKP FQHESRNETV FSGWARMALQ IDHFQISEVA KPLVGEKSPA VVRGVVTVNI GRRQDI RQE WENLRKHDVC FLVACRSRKS ASGLKFDVRR PFSEQIEVLS VRGCDVEGML DQDGHLLEEF TAWEKKAKIP GDLRKFR LL LDPNQYRIDM EQGTKDDIYD TFNLIVRRDS KTNNFKAVLQ TIRDLLNTEC VVPDWLTDVI LGYGEPDSAH YSKLSSAV P ELDFNDTFLS FAHVKESFPG YKIELADGFD EKEAVPPFKL EFKELERRQD VEIKPGELRT ILVTPLTRKK VTPYSYDPR KNQVKFTPSQ VEAIKSGMQP GLTMVVGPPG TGKTDVAVQI ISNIYHNWPN QRTLIVTHSN QALNQLFEKI IALDVDERHL LRMGHGEEA LETEKDFSRY GRVNYVLKER LQLLNCVEKL AKALKIVGDV AYTCENAGYF FRFSVCRVWE EFLAKVTSKG C NKLAEGII SEIFPFTGFF KDIPDLFSGN NSADLKVAHS CWRHIEQIFE KLDEFRAFEL LRNGRDRTEY LLVKEAKIIA MT CTHAALR RNELVKLGFR YDNIVMEEAA QILEVETFIP LLLQNPQDGH NRLKRWIMIG DHHQLPPVVQ NQAFQKYSNM EQS LFARLV RLSVPNVQLD RQGRARAQIA ELYQWRYNGL GNLPHVDGLP QFQNANAGFA FPFQFIDIPD FNGHGETQPS PHFY QNLGE AEYACALYTY MRILGYPAEK ISILTTYNGQ AQLIRDVFQR RCDTNPLIGM PAKVSTVDKY QGQQNDFIIL SLVKT RNIG HIRDVRRLVV ALSRARLGLY VLGRSKVFMD CLELTPAMRI FAKYPRKLVI LPFEAHPTIR KWNERSKDGE PMEIQD TLH MTHFVHEFYM SNLPAMRDAY EQAMNEYMES QRLLNPPIDE TQMDVETEHE KKHREAMERK KKQEMDDKKE ADIHFED MD HEMQEPAATA APAPGAPAVE EPPPK

UniProtKB: Intron-binding protein aquarius

Macromolecule #19: Uncharacterized protein T27F2.1

• Macromolecule: Name: Uncharacterized protein T27F2.1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 60.303516 KDa

Sequence

String:

MSMKLRDILP APVAADEAAS QIRRDPWFGG RDNEPSAALV SKEPPPYGKR TSFRPRGPED FGDGGAFPEI HVAQFPLGLG LGDMRGKPE NTLALQYGTD GKLQHDAIAR IGHVKDKVVY SKLNDMKAKT WNEDDDDIQK PDDDAVIDAT EKTRMALEKI V NSKVASAL PVRHADKLAP AQYIRYTPSQ QNGAAGSQQR IIRMVEEQKD PMEPPKFKIN QKIPRAPPSP PAPVMHSPPR KM TAKDQND WKIPPCISNW KNPKGFTVGL DKRLAADGRG LQQTHINENF AKLADALYIA DRKAREEVET RAQLERRVAQ NKK SEQEAK MAEAAAKARQ ERSAMRRKDD EDDEQVKVRE EIRRDRLDDI RKERNIARSR PDKADKLRKE RERDISEKIV LGLP DTNQK RTGEPQFDQR LFDKTQGLDS GAMDDDTYNP YDAAWRGGDS VQQHVYRPSK NLDNDVYGGD LDKIIEQKNR FVADK GFSG AEGSSRGSGP VQFEKDQDVF GLSSLFEHTK EKKRGGDGGD SRGESKRSRR D

UniProtKB: Uncharacterized protein T27F2.1

Macromolecule #20: Peptidyl-prolyl cis-trans isomerase

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 18.547002 KDa

Sequence

String:

MPAPINDQAP YVILDTTMGK IALELYWNHA PRTCQNFSQL AKRNYYNGTI FHRIIADFMI QGGDPTGTGR GGASIYGDKF SDEIDERLK HTGAGILSMA NAGPNTNGSQ FFITLAPTQH LDGKHTIFGR VAAGMKVIAN MGRVDTDNHD RPKIEIRILK A YPSESSVL S

UniProtKB: Peptidyl-prolyl cis-trans isomerase

Macromolecule #21: WD_REPEATS_REGION domain-containing protein

• Macromolecule: Name: WD_REPEATS_REGION domain-containing protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 54.766215 KDa

Sequence

String:

MSASVSDPYE QMPAAPTDDD LEDKPEADKK ALLNQVFKSL KRAQDLFYHD YAQPPPMPEE NDSLIRSMKR KHEYGNVIKK VEEMKVRRE NEMLALPTSQ PMHGTGSVIA SAGTPLAITD GSGKLVNQQQ GSAKSGTLLP LVPLGNSSKG EDNTTRSLLP S KAPMMMKP KWHAPWKLYR VASGHTGWVR AVDVEPGNQW FASGGADRII KIWDLASGQL KLSLTGHISS VRAVKVSPRH PF LFSGGED KQVKCWDLEY NKVIRHYHGH LSAVQALSVH PSLDVLVTCA RDSTARVWDM RTKAQVHCFA GHTNTVADVV CQS VDPQVI TASHDATVRL WDLAAGRSMC TLTHHKKSVR ALTIHPRLNM FASASPDNIK QWKLPKGEFM QNLSGHNAII NTLS SNDDG VVVSGADNGS LCFWDWRSGF CFQKIQTKPQ PGSIESEAGI YASCFDKTGL RLITAEADKT IKMYKEDDEA TEESH PIVW RPEIVKKKAY

UniProtKB: WD_REPEATS_REGION domain-containing protein

Macromolecule #22: Septin and tuftelin-interacting protein 1 homolog

• Macromolecule: Name: Septin and tuftelin-interacting protein 1 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 94.421539 KDa

Sequence

String:

MEDDDGRESF EINDMDLEYA MNPGGRRRFQ NKDQATYGVF APDSDDDDDE QGTSRGPYKK RSKISAPMSF VSGGIQQGNK IDKDDPASL NLNLGGEKKP KEDDEGSIQI DFDKRTKKAP KQNGAQVFAG MRSSANHGAA DINQFGSWMR GDGNSNKIMK M MQAMGYKP GEGLGAQGQG IVEPVQAQLR KGRGAVGAYG KESTATGPKF GESAADAQKR MAQEGTSSRP TNDDQEKSGL KI KGSWKKS QTVKTKYRTI EDVMEEGMSA SRPASHQQSQ QYSNIKVIDM TGKQQKIYSG YDSFSMKTRS EYDTVDDEER TVF DVPELI HNLNLLVDLT EEGIRRSNQQ LISLKDQTTA LEYDLQQVQK SLGTEEQEAQ HIKDVYELID GFSSNRSPSM EECQ ELFRR LRSEFPHEYE LYSLETVAIP TVLPLIQKYF VAWKPLEDKN YGCELISTWR DILDDSKNGR KMTFGHNKTK GDEIR AYDR IIWEGILPSI RRACLQWDPS TQMHEMIELV EQWIPLLSAW ITENILEQLV VPKIAERVNQ WDPMTDEIPI HEWLVP WLV LLGDRIQTVM PPIRQKLSKA LKLWDPMDRS ALETLRPWQN VWSAATFSAF IAQNIVPKLG VALDTMELNP TMNPEYP EW TACMEWLEFT HPDAIANIVT KYFFPRFYNC LCLWLDSPGV DYNEVKRWYG SWKARIPQVL VNYPTVNENL RRSMIAIG R SLQGEKVGGL QATPIAPMAP PPPMAPHFTQ AAPVQKLSLK EIIEYTAGKN GFTYHPQKDR YKDGRQVFWF GALSIYLDS EMVYVMDPIE FVWRPSGLNE LIQMAQGAQG

UniProtKB: Septin and tuftelin-interacting protein 1 homolog

Macromolecule #23: WD_REPEATS_REGION domain-containing protein

• Macromolecule: Name: WD_REPEATS_REGION domain-containing protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 65.385664 KDa

Sequence

String:

MDALQAYGGS DSEHSDDDAS MDQVAKGKSS TLLERAIVTA PDVESKSAIR QVAIVDPKTK EIKSNPKFDQ LFKPESGPVN HFKSEQQRS QKNTLTGFVE PAHLNEFHFN RQIRSFDTLG YAQNPTAESG TTHFVGDVKK AEAEKGVSLF ESKKTGGEKR K RVRNDDSA DIDGYTGPWS RFIDEKTVAK PTPELQKQMD EIVKKRQEKS RRFKKEKEDS EQMAEESSTL HLKEAEDYQG RS FLVPPSF TGVNLREDYV PERCFVPKKL VHTYRGHNKG VNFLQWFPKS AHLFLSCSMD TKIKLWEVYD RQRVVRTYAG HKL PVREVA FNNEGTEFLS ASFDRYVKLW DTETGQVKQR FHTGHVPYCL KYHPDDDKNH MFLVGMQNKK IIQWDSRSGE IVQE YDRHL QAVNSITFFD KNRRFASTSD DKSVRIWEWE IPVDTKLIQN VGLHAIPTMT KSPNDKWVVG QCMDNRIVLF QLVDD KLRF SKKKAFRGHN AAGYACNIDF SPDQSFLISG DADGKLFIWD WRTHKIVGKW KAHDSTCIAA LWHPHEKSRM ITAGWD GLI KMWN

UniProtKB: WD_REPEATS_REGION domain-containing protein

Macromolecule #24: Coiled-coil domain-containing protein 12

• Macromolecule: Name: Coiled-coil domain-containing protein 12 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 19.138486 KDa

Sequence

String:

MTDKNNSDSD EDIESLTNHE TSLEAAAKAR KRRLLAMKSK IHGIEMQEED YDEGETSTKK SREVGREFRN HKPDDAVGTQ NVDMDLDIV QREITEHLKD VLHEKAIDSV DLAMLAPKKI DWDLKRDIES KLQKLERRTQ KAVATIIRQR LAEGKGDLAA T VNAAAAQN L

UniProtKB: Coiled-coil domain-containing protein 12

Macromolecule #25: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 14.836212 KDa

Sequence

String:

MTSVGVPIKI LHEAEGHMVT LETVTGEVYR GKLSEAEDNM NCQLAETVVT FRDGRSHQLD NVFIRGNKIR FMILPDMLKN APMFKNIGR AQKGAIGMGL GGLDQRGRGR GTAFRRPMGR GGPRGMSRPG GAPTFRG

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #26: Probable small nuclear ribonucleoprotein-associated protein B

• Macromolecule: Name: Probable small nuclear ribonucleoprotein-associated protein B; type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 16.768627 KDa

Sequence

String:

MTISKNNKMM AHLNYRMKII LQDGRTFIGF FKAFDKHMNI LLAECEEHRQ IKPKAGKKTD GEEKRILGLV LVRGEHIVSM TVDGPPPRD DDSVRLAKAG GAGGVGQAKP GGRGMPAMPG MPGMPPGGAP GGLSGAMRGH GGPGMAAMQP GYGGPPGGRP F

UniProtKB: Probable small nuclear ribonucleoprotein-associated protein B

Macromolecule #27: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 13.72407 KDa

Sequence

String:

MKLVRFLMKL SHETVNIELK NGTQVSGTIM GVDVAMNTHL RAVSMTVKNK EPVKLDTLSI RGNNIRYIIL PDPLALDTLL IDDEPRKKA RAARAGASRG RGGRGGMRGG RGGRGRGRGG PRGAGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #28: Probable small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Probable small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 13.291529 KDa

Sequence

String:

MSAQAKPRSE MTAEELAAKE DEEFNVGPLS ILTNSVKNNH QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKT GKGKKKAKS VAKDRFISKM FLRGDSVILV VKNPLAQAE

UniProtKB: Probable small nuclear ribonucleoprotein Sm D2

Macromolecule #29: Probable small nuclear ribonucleoprotein E

• Macromolecule: Name: Probable small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 10.625318 KDa

Sequence

String:

MSTRKLNKVM VQPVNLIFRY LQNRTRVQIW LYEDVTHRLE GYIIGFDEFM NVVFDEAEEV NMKTKGRNKI GRILLKGDNI TLIHAAQQE A

UniProtKB: Probable small nuclear ribonucleoprotein E

Macromolecule #30: Probable small nuclear ribonucleoprotein F

• Macromolecule: Name: Probable small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 9.256534 KDa

Sequence

String:

MSAVQPVNPK PFLNSLTGKF VVCKLKWGME YKGVLVAVDS YMNLQLAHAE EYIDGNSQGN LGEILIRCNN VLYVGGVDGE NETSA

UniProtKB: Probable small nuclear ribonucleoprotein F

Macromolecule #31: Probable small nuclear ribonucleoprotein G

• Macromolecule: Name: Probable small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 8.756209 KDa

Sequence

String:

MSKTHPPELK KYMDKEMDLK LNGNRRVSGI LRGFDPFMNM VIDEAVEYQK DGGSVNLGMT VIRGNSVVIM EPKERIS

UniProtKB: Probable small nuclear ribonucleoprotein G

Macromolecule #32: Probable U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: Probable U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 28.9059 KDa

Sequence

String:

MVRLTTELFA ERPQFVNSVN MREINLRGQK IPVIENMGVT RDQFDVIDLT DNDIRKLDNF PTFSRLNTLY LHNNRINYIA PDIATKLPN LKTLALTNNN ICELGDIEPL AECKKLEYVT FIGNPITHKD NYRMYMIYKL PTVRVIDFNR VRLTEREAAK K MFKGKSGK KARDAIQKSV HTEDPSEIEP NENSSGGGAR LTDEDREKIK EAIKNAKSLS EVNYLQSILA SGKVPEKGWN RQ MDQNGAD GEAMES

UniProtKB: Probable U2 small nuclear ribonucleoprotein A'

Macromolecule #33: RRM domain-containing protein

• Macromolecule: Name: RRM domain-containing protein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 24.881344 KDa

Sequence

String:

MADINPNHTI YVNNLNEKVK KDELKRSLHM VFTQFGEIIQ LMSFRKEKMR GQAHIVFKEV SSASNALRAL QGFPFYGKPM RIQYAREDS DVISRAKGTF VEKRQKSTKI AKKPYEKPAK NGKSAAEPTQ KEPQETDGPG LPNNILFCSN IPEGTEPEQI Q TIFSQFPG LREVRWMPNT KDFAFIEYES EDLSEPARQA LDNFRITPTQ QITVKFASK

UniProtKB: RRM domain-containing protein

Macromolecule #34: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 34 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 53.272633 KDa

Sequence

String:

MSFVCGISGE LTEDPVVSQV SGHIFDRRLI VKFIAENGTD PISHGELSED QLVSLKSGGT GSAPRNVSGT SIPSLLKMLQ DEWDTVMLN SFSLRQQLQI ARQELSHSLY QHDAACRVIS RLSKELTAAR EALSTLKPHT SAKVDDDVSI DESEDQQGLS E AILAKLEE KSKSLTAERK QRGKNLPEGL AKTEELAELK QTASHTGIHS TGTPGITALD IKGNLSLTGG IDKTVVLYDY EK EQVMQTF KGHNKKINAV VLHPDNITAI SASADSHIRV WSATDSSSKA IIDVHQAPVT DISLNASGDY ILSASDDSYW AFS DIRSGK SLCKVSVEPG SQIAVHSIEF HPDGLIFGTG AADAVVKIWD LKNQTVAAAF PGHTAAVRSI AFSENGYYLA TGSE DGEVK LWDLRKLKNL KTFANEEKQP INSLSFDMTG TFLGIGGQKV QVLHVKSWSE VVSLSDHSGP VTGVRFGENA RSLVT CSLD KSLRVFSF

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #35: Peptidyl-prolyl cis-trans isomerase E

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase E / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 36.469277 KDa

Sequence

String:

MNTNFPHNRK RTLYVGGFTE DVTEKVLMAA FIPFGDVVAI SIPMDYESGK HRGFGFVEFD MAEDAAMAID NMNESELFGK TIRVNFARP PKATERSQKP VWADDEWLKK YGRGGEAAAE EDGDAEKAAT SSSSASTKLP RVYLGVKIGI RYIGRIVIEL R TDVTPKTA ENFRCLCTGE RGFGYEGSIF HRIIPKFMLQ GGDFTKGDGT GGKSIYGTKF DDENFTLRHT MPGTVSMANC GA NTNGSQF FICTEKTDWL DGKHVVFGHV VEGMNIVRQV EQQGTPSGKP QMVVKIVESG EIEPEKRIAA EKLAQKAVVP GAE IQEPLP QAMET

UniProtKB: Peptidyl-prolyl cis-trans isomerase E

Macromolecule #36: Intron lariat RNA

• Macromolecule: Name: Intron lariat RNA / type: dna / ID: 36 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: Caenorhabditis elegans (invertebrata)
• Molecular weight: Theoretical: 3.680845 KDa

Sequence

String:

(N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)

Macromolecule #37: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 7 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #38: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 38 / Number of copies: 2 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Macromolecule #39: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #40: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 40 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE
• Details: Crosslinked with glutaraledhyde

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.7000000000000001 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6id1

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 879523
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD

Atomic model buiding 1

• Initial model: Chain - Source name: AlphaFold / Chain - Initial model type: in silico model
• Refinement: Protocol: AB INITIO MODEL

Output model

PDB-8ro0:
Structure of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')