EMDB-19399: Integrative Structure of the human intron lariat Spliceosome (ILS'')

Basic information

Entry

Database: EMDB / ID: EMD-19399

• Title: Integrative Structure of the human intron lariat Spliceosome (ILS'')
• Map data: composite map

Sample

• Complex: Human Intron-lariat splicoesome
  • RNA: x 4 types
  • Protein or peptide: x 33 types
• Ligand: x 3 types

• Keywords: mRNA / splicing / intron lariat spliceosome / pre-mRNA

Function / homology

Function:

RNA lariat debranching enzyme activator activity / : / post-spliceosomal complex / negative regulation of double-strand break repair via nonhomologous end joining / U2-type post-mRNA release spliceosomal complex / biomineral tissue development / spliceosomal complex disassembly / protection from non-homologous end joining at telomere / regulation of skeletal muscle satellite cell proliferation / positive regulation of myoblast proliferation / post-mRNA release spliceosomal complex / regulation of retinoic acid receptor signaling pathway / 3'-5' RNA helicase activity / U2 snRNP binding / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / generation of catalytic spliceosome for first transesterification step / histone methyltransferase binding / regulation of vitamin D receptor signaling pathway / histone pre-mRNA 3'end processing complex / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / protein methylation / embryonic brain development / U12-type spliceosomal complex / methylosome / nuclear retinoic acid receptor binding / 7-methylguanosine cap hypermethylation / positive regulation of androgen receptor activity / Prp19 complex / U1 snRNP binding / snRNP binding / mRNA 3'-end processing / ATP-dependent activity, acting on RNA / pICln-Sm protein complex / RNA splicing, via transesterification reactions / U2-type catalytic step 1 spliceosome / small nuclear ribonucleoprotein complex / pre-mRNA binding / sno(s)RNA-containing ribonucleoprotein complex / SMN-Sm protein complex / response to alkaloid / telomerase RNA binding / spliceosomal tri-snRNP complex / telomerase holoenzyme complex / P granule / positive regulation by host of viral transcription / U2-type precatalytic spliceosome / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / positive regulation of vitamin D receptor signaling pathway / commitment complex / Transport of Mature mRNA derived from an Intron-Containing Transcript / U2-type prespliceosome assembly / nuclear vitamin D receptor binding / U2-type catalytic step 2 spliceosome / Notch binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U4 snRNP / positive regulation of mRNA splicing, via spliceosome / RUNX3 regulates NOTCH signaling / U2 snRNP / NOTCH4 Intracellular Domain Regulates Transcription / RNA Polymerase II Transcription Termination / muscle organ development / U1 snRNP / positive regulation of neurogenesis / ubiquitin-ubiquitin ligase activity / NOTCH3 Intracellular Domain Regulates Transcription / lipid biosynthetic process / WD40-repeat domain binding / U2-type prespliceosome / nuclear androgen receptor binding / snoRNA binding / precatalytic spliceosome / K63-linked polyubiquitin modification-dependent protein binding / cyclosporin A binding / Notch-HLH transcription pathway / generation of catalytic spliceosome for second transesterification step / positive regulation of transforming growth factor beta receptor signaling pathway / Formation of paraxial mesoderm / mRNA Splicing - Minor Pathway / spliceosomal complex assembly / SMAD binding / mitotic G2 DNA damage checkpoint signaling / protein K63-linked ubiquitination / mRNA 3'-splice site recognition / blastocyst development / protein peptidyl-prolyl isomerization / protein localization to nucleus / spliceosomal tri-snRNP complex assembly / transcription-coupled nucleotide-excision repair / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / retinoic acid receptor signaling pathway / U5 snRNA binding / U5 snRNP / positive regulation of G1/S transition of mitotic cell cycle / proteasomal protein catabolic process

Domain/homology:

Intron Large complex component GCFC2-like / Nuclear protein DGCR14/ESS-2 / Tuftelin interacting protein, N-terminal domain / GCF, C-terminal / Septin and tuftelin interacting protein / TFP11/STIP/Ntr1 / GC-rich sequence DNA-binding factor-like protein / Nuclear protein Es2 / Tuftelin interacting protein N terminal / mRNA splicing factor Cwf18-like / cwf18 pre-mRNA splicing factor / Sde2, N-terminal ubiquitin domain / Yeast Splicing regulator sde2, N-terminal ubiquitin domain / Cwf19-like protein, C-terminal domain-2 / Cwf19-like, C-terminal domain-1 / Cwf19-like protein / Protein similar to CwfJ C-terminus 2 / Protein similar to CwfJ C-terminus 1 / DHX15, DEXH-box helicase domain / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / CWF11 family / mRNA splicing factor SYF2 / SYF2 splicing factor / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / Helix hairpin bin domain superfamily / G-patch domain / : / Replication stress response SDE2 C-terminal / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / G-patch domain profile. / Pre-mRNA-processing factor 17 / G-patch domain / glycine rich nucleic binding domain / HAT (Half-A-TPR) repeat / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / : / STL11, N-terminal / U-box domain / : / DNA2/NAM7 helicase, helicase domain / : / AAA domain / DNA2/NAM7-like helicase / WD repeat Prp46/PLRG1-like / BUD31/G10-related, conserved site / G10 protein signature 1. / G10 protein signature 2. / : / : / : / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Myb-like DNA-binding domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / Small ribonucleoprotein associated, SmB/SmN / Small nuclear ribonucleoprotein D1 / U-box domain profile. / Modified RING finger domain / U-box domain / Zinc finger, CCCH-type superfamily / HIT-like superfamily / : / Helicase associated domain (HA2), ratchet-like / zinc finger / Pre-mRNA-splicing factor Syf1-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Snu114, GTP-binding domain / Helicase-associated domain / Helicase associated domain (HA2), winged-helix / Helicase associated domain (HA2) Add an annotation / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / : / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Myb-type HTH DNA-binding domain profile.

Component:

ATP-dependent RNA helicase DHX15 / Pleiotropic regulator 1 / RNA helicase aquarius / Pre-mRNA-processing factor 17 / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-splicing factor SYF2 / Small nuclear ribonucleoprotein-associated proteins B and B' / Protein BUD31 homolog / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / SNW domain-containing protein 1 / 116 kDa U5 small nuclear ribonucleoprotein component / CWF19-like protein 2 / CWF19-like protein 1 / Splicing regulator SDE2 / Pre-mRNA-processing-splicing factor 8 / Coiled-coil domain-containing protein 12 / Splicing factor ESS-2 homolog / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Crooked neck-like protein 1 / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RBM22 / Spliceosome-associated protein CWC15 homolog / Tuftelin-interacting protein 11 / Pre-mRNA-splicing factor ISY1 homolog / Pre-mRNA-processing factor 19 / Peptidyl-prolyl cis-trans isomerase-like 1 / PAX3- and PAX7-binding protein 1

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.5 Å
• Authors: Rothe P / Vorlaender MK / Plaschka C

Funding support

European Union, 1 items

Organization	Grant number	Country
European Research Council (ERC)		European Union

• Citation: Journal: Nature / Year: 2024; Title: Mechanism for the initiation of spliceosome disassembly.; Authors: Matthias K Vorländer / Patricia Rothe / Justus Kleifeld / Eric D Cormack / Lalitha Veleti / Daria Riabov-Bassat / Laura Fin / Alex W Phillips / Luisa Cochella / Clemens Plaschka / ; Abstract: Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome. Recent studies have shed light on spliceosome assembly and remodelling for catalysis, but the mechanism of disassembly remains unclear. Here we report cryo-electron microscopy structures of nematode and human terminal intron lariat spliceosomes along with biochemical and genetic data. Our results uncover how four disassembly factors and the conserved RNA helicase DHX15 initiate spliceosome disassembly. The disassembly factors probe large inner and outer spliceosome surfaces to detect the release of ligated mRNA. Two of these factors, TFIP11 and C19L1, and three general spliceosome subunits, SYF1, SYF2 and SDE2, then dock and activate DHX15 on the catalytic U6 snRNA to initiate disassembly. U6 therefore controls both the start and end of pre-mRNA splicing. Taken together, our results explain the molecular basis of the initiation of canonical spliceosome disassembly and provide a framework to understand general spliceosomal RNA helicase control and the discard of aberrant spliceosomes.

History

Deposition	Jan 11, 2024	-
Header (metadata) release	Sep 18, 2024	-
Map release	Sep 18, 2024	-
Update	Sep 18, 2024	-
Current status	Sep 18, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_19399.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: composite map
• Voxel size: X=Y=Z: 1.2375 Å

Density

Contour Level	By AUTHOR: 0.975
Minimum - Maximum	0.0 - 5.822913
Average (Standard dev.)	0.01616737 (±0.1266561)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 519.75 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Human Intron-lariat splicoesome

• Entire: Name: Human Intron-lariat splicoesome

Components

• Complex: Human Intron-lariat splicoesome
  • RNA: U2 snRNA
  • Protein or peptide: Splicing factor ESS-2 homolog
  • RNA: U5 snRNA
  • RNA: U6 snRNA
  • Protein or peptide: Pre-mRNA-processing-splicing factor 8
  • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
  • Protein or peptide: Pre-mRNA-splicing factor ISY1 homolog
  • Protein or peptide: ATP-dependent RNA helicase DHX15
  • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
  • Protein or peptide: Pre-mRNA-splicing factor SYF1
  • RNA: INTRON
  • Protein or peptide: Crooked neck-like protein 1
  • Protein or peptide: Pre-mRNA-splicing factor SPF27
  • Protein or peptide: Cell division cycle 5-like protein
  • Protein or peptide: CWF19-like protein 1
  • Protein or peptide: CWF19-like protein 2
  • Protein or peptide: Pre-mRNA-splicing factor SYF2
  • Protein or peptide: Protein BUD31 homolog
  • Protein or peptide: Pre-mRNA-splicing factor RBM22
  • Protein or peptide: Spliceosome-associated protein CWC15 homolog
  • Protein or peptide: PAX3- and PAX7-binding protein 1
  • Protein or peptide: Intron-binding protein aquarius
  • Protein or peptide: SNW domain-containing protein 1
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
  • Protein or peptide: Pleiotropic regulator 1
  • Protein or peptide: Tuftelin-interacting protein 11
  • Protein or peptide: Pre-mRNA-processing factor 17
  • Protein or peptide: Coiled-coil domain-containing protein 12
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Small nuclear ribonucleoprotein F
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • Protein or peptide: Pre-mRNA-processing factor 19
  • Protein or peptide: Splicing regulator SDE2
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION
• Ligand: INOSITOL HEXAKISPHOSPHATE

Supramolecule #1: Human Intron-lariat splicoesome

• Supramolecule: Name: Human Intron-lariat splicoesome / type: complex / ID: 1 / Parent: 0 ; Macromolecule list: #1, #25-#26, #2, #27, #3-#6, #28-#29, #7-#8, #30, #9, #31-#32, #10-#11, #33-#34, #12-#14, #35-#36, #15-#17, #37, #18-#24
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.186445 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

GENBANK: GENBANK: X02665.1

Macromolecule #2: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.09827 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

GENBANK: GENBANK: NR_004394.1

Macromolecule #26: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 26 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.908668 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

GENBANK: GENBANK: X01691.1

Macromolecule #29: INTRON

• Macromolecule: Name: INTRON / type: rna / ID: 29 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.153607 KDa

Sequence

String:

(N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N) (N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)

Macromolecule #3: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 109.560625 KDa

Sequence

String:

MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

Macromolecule #4: Pre-mRNA-splicing factor ISY1 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor ISY1 homolog / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.046254 KDa

Sequence

String:

MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEV RIKELGGPDY GKVGPKMLDH EGKEVPGNRG YKYFGAAKDL PGVRELFEKE PLPPPRKTRA ELMKAIDFEY Y GYLDEDDG VIVPLEQEYE KKLRAELVEK WKAEREARLA RGEKEEEEEE EEEINIYAVT EEESDEEGSQ EKGGDDSQQK FI AHVPVPS QQEIEEALVR RKKMELLQKY ASETLQAQSE EARRLLGY

UniProtKB: Pre-mRNA-splicing factor ISY1 homolog

Macromolecule #5: ATP-dependent RNA helicase DHX15

• Macromolecule: Name: ATP-dependent RNA helicase DHX15 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 91.065805 KDa

Sequence

String:

MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSA HSTHSTHSAH STHAGHAGHT SLPQCINPFT NLPHTPRYYD ILKKRLQLPV WEYKDRFTDI LVRHQSFVLV G ETGSGKTT QIPQWCVEYM RSLPGPKRGV ACTQPRRVAA MSVAQRVADE MDVMLGQEVG YSIRFEDCSS AKTILKYMTD GM LLREAMN DPLLERYGVI ILDEAHERTL ATDILMGVLK EVVRQRSDLK VIVMSATLDA GKFQIYFDNC PLLTIPGRTH PVE IFYTPE PERDYLEAAI RTVIQIHMCE EEEGDLLLFL TGQEEIDEAC KRIKREVDDL GPEVGDIKII PLYSTLPPQQ QQRI FEPPP PKKQNGAIGR KVVVSTNIAE TSLTIDGVVF VIDPGFAKQK VYNPRIRVES LLVTAISKAS AQQRAGRAGR TRPGK CFRL YTEKAYKTEM QDNTYPEILR SNLGSVVLQL KKLGIDDLVH FDFMDPPAPE TLMRALELLN YLAALNDDGD LTELGS MMA EFPLDPQLAK MVIASCDYNC SNEVLSITAM LSVPQCFVRP TEAKKAADEA KMRFAHIDGD HLTLLNVYHA FKQNHES VQ WCYDNFINYR SLMSADNVRQ QLSRIMDRFN LPRRSTDFTS RDYYINIRKA LVTGYFMQVA HLERTGHYLT VKDNQVVQ L HPSTVLDHKP EWVLYNEFVL TTKNYIRTCT DIKPEWLVKI APQYYDMSNF PQCEAKRQLD RIIAKLQSKE YSQY

UniProtKB: ATP-dependent RNA helicase DHX15

Macromolecule #6: U5 small nuclear ribonucleoprotein 40 kDa protein

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.359492 KDa

Sequence

String:

MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

Macromolecule #7: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.610008 KDa

Sequence

String:

MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

UniProtKB: Crooked neck-like protein 1

Macromolecule #8: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.16342 KDa

Sequence

String:

MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH I QDLNWQRK NMQLTAGSKL REMESNWVSL VSKNYEIERT IVQLENEIYQ IKQQHGEANK ENIRQDF

UniProtKB: Pre-mRNA-splicing factor SPF27

Macromolecule #9: CWF19-like protein 1

• Macromolecule: Name: CWF19-like protein 1 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.696922 KDa

Sequence

String:

MAQKPLRLLA CGDVEGKFDI LFNRVQAIQK KSGNFDLLLC VGNFFGSTQD AEWEEYKTGI KKAPIQTYVL GANNQETVKY FQDADGCEL AENITYLGRK GIFTGSSGLQ IVYLSGTESL NEPVPGYSFS PKDVSSLRMM LCTTSQFKGV DILLTSPWPK C VGNFGNSS GEVDTKKCGS ALVSSLATGL KPRYHFAALE KTYYERLPYR NHIILQENAQ HATRFIALAN VGNPEKKKYL YA FSIVPMK LMDAAELVKQ PPDVTENPYR KSGQEASIGK QILAPVEESA CQFFFDLNEK QGRKRSSTGR DSKSSPHPKQ PRK PPQPPG PCWFCLASPE VEKHLVVNIG THCYLALAKG GLSDDHVLIL PIGHYQSVVE LSAEVVEEVE KYKATLRRFF KSRG KWCVV FERNYKSHHL QLQVIPVPIS CSTTDDIKDA FITQAQEQQI ELLEIPEHSD IKQIAQPGAA YFYVELDTGE KLFHR IKKN FPLQFGREVL ASEAILNVPD KSDWRQCQIS KEDEETLARR FRKDFEPYDF TLDD

UniProtKB: CWF19-like protein 1

Macromolecule #10: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #11: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.959555 KDa

Sequence

String:

MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLE YGLPIQVRDA GLSFKDDMPK SDVNKEYYTQ NMEREISNSD GTRPVGMLGK ATSTSDMLLK LARTTPYYKR N RPHICSFW VKGECKRGEE CPYRHEKPTD PDDPLADQNI KDRYYGINDP VADKLLKRAS TMPRLDPPED KTITTLYVGG LG DTITETD LRNHFYQFGE IRTITVVQRQ QCAFIQFATR QAAEVAAEKS FNKLIVNGRR LNVKWGRSQA ARGKEKEKDG TTD SGIKLE PVPGLPGALP PPPAAEEEAS ANYFNLPPSG PPAVVNIALP PPPGIAPPPP PGFGPHMFHP MGPPPPFMRA PGPI HYPSQ DPQRMGAHAG KHSSP

UniProtKB: Pre-mRNA-splicing factor RBM22

Macromolecule #12: Intron-binding protein aquarius

• Macromolecule: Name: Intron-binding protein aquarius / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 171.502453 KDa

Sequence

String:

MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKA YLMSICCMVN EKFRENVPAW EIFKKKPDHF PFFFKHILKA ALAETDGEFS LHEQTVLLLF LDHCFNSLEV D LIRSQVQQ LISLPMWMGL QLARLELELK KTPKLRKFWN LIKKNDEKMD PEAREQAYQE RRFLSQLIQK FISVLKSVPL SE PVTMDKV HYCERFIELM IDLEALLPTR RWFNTILDDS HLLVHCYLSN LVRREEDGHL FSQLLDMLKF YTGFEINDQT GNA LTENEM TTIHYDRITS LQRAAFAHFP ELYDFALSNV AEVDTRESLV KFFGPLSSNT LHQVASYLCL LPTLPKNEDT TFDK EFLLE LLVSRHERRI SQIQQLNQMP LYPTEKIIWD ENIVPTEYYS GEGCLALPKL NLQFLTLHDY LLRNFNLFRL ESTYE IRQD IEDSVSRMKP WQSEYGGVVF GGWARMAQPI VAFTVVEVAK PNIGENWPTR VRADVTINLN VRDHIKDEWE GLRKHD VCF LITVRPTKPY GTKFDRRRPF IEQVGLVYVR GCEIQGMLDD KGRVIEDGPE PRPNLRGESR TFRVFLDPNQ YQQDMTN TI QNGAEDVYET FNIIMRRKPK ENNFKAVLET IRNLMNTDCV VPDWLHDIIL GYGDPSSAHY SKMPNQIATL DFNDTFLS I EHLKASFPGH NVKVTVEDPA LQIPPFRITF PVRSGKGKKR KDADVEDEDT EEAKTLIVEP HVIPNRGPYP YNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETE KDFSRYGRVN YVLARRIELL EEVKRLQKSL GVPGDASYTC ETAGYFFLYQ VMSRWEEYIS KVKNKGSTLP D VTEVSTFF PFHEYFANAP QPIFKGRSYE EDMEIAEGCF RHIKKIFTQL EEFRASELLR SGLDRSKYLL VKEAKIIAMT CT HAALKRH DLVKLGFKYD NILMEEAAQI LEIETFIPLL LQNPQDGFSR LKRWIMIGDH HQLPPVIKNM AFQKYSNMEQ SLF TRFVRV GVPTVDLDAQ GRARASLCNL YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY FYQN LGEAE YVVALFMYMC LLGYPADKIS ILTTYNGQKH LIRDIINRRC GNNPLIGRPN KVTTVDRFQG QQNDYILLSL VRTRA VGHL RDVRRLVVAM SRARLGLYIF ARVSLFQNCF ELTPAFSQLT ARPLHLHIIP TEPFPTTRKN GERPSHEVQI IKNMPQ MAN FVYNMYMHLI QTTHHYHQTL LQLPPAMVEE GEEVQNQETE LETEEEAMTV QADIIPSPTD TSCRQETPAF QTDTTPS ET GATSTPEAIP ALSETTPTVV GAVSAPAEAN TPQDATSAPE ETK

UniProtKB: RNA helicase aquarius

Macromolecule #13: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 61.770648 KDa

Sequence

String:

MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA DKLAPAQYIR YTPSQQGVAF NSGAKQRVIR MVEMQKDPME PPRFKINKKI PRGPP(SEP)PPAP VMH(SEP) PSRKM TVKEQQEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKM AQKE KEKHEEKLRE MAQKARERRA GIKTHVEKED GEARERDEIR HDRRKERQHD RNLSRAAPDK RSKLQRNENR DISEVI ALG VPNPRTSNEV QYDQRLFNQS KGMDSGFAGG EDEIYNVYDQ AWRGGKDMAQ SIYRPSKNLD KDMYGDDLEA RIKTNRF VP DKEFSGSDRR QRGREGPVQF EEDPFGLDKF LEEAKQHGGS KRPSDSSRPK EHEHEGKKRR KE

UniProtKB: SNW domain-containing protein 1

Macromolecule #14: Peptidyl-prolyl cis-trans isomerase-like 1

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.257805 KDa

Sequence

String:

MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPD LKFTGAGILA MANAGPDTNG SQFFVTLAPT QWLDGKHTIF GRVCQGIGMV NRVGMVETNS QDRPVDDVKI I KAYPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

Macromolecule #15: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.61218 KDa

Sequence

String:

MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ETGQKKTEKR KKFKENDASN IDGFLGPWAK YVDEKDVAKP SEEEQKELDE ITAKRQKKGK QEEEKPGEEK TI LHVKEMY DYQGRSYLHI PQDVGVNLRS TMPPEKCYLP KKQIHVWSGH TKGVSAVRLF PLSGHLLLSC SMDCKIKLWE VYG ERRCLR TFIGHSKAVR DICFNTAGTQ FLSAAYDRYL KLWDTETGQC ISRFTNRKVP YCVKFNPDED KQNLFVAGMS DKKI VQWDI RSGEIVQEYD RHLGAVNTIV FVDENRRFVS TSDDKSLRVW EWDIPVDFKY IAEPSMHSMP AVTLSPNGKW LACQS MDNQ ILIFGAQNRF RLNKKKIFKG HMVAGYACQV DFSPDMSYVI SGDGNGKLNI WDWKTTKLYS RFKAHDKVCI GAVWHP HET SKVITCGWDG LIKLWD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #16: Coiled-coil domain-containing protein 12

• Macromolecule: Name: Coiled-coil domain-containing protein 12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.22385 KDa

Sequence

String:

MEATTAGVGR LEEEALRRKE RLKALREKTG RKDKEDGEPK TKHLREEEEE GEKHRELRLR NYVPEDEDLK KRRVPQAKPV AVEEKVKEQ LEAAKPEPVI EEVDLANLAP RKPDWDLKRD VAKKLEKLKK RTQRAIAELI RERLKGQEDS LASAVDAATE Q KTCDSD

UniProtKB: Coiled-coil domain-containing protein 12

Macromolecule #17: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.940308 KDa

Sequence

String:

MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #18: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.310653 KDa

Sequence

String:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #19: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.551928 KDa

Sequence

String:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #20: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.817601 KDa

Sequence

String:

MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #21: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.734171 KDa

Sequence

String:

MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

UniProtKB: Small nuclear ribonucleoprotein F

Macromolecule #22: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.508084 KDa

Sequence

String:

MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #23: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 23 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 55.245547 KDa

Sequence

String:

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHS FTLRQQLQTT RQELSHALYQ HDAACRVIAR LTKEVTAARE ALATLKPQAG LIVPQAVPSS QPSVVGAGEP M DLGELVGM TPEIIQKLQD KATVLTTERK KRGKTVPEEL VKPEELSKYR QVASHVGLHS ASIPGILALD LCPSDTNKIL TG GADKNVV VFDKSSEQIL ATLKGHTKKV TSVVFHPSQD LVFSASPDAT IRIWSVPNAS CVQVVRAHES AVTGLSLHAT GDY LLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAF SENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTG VAFG HHAKFIASTG MDRSLKFYSL

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #24: Splicing regulator SDE2

• Macromolecule: Name: Splicing regulator SDE2 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.818027 KDa

Sequence

String:

MAEAAALVWI RGPGFGCKAV RCASGRCTVR DFIHRHCQDQ NVPVENFFVK CNGALINTSD TVQHGAVYSL EPRLCGGKGG FGSMLRALG AQIEKTTNRE ACRDLSGRRL RDVNHEKAMA EWVKQQAERE AEKEQKRLER LQRKLVEPKH CFTSPDYQQQ C HEMAERLE DSVLKGMQAA SSKMVSAEIS ENRKRQWPTK SQTDRGASAG KRRCFWLGME GLETAEGSNS ESSDDDSEEA PS TSGMGFH APKIGSNGVE MAAKFPSGSQ RARVVNTDHG SPEQLQIPVT DSGRHILEDS CAELGESKEH MESRMVTETE ETQ EKKAES KEPIEEEPTG AGLNKDKETE ERTDGERVAE VAPEERENVA VAKLQESQPG NAVIDKETID LLAFTSVAEL ELLG LEKLK CELMALGLKC GGTLQERAAR LFSVRGLAKE QIDPALFAKP LKGKKK

UniProtKB: Splicing regulator SDE2

Macromolecule #25: Splicing factor ESS-2 homolog

• Macromolecule: Name: Splicing factor ESS-2 homolog / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.639953 KDa

Sequence

String:

METPGASASS LLLPAASRPP RKREAGEAGA ATSKQRVLDE EEYIEGLQTV IQRDFFPDVE KLQAQKEYLE AEENGDLERM RQIAIKFGS ALGKMSREPP PPYVTPATFE TPEVHAGTGV VGNKPRPRGR GLEDGEAGEE EEKEPLPSLD VFLSRYTSED N ASFQEIME VAKERSRARH AWLYQAEEEF EKRQKDNLEL PSAEHQAIES SQASVETWKY KAKNSLMYYP EGVPDEEQLF KK PRQVVHK NTRFLRDPFS QALSRCQLQQ AAALNAQHKQ GKVGPDGKEL IPQESPRVGG FGFVATPSPA PGVNESPMMT WGE VENTPL RVEGSETPYV DRTPGPAFKI LEPGRRERLG LKMANEAAAK NRAKKQEALR RVTENLASLT PKGLSPAMSP ALQR LVSRT ASKYTDRALR ASYTPSPARS THLKTPASGL QTPTSTPAPG SATRTPLTQD PASITDNLLQ LPARRKASDF F

UniProtKB: Splicing factor ESS-2 homolog

Macromolecule #27: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 273.97425 KDa

Sequence

String:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

Macromolecule #28: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.148711 KDa

Sequence

String:

MVVMARLSRP ERPDLVFEEE DLPYEEEIMR NQFSVKCWLR YIEFKQGAPK PRLNQLYERA LKLLPCSYKL WYRYLKARRA QVKHRCVTD PAYEDVNNCH ERAFVFMHKM PRLWLDYCQF LMDQGRVTHT RRTFDRALRA LPITQHSRIW PLYLRFLRSH P LPETAVRG YRRFLKLSPE SAEEYIEYLK SSDRLDEAAQ RLATVVNDER FVSKAGKSNY QLWHELCDLI SQNPDKVQSL NV DAIIRGG LTRFTDQLGK LWCSLADYYI RSGHFEKARD VYEEAIRTVM TVRDFTQVFD SYAQFEESMI AAKMETASEL GRE EEDDVD LELRLARFEQ LISRRPLLLN SVLLRQNPHH VHEWHKRVAL HQGRPREIIN TYTEAVQTVD PFKATGKPHT LWVA FAKFY EDNGQLDDAR VILEKATKVN FKQVDDLASV WCQCGELELR HENYDEALRL LRKATALPAR RAEYFDGSEP VQNRV YKSL KVWSMLADLE ESLGTFQSTK AVYDRILDLR IATPQIVINY AMFLEEHKYF EESFKAYERG ISLFKWPNVS DIWSTY LTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAA EI YGVTHTRGIY QKAIEVLSDE HAREMCLRFA DMECKLGEID RARAIYSFCS QICDPRTTGA FWQTWKDFEV RHGNEDTI K EMLRIRRSVQ ATYNTQVNFM ASQMLKVSGS ATGTVSDLAP GQSGMDDMKL LEQRAEQLAA EAERDQPLRA QSKILFVRS DASREELAEL AQQVNPEEIQ LGEDEDEDEM DLEPNEVRLE QQSVPAAVFG SLKED

UniProtKB: Pre-mRNA-splicing factor SYF1

Macromolecule #30: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

Macromolecule #31: CWF19-like protein 2

• Macromolecule: Name: CWF19-like protein 2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 103.976492 KDa

Sequence

String:

MATSMAAASG RFESAKSIEE RKEQTRNARA EVLRQAKANF EKEERRKELK RLRGEDTWML PDVNERIEQF SQEHSVKKKK KKDKHSKKA KKEKKKKSKK QKYEKNNESS DSSSSSEDEW VEAVPSQTPD KEKAWKVKDE KSGKDDTQII KRDEWMTVDF M SVKTVSSS SLKAEKETMR KIEQEKNQAL EQSKLMEREL NPYWKDGGTG LPPEDCSVSS ITKVSVVEDG GLSWLRKSYL RM KEQAEKQ SRNFEDIVAE RYGSMEIFQS KLEDAEKAAS TKEDYRRERW RKPTYSDKAQ NCQESRESDL VKYGNSSRDR YAT TDTAKN SNNEKFIGDE KDKRPGSLET CRRESNPRQN QEFSFGNLRA KFLRPSDDEE LSFHSKGRKF EPLSSSSALV AQGS LCSGF RKPTKNSEER LTSWSRSDGR GDKKHSNQKP SETSTDEHQH VPEDPREKSQ DEVLRDDPPK KEHLRDTKST FAGSP ERES IHILSVDEKN KLGAKIIKAE MMGNMELAEQ LKVQLEKANK FKETITQIPK KSGVENEDQQ EVILVRTDQS GRVWPV NTP GKSLESQGGR RKRQMVSTHE ERERVRYFHD DDNLSLNDLV KNEKMGTAEN QNKLFMRMAS KFMGKTDGDY YTLDDMF VS KAAERERLGE EEENQRKKAI AEHRSLAAQM EKCLYCFDSS QFPKHLIVAI GVKVYLCLPN VRSLTEGHCL IVPLQHHR A ATLLDEDIWE EIQMFRKSLV KMFEDKGLDC IFLETNMSMK KQYHMVYECI PLPKEVGDMA PIYFKKAIME SDEEWSMNK KLIDLSSKDI RKSVPRGLPY FSVDFGLHGG FAHVIEDQHK FPHYFGKEII GGMLDIEPRL WRKGIRESFE DQRKKALQFA QWWKPYDFT KSKNY

UniProtKB: CWF19-like protein 2

Macromolecule #32: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.780518 KDa

Sequence

String:

MAAIAASEVL VDSAEEGSLA AAAELAAQKR EQRLRKFREL HLMRNEARKL NHQEVVEEDK RLKLPANWEA KKARLEWELK EEEKKKECA ARGEDYEKVK LLEISAEDAE RWERKKKRKN PDLGFSDYAA AQLRQYHRLT KQIKPDMETY ERLREKHGEE F FPTSNSLL HGTHVPSTEE IDRMVIDLEK QIEKRDKYSR RRPYNDDADI DYINERNAKF NKKAERFYGK YTAEIKQNLE RG TAV

UniProtKB: Pre-mRNA-splicing factor SYF2

Macromolecule #33: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #34: PAX3- and PAX7-binding protein 1

• Macromolecule: Name: PAX3- and PAX7-binding protein 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 104.953656 KDa

Sequence

String:

MFRKARRVNV RKRNDSEEEE RERDEEQEPP PLLPPPGTGE EAGPGGGDRA PGGESLLGPG PSPPSALTPG LGAEAGGGFP GGAEPGNGL KPRKRPRENK EVPRASLLSF QDEEEENEEV FKVKKSSYSK KIVKLLKKEY KEDLEKSKIK TELNSSAESE Q PLDKTGHV KDTNQEDGVI ISEHGEDEMD MESEKEEEKP KTGGAFSNAL SSLNVLRPGE IPDAAFIHAA RKKRQMAREL GD FTPHDNE PGKGRLVRED ENDASDDEDD DEKRRIVFSV KEKSQRQKIA EEIGIEGSDD DALVTGEQDE ELSRWEQEQI RKG INIPQV QASQPAEVNM YYQNTYQTMP YGSSYGIPYS YTAYGSSDAK SQKTDNTVPF KTPSNEMTPV TIDLVKKQLK DRLD SMKEL HKTNRQQHEK HLQSRVDSTR AIERLEGSSG GIGERYKFLQ EMRGYVQDLL ECFSEKVPLI NELESAIHQL YKQRA SRLV QRRQDDIKDE SSEFSSHSNK ALMAPNLDSF GRDRALYQEH AKRRIAEREA RRTRRRQARE QTGKMADHLE GLSSDD EET STDITNFNLE KDRISKESGK VFEDVLESFY SIDCIKSQFE AWRSKYYTSY KDAYIGLCLP KLFNPLIRLQ LLTWTPL EA KCRDFENMLW FESLLFYGCE EREQEKDDVD VALLPTIVEK VILPKLTVIA ENMWDPFSTT QTSRMVGITL KLINGYPS V VNAENKNTQV YLKALLLRMR RTLDDDVFMP LYPKNVLENK NSGPYLFFQR QFWSSVKLLG NFLQWYGIFS NKTLQELSI DGLLNRYILM AFQNSEYGDD SIKKAQNVIN CFPKQWFMNL KGERTISQLE NFCRYLVHLA DTIYRNSIGC SDVEKRNARE NIKQIVKLL ASVRALDHAM SVASDHNVKE FKSLIEGK

UniProtKB: PAX3- and PAX7-binding protein 1

Macromolecule #35: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.280758 KDa

Sequence

String:

MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME TGNTKNSALM AKKAPTMPKP QWHPPWKLYR VISGHLGWVR CIAVEPGNQW FVTGSADRTI KIWDLASGKL KL SLTGHIS TVRGVIVSTR SPYLFSCGED KQVKCWDLEY NKVIRHYHGH LSAVYGLDLH PTIDVLVTCS RDSTARIWDV RTK ASVHTL SGHTNAVATV RCQAAEPQII TGSHDTTIRL WDLVAGKTRV TLTNHKKSVR AVVLHPRHYT FASGSPDNIK QWKF PDGSF IQNLSGHNAI INTLTVNSDG VLVSGADNGT MHLWDWRTGY NFQRVHAAVQ PGSLDSESGI FACAFDQSES RLLTA EADK TIKVYREDDT ATEETHPVSW KPEIIKRKRF

UniProtKB: Pleiotropic regulator 1

Macromolecule #36: Tuftelin-interacting protein 11

• Macromolecule: Name: Tuftelin-interacting protein 11 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 96.945727 KDa

Sequence

String:

MSLSHLYRDG EGRIDDDDDE RENFEITDWD LQNEFNPNRQ RHWQTKEEAT YGVWAERDSD DERPSFGGKR ARDYSAPVNF ISAGLKKGA AEEAELEDSD DEEKPVKQDD FPKDFGPRKL KTGGNFKPSQ KGFAGGTKSF MDFGSWERHT KGIGQKLLQK M GYVPGRGL GKNAQGIINP IEAKQRKGKG AVGAYGSERT TQSMQDFPVV DSEEEAEEEF QKELSQWRKD PSGSKKKPKY SY KTVEELK AKGRISKKLT APQKELSQVK VIDMTGREQK VYYSYSQISH KHNVPDDGLP LQSQQLPQSG KEAKAPGFAL PEL EHNLQL LIDLTEQEII QNDRQLQYER DMVVNLFHEL EKMTEVLDHE ERVISNLSKV LEMVEECERR MQPDCSNPLT LDEC ARIFE TLQDKYYEEY RMSDRVDLAV AIVYPLMKEY FKEWDPLKDC TYGTEIISKW KSLLENDQLL SHGGQDLSAD AFHRL IWEV WMPFVRNIVT QWQPRNCDPM VDFLDSWVHI IPVWILDNIL DQLIFPKLQK EVENWNPLTD TVPIHSWIHP WLPLMQ ARL EPLYSPIRSK LSSALQKWHP SDSSAKLILQ PWKDVFTPGS WEAFMVKNIV PKLGMCLGEL VINPHQQHMD AFYWVID WE GMISVSSLVG LLEKHFFPKW LQVLCSWLSN SPNYEEITKW YLGWKSMFSD QVLAHPSVKD KFNEALDIMN RAVSSNVG A YMQPGARENI AYLTHTERRK DFQYEAMQER REAENMAQRG IGVAASSVPM NFKDLIETKA EEHNIVFMPV IGKRHEGKQ LYTFGRIVIY IDRGVVFVQG EKTWVPTSLQ SLIDMAK

UniProtKB: Tuftelin-interacting protein 11

Macromolecule #37: Small nuclear ribonucleoprotein-associated proteins B and B'

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated proteins B and B'; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.642131 KDa

Sequence

String:

MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

Macromolecule #38: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #39: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 39 / Number of copies: 3 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #40: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 40 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.75 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6id1

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 87951
• Initial angle assignment: Type: OTHER
• Final angle assignment: Type: OTHER