Francesca Vallese / Kookjoo Kim / Laura Y Yen / Jake D Johnston / Alex J Noble / Tito Calì / Oliver Biggs Clarke /
PubMed 要旨
The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association ...The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association with the band 3 anion exchanger and the Rhesus glycoproteins remains unknown. Here we present structures of ankyrin-1 complexes purified from human erythrocytes. We reveal the architecture of a core complex of ankyrin-1, the Rhesus proteins RhAG and RhCE, the band 3 anion exchanger, protein 4.2, glycophorin A and glycophorin B. The distinct T-shaped conformation of membrane-bound ankyrin-1 facilitates recognition of RhCE and, unexpectedly, the water channel aquaporin-1. Together, our results uncover the molecular details of ankyrin-1 association with the erythrocyte membrane, and illustrate the mechanism of ankyrin-mediated membrane protein clustering.
EMDB-26918, PDB-7uzu: Ankyrin-1 (N-terminal region of membrane binding domain, local refinement from consensus reconstruction; bound to N-terminal peptide from band 3) 手法: EM (単粒子) / 解像度: 2.3 Å
EMDB-26958, PDB-8crt: Local refinement of Rh trimer, glycophorin B and Band3-III transmembrane region, class 1a of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.0 Å
EMDB-26972, PDB-8csv: Local refinement of Anykyrin-1 (N-terminal half of membrane binding domain) in Class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.7 Å
EMDB-26978, PDB-8ct2: Local refinement of AQP1 tetramer (C1; refinement mask included D1 of protein 4.2 and Ankyrin-1 AR1-5) in Class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.1 Å
TRANSPORT PROTEIN / Membrane Protein / Anion Exchange / Erythrocyte / Glycoprotein / STRUCTURAL PROTEIN / Ankyrin / TRANSPORT PROTEIN/STRUCTURAL PROTEIN / TRANSPORT PROTEIN-STRUCTURAL PROTEIN complex / ankyrin complex