EMDB-3850: STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFII...

Basic information

• Entry: Database: EMDB / ID: EMD-3850
• Title: STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH AND CORE MEDIATOR
• Map data: Transcription initiation complex including Pol II, TBP, the transcription factors TFIIA, -B, -E, -F and -H and core Mediator

Sample

• Complex: Pre-Initiation Complex with core Mediator (PIC-cMed)
  • Complex: DNA-directed RNA polymerase
    • Protein or peptide: x 13 types
  • Complex: RNA polymerase II transcription factor B
    • Protein or peptide: x 23 types
  • Complex: Mediator of RNA polymerase II transcription
    • Protein or peptide: x 8 types
  • Complex: DNA
    • DNA: x 2 types
• Ligand: x 3 types

• Keywords: transcription initiation / TRANSCRIPTION / macromolecular complex

Function / homology

Function:

meiotic gene conversion / regulation of mitotic recombination / RNA polymerase II promoter clearance / RNA polymerase II complex recruiting activity / TFIIA-class transcription factor complex binding / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / RNA polymerase III transcription regulatory region sequence-specific DNA binding / TFIIH-class transcription factor complex binding / phosphatidylinositol-5-phosphate binding / core mediator complex / positive regulation of mitotic recombination / regulation of transcription by RNA polymerase III / nucleotide-excision repair factor 3 complex / RNA polymerase I general transcription initiation factor binding / transcription factor TFIIE complex / DNA translocase activity / nucleotide-excision repair, preincision complex assembly / negative regulation of ribosomal protein gene transcription by RNA polymerase II / transcription factor TFIIK complex / transcription open complex formation at RNA polymerase II promoter / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / RPB4-RPB7 complex / transcription factor TFIIF complex / DNA 5'-3' helicase / mediator complex / transcription factor TFIIA complex / phosphatidylinositol-3-phosphate binding / RNA polymerase I preinitiation complex assembly / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / cyclin-dependent protein serine/threonine kinase activator activity / DNA 3'-5' helicase / transcription preinitiation complex / DNA binding, bending / poly(A)+ mRNA export from nucleus / RNA Polymerase I Transcription Initiation / DNA duplex unwinding / Processing of Capped Intron-Containing Pre-mRNA / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / RNA Polymerase III Transcription Initiation From Type 2 Promoter / 3'-5' DNA helicase activity / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / TP53 Regulates Transcription of DNA Repair Genes / termination of RNA polymerase II transcription / transcription factor TFIID complex / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA polymerase II general transcription initiation factor activity / RNA-templated transcription / RNA Polymerase II Pre-transcription Events / termination of RNA polymerase III transcription / Formation of TC-NER Pre-Incision Complex / transcription initiation at RNA polymerase III promoter / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / termination of RNA polymerase I transcription / RNA Polymerase I Promoter Escape / nucleolar large rRNA transcription by RNA polymerase I / RNA polymerase II complex binding / protein phosphatase activator activity / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription by RNA polymerase I / transcription initiation at RNA polymerase I promoter / Estrogen-dependent gene expression / TFIID-class transcription factor complex binding / nuclear-transcribed mRNA catabolic process / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / ATPase activator activity / TFIIB-class transcription factor binding / transcription by RNA polymerase III / RNA polymerase II activity / Dual incision in TC-NER / transcription elongation by RNA polymerase I / positive regulation of transcription initiation by RNA polymerase II / transcription-coupled nucleotide-excision repair / tRNA transcription by RNA polymerase III / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase I activity / RNA polymerase I complex / RNA polymerase III complex / positive regulation of translational initiation / translesion synthesis / ATP-dependent activity, acting on DNA / cellular response to nutrient levels / RNA polymerase II, core complex / RNA polymerase II preinitiation complex assembly / translation initiation factor binding / DNA helicase activity / TBP-class protein binding / isomerase activity / DNA-templated transcription initiation / nucleotide-excision repair / transcription initiation at RNA polymerase II promoter / positive regulation of transcription elongation by RNA polymerase II

Domain/homology:

Mediator complex, subunit Med19, fungi / Rox3 mediator complex subunit / Mediator of RNA polymerase II transcription subunit 22, Saccharomycetes / Mediator of RNA polymerase II transcription subunit 20 / Mediator complex, subunit Med6, fungi / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Tfa2 E-tether / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Bacterial type XPD DNA helicase, FeS cluster domain / Med18 protein / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain / TFIIH C1-like domain / TFIIH p62 subunit, N-terminal / TFIIH subunit Tfb1/GTF2H1 / TFIIH p62 subunit, N-terminal domain / BSD domain / BSD domain superfamily / BSD domain / BSD domain profile. / domain in transcription factors and synapse-associated proteins / RAD3/XPD family / Helicase XPB/Ssl2 / ERCC3/RAD25/XPB helicase, C-terminal domain / Helicase XPB/Ssl2, N-terminal domain / Helicase conserved C-terminal domain / ERCC3/RAD25/XPB C-terminal helicase / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Helical and beta-bridge domain / Helical and beta-bridge domain / Transcription factor TFIIH subunit p52/Tfb2 / Transcription factor Tfb2, C-terminal domain / Transcription factor Tfb2 / Transcription factor Tfb2 (p52) C-terminal domain / Transcription factor TFIIE beta subunit, DNA-binding domain / TFIIH subunit TTDA/Tfb5 / Transcription initiation factor TFIIE, beta subunit / TFB5-like superfamily / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / Transcription factor TFIIH complex subunit Tfb5 / TFA2 Winged helix domain 2 / TFIIE beta central core DNA-binding domain profile. / Transcription factor TFIIH complex subunit Tfb5 / ATP-dependent helicase Rad3/Chl1-like / : / Helicase-like, DEXD box c2 type / ATP-dependent helicase, C-terminal / DEAD2 / Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-type / Helicase superfamily 1/2, DinG/Rad3-like / DEAD_2 / Helicase C-terminal domain / Superfamilies 1 and 2 helicase ATP-binding type-2 domain profile. / DEXDc2 / HELICc2 / Transcription initiation factor IIE subunit alpha, N-terminal / Transcription factor TFE/TFIIEalpha HTH domain / TFIIEalpha/SarR/Rpc3 HTH domain / Transcription factor E / TFIIE alpha subunit / TFE/IIEalpha-type HTH domain profile. / Transcription initiation factor IIE

Component:

: / : / DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / Mediator of RNA polymerase II transcription subunit 14 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / Transcription initiation factor IIA large subunit / Transcription initiation factor IIA subunit 2 / General transcription and DNA repair factor IIH subunit TFB1 / Mediator of RNA polymerase II transcription subunit 9 / DNA-directed RNA polymerase II subunit RPB7 / Mediator of RNA polymerase II transcription subunit 20 / Transcription initiation factor IIE subunit alpha / Transcription initiation factor IIE subunit beta / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 6 / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / Mediator of RNA polymerase II transcription subunit 21 / General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 / General transcription and DNA repair factor IIH subunit TFB2 / RNA polymerase II transcription factor B subunit 3 / General transcription and DNA repair factor IIH subunit SSL1 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 7 / General transcription and DNA repair factor IIH subunit TFB4 / Mediator of RNA polymerase II transcription subunit 4 / General transcription and DNA repair factor IIH subunit TFB5 / Mediator of RNA polymerase II transcription subunit 11

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / synthetic construct (others) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 5.8 Å
• Authors: Schilbach S / Hantsche M / Tegunov D / Dienemann C / Wigge C / Urlaub H / Cramer P

Funding support

Germany, 4 items

Organization	Grant number	Country
European Research Council	693023	Germany
German Research Foundation	SFB860	Germany
German Research Foundation	SPP1935	Germany
Volkswagen Foundation		Germany

• Citation: Journal: Nature / Year: 2017; Title: Structures of transcription pre-initiation complex with TFIIH and Mediator.; Authors: S Schilbach / M Hantsche / D Tegunov / C Dienemann / C Wigge / H Urlaub / P Cramer / ; Abstract: For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron microscopy structures of the Saccharomyces cerevisiae PIC and PIC-core Mediator complex at nominal resolutions of 4.7 Å and 5.8 Å, respectively. The structures reveal transcription factor IIH (TFIIH), and suggest how the core and kinase TFIIH modules function in the opening of promoter DNA and the phosphorylation of Pol II, respectively. The TFIIH core subunit Ssl2 (a homologue of human XPB) is positioned on downstream DNA by the 'E-bridge' helix in TFIIE, consistent with TFIIE-stimulated DNA opening. The TFIIH kinase module subunit Tfb3 (MAT1 in human) anchors the kinase Kin28 (CDK7), which is mobile in the PIC but preferentially located between the Mediator hook and shoulder in the PIC-core Mediator complex. Open spaces between the Mediator head and middle modules may allow access of the kinase to its substrate, the C-terminal domain of Pol II.

History

Deposition	Aug 13, 2017	-
Header (metadata) release	May 9, 2018	-
Map release	May 9, 2018	-
Update	May 15, 2024	-
Current status	May 15, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_3850.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Transcription initiation complex including Pol II, TBP, the transcription factors TFIIA, -B, -E, -F and -H and core Mediator
• Voxel size: X=Y=Z: 1.37 Å

Density

Contour Level	By AUTHOR: 0.08 / Movie #1: 0.08
Minimum - Maximum	-0.33997163 - 0.6997163
Average (Standard dev.)	0.0008746041 (±0.024196334)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 350 350 350 Spacing 350 350 350
Cell	A=B=C: 479.5 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.37	1.37	1.37
M x/y/z	350	350	350
origin x/y/z	0.000	0.000	0.000
length x/y/z	479.500	479.500	479.500
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	350	350	350
D min/max/mean	-0.340	0.700	0.001

Supplemental data

Sample components

Entire : Pre-Initiation Complex with core Mediator (PIC-cMed)

• Entire: Name: Pre-Initiation Complex with core Mediator (PIC-cMed)

Components

• Complex: Pre-Initiation Complex with core Mediator (PIC-cMed)
  • Complex: DNA-directed RNA polymerase
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB1
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB4
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC1
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB11
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC4
    • Protein or peptide: Unknown protein
  • Complex: RNA polymerase II transcription factor B
    • Protein or peptide: Transcription initiation factor IIB
    • Protein or peptide: TATA-box-binding protein
    • Protein or peptide: Transcription initiation factor IIF subunit alpha
    • Protein or peptide: Transcription initiation factor IIF subunit beta
    • Protein or peptide: Transcription initiation factor IIA large subunit
    • Protein or peptide: Transcription initiation factor IIA subunit 2
    • Protein or peptide: Transcription factor TFIIE subunit
    • Protein or peptide: Transcription initiation factor IIE subunit beta
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31,Mediator of RNA polymerase II transcription subunit 31
  • Complex: Mediator of RNA polymerase II transcription
    • Protein or peptide: General transcription and DNA repair factor IIH helicase subunit XPD
    • Protein or peptide: General transcription and DNA repair factor IIH subunit TFB1
    • Protein or peptide: General transcription and DNA repair factor IIH subunit TFB2
    • Protein or peptide: RNA polymerase II transcription factor B subunit 3
    • Protein or peptide: General transcription and DNA repair factor IIH subunit TFB4
    • Protein or peptide: General transcription and DNA repair factor IIH subunit TFB5
    • Protein or peptide: General transcription and DNA repair factor IIH subunit SSL1
    • Protein or peptide: General transcription and DNA repair factor IIH helicase subunit XPB
  • Complex: DNA
    • DNA: NONTEMPLATE DNA
    • DNA: TEMPLATE DNA
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: IRON/SULFUR CLUSTER

Supramolecule #1: Pre-Initiation Complex with core Mediator (PIC-cMed)

• Supramolecule: Name: Pre-Initiation Complex with core Mediator (PIC-cMed) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#46
• Molecular weight: Theoretical: 1.95 MDa

Supramolecule #2: DNA-directed RNA polymerase

• Supramolecule: Name: DNA-directed RNA polymerase / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#12, #31
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Supramolecule #3: RNA polymerase II transcription factor B

• Supramolecule: Name: RNA polymerase II transcription factor B / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #13, #15-#17, #19-#22, #32-#46
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Supramolecule #4: Mediator of RNA polymerase II transcription

• Supramolecule: Name: Mediator of RNA polymerase II transcription / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #23-#30
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Supramolecule #5: DNA

• Supramolecule: Name: DNA / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #14, #18
• Source (natural): Organism: synthetic construct (others)

Macromolecule #1: DNA-directed RNA polymerase II subunit RPB1

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 191.821578 KDa

Sequence

String:

MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKP VFHVGFIAKI KKVCECVCMH CGKLLLDEHN ELMRQALAIK DSKKRFAAIW TLCKTKMVCE TDVPSEDDPT Q LVSRGGCG NTQPTIRKDG LKLVGSWKKD RATGDADEPE LRVLSTEEIL NIFKHISVKD FTSLGFNEVF SRPEWMILTC LP VPPPPVR PSISFNESQR GEDDLTFKLA DILKANISLE TLEHNGAPHH AIEEAESLLQ FHVATYMDND IAGQPQALQK SGR PVKSIR ARLKGKEGRI RGNLMGKRVD FSARTVISGD PNLELDQVGV PKSIAKTLTY PEVVTPYNID RLTQLVRNGP NEHP GAKYV IRDSGDRIDL RYSKRAGDIQ LQYGWKVERH IMDNDPVLFN RQPSLHKMSM MAHRVKVIPY STFRLNLSVT SPYNA DFDG DEMNLHVPQS EETRAELSQL CAVPLQIVSP QSNKPCMGIV QDTLCGIRKL TLRDTFIELD QVLNMLYWVP DWDGVI PTP AIIKPKPLWS GKQILSVAIP NGIHLQRFDE GTTLLSPKDN GMLIIDGQII FGVVEKKTVG SSNGGLIHVV TREKGPQ VC AKLFGNIQKV VNFWLLHNGF STGIGDTIAD GPTMREITET IAEAKKKVLD VTKEAQANLL TAKHGMTLRE SFEDNVVR F LNEARDKAGR LAEVNLKDLN NVKQMVMAGS KGSFINIAQM SACVGQQSVE GKRIAFGFVD RTLPHFSKDD YSPESKGFV ENSYLRGLTP QEFFFHAMGG REGLIDTAVK TAETGYIQRR LVKALEDIMV HYDNTTRNSL GNVIQFIYGE DGMDAAHIEK QSLDTIGGS DAAFEKRYRV DLLNTDHTLD PSLLESGSEI LGDLKLQVLL DEEYKQLVKD RKFLREVFVD GEANWPLPVN I RRIIQNAQ QTFHIDHTKP SDLTIKDIVL GVKDLQENLL VLRGKNEIIQ NAQRDAVTLF CCLLRSRLAT RRVLQEYRLT KQ AFDWVLS NIEAQFLRSV VHPGEMVGVL AAQSIGEPAT QMTLNTFHFA GVASKKVTSG VPRLKEILNV AKNMKTPSLT VYL EPGHAA DQEQAKLIRS AIEHTTLKSV TIASEIYYDP DPRSTVIPED EEIIQLHFSL LDEEAEQSFD QQSPWLLRLE LDRA AMNDK DLTMGQVGER IKQTFKNDLF VIWSEDNDEK LIIRCRVVRP KSLDAETEAE EDHMLKKIEN TMLENITLRG VENIE RVVM MKYDRKVPSP TGEYVKEPEW VLETDGVNLS EVMTVPGIDP TRIYTNSFID IMEVLGIEAG RAALYKEVYN VIASDG SYV NYRHMALLVD VMTTQGGLTS VTRHGFNRSN TGALMRCSFE ETVEILFEAG ASAELDDCRG VSENVILGQM APIGTGA FD VMIDEESLVK YMPEQKITEI EDGQDGGVTP YSNESGLVNA DLDVKDELMF SPLVDSGSND AMAGGFTAYG GADYGEAT S PFGAYGEAPT SPGFGVSSPG FSPTSPTYSP TSPAYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPA YSPTSPSYSP TSPSYSPTSP SYSPTSPSY SPTSPNYSPT SPSYSPTSPG YSPGSPAYSP KQDEQKHNEN ENSR

UniProtKB: DNA-directed RNA polymerase II subunit RPB1

Macromolecule #2: DNA-directed RNA polymerase II subunit RPB2

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 138.937297 KDa

Sequence

String:

MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYE ISFGKIYVTK PMVNESDGVT HALYPQEARL RNLTYSSGLF VDVKKRTYEA IDVPGRELKY ELIAEESEDD S ESGKVFIG RLPIMLRSKN CYLSEATESD LYKLKECPFD MGGYFIINGS EKVLIAQERS AGNIVQVFKK AAPSPISHVA EI RSALEKG SRFISTLQVK LYGREGSSAR TIKATLPYIK QDIPIVIIFR ALGIIPDGEI LEHICYDVND WQMLEMLKPC VED GFVIQD RETALDFIGR RGTALGIKKE KRIQYAKDIL QKEFLPHITQ LEGFESRKAF FLGYMINRLL LCALDRKDQD DRDH FGKKR LDLAGPLLAQ LFKTLFKKLT KDIFRYMQRT VEEAHDFNMK LAINAKTITS GLKYALATGN WGEQKKAMSS RAGVS QVLN RYTYSSTLSH LRRTNTPIGR DGKLAKPRQL HNTHWGLVCP AETPEGQACG LVKNLSLMSC ISVGTDPMPI ITFLSE WGM EPLEDYVPHQ SPDATRVFVN GVWHGVHRNP ARLMETLRTL RRKGDINPEV SMIRDIREKE LKIFTDAGRV YRPLFIV ED DESLGHKELK VRKGHIAKLM ATEYQDIEGG FEDVEEYTWS SLLNEGLVEY IDAEEEESIL IAMQPEDLEP AEANEEND L DVDPAKRIRV SHHATTFTHC EIHPSMILGV AASIIPFPDH NQSPRNTYQS AMGKQAMGVF LTNYNVRMDT MANILYYPQ KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGT YDKLDDDGLI APGVRVSGED VIIGKTTPIS PDEEELGQRT AYHSKRDAST PLRSTENGIV DQVLVTTNQD G LKFVKVRV RTTKIPQIGD KFASRHGQKG TIGITYRRED MPFTAEGIVP DLIINPHAIP SRMTVAHLIE CLLSKVAALS GN EGDASPF TDITVEGISK LLREHGYQSR GFEVMYNGHT GKKLMAQIFF GPTYYQRLRH MVDDKIHARA RGPMQVLTRQ PVE GRSRDG GLRFGEMERD CMIAHGAASF LKERLMEASD AFRVHICGIC GLMTVIAKLN HNQFECKGCD NKIDIYQIHI PYAA KLLFQ ELMAMNITPR LYTDRSRDF

UniProtKB: DNA-directed RNA polymerase II subunit RPB2

Macromolecule #3: DNA-directed RNA polymerase II subunit RPB3

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 35.330457 KDa

Sequence

String:

MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCE DHCDKCSVVL TLQAFGESES TTNVYSKDLV IVSNLMGRNI GHPIIQDKEG NGVLICKLRK GQELKLTCVA K KGIAKEHA KWGPAAAIEF EYDPWNKLKH TDYWYEQDSA KEWPQSKNCE YEDPPNEGDP FDYKAQADTF YMNVESVGSI PV DQVVVRG IDTLQKKVAS ILLALTQMDQ DKVNFASGDN NTASNMLGSN EDVMMTGAEQ DPYSNASQMG NTGSGGYDNA W

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #4: DNA-directed RNA polymerase II subunit RPB4

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.451191 KDa

Sequence

String:

MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENAND ETTAVEDEDD DLDEDDVNAD DDDFMHSETR EKELESIDVL LEQTTGGNNK DLKNTMQYLT NFSRFRDQET V GAVIQLLK STGLHPFEVA QLGSLACDTA DEAKTLIPSL NNKISDDELE RILKELSNLE TLY

UniProtKB: DNA-directed RNA polymerase II subunit RPB4

Macromolecule #5: DNA-directed RNA polymerases I, II, and III subunit RPABC1

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.117094 KDa

Sequence

String:

MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVG VKTMKTFVIH IQEKNFQTGI FVYQNNITPS AMKLVPSIPP ATIETFNEAA LVVNITHHEL VPKHIRLSSD E KRELLKRY RLKESQLPRI QRADPVALYL GLKRGEVVKI IRKSETSGRY ASYRICM

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #6: DNA-directed RNA polymerases I, II, and III subunit RPABC2

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC2; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.931834 KDa

Sequence

String:

MSDYEEAFND GNENFEDFDV EHFSDEETYE EKPQFKDGET TDANGKTIVT GGNGPEDFQQ HEQIRRKTLK EKAIPKDQRA TTPYMTKYE RARILGTRAL QISMNAPVFV DLEGETDPLR IAMKELAEKK IPLVIRRYLP DGSFEDWSVE ELIVDL

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #7: DNA-directed RNA polymerase II subunit RPB7

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 19.081053 KDa

Sequence

String:

MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD YDNIDIQRGR ILPTDGSAEF NVKYRAVVFK PFKGEVVDG TVVSCSQHGF EVQVGPMKVF VTKHLMPQDL TFNAGSNPPS YQSSEDVITI KSRIRVKIEG CISQVSSIHA I GSIKEDYL GAI

UniProtKB: DNA-directed RNA polymerase II subunit RPB7

Macromolecule #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.525363 KDa

Sequence

String:

MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSL ADDYDYVMYG TAYKFEEVSK DLIAVYYSFG GLLMRLEGNY RNLNNLKQEN AYLLIRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #9: DNA-directed RNA polymerase II subunit RPB9

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.308161 KDa

Sequence

String:

MTTFRFCRDC NNMLYPREDK ENNRLLFECR TCSYVEEAGS PLVYRHELIT NIGETAGVVQ DIGSDPTLPR SDRECPKCHS RENVFFQSQ QRRKDTSMVL FFVCLSCSHI FTSDQKNKRT QFS

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

Macromolecule #10: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.290732 KDa

Sequence

String:

MIVPVRCFSC GKVVGDKWES YLNLLQEDEL DEGTALSRLG LKRYCCRRMI LTHVDLIEKF LRYNPLEKRD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #11: DNA-directed RNA polymerase II subunit RPB11

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.633493 KDa

Sequence

String:

MNAPDRFELF LLGEGESKLK IDPDTKAPNA VVITFEKEDH TLGNLIRAEL LNDRKVLFAA YKVEHPFFAR FKLRIQTTEG YDPKDALKN ACNSIINKLG ALKTNFETEW NLQTLAADDA F

UniProtKB: DNA-directed RNA polymerase II subunit RPB11

Macromolecule #12: DNA-directed RNA polymerases I, II, and III subunit RPABC4

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC4; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 7.729969 KDa

Sequence

String:

MSREGFQIPT NLDAAAAGTS QARTATLKYI CAECSSKLSL SRTDAVRCKD CGHRILLKAR TKRLVQFEAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Macromolecule #13: Transcription initiation factor IIB

• Macromolecule: Name: Transcription initiation factor IIB / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 38.25734 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDG NNLSTRIGKG ETTDMRFTKE LNKAQGKNVM DKKDNEVQAA FAKITMLCDA AELPKIVKDC AKEAYKLCHD E KTLKGKSM ESIMAASILI GCRRAEVART FKEIQSLIHV KTKEFGKTLN IMKNILRGKS EDGFLKIDTD NMSGAQNLTY IP RFCSHLG LPMQVTTSAE YTAKKCKEIK EIAGKSPITI AVVSIYLNIL LFQIPITAAK VGQTLQVTEG TIKSGYKILY EHR DKLVDP QLIANGVVSL DNLPGVEKK

UniProtKB: Transcription initiation factor IIB

Macromolecule #15: TATA-box-binding protein

• Macromolecule: Name: TATA-box-binding protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.042275 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHA RNAEYNPKRF AAVIMRIREP KTTALIFASG KMVVTGAKSE DDSKLASRKY ARIIQKIGFA AKFTDFKIQN I VGSCDVKF PIRLEGLAFS HGTFSSYEPE LFPGLIYRMV KPKIVLLIFV SGKIVLTGAK QREEIYQAFE AIYPVLSEFR KM

UniProtKB: TATA-box-binding protein

Macromolecule #16: Transcription initiation factor IIF subunit alpha

• Macromolecule: Name: Transcription initiation factor IIF subunit alpha / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 83.341836 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL KFQSKKKINP VTDFHLPVRL HRKDTRNLQF QLTRAEIVQR Q KEISEYKK KAEQERSTPN SGGMNKSGTV SLNNTVKDGS QTPTVDSVTK DNTANGVNSS IPTVTGSSVP PASPTTVSAV ES NGLSNGS TSAANGLDGN ASTANLANGR PLVTKLEDAG PAEDPTKVGM VKYDGKEVTN EPEFEEGTMD PLADVAPDGG GRA KRGNLR RKTRQLKVLD ENAKKLRFEE FYPWVMEDFD GYNTWVGSYE AGNSDSYVLL SVEDDGSFTM IPADKVYKFT ARNK YAT(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) LTIDEAEKRM DKKSGEV PR WLMKHLDNIG TTTTRYDRTR RKLKAVADQQ AMDEDDRDDN SEVELDYDEE FADDEEAPII DGNEQENKES EQRIKKEM L QANAMGLRDE EAPSENEEDE LFGEKKIDED GERIKKALQK TELAALYSSD ENEINPYLSE SDIENKENES PVKKEEDSD TLSKSKRSSP KKQQKKATNA HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS NTVPSPIKQE EGLNSTVAE REETPAPTIT EKDIIEAIGD GKVNIKEFGK FIRRKYPGAE NKKLMFAIVK KLCRKVGNDH MELKKE

UniProtKB: Transcription initiation factor IIF subunit alpha, UNIPROTKB: A0A250WD40

Macromolecule #17: Transcription initiation factor IIF subunit beta

• Macromolecule: Name: Transcription initiation factor IIF subunit beta / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 46.684492 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE EELKKKQQQQ KRRNNRKKFN HRVMTDRDGR DRYIPYVKTI PKKTAIVGTV CHECQVMPSM NDPNYHKIVE QR RNIVKLN NKERITTLDE TVGVTMSHTG MSMRSDNSNF LKVGREKAKS NIKSIRMPKK EILDYLFKLF DEYDYWSLKG LKE RTRQPE AHLKECLDKV ATLVKKGPYA FKYTLRPEYK KLKEEERKAT LGELADEQTG SAGDNAQGDA EADLEDEIEM EDVV

UniProtKB: Transcription initiation factor IIF subunit beta

Macromolecule #19: Transcription initiation factor IIA large subunit

• Macromolecule: Name: Transcription initiation factor IIA large subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 32.230805 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIK EENTGNEGLI LPNINSNNNI PHSGETNINT NTVEATNNSG ATLNTNTSGN TNADVTSQPK IEVKPEIELT I NNANITTV ENIDDESEKK DDEEKEEDVE KTRKEKEQIE QVKLQAKKEK RSALLDTDEV GSELDDSDDD YLISEGEEDG PD ENLMLCL YDKVTRTKAR WKCSLKDGVV TINRNDYTFQ KAQVEAEWV

UniProtKB: Transcription initiation factor IIA large subunit

Macromolecule #20: Transcription initiation factor IIA subunit 2

• Macromolecule: Name: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.47307 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVED SHRDASQNGS GDSQSVISVD KLRIVACNSK KSE

UniProtKB: Transcription initiation factor IIA subunit 2

Macromolecule #21: Transcription factor TFIIE subunit

• Macromolecule: Name: Transcription factor TFIIE subunit / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 59.825934 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYV KYPHAIDAIK WKVHQVVQRL KDDLDKNSEP NGYMCPICLT KYTQLEAVQL LNFDRTEFLC SLCDEPLVED D SGKKNKEK QDKLNRLMDQ IQPIIDSLKK IDDSRI(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)E ENTFEIALAR LIPPQNQSHA AYTYNPKKGS TMFRPGDSAP LPNLMGTALG NDSSRRAGAN SQATLHINI TTASDEVAQR ELQERQAEEK RKQNAVPEWH KQSTIGKTAL GRLDNEEEFD PVVTASAMDS INPDNEPAQE TSYQNNRTLT EQEMEEREN EKTLNDYYAA LAKKQAKLNK EEEEEEEEEE DEEEEEEEEM EDVMDDNDET ARENALEDEF EDVTDTAGTA K TESNTSND VKQESINDKT EDAVNATATA SGPSANAKPN DGDDDDDDDD DEMDIEFEDV

UniProtKB: UNIPROTKB: A0A250WIP3, Transcription initiation factor IIE subunit alpha

Macromolecule #22: Transcription initiation factor IIE subunit beta

• Macromolecule: Name: Transcription initiation factor IIE subunit beta / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 37.050434 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL NSAKNPVLVD IKKEAGSNSS NAISLDDDD DDEDFGSSPS KKVRPGSIAA AALQANQTDI SKSHDSSKLL WATEYIQKKG KPVLVNELLD YLSMKKDDKV I ELLKKLDR IEFDPKKGTF KYLSTYDVHS PSELLKLLRS QVTFKGISCK DLKDGWPQCD ETINQLEEDS KILVLRTKKD KT PRYVWYN SGGNLKCIDE EFVKMWENVQ LPQFAELPRK LQDLGLKPAS VDPATIKRQT KRVEVKKKRQ RKGKITNTHM TGI LKDYSH RV

UniProtKB: Transcription initiation factor IIE subunit beta

Macromolecule #23: General transcription and DNA repair factor IIH helicase subunit XPD

• Macromolecule: Name: General transcription and DNA repair factor IIH helicase subunit XPD; type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 89.064555 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ MHYPEHRKII YCSRTMSEIE KALVELENL MDYRTKELGY QEDFRGLGLT SRKNLCLHPE VSKERKGTVV DEKCRRMTNG QAKRKLEEDP EANVELCEYH E NLYNIEVE DYLPKGVFSF EKLLKYCEEK TLCPYFIVRR MISLCNIIIY SYHYLLDPKI AERVSNEVSK DSIVIFDEAH NI DNVCIES LSLDLTTDAL RRATRGANAL DERISEVRKV DSQKLQDEYE KLVQGL(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)RAEHFVSFL KRLIEYLKTR M KVLHVISE TPKSFLQHLK QLTFIERKPL RFCSERLSLL VRTLEVTEVE DFTALKDIAT FATLISTYEE GFLLIIEPYE IE NAAVPNP IMRFTCLDAS IAIKPVFERF SSVIITSGTI SPLDMYPRML NFKTVLQKSY AMTLAKKSFL PMIITKGSDQ VAI SSRFEI RNDPSIVRNY GSMLVEFAKI TPDGMVVFFP SYLYMESIVS MWQTMGILDE VWKHKLILVE TPDAQETSLA LETY RKACS NGRGAILLSV ARGKVSEGID FDHQYGRTVL MIGIPFQYTE SRILKARLEF MRENYRIREN DFLSFDAMRH AAQCL GRVL RGKDDYGVMV LADRRFSRKR SQLPKWIAQG LSDADLNLST DMAISNTKQF LRTMAQPTDP KDQEGVSVWS YEDLIK HQN SRKDQGGFIE NENKEGEQDE DEDEDIEMQ

UniProtKB: General transcription and DNA repair factor IIH helicase subunit XPD/RAD3, General transcription and DNA repair factor IIH helicase subunit XPD/RAD3

Macromolecule #24: General transcription and DNA repair factor IIH subunit TFB1

• Macromolecule: Name: General transcription and DNA repair factor IIH subunit TFB1; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 53.385336 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMF SFNNRTVMDN IKMTLQQIIS RYKDADIYEE KR(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)TNLKLQQSL LKGNKVLMKV FQETVINAGL PPSEFWSTRI PLLR(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)NLS REKILNIFEN YPIVKKAYTD NVPKNFKEPE FW ARFFSSK LFRK(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)SALGSFVDN T SQANELEV KSTLPIDLLE SCRMLHTTCC EFLKHFYIHF QSGEQKQAST VKKLYNHLKD CIEKLNELFQ DVLNGDGESM SN TCTAYLK PVLNSITLAT HKYDEYFNEY NNNSN

UniProtKB: General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1

Macromolecule #25: General transcription and DNA repair factor IIH subunit TFB2

• Macromolecule: Name: General transcription and DNA repair factor IIH subunit TFB2; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 60.16893 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIP NKSSGTLMIN LNPTFKISLR NAL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)SEKVLNL LKHSKLMEEV NSTGEFKITN EGFQFLLQ(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) KMDLVDVLHF IFMLGALEVG KAYKIDALSE TQRIMLQDMR DYGLVFQKHS NDSIFYPTKL ALMLTSD(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) TKTIRSASNA MDSVLRQNRE EPSVNEDGAN GKSTTDITTS DDLNK AGLK NQDIPDGSLI VETNFKIYSY SNSPLQIAVL SLFVHLKARF VNMVLGQITR ESIRRALTNG ITADQIIAYL ETHAHP QMR RLAEEKLEKK LELDPNCKEP LQVLPPTVVD QIRLWQLELD RVITYEGSLY SDFETSQEYN LLSKYAQDIG VLLWKDD KK KKFFISKEGN SQVLDFAKRK LKKKQ

UniProtKB: General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2

Macromolecule #26: RNA polymerase II transcription factor B subunit 3

• Macromolecule: Name: RNA polymerase II transcription factor B subunit 3 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 38.188438 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVF NVFNKTIDDF NGDLVEYNKY LEEVEDIIYK LDHGIDVAKT EEKLRTYEEL NKQLIMNNLE RSRTEIESFE Q RQKFEKEM KLKKRLLERQ IEEEERMNKE WTKKEIVNRL STTTQDINET IEGVKNTVKL KKSSARRKLE ELNRVLKNNP YF NSNVNVQ NSRLKDAVPF TPFNGDREAH PRFTLKGSVY NDPFIKDLEH RKEFIASGFN TNYAYERVLT EAFMGLGCVI SEE L

UniProtKB: RNA polymerase II transcription factor B subunit 3

Macromolecule #27: General transcription and DNA repair factor IIH subunit TFB4

• Macromolecule: Name: General transcription and DNA repair factor IIH subunit TFB4; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 35.978059 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MDAISDPTFK HARSRKQVTE ESPSLLTVII EIAPKLWTTF DEEGNEKGSI IKVLEALIVF LNAHLAFNSA NKVAVIAAYS QGIKYLYP(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)YRRFRNVDE TLVEEIYKLF ELEKKQIEQN SQRSTLAGAM SAGLTY VNR ISKESVTTSL KSRLLVLTCG SGSSKDEIFQ YIPIMNCIFS ATKMKCPIDV VKIGGSKEST FLQQTTDATN GVYLHVE ST EGLIQYLATA MFIDP(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)TSCYLTG RVVAVGFICS VCLCVLSIIP PGNKCPACDS QFDEHVIAKL KRKPVVPR L KAKKKVTKP

UniProtKB: General transcription and DNA repair factor IIH subunit TFB4, General transcription and DNA repair factor IIH subunit TFB4, General transcription and DNA repair factor IIH subunit TFB4

Macromolecule #28: General transcription and DNA repair factor IIH subunit TFB5

• Macromolecule: Name: General transcription and DNA repair factor IIH subunit TFB5; type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.24349 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MARARKGALV QCDPSIKALI LQIDAKMSDI VLEELDDTHL LVNPSKVEFV KHELNRLLSK NIYNPMDEEE NQ

UniProtKB: General transcription and DNA repair factor IIH subunit TFB5

Macromolecule #29: General transcription and DNA repair factor IIH subunit SSL1

• Macromolecule: Name: General transcription and DNA repair factor IIH subunit SSL1; type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 51.122695 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAPVVISESE EDEDRVAITR RTKRQVHFDG EGDDRVDQQQ QQHSSSHRDR DKHVQRKKKK RLSNRNLQGS NGGYAWEDEI KRSWDLVKV DDEGDMASLV ASIVEARKKR TAKKNITPYQ RGIIRSLILT LDCSEAMLEK DLRPNRHAMI IQYAIDFVHE F FDQNPISQ MGIIIMRNGL AQLVSQVSGN PQDHIDALKS IRKQEPKGNP SLQNALEMAR GLLLPVPAHC TREVLIVFGS LS TTDPGDI HQTIDSLVSE KIRVKVLGLS AQVAICKELC KATNYGDESF YKILLDETHL KELFNEAVTP (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PT RIF EDTPTF CSCHSKLVYG GYFCPNCHSK VCSLPTVCPC CDLMLIL(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)KTFAEVPT TEKFRSEDCF SCQSRFP(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)SRYRCEDCK QEFCVDCDVF IHEILHNCPG CESKPV IT

UniProtKB: General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1

Macromolecule #30: General transcription and DNA repair factor IIH helicase subunit XPB

• Macromolecule: Name: General transcription and DNA repair factor IIH helicase subunit XPB; type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 94.049695 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV TGLKKPRKKT KSSRHTAADS SMNQMDAKD KALLQDTNSD IPADFVPDSV SGMFRSHDFS YLRLRPDHAS RPLWISPSDG RIILESFSPL AEQAQDFLVT I AEPISRPS HIHEYKITAY SLYAAVSVGL ETDDIISVLD RLSKVPVAES IINFIKGATI SYGKVKLVIK HNRYFVETTQ AD ILQMLLN DSVIGPLRID SDHQVQPPED VLQQQLQQTA GKPATNVNPN DVEAVFSAVI GGDNEREEED DDIDAVHSFE IAN ESVEVV KKRCQEIDYP VLEEYDFRND HRNPDLDIDL KPSTQIRPYQ EKSLSKMFGN GRARSGIIVL PCGAGKTLVG ITAA CTIKK SVIVLCTSSV SVMQWRQQFL (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)LVV ST YSMVAN(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)W GFIILDEVHV VPAAMFRRVV STIAAHAKLG LTATLVREDD KIGDLNFLIG PKLY EANWM ELSQKGHIAN VQCAEVWCPM TAEFYQEYLR ETARKRMLLY IMNPTKFQAC QFLIQYHERR GDKIIVFSDN VYALQ EYAL KMGKPFIYGS TPQQERMNIL QNFQYNDQIN TIFLSKVGDT SIDLPEATCL IQISSHYGSR RQEAQRLGRI LRAKRR NDE GFNAFFYSLV SKDTQEMYYS TKRQAFLVDQ GYAFKVITHL HGMENIPNLA YASPRERREL LQEVLLKNEE AAGIEVG DD ADNSVGRGSN GHKRFKSKAV RGEGSLSGLA GGEDMAYMEY STNKNKELKE HHPLIRKMYY KNLKK

UniProtKB: General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2, General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2

Macromolecule #31: Unknown protein

• Macromolecule: Name: Unknown protein / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
• Molecular weight: Theoretical: 3.677524 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #32: Mediator of RNA polymerase II transcription subunit 6

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 32.844746 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPA RRQILFKYPM YMQLEEELMK LDGTEYVLSS VREPDFWVIR KQRRTNNSGV GSAKGPEIIP LQDYYIIGAN I YQSPTIFK IVQSRLMSTS YHLNSTLESL YDLIEFQPSQ GVHYKVPTDT STTATAATNG NNAGGGSNKS SVRPTGGANM AT VPSTTNV NMTVNTMGTG GQTIDNGTGR TGNGNMGITT EMLDKLMVTS IRSTPNYI

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

Macromolecule #33: Mediator of RNA polymerase II transcription subunit 8

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.297234 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQET LDSTVVYPLP KFPTTSHESL VTTLLRKKNI PEVDEWMKYV RETSGVTTAL LKDEEIEKLL QQDREITNWA R TTFRNEYG KHDFKNEESL SEEHASLLVR DSKPSKPFNV DDVLKFTFTG EKPIITGSTS TSSSN

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

Macromolecule #34: Mediator of RNA polymerase II transcription subunit 11

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 11; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.324189 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MQPPYIQERL KSLNDIETQL CSMLQEASQV TFIFGELKRG NESVKPQFEN HVKQFYERLD KSTTQLRKEI QLLDENVGTR LLPINVNKK ALGQDTEKME EQLDLLSAIL DPSKSK

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

Macromolecule #35: Mediator of RNA polymerase II transcription subunit 17

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 17; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 78.582836 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSN PHEIYGSMPL EQLIPIILRQ RGPGFKFVDL NEKELQNEIK QLGSDSSDGH NSEKKDTDGA DENVQIGEDF M EVDYEDKD NPVDSRNETD HKTNENGETD DNIETVMTQE QFVKRRRDML EHINLAMNES SLALEFVSLL LSSVKESTGM SS MSPFLRK VVKPSSLNSD KIPYVAPTKK EYIELDILNK GWKLQSLNES KDLLRASFNK LSSILQNEHD YWNKIMQSIS NKD VIFKIR DRTSGQKLLA IKYGYEDSGS TYKHDRGIAN IRNNIESQNL DLIPHSSSVF KGTDFVHSVK KFLRVRIFTK IESE DDYIL SGESVMDRDS ESEEAETKDI RKQIQLLKKI IFEKELMYQI KKECALLISY GVSIENENKV IIELPNEKFE IELLS LDDD SIVNHEQDLP KINDKRANLM LVMLRLLLVV IFKKTLRSRI SSPHGLINLN VDDDILIIRP ILGKVRFANY KLLLKK IIK DYVLDIVPGS SITETEVERE QPQENKNIDD ENITKLNKEI RAFDKLLNIP RRELKINLPL TEHKSPNLSL MLESPNY CN ALIHIKFSAG TEANAVSFDT TFSDFKEVED FLHFIVAEYI QQKKV

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

Macromolecule #36: Mediator of RNA polymerase II transcription subunit 18

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 18; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.316539 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRI LKSFTNDKIP LNYAMTRNIL HNTVPQVTNF NSTNEDQNNS KHTEDTVNES RNSDDIIDVD MDASPAPSNE S CSPWSLQI SDIPAAGNNR SVSMQTIAET IILSSAGKNS SVSSLMNGLG YVFEFQYLTI GVKFFMKHGL ILELQKIWQI EE AGNSQIT SGGFLLKAYI NVSRGTDIDR INYTETALMN LKKELQGYIE LSVPDRQSMD SRVAHGNILI

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

Macromolecule #37: Mediator of RNA polymerase II transcription subunit 20

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 20; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.918068 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPP ALVFNGSSTG VPESIDTILS SKLSNIWMQR QLIKGDAGET LILDGLTVRL VNLFSSTGFK GLLIELQADE A GEFETKIA GIEGHLAEIR AKEYKTSSDS LGPDTSNEIC DLAYQYVRAL EL

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

Macromolecule #38: Mediator of RNA polymerase II transcription subunit 22

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 22; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.875731 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSNQALYEKL EQTRTILSVK LAELINMTTI ADRNDDDEGS FAQENSELAV ATTSVMMVNN QTMQLIKNVQ DLLILTRSIK EKWLLNQIP VTEHSKVTRF DEKQIEELLD NCIETFVAEK TT

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

Macromolecule #39: Mediator of RNA polymerase II transcription subunit 4

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.606543 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAE YDNIYRNLQK IDKDSEELDS KTRKILEILN ECHDEL(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)KALPMLE QVEFEKNTIL QQRSKINSTE LLDYATKL S KFTKIPPTFD KGAVGPNNFI WPAEDALRRG MLAMASLHSK ELTRIPGEEV EETEVPTVPP SQSEEQKGQM AKKEGTPKT DSFIFDGTAK EVGDEADNTK DKEKEENNDD ALDLDLDLFD PDDF

UniProtKB: Mediator of RNA polymerase II transcription subunit 4, Mediator of RNA polymerase II transcription subunit 4

Macromolecule #40: Mediator of RNA polymerase II transcription subunit 7

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.687055 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSNDPGNEVS SLYSPTSPSY VKFFTQSNLE KLPKYKEKKA ASAKQTAPNN SNGGSEEEIT CALDYLIPPP MPKNQQYRAF GSIWQVKDQ LPDLESMGLT QLYKKSTENE STNYQYKIQE LRKLLKSLLL NYLELIGVLS INPDMYERKV ENIRTILVNI H HLLNEYRP HQSRESLIML LEEQLEYKRG EIREIEQVCK QVHDKLTSIQ DTLRTGSQSP PSSSQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

Macromolecule #41: Mediator of RNA polymerase II transcription subunit 9

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.400377 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MNLQNNVLNQ IHQILLPTNP TLDKPNAEAT KEEFSSAENR DEKDYLTNQQ PKNLSTPSTS SNGEFIPHIF YSLHQIRKDP NNLSNQLET LTGSIRHRLK LCKSLISENE DTKDLLSKSP SEWQDIIHQR EQELQIKRDV LDDLYRKLQR

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

Macromolecule #42: Mediator of RNA polymerase II transcription subunit 10

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 10; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.927926 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIED GRNPDIYTRE FVEAIRRSNQ YQRGKMHGLK QLRDSLADKI VDEFPELKEP VEDIIKRTSP IDNVSNTH

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

Macromolecule #43: Mediator of RNA polymerase II transcription subunit 14

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 14; type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 84.746539 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE SLEMVPKDTS AATMTSAPPP ALPHVEINQ VSLALVIRNL TVFTMKELAQ YMKTNVHTQA NEPNSAKKIR FLQLIIFLRT QFLKLYVLVK WTRTIKQNNF H VLIDLLNW FRTTNMNVNN CIWALKSSLN SMTNAKLPNV DLVTALEVLS LGRPNLPTHN FKLSGVSNSM DMVDGMAKVP IG LILQRLK DLNLTVSIKI ALMNIPKPLN SYHIKNGRIY FTVPNEFEIQ LSTVNRQSPL FFVDLKLLFN TEAEQTVSAV TEA TSTNGD SENNEENSSS NGNNLPLNKP RLEKLINEIL LKSNDPLLSL YNFLHKYVLT LQLYMVHREF LKLANGGKFS KSNL IHNYD SKKSTITVRY WLNGKMDSKG KITIGIQRTT ESLILKWDNQ SASRAKNMPV IYNNIVSNIE GILDEIMFNH ARIIR SELL ARDIFQEDEE NSDVLLFQLP TTCVSMAPIQ LKIDLLSGQF YFRNPTPLLS NYASKINRAE GPEELARILQ QLKLDK IIH VLTTMFENTG WSCSRIIKID KPIRTQVNTG GESVVKKEDN KYAIAGNSTT NSDVSLLLQR DLFIRLPHWP LNWYLIL SI ISSKTSCVVE KRIGKIVSQR GKWNLKYLDN SNVMTVKLES ITYQKIMILQ RTILNRIINH MLIDSLNQLE IRNKICSS E MINEQKLPQY IIQGSNTNDN ISII

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

Macromolecule #44: Mediator of RNA polymerase II transcription subunit 19

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 19; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.885305 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL RKSYKNQIAD LSGKFSTIPT RENGKGGQI AHILFQNNPD MMIQPPQQGQ NMSEQQWREQ LRNRDIALFQ PPNFDWDLCS SVLSQFERSY PSEFANQNQG G AQAPFDID DLAFDLDGTG KSQSGSNSGN NSKKRKNKSS GSSMATPTHS DSHEDMKRRR LE

UniProtKB: Mediator of RNA polymerase II transcription subunit 19

Macromolecule #45: Mediator of RNA polymerase II transcription subunit 21

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 21; type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.093368 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MTDRLTQLQI CLDQMTEQFC ATLNYIDKNH GFERLTVNEP QMSDKHATVV PPEEFSNTID ELSTDIILKT RQINKLIDSL PGVDVSAEE QLRKIDMLQK KLVEVEDEKI EAIKKKEKLL RHVDSLIEDF VDGIANSKKS T

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

Macromolecule #46: Mediator of RNA polymerase II transcription subunit 31,Mediator o...

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 31,Mediator of RNA polymerase II transcription subunit 31; type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
• Molecular weight: Theoretical: 16.790234 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSSTNGNAPA TPSSDQNPLP TRFEVELEFI QSLANIQYVT YLLTQQQIWK SPNFKNYLKY LEYWCNPPYS QCIVYPNCLF ILKLLNGFM ESAIVNEDGL LEGLDELPKI IQLQGPQWMN EMVERWAN(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

Macromolecule #14: NONTEMPLATE DNA

• Macromolecule: Name: NONTEMPLATE DNA / type: dna / ID: 14 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 32.716977 KDa

Sequence

String:

(DC)(DG)(DA)(DG)(DA)(DA)(DC)(DA)(DG)(DT) (DA)(DG)(DC)(DA)(DC)(DG)(DC)(DT)(DG)(DT) (DG)(DT)(DA)(DT)(DA)(DT)(DA)(DA)(DT) (DA)(DG)(DC)(DT)(DA)(DT)(DG)(DG)(DA)(DA) (DC) (DG)(DT)(DT)(DC)(DG)(DA)(DT)(DT) (DC)(DA)(DC)(DC)(DT)(DC)(DC)(DG)(DA)(DT) (DG)(DT) (DG)(DT)(DG)(DT)(DT)(DG)(DT) (DA)(DC)(DA)(DT)(DA)(DC)(DA)(DT)(DA)(DA) (DA)(DA)(DA) (DT)(DA)(DT)(DC)(DA)(DT) (DA)(DG)(DC)(DA)(DC)(DA)(DA)(DC)(DT)(DG) (DC)(DG)(DC)(DT) (DG)(DT)(DG)(DT)(DC) (DA)

Macromolecule #18: TEMPLATE DNA

• Macromolecule: Name: TEMPLATE DNA / type: dna / ID: 18 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 32.68092 KDa

Sequence

String:

(DT)(DG)(DA)(DC)(DA)(DC)(DA)(DG)(DC)(DG) (DC)(DA)(DG)(DT)(DT)(DG)(DT)(DG)(DC)(DT) (DA)(DT)(DG)(DA)(DT)(DA)(DT)(DT)(DT) (DT)(DT)(DA)(DT)(DG)(DT)(DA)(DT)(DG)(DT) (DA) (DC)(DA)(DA)(DC)(DA)(DC)(DA)(DC) (DA)(DT)(DC)(DG)(DG)(DA)(DG)(DG)(DT)(DG) (DA)(DA) (DT)(DC)(DG)(DA)(DA)(DC)(DG) (DT)(DT)(DC)(DC)(DA)(DT)(DA)(DG)(DC)(DT) (DA)(DT)(DT) (DA)(DT)(DA)(DT)(DA)(DC) (DA)(DC)(DA)(DG)(DC)(DG)(DT)(DG)(DC)(DT) (DA)(DC)(DT)(DG) (DT)(DT)(DC)(DT)(DC) (DG)

Macromolecule #47: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 47 / Number of copies: 16 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #48: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 48 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #49: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 49 / Number of copies: 1 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.70 mg/mL
• Buffer: pH: 7.6
• Grid: Model: Quantifoil R1.2/1.3 / Material: GOLD / Pretreatment - Type: GLOW DISCHARGE; Details: QUANTIFOIL HOLEY GOLD GRIDS WERE GLOW-DISCHARGED BEFORE DEPOSITION OF SAMPLE.
• Vitrification: Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV; Details: Grids were blotted for 8 s and plunge-frozen in liquid ethane..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 3 / Number real images: 13000 / Average exposure time: 10.0 sec. / Average electron dose: 42.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 105000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: EMDB MAP
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 16000
• Initial angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0.4)
• Final angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0.4)

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: OTHER

Output model

PDB-5oqm:
STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH AND CORE MEDIATOR