4B6E

Discovery of an allosteric mechanism for the regulation of HCV NS3 protein function

Summary for 4B6E

• Entry DOI: 10.2210/pdb4b6e/pdb
• Related: 1A1Q 1BT7 1C2P 1CSJ 1CU1 1GX5 1GX6 1JXP 1NHU 1NHV 1NS3 1OS5 1QUV 2AWZ 2AX0 2AX1 2BRK 2BRL 2I1R 2JC0 2JC1 2WCX 2WHO 2XWY 4A92 4B6F 8OHM
• Descriptor: NON-STRUCTURAL PROTEIN 4A, SERINE PROTEASE NS3, SULFATE ION, 1H-indazol-7-amine, ... (4 entities in total)
• Functional Keywords: hydrolase, helicase-protease, allosteric pocket, fusion protein
• Biological source: HEPATITIS C VIRUS (ISOLATE BK)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein. Core protein p19: Virion (By similarity). Envelope glycoprotein E1: Virion membrane; Single-pass type I membrane protein (Potential). Envelope glycoprotein E2: Virion membrane; Single-pass type I membrane protein (Potential). p7: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Protease NS2-3: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). RNA-directed RNA polymerase: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential): P26663
• Total number of polymer chains: 2
• Total formula weight: 145257.89

Authors

Saalau-Bethell, S.M.,Woodhead, A.J.,Chessari, G.,Carr, M.G.,Coyle, J.,Graham, B.,Hiscock, S.D.,Murray, C.W.,Pathuri, P.,Rich, S.J.,Richardson, C.J.,Williams, P.A.,Jhoti, H. (deposition date: 2012-08-09, release date: 2012-10-03, Last modification date: 2023-12-20)

• Primary citation: Saalau-Bethell, S.M.,Woodhead, A.J.,Chessari, G.,Carr, M.G.,Coyle, J.,Graham, B.,Hiscock, S.D.,Murray, C.W.,Pathuri, P.,Rich, S.J.,Richardson, C.J.,Williams, P.A.,Jhoti, H.; Discovery of an Allosteric Mechanism for the Regulation of Hcv Ns3 Protein Function.; Nat.Chem.Biol., 8:920-, 2012; PubMed: 23023261; DOI: 10.1038/NCHEMBIO.1081

Experimental method

X-RAY DIFFRACTION (2.46 Å)