6H22

Crystal structure of Mdm2 bound to a stapled peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	E3 ubiquitin-protein ligase Mdm2	polymer	97	11082.0	2	UniProt (Q00987) Pfam (PF02201) In PDB	Homo sapiens (Human)	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
2	C, D	Stapled peptide	polymer	14	1423.6	2		Homo sapiens
3	C, D	12-(dimethylamino)-3,10-diethyl-N,N,N-trimethyl-3,10-dihydrodibenzo[3,4:7,8]cycloocta[1,2-d:5,6-d']bis([1,2,3]triazole)-5-aminium	non-polymer		443.6	2	Chemie (FL5)
4		water	water		18.0	191	Chemie (HOH)

Sequence modifications

A, B: 17 - 108 (UniProt: Q00987)

PDB	External Database	Details
Gly 12	-	expression tag
Pro 13	-	expression tag
Leu 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Ala 69	Glu 69	conflict
Ala 70	Lys 70	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	25011.1
Non-Polymers*	Number of molecules	2
	Total formula weight	887.1
All*	Total formula weight	25898.2

*Water molecules are not included.