5B16

X-ray structure of DROSHA in complex with the C-terminal tail of DGCR8.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ribonuclease 3,DROSHA,Ribonuclease 3,DROSHA,Ribonuclease 3	polymer	986	108687.4	1	UniProt (Q9NRR4) Pfam (PF14622) Pfam (PF00636) Pfam (PF00035) In PDB	Homo sapiens (Human)	Protein Drosha,Ribonuclease III,RNase III,p241,Protein Drosha,Ribonuclease III,RNase III,p241,Protein Drosha,Ribonuclease III,RNase III,p241
2	B, C	Microprocessor complex subunit DGCR8	polymer	39	4591.3	2	UniProt (Q8WYQ5) In PDB	Homo sapiens (Human)	DiGeorge syndrome critical region 8
3	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 411 - 458 (UniProt: Q9NRR4)

PDB	External Database	Details
Met 388	-	initiating methionine
Ala 389	-	expression tag
Lys 390	-	expression tag
Glu 391	-	expression tag
Pro 392	-	expression tag
Glu 393	-	expression tag
Glu 394	-	expression tag
Thr 395	-	expression tag
Met 396	-	expression tag
Pro 397	-	expression tag
Asp 398	-	expression tag
Lys 399	-	expression tag
Asn 400	-	expression tag
Glu 401	-	expression tag
Glu 402	-	expression tag
Glu 403	-	expression tag
Glu 404	-	expression tag
Glu 405	-	expression tag
Glu 406	-	expression tag
Leu 407	-	expression tag
Leu 408	-	expression tag
Lys 409	-	expression tag
Pro 410	-	expression tag

A: 459 - 521 (PDB: 5B16)
A: 522 - 711 (UniProt: Q9NRR4)
A: 712 - 849 (PDB: 5B16)
A: 850 - 1365 (UniProt: Q9NRR4)

PDB	External Database	Details
Gln 1045	Glu 1045	engineered mutation
Gln 1222	Glu 1222	engineered mutation
Ser 1366	-	expression tag
Arg 1367	-	expression tag
Leu 1368	-	expression tag
Glu 1369	-	expression tag
Val 1370	-	expression tag
Leu 1371	-	expression tag
Phe 1372	-	expression tag
Gln 1373	-	expression tag

B, C: 728 - 750 (UniProt: Q8WYQ5)

PDB	External Database	Details
Met 726	-	initiating methionine
Ala 727	-	expression tag
His 751	-	expression tag
Gly 752	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Arg 755	-	expression tag
Gly 756	-	expression tag
Asp 757	-	expression tag
Met 758	-	expression tag
Leu 759	-	expression tag
Glu 760	-	expression tag
Val 761	-	expression tag
Leu 762	-	expression tag
Phe 763	-	expression tag
Gln 764	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	117869.9
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	118000.7

*Water molecules are not included.