5B16
X-ray structure of DROSHA in complex with the C-terminal tail of DGCR8.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2015-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.254, 118.136, 122.296 |
Unit cell angles | 90.00, 102.07, 90.00 |
Refinement procedure
Resolution | 19.930 - 3.200 |
Rwork | 0.267 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.250 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.059 | 0.577 |
Number of reflections | 26854 | |
<I/σ(I)> | 10.7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100mM Citrate Bis-Tris Propane (pH 7.84), 3%(v/v) polyethylene glycol (PEG) 400, 3%(w/v) PEG 20,000, 1%(v/v) ethanol. |